Gene interactions and pathways from curated databases and text-mining
Life Sci 2009, PMID: 19303025

Caffeic acid phenethyl ester accumulates beta-catenin through GSK-3beta and participates in proliferation through mTOR in C2C12 cells.

Lee, Eun Soo; Lee, Jung-Ok; Lee, Soo Kyung; Kim, Ji Hae; Jung, Jin Hee; Keum, Bora; Park, Sun-Hwa; Kim, Hyeon Soo

OBJECTIVE

The aim of this study is to characterize the roles of caffeic acid phenethyl ester (CAPE) in the skeletal muscle cells.

METHODS

We performed immunoblotting assay using various phosphorylation specific antibodies.

RESULTS

We found that CAPE induces rapid and transient phosphorylation of glycogen synthase kinase (GSK)-3beta in a phosphoinositide 3-kinase (PI3K)-dependent manner. CAPE also decreases phosphorylation of beta-catenin, ultimately leading to beta-catenin accumulation. In addition, we demonstrated that CAPE activated the mammalian target of rapamycin (mTOR)-p70 S6 ribosomal kinase (S6K) and also stimulated extracellular signal-regulated kinase (ERK). The inhibition of mTOR blocked CAPE-induced ERK phosphorylation.

CONCLUSIONS

Our results suggest that CAPE may act through beta-catenin accumulation via stimulation of GSK-3beta and may also participate in cellular proliferation through the mTOR-ERK pathway.

Document information provided by NCBI PubMed

Text Mining Data

glycogen synthase kinase (GSK)-3beta — phosphoinositide 3-kinase (PI3K): " We found that CAPE induces rapid and transient phosphorylation of glycogen synthase kinase (GSK)-3beta in a phosphoinositide 3-kinase (PI3K) dependent manner "

ERK → mTOR: " The inhibition of mTOR blocked CAPE induced ERK phosphorylation "

Manually curated Databases

No curated data.