◀ Back to INS
INS — MAPK7
Text-mined interactions from Literome
Noguchi et al., EMBO J 1999
:
Overexpression of wild-type Dok, but not that of DokDeltaPH or DokY361F, enhanced the cell migratory response to insulin without affecting
insulin activation of
mitogen activated protein kinase
de la Monte et al., Alcohol Clin Exp Res 1999
:
This study explores the roles of the Grb2- and Syp binding motifs of IRS-1 in relation to the inhibitory effects of ethanol on
insulin stimulated DNA synthesis, proliferating cell nuclear antigen ( PCNA ) and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) expression, and activation of mitogen activated protein kinase (
MAPK ), which is known to be essential for cell proliferation ... Ethanol exposure decreased
insulin stimulated DNA synthesis, PCNA, GAPDH, and activated
MAPK levels in all clones, but the wild-type IRS-1 transfectants were relatively resistant, and the IRS-1deltaGrb2 transfectants were extraordinarily sensitive to these inhibitory effects of ethanol
Hsueh et al., Am J Cardiol 1999
(Insulin Resistance) :
MAPK may
mediate the effects of
insulin and angiotensin II on VSMC production of plasminogen activator inhibitor-1, which attenuates fibrinolysis
Yoshimata et al., Life Sci 1999
:
Platelet derived growth factor-BB ( PDGF-BB ), epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF), but not
insulin-like growth factor-1 (IGF-1) ,
stimulated MAPK activity
Fischer et al., Toxicol Appl Pharmacol 1999
:
However, inhibition of
MAPK activation by a specific inhibitor, PD-98059 ( 10.0 microM ) did not
prevent insulin release induced by PCBs
She et al., FEBS Lett 1999
:
Zinc, but not EtOH, also promoted the stimulatory
effects of
insulin on activating phosphorylation of p42/p44
mitogen activated protein ( MAP ) kinases
Yau et al., Eur J Biochem 1999
(Liver Neoplasms, Experimental) :
In contrast with these results, both
insulin and IGF-I
induced mitogen activated protein ( MAP ) kinase phosphorylation and activity at a concentration of 10 nM
Uchida et al., Mol Cell Biol 2000
:
Insulin promoted the association of Grb-2 with IRS-1 and IRS-4, whereas IRS-2 weakly bound Grb-2 ; consequently, IRS-1 and IRS-4 enhanced
insulin stimulated
mitogen activated protein kinase activity
Gingras et al., Mol Endocrinol 2000
(Breast Neoplasms) :
Furthermore, it has been suggested that the IRS proteins are part of the signaling complexes that lead to
activation of the
mitogen activated protein ( MAP ) kinase by
insulin ; thus we investigated the potential role of the MAP kinase ( MAPK ) cascade in the IL-4 action ... Wortmannin also blocked
MAPK activation by IL-4, IGF-I, and
insulin , suggesting that the MAPK cascade acts as a downstream effector of PI 3-kinases
Knebel et al., Biochem Biophys Res Commun 2000
(Insulin Resistance) :
MAPK phosphorylation and activity were unaltered, but inducibility of cfos transcription was strongly
impaired by
insulin and reduced by PDGF
Chung et al., Cell Signal 2000
:
In serum starved CK expressor cells,
insulin and IGF-I
stimulated DNA synthesis, p70 S6 kinase (p70 S6K) activity, phosphatidylinositol 3-kinase (PI3K) activity, and activating phosphorylation of p42/p44 mitogen activated protein kinases (
MAPK ) to greater extents than in the corresponding vector control cells
Weng et al., Eur J Pharmacol 2000
:
Platelet activating factor (PAF), tumor necrosis factor-alpha (TNF-alpha), and
insulin-like growth factor-1 (IGF-1) had little effect on
MAPK activation
Takehara et al., Biochim Biophys Acta 2000
:
In conclusion, high glucose enhances insulin induced mitogenesis associated with the potentiation of
insulin stimulated
MAPK activity in VSMC
Liu et al., Biochem Biophys Res Commun 2000
(MAP Kinase Signaling System) :
Insulin activation of
mitogen activated protein ( MAP ) kinase and Akt is phosphatidylinositol 3-kinase dependent in rat adipocytes
Formisano et al., Mol Cell Biol 2000
(MAP Kinase Signaling System) :
In contrast, blocking c-Ras with lovastatin or the use of the L61,S186 dominant negative Ras mutant inhibited
insulin stimulated
MAPK activity and DNA synthesis by only about 30 % but completely blocked the effect of EGF
Igarashi et al., Eur J Clin Invest 2000
(MAP Kinase Signaling System) :
Insulin activates p38
mitogen activated protein ( MAP ) kinase via a MAP kinase kinase (MKK) 3/MKK 6 pathway in vascular smooth muscle cells ... In this study, we investigated the
effect of
insulin on p38
mitogen activated protein ( MAP ) kinase activation in cultured rat vascular smooth muscle cells ( VSMC )
Jensen et al., J Biol Chem 2000
:
Subsequent basal and
insulin induced phosphorylation of the insulin receptor, IRS-1,
MAPK , Akt kinase, and GSK-3 were similar in control and pretreated cells
Farhang-Fallah et al., J Biol Chem 2000
:
Furthermore, expression of this dominant negative PHIP mutant leads to a marked attenuation of
insulin stimulated
mitogen activated protein kinase activity
Kawabe et al., J Hypertens 2000
(Insulin Resistance) :
Assays for
insulin induced activities of insulin receptor substrate (IRS)-1, phosphoinositide 3-kinase (PI3-K) and
mitogen activated protein kinase ( MAPK ) were performed ...
