Human Gene SIK2 (uc001plt.3)
  Description: Homo sapiens salt-inducible kinase 2 (SIK2), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr11:111,473,170-111,597,632 Size: 124,463 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg19 chr11:111,473,288-111,594,853 Size: 121,566 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:111,473,170-111,597,632)mRNA (may differ from genome)Protein (926 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SIK2_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase SIK2; EC=2.7.11.1; AltName: Full=Qin-induced kinase; AltName: Full=Salt-inducible kinase 2; Short=SIK-2; AltName: Full=Serine/threonine-protein kinase SNF1-like kinase 2;
FUNCTION: Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by phosphorylation on Thr-175.
SUBUNIT: Interacts with and phosphorylates TORC2/CRTC2.
INTERACTION: Q9BV73:CEP250; NbExp=5; IntAct=EBI-1181664, EBI-1053100;
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
PTM: Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 UBA domain.
SEQUENCE CAUTION: Sequence=BAA34501.3; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): SIK2
CDC HuGE Published Literature: SIK2
Positive Disease Associations: Coronary Disease
Related Studies:
  1. Coronary Disease
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.33 RPKM in Adipose - Subcutaneous
Total median expression: 251.93 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -53.30118-0.452 Picture PostScript Text
3' UTR -909.582779-0.327 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS
IPR017090 - Ser/Thr_kinase_SIK1/2
IPR015940 - UBA/transl_elong_EF1B_N_euk

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase
PF14531 - Kinase-like

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q9H0K1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
  Protein SequenceProtein Sequence  
  AlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0032870 cellular response to hormone stimulus
GO:0035556 intracellular signal transduction
GO:0046626 regulation of insulin receptor signaling pathway
GO:0046777 protein autophosphorylation

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK291233 - Homo sapiens cDNA FLJ77533 complete cds, highly similar to Homo sapiens SNF1-like kinase 2 (SNF1LK2), mRNA.
AL136764 - Homo sapiens mRNA; cDNA DKFZp434K1115 (from clone DKFZp434K1115).
AB018324 - Homo sapiens KIAA0781 mRNA for KIAA0781 protein.
AK000396 - Homo sapiens cDNA FLJ20389 fis, clone KAIA4591, highly similar to AB018324 Homo sapiens mRNA for KIAA0781 protein.
AB096248 - Homo sapiens LOH11CR1I gene, loss of heterozygosity, 11, chromosomal region 1 gene I product.
BC113459 - Homo sapiens salt-inducible kinase 2, mRNA (cDNA clone MGC:142019 IMAGE:8322511), complete cds.
BC117183 - Homo sapiens salt-inducible kinase 2, mRNA (cDNA clone MGC:150792 IMAGE:40125734), complete cds.
AB383989 - Synthetic construct DNA, clone: pF1KSDA0781, Homo sapiens SNF1LK2 gene for serine/threonine-protein kinase SNF1-like kinase 2, complete cds, without stop codon, in Flexi system.
AB084424 - Homo sapiens mRNA for KIAA0781 protein, partial cds.
DQ571152 - Homo sapiens piRNA piR-31264, complete sequence.
JD476386 - Sequence 457410 from Patent EP1572962.
JD151250 - Sequence 132274 from Patent EP1572962.
JD484654 - Sequence 465678 from Patent EP1572962.
BC078150 - Homo sapiens salt-inducible kinase 2, mRNA (cDNA clone IMAGE:6066794), partial cds.
BC013612 - Homo sapiens salt-inducible kinase 2, mRNA (cDNA clone IMAGE:3882606), partial cds.
DQ582318 - Homo sapiens piRNA piR-32430, complete sequence.
JD176682 - Sequence 157706 from Patent EP1572962.
DQ590070 - Homo sapiens piRNA piR-57182, complete sequence.
JD421003 - Sequence 402027 from Patent EP1572962.
DQ585934 - Homo sapiens piRNA piR-53046, complete sequence.
JD123084 - Sequence 104108 from Patent EP1572962.
JD128593 - Sequence 109617 from Patent EP1572962.
JD056483 - Sequence 37507 from Patent EP1572962.
JD110869 - Sequence 91893 from Patent EP1572962.
JD486641 - Sequence 467665 from Patent EP1572962.
JD238720 - Sequence 219744 from Patent EP1572962.
JD369628 - Sequence 350652 from Patent EP1572962.
JD353521 - Sequence 334545 from Patent EP1572962.
JD315649 - Sequence 296673 from Patent EP1572962.
JD233498 - Sequence 214522 from Patent EP1572962.
JD378776 - Sequence 359800 from Patent EP1572962.
JD551569 - Sequence 532593 from Patent EP1572962.
JD284638 - Sequence 265662 from Patent EP1572962.
DQ570893 - Homo sapiens piRNA piR-31005, complete sequence.
JD499955 - Sequence 480979 from Patent EP1572962.
JD537759 - Sequence 518783 from Patent EP1572962.
JD103688 - Sequence 84712 from Patent EP1572962.
JD273510 - Sequence 254534 from Patent EP1572962.
JD278707 - Sequence 259731 from Patent EP1572962.
JD528260 - Sequence 509284 from Patent EP1572962.
JD218681 - Sequence 199705 from Patent EP1572962.
AK096463 - Homo sapiens cDNA FLJ39144 fis, clone OCBBF1000085.
DQ575531 - Homo sapiens piRNA piR-43643, complete sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: B0YJ94, KIAA0781, NM_015191, NP_056006, O94878, Q17RV0, Q6AZE2, Q76N03, Q8NCV7, Q96CZ8, Q9H0K1, QIK, SIK2_HUMAN, SNF1LK2
UCSC ID: uc001plt.3
RefSeq Accession: NM_015191
Protein: Q9H0K1 (aka SIK2_HUMAN)
CCDS: CCDS8347.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_015191.1
exon count: 15CDS single in 3' UTR: no RNA size: 5694
ORF size: 2781CDS single in intron: no Alignment % ID: 99.96
txCdsPredict score: 5717.00frame shift in genome: no % Coverage: 99.72
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.