Cancer prevention research (Philadelphia, Pa.) 2010,
Chen, Wenxing; Luo, Yan; Liu, Lei; Zhou, Hongyu; Xu, Baoshan; Han, Xiuzhen; Shen, Tao; Liu, Zhijun; Lu, Yin; Huang, Shile
Cryptotanshinone (CPT), a natural compound isolated from the plant Salvia miltiorrhiza Bunge, is a potential anticancer agent. However, little is known about its anticancer mechanism. Here, we show that CPT inhibited cancer cell proliferation by arresting cells in G(1)-G(0) phase of the cell cycle. This is associated with the inhibition of cyclin D1 expression and retinoblastoma (Rb) protein phosphorylation. Furthermore, we found that CPT inhibited the signaling pathway of the mammalian target of rapamycin (mTOR), a central regulator of cell proliferation. This is evidenced by the findings that CPT inhibited type I insulin-like growth factor I- or 10% fetal bovine serum-stimulated phosphorylation of mTOR, p70 S6 kinase 1, and eukaryotic initiation factor 4E binding protein 1 in a concentration- and time-dependent manner. Expression of constitutively active mTOR conferred resistance to CPT inhibition of cyclin D1 expression and Rb phosphorylation, as well as cell growth. The results suggest that CPT is a novel antiproliferative agent.
Diseases/Pathways annotated by Medline MESH:
Document information provided by NCBI PubMed
Text Mining Data
cyclin D1 → Mammalian target of rapamycin: " Cryptotanshinone inhibits cancer cell proliferation by suppressing Mammalian target of rapamycin
mediated cyclin D1
expression and Rb phosphorylation "
Manually curated Databases
No curated data.