Gene interactions and pathways from curated databases and text-mining
J Immunol 2008, PMID: 18490760

Differential involvement of IkappaB kinases alpha and beta in cytokine- and insulin-induced mammalian target of rapamycin activation determined by Akt.

Dan, Han C; Baldwin, Albert S

The mammalian target of rapamycin (mTOR) is a mediator of cell growth, survival, and energy metabolism at least partly through its ability to regulate mRNA translation. mTOR is activated downstream of growth factors such as insulin, cytokines such as TNF, and Akt-dependent signaling associated with oncoprotein expression. mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2), and activation of Akt induces phosphorylation of TSC2, which blocks the repressive TSC1/2 activity. Previously, we showed that activation of mTOR in PTEN-deficient cancer cells involves IkappaB kinase (IKK) alpha, a catalytic subunit of the IKK complex that controls NF-kappaB activation. Recently, a distinct IKK subunit, IKKbeta, was shown to phosphorylate TSC1 to promote mTOR activation in an Akt-independent manner in certain cells stimulated with TNF and in some cancer cells. In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF-induced mTOR activation. Insulin activation of mTOR requires Akt in a manner that involves IKKalpha, preferentially to IKKbeta, and TSC2 phosphorylation. TNF, in most cells examined, activates Akt to use IKKalpha to control mTOR activation. In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR. The results show that Akt-dependent signaling, induced by cytokines or insulin, alters the IKK subunit-dependent control of mTOR.

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Text Mining Data

mammalian target of rapamycin → insulin: " Differential involvement of IkappaB kinases alpha and beta in cytokine- and insulin induced mammalian target of rapamycin activation determined by Akt "

tuberous sclerosis complex 1/2 (TSC1/2) → Akt: " mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2) , and activation of Akt induces phosphorylation of TSC2, which blocks the repressive TSC1/2 activity "

TSC2 → tuberous sclerosis complex 1/2 (TSC1/2): " mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2) , and activation of Akt induces phosphorylation of TSC2 , which blocks the repressive TSC1/2 activity "

TSC2 → Akt: " mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2), and activation of Akt induces phosphorylation of TSC2 , which blocks the repressive TSC1/2 activity "

mTOR → Akt: " mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2), and activation of Akt induces phosphorylation of TSC2, which blocks the repressive TSC1/2 activity "

tuberous sclerosis complex 1/2 (TSC1/2) — mTOR: " mTOR is negatively controlled by the tuberous sclerosis complex 1/2 (TSC1/2) , and activation of Akt induces phosphorylation of TSC2, which blocks the repressive TSC1/2 activity "

mTOR → IkappaB kinase (IKK): " Previously, we showed that activation of mTOR in PTEN-deficient cancer cells involves IkappaB kinase (IKK) alpha, a catalytic subunit of the IKK complex that controls NF-kappaB activation "

mTOR → TNF: " In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF induced mTOR activation "

mTOR — IKKalpha: " In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF induced mTOR activation "

mTOR — IKKbeta: " In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF induced mTOR activation "

insulin- — IKKalpha: " In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF induced mTOR activation "

insulin- — IKKbeta: " In this study, we have explored the involvement of both IKKalpha and IKKbeta in insulin- and TNF induced mTOR activation "

Akt → mTOR: " Insulin activation of mTOR requires Akt in a manner that involves IKKalpha, preferentially to IKKbeta, and TSC2 phosphorylation "

Akt → Insulin: " Insulin activation of mTOR requires Akt in a manner that involves IKKalpha, preferentially to IKKbeta, and TSC2 phosphorylation "

Insulin → mTOR: " Insulin activation of mTOR requires Akt in a manner that involves IKKalpha, preferentially to IKKbeta, and TSC2 phosphorylation "

Akt → TNF: " TNF , in most cells examined, activates Akt to use IKKalpha to control mTOR activation "

Akt → TNF: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

mTOR → TNF: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

mTOR → Akt: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

mTOR → IKKbeta: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

IKKbeta → TNF: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

IKKbeta → Akt: " In MCF7 cells, TNF does not activate Akt and requires IKKbeta to activate mTOR "

Manually curated Databases

  • IRef Innatedb Interaction: CHUK — MTOR (unknown, -)
In total, 1 gene pairs are associated to this article in curated databases