Insulin stimulated activities of IRS-1 and PI3-K, but not
MAPK activity, were also attenuated in the presence of high concentrations of glucose
Kayali et al., Diabetes 2000
:
Phosphorylation of p38
MAPK was
stimulated strongly by sorbitol ( 22-fold ) but weakly by
insulin ( 2.7-fold ) ... To determine the functional significance of the observed activation of p38
MAPK in
response to
insulin and osmotic shock, we used three pyridinyl imidazole p38 MAPK inhibitors, SB203580, SB202190, and PD169316
Osman et al., J Appl Physiol 2001
(Insulin Resistance...) :
Seven weeks of training significantly increased basal and
insulin stimulated ERK2 and RSK2 activities, as well as insulin stimulation of
MAPK kinase activity
Carrillo et al., Endocrinology 2001
:
However, this PI 3-K inhibitor also reduced
insulin mediated phosphorylation of p44/p42
MAPK in cultured rat hepatocytes, indicating that both the PI 3-K and MAPK pathways could be involved in PK-L activation by insulin
Kemp et al., Endocrinology 2001
:
Insulinotropic hormone glucagon-like peptide 1 (GLP-1) activation of
insulin gene promoter
inhibited by p38
mitogen activated protein kinase
Robinson et al., Eur J Pharmacol 2001
:
For comparison,
insulin mediated increases in p42/p44
MAPK were blocked by genistein and tyrphostin A47
Weng et al., Hum Mol Genet 2001
(Breast Neoplasms) :
PTEN inhibits
insulin stimulated
MEK/MAPK activation and cell growth by blocking IRS-1 phosphorylation and IRS-1/Grb-2/Sos complex formation in a breast cancer model ... Our data suggest that PTEN blocks
MAPK phosphorylation in
response to
insulin stimulation by inhibiting the phosphorylation of IRS-1 and IRS-1/Grb2/Sos complex formation, which leads to downregulation of cyclin D1, inhibition of cell cycle progression and suppression of cell growth
Tortorella et al., J Biol Chem 2001
:
In C2C12 myoblast cells,
insulin-like growth factor-1 and basic fibroblast growth factor (bFGF)
activate MAPK/Erk , and both growth factors promote myoblast proliferation
Fujishiro et al., J Biol Chem 2001
:
p38 mitogen activated protein kinase (
MAPK ), which is situated downstream of MAPK kinase ( MKK ) 6 and MKK3, is
activated by mitogenic or stress inducing stimuli, as well as by
insulin
Gasparini et al., J Neurosci 2001
(Alzheimer Disease...) :
Stimulation of beta-amyloid precursor protein trafficking by
insulin reduces intraneuronal beta-amyloid and
requires mitogen activated protein kinase signaling
Byon et al., Mol Cell Biochem 2001
:
In conclusion, induction of MKP-1 mRNA expression in Hirc B cells by
insulin requires activation of both the ERK and JNK pathways, but not
p38MAPK
Canesi et al., Gen Comp Endocrinol 2001
:
Moreover, both zinc and insulin induced activation of mitogen activated protein kinases ( MAPKs ) ; however, whereas zinc gave a clear effect on the stress activated p-38 MAPK,
insulin activated extracellular activated
MAPK ( ERK2 ) and inhibited p-38
Blero et al., Biochem Biophys Res Commun 2001
:
Overexpression of SHIP2 led to a decrease of the
insulin dependent PIP3 production as well as Akt/PKB activation and
MAPK stimulation
Indolfi et al., Circulation 2001
(Carotid Artery Diseases...) :
Furthermore, in VSMC culture,
insulin increased
MAPK activation
Ranginwale et al., Exp Cell Res 2001
(MAP Kinase Signaling System) :
In contrast, insulin activates only low levels of JNK-P, and, surprisingly, we find that
insulin induces only low levels of
MAPK-P , indicating that insulin and activated normal p21 utilize MAP kinase independent signal transduction pathways
Nikoulina et al., J Clin Endocrinol Metab 2001
(Diabetes Mellitus, Type 2) :
Protein expression of IRS-1, p85, p110, Akt, p70 S6 kinase, and MAPK were normal in diabetic cells, as was
insulin stimulated phosphorylation of Akt, p70 S6 kinase, and
MAPK
Wick et al., J Biol Chem 2001
(MAP Kinase Signaling System) :
Mutations at Tyr ( 362/398 ) of p62(dok) disrupted the interaction between p62(dok) and GAP and decreased the inhibitory effect of p62(dok) on the
insulin stimulated activation of Ras and Akt, but not
mitogen activated protein kinase
Somwar et al., J Biol Chem 2001
:
In contrast,
insulin dependent p38
MAPK phosphorylation was efficiently reduced in cells pretreated with wortmannin, with an IC ( 50 ) of 7 nm ... We propose that a distinct, high affinity target of wortmannin, other than PI 3-kinase, may be necessary for activation of p38
MAPK and GLUT4 in
response to
insulin
Somwar et al., Biochem J 2001
(MAP Kinase Signaling System) :
Insulin caused a dose- ( EC ( 50 ) =15 nM ) and time- ( t ( 1/2 ) =3 min ) dependent increase in p38
MAPK phosphorylation, which peaked at 10 min ( 2.3+/-0.3-fold )
Mehrhof et al., Circulation 2001
:
In cardiomyocyte hypoxia,
insulin-like growth factor-I induced antiapoptotic signaling
requires phosphatidylinositol-3-OH-kinase dependent and
mitogen activated protein kinase dependent activation of the transcription factor cAMP response element binding protein
Booth et al., J Biol Chem 2002
(MAP Kinase Signaling System) :
Several recent studies have demonstrated that
insulin-like growth factor (IGF)-1 induced
mitogen activated protein kinase ( MAP kinase ) activation is abolished by pertussis toxin, suggesting that trimeric G proteins of the G ( i ) class are novel cellular targets of the IGF-1 signaling pathway
Gosmanov et al., Diabetes 2002
:
PI 3-kinase inhibitors also abolished
insulin stimulated p38
MAPK phosphorylation in the plantaris muscle and Akt phosphorylation in both muscles
Wang et al., Sheng Li Xue Bao 2002
:
In order to study the
effect of
insulin on the intracellular signal transduction (
PKC-MAPK ) probably involved in the modulation of proliferation and redistribution of actins in the VSMCs, the DNA synthesis, MAPK activities and its gene expression, and the redistribution of intracellular actins were investigated in the isolated VSMCs of SHR pretreated with PKC inhibitor and/or insulin, respectively
Yamada et al., Int J Exp Diabetes Res 2002
(MAP Kinase Signaling System) :
However, the presence of
insulin enhanced both DNA synthesis and
MAPK activation by PDGF
Molero et al., Mol Cell Endocrinol 2002
(MAP Kinase Signaling System) :
Here we analysed the
stimulation of
MAPK by
insulin and vanadate and observed that both elicit a rapid 3.5-4 fold activation which is abolished by wortmannin and PD98059
Berg et al., Biochem Biophys Res Commun 2002
(Insulin Resistance) :
In contrast, rapamycin had no effect on the decrease in
insulin induced
MAPK phosphorylation or GLUT-4 protein levels
Kaburagi et al., Endocr J 1999
(Insulin Resistance) :
To study the roles of these IRSs in mediating insulin actions, we analyzed liver, muscle and adipocytes, the major targets of insulin actions, from IRS-1 null mice which we previously generated, and showed that : 1 )
insulin stimulated activation of PI 3-kinase,
mitogen activated protein kinase and glucose transport were impaired in muscles from IRS-1 null mice which was in contrast to the grossly normal signaling and actions in livers from these mice ; 2 ) the difference in the degree of insulin resistance in these two major insulin targets appeared to depend on the amount of tyrosine phosphorylation of IRS-2 compensating for IRS-1 deficiency ; 3 ) insulin induced activation of PI 3-kinase, glucose transport and GLUT4 translocation were impaired but not abolished in adipocytes from these mice in which IRS-3 was the major tyrosine phosphorylated protein activating PI 3-kinase and at least partially mediating some residual insulin actions in the absence of IRS-1
Jacob et al., Am J Physiol Cell Physiol 2002
:
Insulin also
caused a 60 % decrease in PDGF stimulated
mitogen activated protein kinase ( MAPK ) phosphorylation and activation ...
