Gene interactions and pathways from curated databases and text-mining
Cancer Res 1998, PMID: 9731515

Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.

Akagi, Y; Liu, W; Zebrowski, B; Xie, K; Ellis, L M

We investigated the role of insulin-like growth factor (IGF)-I and IGF-binding proteins (IGFBPs) in the regulation of vascular endothelial growth factor (VEGF) expression in colon cancer cells and the mechanism by which this regulation occurs. HT29 human colon cancer cells were treated with IGF-I for various time periods. VEGF mRNA expression increased within 2 h and peaked at 24 h. SW620 colon cancer cells exhibited a peak induction of VEGF mRNA 8 h after IGF-I treatment. IGF-I induction of VEGF was confirmed at the protein level. In experiments using transient transfection of VEGF promoter-reporter constructs into HT29 cells, IGF-I increased the activity of the VEGF promoter, and pretreatment of HT29 cells with dactinomycin abrogated the induction of VEGF mRNA by IGF-I. The half-life of VEGF mRNA was not prolonged by treatment with IGF-I. Blocking the activity of IGFBP-4 did not significantly modulate the effect of IGF-I induction of VEGF mRNA in HT29 cells. Treating cells with des-(1-3)-IGF-I (an active derivative of IGF-I that does not bind to binding proteins) had effects on VEGF mRNA expression that were similar to those of IGF-I. These findings suggest that IGF-I regulates VEGF expression in human colon cancer cells by induction of transcription of the VEGF gene. IGFBPs do not significantly affect IGF-I induction of VEGF.

Diseases/Pathways annotated by Medline MESH: Colonic Neoplasms
Document information provided by NCBI PubMed

Text Mining Data

vascular endothelial growth factor — insulin-like growth factor-I: " Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I "

vascular endothelial growth factor ⊣ (IGF)-I: " We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs "

vascular endothelial growth factor ⊣ IGF binding proteins ( IGFBPs ): " We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs "

vascular endothelial growth factor ⊣ insulin-like growth factor (IGF)-I: " We investigated the role of insulin-like growth factor (IGF)-I and IGF binding proteins ( IGFBPs ) in the regulation of vascular endothelial growth factor ( VEGF ) expression in colon cancer cells and the mechanism by which this regulation occurs "

VEGF → IGF-I: " IGF-I induction of VEGF was confirmed at the protein level "

VEGF → IGF-I: " In experiments using transient transfection of VEGF promoter-reporter constructs into HT29 cells, IGF-I increased the activity of the VEGF promoter, and pretreatment of HT29 cells with dactinomycin abrogated the induction of VEGF mRNA by IGF-I "

VEGF → IGF-I: " These findings suggest that IGF-I regulates VEGF expression in human colon cancer cells by induction of transcription of the VEGF gene "

VEGF → IGF-I: " IGFBPs do not significantly affect IGF-I induction of VEGF "

Manually curated Databases

No curated data.