Gene interactions and pathways from curated databases and text-mining
Cell Signal 2008, PMID: 18207367

Roles of autophagy and mTOR signaling in neuronal differentiation of mouse neuroblastoma cells.

Zeng, Mei; Zhou, Jiang-Ning

Cell differentiation is often associated with decreased cell growth, indicating an altered rate of macromolecule synthesis and degradation. In this study, we present evidence that autophagy, a process for bulk degradation of cytoplasm, is activated during retinoic acid-induced neuronal differentiation of neuroblastoma N2a cells. Chemical inhibitors of autophagy, including 3-MA and LY294002, abrogate cell differentiation. RNA interference of autophagy gene beclin 1 markedly delays the process of differentiation. We also find that cell differentiation is accompanied by decreased activity of mTOR, a major controller of cell growth and a negative regulator of autophagy. However, completely inhibiting mTOR by rapamycin decreases neurite outgrowth, cell size and the immunoreactivity for neuronal markers. Our study suggests that an appropriate level of mTOR activity is important in cell differentiation for a balance between macromolecule synthesis and degradation.

Diseases/Pathways annotated by Medline MESH: Neuroblastoma
Document information provided by NCBI PubMed

Text Mining Data

neuronal differentiation ⊣ mTOR signaling: " Roles of autophagy and mTOR signaling in neuronal differentiation of mouse neuroblastoma cells "

Manually curated Databases

No curated data.