Insulin inhibition of
MAPK was accompanied by a rapid induction of MAPK phosphatase ( MKP-1 ), which inactivates MAPKs by dephosphorylation
Kumar et al., Br J Pharmacol 2002
(Insulin Resistance) :
6 All the three mitogen activated protein kinases (
MAPK ) were
activated by
insulin in sensitive myotubes
Galbiati et al., Endocrinology 2002
(Insulinoma...) :
MAPK kinase inhibitor ( UO126 ) and staurosporine reduced calcitriol mediated betaTC ( 3 ) death, and down-regulation of
insulin gene transcription was
prevented by staurosporine but not UO126
Gosmanov et al., Am J Physiol Regul Integr Comp Physiol 2003
:
Glucose, but not mannitol, impaired
insulin stimulated phosphorylation of Akt and p38
MAPK in the plantaris and soleus muscles, respectively
Shimizu et al., Endocrinology 2002
:
Overexpression of a wild-type PTP1B in L1 adipocytes as well as in L6 myocytes, led to a profound decrease in
insulin stimulated phosphorylation of
MAPK
Miakotina et al., Respir Res 2002
:
Insulin did not
affect p44/42
mitogen activated protein kinase ( MAPK ) phosphorylation and the insulin inhibition of SP-A mRNA levels was not affected by PD98059, an inhibitor of the p44/42 MAPK pathway
Pirola et al., J Biol Chem 2003
(MAP Kinase Signaling System) :
IRS-2 appears to be the chief molecule responsible for
MAPK and PKB
activation by
insulin , as knockdown of IRS-2 ( but not IRS-1 ) by RNA interference severely impaired activation of both kinases
Thong et al., J Appl Physiol 2003
:
Insulin induced a modest sustained 1.2- and 1.3-fold increase ( P < 0.05 ) in p38
MAPK phosphorylation in the rested and exercised legs, respectively
Hyakukoku et al., Hypertens Res 2003
(Insulin Resistance...) :
Insulin induced tyrosine phosphorylation of insulin receptor beta subunit (IRbeta) and insulin receptor substrate-1 (IRS-1) and tyrosine/threonine phosphorylation of p44/42
MAPK ( ERK-1/2 ) were evaluated
He et al., J Biol Chem 2003
(MAP Kinase Signaling System) :
Insulin dependent translocation of Shc, SOS1, and
MAPK to lipid raft microdomains was markedly attenuated by FLNa expression ... Ectopic expression of a C-terminal fragment of FLNa ( FLNaCT ) in HepG2 cells blocked the endogenous IR-FLNa interaction and potentiated
insulin stimulated
MAPK phosphorylation and transactivation of Elk-1 compared with vector transfected cells ... We report here that human melanoma M2 cells lacking FLNa expression exhibited normal insulin receptor (IR) signaling, whereas FLNa expressing A7 cells were unable to elicit
insulin dependent Shc tyrosine phosphorylation and p42/44
MAPK activation despite no significant defect in IR-stimulated phosphorylation of insulin receptor substrate-1 or activation of the phosphatidylinositol 3-kinase/AKT cascade
Seto-Young et al., J Clin Endocrinol Metab 2003
(MAP Kinase Signaling System) :
The
role of
mitogen activated protein kinase in
insulin and insulin-like growth factor I (IGF-I) signaling cascades for progesterone and IGF binding protein-1 production in human granulosa cells ...
MAPK was
stimulated by
insulin up to 350 % of the baseline value
Sakaue et al., J Biol Chem 2003
(MAP Kinase Signaling System) :
The phosphorylation both of Akt on serine 473 and of p42 and p44 isoforms of
mitogen activated protein kinase induced by
insulin was not affected by Cre expression, indicating that the latter specifically inhibits PDK-1 dependent signaling
Koistinen et al., Diabetologia 2003
(Diabetes Mellitus, Type 2) :
In vitro
effects of
insulin ( 120 nmol/l ) or AICAR ( 1 mmol/l ) on p38
MAPK expression and phosphorylation were determined in skeletal muscle from non-diabetic ( n=6 ) and Type 2 diabetic ( n=9 ) subjects ...
