Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2013, PMID: 24036451

PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription.

Kamano, Yuya; Saeki, Makio; Egusa, Hiroshi; Kakihara, Yoshito; Houry, Walid A; Yatani, Hirofumi; Kamisaki, Yoshinori

PIH1D1 is the defining component of the R2TP complex. Recently, R2TP has been reported to stabilize mTOR (mammalian target of rapamycin), an important regulator of cell growth and protein synthesis. Two complexes of mTOR, mTORC1 and mTORC2, have been identified. We demonstrate that immunoprecipitation (IP) of PIH1D1 results in the co-IP of Raptor (mTORC1 specific), but not Rictor (mTORC2 specific), and that knockdown of PIH1D1 decreases mTORC1 assembly, S6 kinase phosphorylation (indicator of mTORC1 activity), and rRNA transcription without affecting mTORC2 in human breast cancer MCF-7 cells. In addition, we provide evidence that PIH1D1 is overexpressed in various breast cancer cell lines. These findings collectively suggest that PIH1D1 may have an important role in mTORC1 regulation in breast cancers.

Diseases/Pathways annotated by Medline MESH: Breast Neoplasms
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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Intact Interaction: Complex of PIH1D1-MTOR-RPTOR (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of ETV7-RPTOR-PIH1D1-MTOR (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of RPTOR-PIH1D1 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of MTOR-ETV7-RICTOR (association, anti bait coimmunoprecipitation)
In total, 8 gene pairs are associated to this article in curated databases