Human Gene GPR21 (uc011lzk.3)
  Description: Homo sapiens G protein-coupled receptor 21 (GPR21), mRNA.
RefSeq Summary (NM_005294): This gene encodes a member of the G-protein-coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. The encoded protein activates a Gq signal transduction pathway which mobilizes calcium. [provided by RefSeq, Nov 2012].
Transcript (Including UTRs)
   Position: hg19 chr9:125,795,922-125,797,944 Size: 2,023 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr9:125,796,846-125,797,895 Size: 1,050 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:125,795,922-125,797,944)mRNA (may differ from genome)Protein (349 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCHPRDLynxMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPR21_HUMAN
DESCRIPTION: RecName: Full=Probable G-protein coupled receptor 21;
FUNCTION: Orphan receptor.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus, hippocampus.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 30.91 RPKM in Artery - Tibial
Total median expression: 790.53 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -168.12496-0.339 Picture PostScript Text
3' UTR -10.0049-0.204 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF13853 - Olfactory receptor

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q99679
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001588 dopamine neurotransmitter receptor activity, coupled via Gs
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007212 dopamine receptor signaling pathway
GO:0040018 positive regulation of multicellular organism growth
GO:0042593 glucose homeostasis
GO:0046627 negative regulation of insulin receptor signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK294531 - Homo sapiens cDNA FLJ53389 complete cds, highly similar to Homo sapiens RAB GTPase activating protein 1 (RABGAP1), mRNA.
AK299686 - Homo sapiens cDNA FLJ60850 complete cds, highly similar to Probable G-protein coupled receptor 21.
AK313539 - Homo sapiens cDNA, FLJ94099, Homo sapiens G protein-coupled receptor 21 (GPR21), mRNA.
CU688178 - Synthetic construct Homo sapiens gateway clone IMAGE:100022925 5' read GPR21 mRNA.
KJ891282 - Synthetic construct Homo sapiens clone ccsbBroadEn_00676 GPR21 gene, encodes complete protein.
AB384939 - Synthetic construct DNA, clone: pF1KB4388, Homo sapiens GPR21 gene for G-protein coupled receptor 21, complete cds, without stop codon, in Flexi system.
BC066885 - Homo sapiens G protein-coupled receptor 21, mRNA (cDNA clone MGC:79437 IMAGE:6971731), complete cds.
BC066886 - Homo sapiens G protein-coupled receptor 21, mRNA (cDNA clone MGC:79440 IMAGE:6971734), complete cds.
BC067532 - Homo sapiens G protein-coupled receptor 21, mRNA (cDNA clone MGC:79438 IMAGE:6971732), complete cds.
BC067533 - Homo sapiens G protein-coupled receptor 21, mRNA (cDNA clone MGC:79439 IMAGE:6971733), complete cds.
BC067534 - Homo sapiens G protein-coupled receptor 21, mRNA (cDNA clone MGC:79441 IMAGE:6971735), complete cds.

-  Other Names for This Gene
  Alternate Gene Symbols: B2R8W9, GPR21_HUMAN, NM_005294, NP_005285, Q6NXU2, Q99679, uc011lzk.2
UCSC ID: uc011lzk.3
RefSeq Accession: NM_005294
Protein: Q99679 (aka GPR21_HUMAN or GP21_HUMAN)
CCDS: CCDS6849.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_005294.2
exon count: 2CDS single in 3' UTR: no RNA size: 1595
ORF size: 1050CDS single in intron: yes Alignment % ID: 100.00
txCdsPredict score: 1964.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.