Insulin increases p38
MAPK phosphorylation in skeletal muscle from non-diabetic subjects, but not in Type 2 diabetic patients
Tajmir et al., J Cell Physiol 2003
:
Insulin stimulated phosphorylation of Akt on S473 or of p38
MAPK were unaffected
Kwon et al., J Cell Biol 2003
:
Expression of the PH-EF construct blocked endogenous PLCgamma1 binding to the IR and inhibited
insulin dependent phosphorylation of
mitogen activated protein kinase ( MAPK ), but not AKT ... Silencing PLCgamma1 expression using small interfering RNA markedly reduced
insulin dependent
MAPK regulation in HepG2 cells ... Our results show that PLCgamma1 is a thiol-reactive protein whose association with the IR could contribute to the
activation of
MAPK signaling by
insulin
Langlais et al., Mol Endocrinol 2004
:
Negative regulation of
insulin stimulated
mitogen activated protein kinase signaling by Grb10 ... To determine the mechanism by which Grb10 inhibited
insulin stimulated
MAPK signaling, we examined whether Grb10 affects the phosphorylation of MAPK upstream signaling components
PĆ©rez et al., Diabetes 2004
:
However, at elevated concentrations, leptin impaired
insulin stimulated
MAPK activity, glycogen synthase kinase (GSK)3beta phosphorylation, and insulin receptor tyrosine phosphorylation without altering vanadate stimulation ... Central administration of leptin decreased
insulin effects on adipocyte
MAPK and GSK3beta phosphorylation ... In insulin-resistant aged rats with hyperleptinemia and central leptin resistance,
insulin poorly
stimulated MAPK and central leptin infusion did not further deteriorate adipocyte insulin responsiveness ... Food restriction increased
MAPK stimulation by
insulin and restored the ability of centrally infused leptin to attenuate adipocyte insulin signaling in aged rats
de Alvaro et al., J Biol Chem 2004
(Insulin Resistance) :
Moreover, TNF-alpha produced inhibitor kappaB kinase (IKK)-beta activation and inhibitor kappaB-beta and -alpha degradation in a p38
MAPK dependent manner, and treatment with salicylate ( an inhibitor of IKK ) completely restored
insulin signaling
Schliess et al., J Biol Chem 2004
:
Insulin increases [ Tyr ( P ) ( 418 ) ] Src, [ Tyr ( P ) ( 397 ) ] FAK, and dual p38 (
MAPK ) phosphorylation by about 2-fold
Perdomo et al., J Biol Chem 2004
(Insulin Resistance) :
Two to 3-fold overexpression of L-CPT I, the endogenous isoform in L6 cells, proportionally increased oxidation of the long-chain fatty acids palmitate and oleate and increased insulin stimulation of [ ( 14 ) C ] glucose incorporation into glycogen by 60 % while enhancing
insulin stimulated phosphorylation of
p38MAPK
Li et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003
:
High glucose, AGE, high
insulin and H ( 2 ) O ( 2 ) could
activate P38MAPK by oneself, and increased notably expression of phosphorylated-P38MAPK and P-selectin on cell line ECV304 ; Expression of P-selectin was inhibited significantly by SB203580
Leng et al., Diabetes 2004
(MAP Kinase Signaling System...) :
Effects of
insulin , contraction, and phorbol esters on
mitogen activated protein kinase signaling in skeletal muscle from lean and ob/ob mice ...
Insulin increased phosphorylation of JNK, p38
MAPK , and ERK1/2 in isolated extensor digitorum longus (EDL) and soleus muscle from lean mice in a time- and dose dependent manner ...
Insulin action on JNK, p38
MAPK , and ERK1/2 phosphorylation was significantly impaired in EDL and soleus muscle from ob/ob mice ... In conclusion,
insulin , contraction, and PMA
activate MAPK signaling in skeletal muscle
Le et al., J Ethnopharmacol 2004
(Hyperlipidemias) :
In vivo Nigella sativa treatment resulted in greater dose dependent activation of
MAPK and PKB in
response to
insulin
Jacob et al., Am J Physiol Cell Physiol 2004
(Diabetes Mellitus, Type 2...) :
In normal WKY VSMC,
insulin increased MAPK phosphatase-1 (MKP-1) expression as well as MKP-1 phosphorylation, which stabilizes it, and inhibited PDGF mediated
MAPK phosphorylation and cell migration
RibƩ et al., Endocrinology 2005
:
In contrast to data obtained in muscle and adipocyte cell lines, we found that no
insulin activation of p38
MAPK occurred in rat adipose cells
Hers et al., Biochem J 2005
:
The effect of
insulin on phosphorylation of both these sites
required the activation of PI3K and the MAPKs ( mitogen activated protein kinases ) ERK1/2 ( extracellular-signal regulated kinase 1 and 2 ), but not the activation of mTOR ( mammalian target of rapamycin ) /p70S6 kinase, JNK ( c-Jun N-terminal kinase ) or
p38MAPK
Nihei et al., Clin Exp Pharmacol Physiol 2005
:
In the
presence of either SB203580 or
insulin , activation of p38
MAPK was significantly inhibited compared with stimulation by oxLDL alone ... It was also demonstrated that
insulin inhibited oxLDL induced apoptosis and may
inhibit the activation of p38
MAPK through the PI3K/Akt pathway
Han et al., Life Sci 2005
:
Insulin significantly
increased the phosphorylation of p38
MAPK but not p44/42 MAPKs
Grohmann et al., Exp Cell Res 2005
(MAP Kinase Signaling System...) :
Insulin caused a dose dependent increase in GSK-3 phosphorylation and an increase in
MAPK phosphorylation over time, with increased sensitivity in subcutaneous preadipocytes
Ando et al., J Biol Chem 1992
:
Enhanced
insulin induced mitogenesis and
mitogen activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine
Gosmanov et al., Am J Physiol Endocrinol Metab 2006
(Hyperglycemia) :
Furthermore, incubation of HAEC with 30 mM glucose resulted in a new
insulin stimulated extracellular signal regulated kinase-1/2
mitogen activated protein kinase phosphorylation and increased lipid peroxidation and production of reactive oxygen species
Freude et al., Diabetes 2005
(Diabetes Mellitus, Type 2...) :
Peripheral
insulin stimulation rapidly
increased insulin receptor tyrosine phosphorylation,
mitogen activated protein kinase and phosphatidylinositol (PI) 3-kinase pathway activation, and dose dependent tau phosphorylation at Ser202 in the central nervous system
Zhang et al., Biochem J 2006
:
Resveratrol effectively inhibits
insulin induced Akt and
MAPK ( mitogen activated protein kinase ) activation mainly through disruption of the interactions between insulin receptor substrates and its downstream binding proteins including p85 regulatory subunit of phosphoinositide 3-kinase and Grb2 ( growth factor receptor bound protein 2 )
