Gene interactions and pathways from curated databases and text-mining
J Proteome Res 2003, PMID: 12938929

Multiple functional categories of proteins identified in an in vitro cellular ubiquitin affinity extract using shotgun peptide sequencing.

Gururaja, Tarikere; Li, Weiqun; Noble, William Stafford; Payan, Donald G; Anderson, D C

To construct a high information content assay for examination of the function of the cellular ubiquitin system, we added his-tagged ubiquitin, ATP, and an ATP-regenerating system to endogenous human cellular ubiquitin system enzymes, and labeled cellular proteins with hexa-histidine tagged ubiquitin in vitro. Labeling depended on ATP, the ATP recycling system, the proteasome inhibitor MG132, and the ubiquitin protease inhibitor ubiquitin aldehyde, and was inhibited by iodoacetamide. Quadruplicate affinity extracted proteins were digested with trypsin, and the peptides were analyzed by 2D capillary LC-MS/MS, SEQUEST, MEDUSA, and support vector machine calculations. Identified proteins included 22 proteasome subunits or associated proteins, 18 E1, E2, or E3 ubiquitin system enzymes or related proteins, 4 ubiquitin domain proteins and 36 proteins in functional clusters associated with redox processes, endocytosis/vesicle trafficking, the cytoskeleton, DNA damage/repair, calcium binding, and mRNA splicing. This suggests a link between the ubiquitin system and these cellular processes. This map of cellular ubiquitin-associated proteins may be useful for further studies of ubiquitin system function.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: UBC — EPS15 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RAD23A (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMC2 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: RHOA — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: FAM175A — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD7 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBE2N — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — UBE2T (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — THRAP3 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RPL5 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RIPK2 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — TUFM (direct interaction, pull down)
  • IRef Biogrid Interaction: RNF126 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — BRE (direct interaction, pull down)
  • IRef Biogrid Interaction: AMBRA1 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — CPS1 (direct interaction, pull down)
  • IRef Biogrid Interaction: DOCK7 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: U2SURP — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PDIA4 (direct interaction, pull down)
  • IRef Biogrid Interaction: GID8 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: MRPS17 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD5 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: GIT1 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: FTSJ3 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: OGFR — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — ANKRD10 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RNF20 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RBM23 (direct interaction, pull down)
  • IRef Biogrid Interaction: PLIN3 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: RABEP1 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — TCEB2 (direct interaction, pull down)
  • IRef Biogrid Interaction: SLK — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — TXNDC12 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — MYO9B (direct interaction, pull down)
  • IRef Biogrid Interaction: UBE2S — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — ANXA2 (direct interaction, pull down)
  • IRef Biogrid Interaction: USP11 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — DENND4A (direct interaction, pull down)
  • IRef Biogrid Interaction: UBE2L3 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: CSTB — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — CTTN (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — INF2 (direct interaction, pull down)
  • IRef Biogrid Interaction: RCN2 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — IQGAP1 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — ARHGEF7 (direct interaction, pull down)
  • IRef Biogrid Interaction: SMC6 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: TRIP4 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: TXNDC17 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: ZC3HAV1L — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — DES (direct interaction, pull down)
  • IRef Biogrid Interaction: VTI1A — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: CCAR2 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RCN1 (direct interaction, pull down)
  • IRef Biogrid Interaction: PDIA3 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — RAB11A (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — CEP170 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBE2O — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — CTNND1 (direct interaction, pull down)
  • IRef Biogrid Interaction: TXN2 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBL4A — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD12 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: TUBB — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMD14 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — UBA1 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBR5 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD3 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD4 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: S100A10 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — ATXN3 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMD1 (direct interaction, pull down)
  • IRef Biogrid Interaction: CENPE — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: VCP — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — UCHL5 (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD6 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMC1 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMC4 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBR4 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMB1 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: HUWE1 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMD2 (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMA7 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — SF3B3 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — BAG6 (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMD11 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: PSMA6 — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — HNRNPU (direct interaction, pull down)
  • IRef Biogrid Interaction: SFPQ — UBC (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — PSMC5 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — KRT8 (direct interaction, pull down)
  • IRef Biogrid Interaction: UBC — OTUD5 (direct interaction, pull down)
  • IRef Biogrid Interaction: SSB — UBC (direct interaction, pull down)
In total, 92 gene pairs are associated to this article in curated databases