Gene interactions and pathways from curated databases and text-mining
Journal of clinical biochemistry and nutrition 2010, PMID: 20216949

Effect of paraquat-induced oxidative stress on insulin regulation of insulin-like growth factor-binding protein-1 gene expression.

Kimura, Kumi; Katsumata, Yoshihito; Ozawa, Takahiro; Tawara, Satoshi; Igarashi, Kiharu; Cho, Yoshitake; Shibata, Norihiro; Hakuno, Fumihiko; Takahashi, Shin-Ichiro; Takenaka, Asako

Oxidative stress is thought to play a role in the development of insulin resistance. In order to elucidate the molecular effect of oxidative stress on liver insulin signaling, we analyzed the effect of paraquat (1,1-dimethyl-4,4-dipyridynium; PQ)-derived oxidative stress on the expression of insulin-dependent genes and activation of liver insulin signaling pathway. Incubation of primary cultured rat hepatocytes with 2 mM PQ for 6 h impaired the suppressive effect of insulin on insulin-like growth factor-binding protein-1 (IGFBP-1) gene expression, but did not influence glucose-6-phosphatase gene expression. Insulin-dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment. In contrast, PQ treatment impaired insulin-dependent phosphorylation of mammalian target of rapamycin (mTOR). These results indicate that PQ-induced oxidative stress impairs insulin-dependent mTOR activation and that this impairment probably causes inhibition of insulin-dependent repression of IGFBP-1 expression.

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Text Mining Data

insulin-like growth factor — insulin: " Effect of paraquat induced oxidative stress on insulin regulation of insulin-like growth factor binding protein-1 gene expression "

forkhead in rhabdomyosarcoma → Insulin: " Insulin dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment "

Akt → Insulin: " Insulin dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment "

insulin receptor → Insulin: " Insulin dependent phosphorylation or activation of insulin receptor , insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment "

insulin receptor substrate-1 → Insulin: " Insulin dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2, phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment "

insulin receptor substrate-1 and -2 → Insulin: " Insulin dependent phosphorylation or activation of insulin receptor, insulin receptor substrate-1 and -2 , phosphatidylinositol 3-kinase, Akt and forkhead in rhabdomyosarcoma were not affected by PQ pre-treatment "

mammalian target of rapamycin (mTOR) → insulin: " In contrast, PQ treatment impaired insulin dependent phosphorylation of mammalian target of rapamycin (mTOR) "

IGFBP-1 ⊣ insulin: " These results indicate that PQ-induced oxidative stress impairs insulin dependent mTOR activation and that this impairment probably causes inhibition of insulin dependent repression of IGFBP-1 expression "

mTOR → insulin: " These results indicate that PQ-induced oxidative stress impairs insulin dependent mTOR activation and that this impairment probably causes inhibition of insulin dependent repression of IGFBP-1 expression "

Manually curated Databases

No curated data.