Lee et al., Diabetes Obes Metab 2006
:
In addition, we demonstrate that PI-3 kinase and ERK1/2
MAPK are
activated by
insulin in GMCs
Brautigan et al., Biochem Biophys Res Commun 2006
:
Phosphorylation of
MAPK or 4E-BP1 as markers for insulin signaling is
stimulated by vanadate plus
insulin , and chromium does not enhance the effects
Su et al., J Biol Chem 2006
:
Small interference RNA against a single Rab5 isoform had no effect on protein kinase B (PKB)/Akt or
MAPK activation by
insulin in NIH3T3 cells overexpressing human insulin receptor
Carnesecchi et al., Exp Cell Res 2006
:
We demonstrate that in control cells
insulin stimulation
leads to H2O2 generation, a biphasic activation of p42/44
MAPK and the induction of both Sp1 and HIF-1alpha
Pelletier et al., Mol Cell Biochem 2007
:
Although
insulin did not significantly
stimulate p38MAPK phosphorylation, vanadate increased it by 3.8-fold
Gray et al., J Endocrinol 2006
:
CaR activation was also associated with an activation of p42/44 mitogen activated protein kinases ( MAPK ), and CaR induced
insulin secretion was
reduced by an inhibitor of p42/44
MAPK activation
Szewczyk et al., EMBO J 2007
(Muscular Atrophy) :
In striated muscles of Caenorhabditis elegans, reduction of
insulin-like signaling via DAF-2 insulin/IGF receptor or its intramuscular effector PtdIns-3-kinase (PI3K)
causes unexpected activation of
MAP kinase ( MAPK ) , consequent activation of pre existing systems for protein degradation, and progressive impairment of mobility
He et al., Sheng Li Xue Bao 2007
:
The results showed that only p38
MAPK , but not JNK was
activated by GSA and
insulin co-incubation
Nguyen et al., Reproduction 2007
:
IGF-I and
insulin activate
mitogen activated protein kinase via the type 1 IGF receptor in mouse embryonic stem cells
Boonsong et al., Biochem Soc Trans 2007
:
Neither exercise nor
insulin affected STAT3 ( signal transducer and activator of transcription 3 ) or p38
MAPK ( mitogen activated protein kinase ) phosphorylation
Chenal et al., Eur J Neurosci 2008
:
Investigation of the putative signalling pathways leading to translation activation revealed that
insulin and IGF-1
induced p44- and p42
MAPK , Akt and mTOR phosphorylation
PĆ©rez et al., Clin Exp Pharmacol Physiol 2008
(Diabetes Mellitus...) :
Stimulation of adipocyte
MAPK activity by
insulin was higher in rats treated with both doses of acarbose, whereas higher stimulation of Akt phosphorylation was observed with the highest dose of acarbose
Foutz et al., American journal of physiology. Renal physiology 2008
(Diabetes Mellitus, Type 2...) :
Insulin increases the activity of mesangial BK channels through
MAPK signaling
Anilkumar et al., Arterioscler Thromb Vasc Biol 2008
:
After angiotensin II or TNFalpha treatment, JNK activation was augmented in Nox2 but not Nox4 transfected cells, whereas
insulin augmented phosphorylation of
p38MAPK , Akt, and GSK3beta specifically in Nox4 overexpressing cells and JNK specifically in Nox2 overexpressing cells
Zare et al., J Leukoc Biol 2008
:
In contrast, Plg production did not require NF-kappaB, was only partly
down-regulated by p38
MAPK inhibitor, and was efficiently inhibited by
insulin , indicating a different mechanism for its induction
Schulman et al., Curr Hypertens Rep 2009
(Cardiovascular Diseases...) :
In contrast,
insulin activation of the
mitogen activated protein kinase ( MAPK ) leads to vasoconstriction and pathologic vascular cellular growth
Wang et al., J Nutr Biochem 2009
(Insulin Resistance) :
Interestingly, CrPic was able to increase the basal and
insulin stimulated levels of p38
MAPK activation in the control and insulin-resistant cells
Palaniappan et al., Biol Reprod 2009
:
Inhibition of the phosphatidylinositol 3-kinase pathway did not block the insulin induced increase in SREBF1a, whereas
mitogen activated protein kinase inhibition
reduced the
insulin response
Fischer et al., Glia 2009
(MAP Kinase Signaling System) :
Accumulations of pERK1/2, p38
MAPK , pCREB, cFos and Egr1 in
response to
insulin or FGF2 were confined to MĆ¼ller glia, whereas retinal neurons did not seem to respond to growth factors
MartĆnez de Ubago et al., Biochim Biophys Acta 2009
(Carcinoma, Hepatocellular...) :
Activation of PPAR-alpha by the pharmacological agonist WY14643 in HTC hepatoma cells is sufficient to
inhibit insulin signalling and this effect is also dependent on p38
MAPK but not JNK kinase
Ketsawatsomkron et al., Vascul Pharmacol 2010
(Insulin Resistance) :
Activation of p42/p44
MAPK ( a mitogenic pathway )
induced by
insulin was further enhanced by Ang II
Oleksiewicz et al., J Appl Toxicol 2011
:
By western blotting with phospho-specific antibodies,
insulin induced phosphorylation of IRS-1, Akt, p70S6K, S6 ribosomal protein, 4E-BP1, FoxO3a, FoxO1, p44/42
MAPK and the EGFR
Seto-Young et al., Horm Metab Res 2011
(MAP Kinase Signaling System) :
Differential
roles of MAPK-Erk1/2 and
MAPK-p38 in
insulin or insulin-like growth factor-I (IGF-I) signaling pathways for progesterone production in human ovarian cells
Mulvihill et al., Diabetes 2011
(Atherosclerosis...) :
In HepG2 cells,
activation of
mitogen activated protein kinase-extracellular signal related kinase signaling by nobiletin or
insulin increased LDLR and decreased MTP and DGAT1/2 mRNA, resulting in marked inhibition of apoB100 secretion
Rondini et al., Mol Carcinog 2011
(Colorectal Neoplasms...) :
In vitro, leptin and adiponectin had no effect, whereas
insulin induced MC38 cell proliferation and
MAPK activation
Hitomi et al., Am J Hypertens 2011
(Hypertrophy...) :
To clarify the role of angiotensin II (Ang II) in insulin induced arteriosclerosis, we examined the effects of Ang II on
insulin induced
mitogen activated protein ( MAP ) kinase activation and cellular hypertrophy in rat vascular smooth muscle cells ( VSMCs )
Yuan et al., PloS one 2011
(MAP Kinase Signaling System) :
Insulin induced the activation of extracellular signal regulated kinase ( ERK ) 1/2,
mitogen activated protein kinase ( MAPK ) and RAC-alpha serine/threonine-protein kinase ( Akt )
Abdo et al., Diabetologia 2013
:
In vitro, insulin inhibited Agt but stimulated Hnrnpf and Hnrnpk expression in high-glucose media via p44/42
