Gene interactions and pathways from curated databases and text-mining
Nature 2007, PMID: 18046414

mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex.

Cunningham, John T; Rodgers, Joseph T; Arlow, Daniel H; Vazquez, Francisca; Mootha, Vamsi K; Puigserver, Pere

Transcriptional complexes that contain peroxisome-proliferator-activated receptor coactivator (PGC)-1alpha control mitochondrial oxidative function to maintain energy homeostasis in response to nutrient and hormonal signals. An important component in the energy and nutrient pathways is mammalian target of rapamycin (mTOR), a kinase that regulates cell growth, size and survival. However, it is unknown whether and how mTOR controls mitochondrial oxidative activities. Here we show that mTOR is necessary for the maintenance of mitochondrial oxidative function. In skeletal muscle tissues and cells, the mTOR inhibitor rapamycin decreased the gene expression of the mitochondrial transcriptional regulators PGC-1alpha, oestrogen-related receptor alpha and nuclear respiratory factors, resulting in a decrease in mitochondrial gene expression and oxygen consumption. Using computational genomics, we identified the transcription factor yin-yang 1 (YY1) as a common target of mTOR and PGC-1alpha. Knockdown of YY1 caused a significant decrease in mitochondrial gene expression and in respiration, and YY1 was required for rapamycin-dependent repression of those genes. Moreover, mTOR and raptor interacted with YY1, and inhibition of mTOR resulted in a failure of YY1 to interact with and be coactivated by PGC-1alpha. We have therefore identified a mechanism by which a nutrient sensor (mTOR) balances energy metabolism by means of the transcriptional control of mitochondrial oxidative function. These results have important implications for our understanding of how these pathways might be altered in metabolic diseases and cancer.

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Text Mining Data

YY1 ⊣ raptor: " Moreover, mTOR and raptor interacted with YY1, and inhibition of mTOR resulted in a failure of YY1 to interact with and be coactivated by PGC-1alpha "

YY1 ⊣ mTOR: " Moreover, mTOR and raptor interacted with YY1, and inhibition of mTOR resulted in a failure of YY1 to interact with and be coactivated by PGC-1alpha "

raptor ⊣ mTOR: " Moreover, mTOR and raptor interacted with YY1, and inhibition of mTOR resulted in a failure of YY1 to interact with and be coactivated by PGC-1alpha "

Manually curated Databases

  • NCI Pathway Database mTOR signaling pathway: mTORC1 complex (MTOR-MLST8-RPTOR) → YY1 (YY1) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
  • NCI Pathway Database mTOR signaling pathway: mTORC1 complex (MTOR-MLST8-RPTOR) → PGC1A (PPARGC1A) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
  • NCI Pathway Database mTOR signaling pathway: mTORC1 complex (MTOR-MLST8-RPTOR) → mTORC1/PGC1A/YY1 complex (MTOR-MLST8-RPTOR-PPARGC1A-YY1) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
  • NCI Pathway Database mTOR signaling pathway: YY1 (YY1) → PGC1A (PPARGC1A) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
  • NCI Pathway Database mTOR signaling pathway: YY1 (YY1) → mTORC1/PGC1A/YY1 complex (MTOR-MLST8-RPTOR-PPARGC1A-YY1) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
  • NCI Pathway Database mTOR signaling pathway: PGC1A (PPARGC1A) → mTORC1/PGC1A/YY1 complex (MTOR-MLST8-RPTOR-PPARGC1A-YY1) (modification, collaborate)
    Evidence: mutant phenotype, physical interaction
In total, 15 gene pairs are associated to this article in curated databases