Gene interactions and pathways from curated databases and text-mining
Carcinogenesis 2007, PMID: 16774940

Reactive oxygen species regulate insulin-induced VEGF and HIF-1alpha expression through the activation of p70S6K1 in human prostate cancer cells.

Zhou, Qiong; Liu, Ling-Zhi; Fu, Beibei; Hu, Xiaowen; Shi, Xianglin; Fang, Jing; Jiang, Bing-Hua

Vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1) are important regulators of angiogenesis. HIF-1 is composed of HIF-1alpha and HIF-1beta subunits, and regulates VEGF expression at transcriptional level. In this study, we demonstrated that insulin induced H2O2 production and p70S6K1 activation in PC-3 prostate cancer cells. The inhibition of H2O2 production by catalase abolished insulin-induced p70S6K1 activation. H2O2 production is also required for insulin-induced VEGF and HIF-1alpha expression in the cells. Over-expression of p70S6K1 or HIF-1alpha reversed catalase- and rapamycin-inhibited VEGF transcriptional activation. These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells. In addition, we found that inhibition of p70S6K1 by rapamycin decreased prostate tumor angiogenesis, suggesting that p70S6K1 plays an important role in tumor angiogenesis. These results provide some useful information for prostate cancer therapy in the future.

Diseases/Pathways annotated by Medline MESH: Neovascularization, Pathologic, Prostatic Neoplasms
Document information provided by NCBI PubMed

Text Mining Data

VEGF → insulin: " Reactive oxygen species regulate insulin induced VEGF and HIF-1alpha expression through the activation of p70S6K1 in human prostate cancer cells "

HIF-1alpha → insulin: " Reactive oxygen species regulate insulin induced VEGF and HIF-1alpha expression through the activation of p70S6K1 in human prostate cancer cells "

p70S6K1 → insulin: " In this study, we demonstrated that insulin induced H2O2 production and p70S6K1 activation in PC-3 prostate cancer cells "

p70S6K1 → insulin: " In this study, we demonstrated that insulin induced H2O2 production and p70S6K1 activation in PC-3 prostate cancer cells "

p70S6K1 → insulin: " The inhibition of H2O2 production by catalase abolished insulin induced p70S6K1 activation "

p70S6K1 → insulin: " The inhibition of H2O2 production by catalase abolished insulin induced p70S6K1 activation "

VEGF → insulin: " H2O2 production is also required for insulin induced VEGF and HIF-1alpha expression in the cells "

HIF-1alpha → insulin: " H2O2 production is also required for insulin induced VEGF and HIF-1alpha expression in the cells "

p70S6K1 → VEGF: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

p70S6K1 → HIF-1alpha: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

p70S6K1 → insulin: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

VEGF → insulin: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

HIF-1alpha → insulin: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

p70S6K1 → VEGF: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

p70S6K1 → HIF-1alpha: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

p70S6K1 → insulin: " These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells "

Manually curated Databases

No curated data.