mitogen activated protein kinase signalling in RPTCs. Transfection with Hnrnpf or Hnrnpk small interfering RNAs
prevented insulin inhibition of Agt expression in RPTCs
PĆ©rez-PĆ©rez et al., Biol Reprod 2013
(MAP Kinase Signaling System) :
Maximal effect was achieved at 10 nM
insulin , and this effect can be totally
prevented both by blocking phosphatidylinositol 3 kinase (PI3K) pathways and
mitogen activated protein kinase ( MAPK )
Seger et al., J Biol Chem 1995
:
Differential
activation of
mitogen activated protein kinase and S6 kinase signaling pathways by 12-O-tetradecanoylphorbol-13-acetate ( TPA ) and
insulin ... Under these conditions, AG-18 inhibited insulin stimulated phosphorylation of the ribosomal protein S6, while no inhibition of
insulin induced activation of mitogen activated protein kinase ( MAPK ) kinase or
MAPK was detected
van den Berghe et al., Mol Cell Biol 1994
:
Since
mitogen activated protein ( MAP ) kinase is
activated by
insulin through phosphorylation on both tyrosine and threonine residues, we investigated whether MAP kinase and its upstream regulator, p21ras, are involved in insulin mediated glucose transport
Srinivasan et al., J Biol Chem 1994
:
Insulin and TPA also
stimulated MAPK ( > 2-fold increase over basal, with myelin basic protein as a substrate ) ... ML-9, a myosin light chain kinase inhibitor, blocked the
effects of
insulin and TPA on both
MAPK and PP-1 activation ... In these cells subsequent
effects of
insulin on
MAPK and PP-1 activation were blocked, without an effect on basal enzyme levels
Taha et al., J Biol Chem 1995
:
Insulin binding results in rapid phosphorylation of insulin receptor substrate-1 to
activate p21ras and
mitogen activated protein kinase
Corps et al., FEBS Lett 1995
:
Incubation with wortmannin ( WM ) prevented the insulin/IGF-I induced elevation of cMG1 mRNA, but not that induced by the other mitogens or the
stimulation of
mitogen activated protein kinase by
insulin
Lazar et al., J Biol Chem 1995
:
Insulin stimulates the activity of
mitogen activated protein kinase ( MAPK ) via its upstream activator, MAPK kinase ( MEK ), a dual specificity kinase that phosphorylates MAPK on threonine and tyrosine
Ohmichi et al., Endocrinology 1993
:
Despite the presence of functional receptors in these cells,
insulin does not
stimulate the activity of the
mitogen activated protein ( MAP ) kinase , despite a 5- to 8-fold activation observed with both NGF and EGF under the same conditions
Baron-Delage et al., Bull Cancer 1994
(Carcinoma...) :
As compared to vector control Caco-2 cells, both oncogene transfected cells exhibited : 1 ) a lost of response to
insulin 's stimulatory
effect on
mitogen activated protein ( MAP ) kinase activity and cell proliferation, both of which were constitutively increased ; 2 ) a decrease in insulin receptor (IR) affinity and insulin stimulated exogenous tyrosine kinase activity, which resulted, at least in part, from increased protein kinase C ( PKC ) activity ( 4 ), since both IR alterations were partially corrected by PKC down-regulation ; and 3 ) a decrease in both insulin receptor mRNA level and insulin receptor number, which was independent of PKC since it persisted after PKC down-regulation
Uehara et al., Biochem Biophys Res Commun 1995
:
Wortmannin inhibits
insulin induced Ras and
mitogen activated protein kinase activation related to adipocyte differentiation in 3T3-L1 fibroblasts ...
Insulin as a key factor in the process of the adipocyte differentiation
activated PI 3-kinase, Ras protein, and
mitogen activated protein kinase ( MAP kinase, ERK ) in 3T3-L1 fibroblasts
Chang et al., J Biol Chem 1995
:
We conclude that ( i ) transgenic expression of kinase-defective insulin receptors exerts dominant negative effects at the level of receptor auto-phosphorylation and kinase activation ; ( ii ) insulin receptor tyrosine kinase activity is required for in vivo
insulin stimulated IRS-1 phosphorylation, IRS-1 associated PI 3-kinase activation, phosphorylation of
mitogen activated protein kinase , and c-fos gene induction in skeletal muscle ; ( iii ) hybrid receptor formation is likely to contribute to the in vivo dominant negative effects of kinase-defective receptor expression
Sakaue et al., Mol Cell Biol 1995
:
In both CHO-IR and CHO-IR/SOS cells,
insulin rapidly
stimulated the formation of GTP bound Ras and the phosphorylation of
mitogen activated protein ( MAP ) kinase ; both these effects of insulin were markedly inhibited in CHO-IR/delta SOS cells
Yamamoto-Honda et al., J Biol Chem 1995
:
Although negative dominant Ras ( Ras17N ) induction in PC12 cells markedly decreased activities of
mitogen activated protein kinase ( MAP ) and pp90 S6 kinase in
response to
insulin or insulin-like growth factor I (IGF-I), activation of glycogen synthase by these agents was unaffected by negative dominant Ras induction
Ding et al., J Immunol 1993
:
c ) Macrophages from C3H/HeJ mice, which carry a defective Lps gene, fail to activate MAPK in response to taxol or LPS, although they activate
MAPK in
response to
insulin or IFN-gamma
Farese et al., Proc Natl Acad Sci U S A 1994
(Diabetes Mellitus, Type 2) :
In contrast to GPI dependent G3PAT activation, insulin stimulated hexose transport was intact in adipocytes and soleus and gastrocnemius muscles of the GK rat, as was
insulin induced activation of
mitogen activated protein kinase and protein kinase C
Baron-Delage et al., J Biol Chem 1994
(Colonic Neoplasms) :
As compared to vector control Caco-2 cells, both oncogene transfected cells exhibited : 1 ) a loss of response to
insulin 's stimulatory
effect on
mitogen activated protein ( MAP ) kinase activity and cell proliferation, both of which were constitutively increased ; 2 ) a decrease in insulin receptor (IR) affinity and insulin stimulated exogenous tyrosine kinase activity, which resulted from increased protein kinase C ( PKC ) activity ( Delage, S., Chastre, E., Empereur, S., Wicek, D., VeissiƩre, D., Capeau, J., Gespach, C., and Cherqui, G. ( 1993 ) Cancer Res. 53, 2762-2770 ), since IR alterations were corrected by PKC down-regulation ; and 3 ) a decrease in both IR mRNA level and IR number, which was independent of PKC since it persisted after PKC down-regulation
Milarski et al., J Biol Chem 1994
:
However, both the
insulin dependent tyrosine phosphorylation and activation of
mitogen activated protein kinase were markedly attenuated
Myers et al., Mol Cell Biol 1994
:
Coexpression of IRS-1 or IRS-1F-895 with the insulin receptor was required for insulin stimulated mitogenesis in 32-D cells, while expression of the insulin receptor alone was sufficient to mediate
insulin stimulated tyrosine phosphorylation of Shc and activation of p21ras and
mitogen activated protein ( MAP ) kinase
Yang et al., FEBS Lett 1993
:
Insulin and phorbol esters provoked 2- to 3-fold increases in MBPK/MAPK activity within 5-10 min. Whereas phorbol ester effects were blocked by protein kinase C ( PKC ) depletion or inhibition, insulin effects were fully intact, indicating that
insulin activates
MBPK/MAPK independently of PKC
Peraldi et al., Eur J Biochem 1993
:
Insulin and tumor promoting agent
regulate an inhibitor of the 44-kDa
mitogen activated protein kinase/extracellular signal regulated protein kinase 1 in fibroblasts
Knoepp et al., J Biol Chem 1996
:
Synergistic
effects of
insulin and phorbol ester on
mitogen activated protein kinase in Rat-1 HIR cells
Xu et al., Arch Biochem Biophys 1996
:
Lovastatin inhibits the
stimulation of
mitogen activated protein kinase by
insulin in HIRcB fibroblasts
Azpiazu et al., J Biol Chem 1996
:
Insulin increased the activities of
mitogen activated protein ( MAP ) kinase and the Mr = 90,000 isoform of ribosomal protein S6 kinase (Rsk) by approximately 1.5-2.0-fold
Yenush et al., Mol Cell Biol 1996
:
Insulin stimulated tyrosine autophosphorylation of the human insulin receptor and hDIR, and both receptors mediated tyrosine phosphorylation of Shc and
activated mitogen activated protein kinase
Todaka et al., Biochem J 1996
:
Overexpression of wild-type H-ras and the dominant negative mutant H-rass17N in our cell system respectively enhanced and blocked
insulin stimulated activation of
mitogen activated protein kinase , but did not affect insulin stimulated GLUT4 translocation
Ugi et al., J Biol Chem 1996
:
Consistently, the
insulin stimulated
mitogen activated protein kinase activities and DNA synthesis were also enhanced in wild-type cells, but impaired in mutant cells
Almind et al., J Clin Invest 1996
(Diabetes Mellitus, Type 2) :
There was also a tendency for a decrease in Grb2 binding to IRS-1 and
insulin stimulated
mitogen activated protein kinase activity, however, these were not statistically significant
DePaolo et al., Mol Cell Biol 1996
:
In contrast,
effects of
insulin on glycogen synthase and
mitogen activated protein kinase activity were inhibited only at higher concentrations of LY294002
Kole et al., J Biol Chem 1996
:
Furthermore, treatment of CHO/HIRc cells with this N-stearyl peptide led to a significant enhancement of the
insulin induced association of phosphatidylinositol (PI) 3-kinase activity with insulin receptor substrate 1 and the activation of
mitogen activated protein kinase
Begum et al., Eur J Biochem 1996
:
Prior treatment with TNF-alpha for 60 min blocked subsequent
insulin induced activation of
MAPK in a dose- and time dependent manner ... Metabolic labelling studies with inorganic [ 32P ] phosphate followed by immuno-precipitation of MAPK and its upstream activator, mitogen activated protein kinase kinase, indicated decreased phosphorylation of
MAPK and its kinase in
response to
insulin in cells exposed to TNF-alpha ... Low concentrations ( 2 nM ) of okadaic acid, a serine/threonine phosphatase inhibitor, prevented TNF-alpha induced inhibition of MAPK and restored
insulin 's
effect on
MAPK activity, while orthovanadate ( a tyrosine phosphatase inhibitor ), inhibitor 2 ( phosphatase-1 inhibitor ) and FK506 ( phosphatase-2B inhibitor ) were ineffective ... These results suggested an involvement of an okadaic-acid-sensitive serine/threonine phosphatase in TNF-alpha induced blockade of
insulin 's
effect on
MAPK and/or its kinase ... We conclude that TNF-alpha abrogates the
insulin effect on
MAPK activation by increasing dephosphorylation of MAPK kinase via an activated phosphatase
Porras et al., Int J Obes Relat Metab Disord 1996
:
Insulin treatment of untransfected 3T3 L1 cells also
induced quick activation of cytosolic 42 kDa mitogen activated protein kinase (
MAPK ) and a 90 kDa S6 kinase ( RSK ) ... Furthermore,
insulin induced
activation of
MAPK and RSK could be blocked by expression of a transfected, inducible dominant negative Ras mutant ( N17 )
Anderson et al., Kidney Int 1996
:
Ang II did not enhance insulin induced insulin receptor substrate-1 (IRS-1) phosporylation or phosphatidylinositol3 (PI-3) kinase activity, but did enhance
insulin induced
mitogen activated protein ( MAP ) kinase activity
Wattenberg et al., Biochem Biophys Res Commun 1996
:
In contrast to fumonisin B1,
insulin stimulates minimal activation of
MAPK in Swiss 3T3 cells
Kole et al., J Biol Chem 1996
:
Addition of stearyl peptide HC to CHO/HIRc cells resulted in a 2.4 +/- 0.3-fold increase in the amount of insulin stimulated phosphatidylinositol 3-kinase detected in anti-IRS-1 immunoprecipitates and a 2.1 +/- 0.6-fold increase in the levels of tyrosine phosphorylation of
mitogen activated protein kinase in
response to
insulin
Obata et al., Circ Res 1996
:
In our previous work, we showed that 10 nmol/L
insulin stimulated neither
mitogen activated protein kinase ( MAP kinase ) activity nor [ 3H ] thymidine incorporation but did stimulated S6 kinase through the specific insulin receptors in cultured rat vascular smooth muscle cells ( VSMCs )
Frevert et al., Mol Cell Biol 1997
:
Neither basal nor
insulin stimulated activity of glycogen synthase or
mitogen activated protein kinase was altered by iSH2-p110 coexpression
Shimizu et al., Endocrinology 1997
:
Furthermore, a selective inhibitor of phosphatidylinositol-3 kinase, wortmannin, did not inhibit the effect of NE, whereas
insulin induced activation of
MAPK was totally suppressed
Deuter-Reinhard et al., Mol Cell Biol 1997
:
Expression of SIP, but not catalytically inactive SIP, also blocked
insulin induced
mitogen activated protein kinase phosphorylation in oocytes
Suga et al., Diabetes 1997
:
Differential
activation of
mitogen activated protein kinase by
insulin and epidermal growth factor in 3T3-L1 adipocytes : a possible involvement of PI3-kinase in the activation of the MAP kinase by insulin
Kim et al., Biochem J 1997
:
Insulin regulation of mitogen activated protein kinase kinase ( MEK ),
mitogen activated protein kinase and casein kinase in the cell nucleus : a possible role in the regulation of gene expression
Bouaboula et al., J Biol Chem 1997
:
By contrast, SR 141716A had no effect on
MAPK activation by
insulin or IGF1 in CHO cells lacking CB1 receptors, ruling out the possibility of a direct interaction of SR 141716A with the Gi protein
Kusari et al., Mol Endocrinol 1997
:
Insulin induced
mitogen activated protein ( MAP) kinase phosphatase-1 (MKP-1 ) attenuates insulin stimulated MAP kinase activity : a mechanism for the feedback inhibition of insulin signaling
Sakaue et al., Mol Endocrinol 1997
:
Overexpression of a dominant negative RAS mutant in which Asp57 is replaced with tyrosine ( RAS57Y ) or of a dominant negative SOS mutant that lacks guanine nucleotide exchange activity ( delta SOS ) abolished the
insulin induced increase in
mitogen activated protein kinase activity, but had no effect on PI 3-kinase activity or glucose uptake
Burns et al., Biochem Biophys Res Commun 1997
:
Glucose stimulated
insulin secretion from rat islets of Langerhans is
independent of
mitogen activated protein kinase activation
Sharma et al., Mol Cell Biol 1997
:
Thus, interference with the IRS-1-IR interaction inhibits
insulin stimulated IRS-1 and Shc phosphorylation, PI 3-K enzymatic activity, p70s6k activation,
MAPK phosphorylation and cell cycle progression
Xi et al., FEBS Lett 1997
:
Insulin stimulated DNA synthesis,
MAP kinase ( MAPK ) activity and c-fos expression in vascular smooth muscle cells ( VSMCs ) was blocked by the MAPK inhibitor PD 98059
Begum et al., Metabolism 1998
(Diabetes Mellitus, Type 2...) :
The defects in PP-1/PP-2A activation/inactivation were accompanied by inhibition of
insulin 's
effect on
mitogen activated protein kinase ( MAPK ) activation
Farah et al., J Biol Chem 1998
:
Furthermore, microinjection of xROK-C mRNA inhibited
insulin induced
mitogen activated protein kinase activation with a concomitant inhibition of oocyte maturation
Takada et al., J Biol Chem 1998
:
The overexpression of wild-type SHPS-1, but not of a mutant SHPS-1 in which all four tyrosine residues in its cytoplasmic region were mutated to phenylalanine, markedly enhanced
insulin induced activation of
mitogen activated protein kinase in Chinese hamster ovary cells that overexpress the human insulin receptor
Hei et al., Mol Cell Biochem 1998
:
We observed a transient
activation of
MAPK and S6 kinases by
insulin in rat adipocytes, while both sodium selenate and vanadyl sulphate produced prolonged activation of the kinases ... Pretreatment of the cells with genistein did not affect the
activation of
MAPK by
insulin , but partially blocked the effects of sodium selenate and vanadyl sulphate
Guan et al., Am J Physiol 1998
:
Our data also indicate that both
insulin and IGF-I
enhance IL-1 beta induced p38 mitogen activated protein kinase (
MAPK ) phosphorylation and SAPK activation
Lin et al., Biochem Biophys Res Commun 1998
(Carcinoma, Hepatocellular...) :
Phosphatidylinositol 3-kinase is required for the regulation of hepatitis B surface antigen production and
mitogen activated protein kinase activation by
insulin but not by TPA
Porras et al., Mol Endocrinol 1998
:
Insulin-like growth factor I
(IGF-I)/insulin induced cytosolic p42/p44
mitogen activated protein kinase ( MAPK ) activation in a time dependent manner in fetal brown adipocytes, reaching a maximum at 5 min. Concurrently, nuclear p42/p44 MAPKs were also activated by IGF-I and insulin ... These results indicate that MEK mediates the
IGF-I/insulin induced p42/ p44
MAPK activation
Ulrich et al., Cell Biochem Funct 1998
:
Activation and glucagon
regulation of mitogen activated protein kinases (
MAPK ) by
insulin and epidermal growth factor in cultured rat and human hepatocytes ...
Insulin dependent activation of
MAPK was unequivocally observed only in human hepatocytes, though a slight activation was detected in rat ... Glucagon pretreatment blocked phosphorylation, gel mobility shift and kinase activity of
MAPK induced by
insulin but only partially blocked EGF induced MAPK activation in human hepatocytes
Sharma et al., J Biol Chem 1998
:
However,
insulin induced
mitogen activated protein kinase phosphorylation was inhibited
Agati et al., J Biol Chem 1998
:
Assessment of the
roles of
mitogen activated protein kinase , phosphatidylinositol 3-kinase, protein kinase B, and protein kinase C in
insulin inhibition of cAMP induced phosphoenolpyruvate carboxykinase gene transcription ... Thus,
insulin inhibition of PKA induced PEPCK expression does not
require MAPK activation but does require activation of PI3-kinase, although this signal is not transmitted through the PKB or PKC pathways
Summers et al., Mol Cell Biol 1998
:
C2-ceramide did not inhibit
insulin stimulated phosphorylation of
mitogen activated protein kinase or pp70 S6-kinase, and it actually stimulated phosphorylation of the latter in the absence of insulin
Begum et al., J Biol Chem 1998
:
Regulation of
mitogen activated protein kinase phosphatase-1 induction by
insulin in vascular smooth muscle cells
Navarro et al., Exp Cell Res 1998
:
Both phosphatidylinositol (PI) 3-kinase and mitogen activated protein kinase ( MAPK ) pathways are necessary for insulin/IGF-I full survival effect, since the use of specific inhibitors of PI 3-kinase activity ( wortmannin or LY294002, at the dose that inhibits PI 3-kinase activity induced by insulin ) or MAPK kinase activity inhibitor ( PD098059, at the dose that inhibits
insulin induced phosphorylation of
MAPK ) totally blocked the antiapoptotic effect induced by insulin/IGF-I, respectively
Myers et al., J Biol Chem 1998
:
Although several reports suggest a critical role for SHP-2 in
insulin stimulated
mitogen activated protein kinase activation and cell proliferation, IRS-1 ( FCT ) mediated these effects normally in 32D cells
Schechter et al., Brain Res 1998
:
Effects of brain endogenous
insulin on neurofilament and
MAPK in fetal rat neuron cell cultures
Yenush et al., Mol Cell Biol 1998
:
Insulin stimulated
mitogen activated protein kinase in 32D cells expressing insulin receptors ( 32DIR ) but failed to activate the phosphatidylinositol 3 (PI 3)-kinase cascade or to inhibit apoptosis