Gene interactions and pathways from curated databases and text-mining

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PIK3CA — PIK3R1

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Mainou et al., J Virol 2007 (Cell Transformation, Viral...) : CTAR1 is necessary for LMP1 mediated transformation as well as activation of PI3K signaling and induction of cell cycle markers associated with G ( 1 ) /S transition
Qiu et al., Reproduction 2004 (MAP Kinase Signaling System) : We showed for the first time that EGF activated both PI3K/Akt and MAPK/extracellular-signal regulated kinase ( ERK ) signalling in HTR8/SVneo, and increased both MMP-9 and TIMP-1 mRNAs and protein concentrations ... Our results suggest that the activation of both PI3K and MAPK pathways in extravillous trophoblasts is necessary for the up-regulation of MMP-9 and TIMP-1 expression by EGF ... Our results suggest that the activation of both PI3K and MAPK pathways in extravillous trophoblasts is necessary for the up-regulation of MMP-9 and TIMP-1 expression by EGF
Ihle et al., Mol Cancer Ther 2005 (Carcinoma, Non-Small-Cell Lung...) : Recent work shows that phosphatidylinositol-3-kinase (PI3-K) is coupled to the EGFR only in NSCLC cell lines expressing ErbB-3 and that EGFR inhibitors do not inhibit PI3-K signaling in these cells ... Recent work shows that phosphatidylinositol-3-kinase (PI3-K) is coupled to the EGFR only in NSCLC cell lines expressing ErbB-3 and that EGFR inhibitors do not inhibit PI3-K signaling in these cells
Tachado et al., PloS one 2008 : Furthermore, PI3K activation led to Akt stimulation, a serine/threonine kinase, which was also inhibited by wortmannin and LY294002
Nowak et al., eLife 2013 : We exploited mitotic recombination in developing Drosophila imaginal tissues to analyze the behavior of cells devoid of the tumor suppressor PTEN , a negative regulator of PI3K signaling, under varying nutritional conditions
Hanai et al., J Cell Biochem 2006 : Taken together, these results strongly suggest that PI3K/Akt negatively regulates the PDGF-BB stimulated IL-6 synthesis in osteoblasts
Finkielsztein et al., Biol Cell 2009 : Altering PI3K-Akt signalling in zebrafish embryos affects PTEN phosphorylation and gastrulation
Aquila et al., J Clin Endocrinol Metab 2005 : In uncapacitated sperm, both insulin and leptin increased PI3K activity, Akt S473, and glycogen synthase kinase-3 S9 phosphorylation ... In uncapacitated sperm, both insulin and leptin increased PI3K activity, Akt S473, and glycogen synthase kinase-3 S9 phosphorylation
Chalhoub et al., Annu Rev Pathol 2009 (Cell Transformation, Neoplastic...) : PTEN regulates PI3K signaling by dephosphorylating the lipid signaling intermediate PIP ( 3 ), but PTEN may have additional phosphatase independent activities, as well as other functions in the nucleus
Owada et al., PloS one 2013 : We have reported glucose deprivation rapidly induces AKT phosphorylation through PI3K activation
Zhang et al., Biochim Biophys Acta 2011 : Akt phosphorylation was inhibited by the PI3K inhibitor LY294002 ( 10µM ), farnyltranferase inhibitor FTI-276, or transfection with a dominant negative Akt
Chen et al., Sci China C Life Sci 2005 : Results show that both exogenous and endogenous EETs could remarkably enhance eNOS expression and its phosphorylation at Ser1179 and Thr497 residues ; PI3K inhibitor LY294002 could inhibit EETs induced increase in eNOS-Ser ( P ) 1179 but had no effect on the change of eNOS-Thr ( P ) 497, while Akt inhibitor could attenuate the increase in phosphor-eNOS at both residues ; both of the two inhibitors could block EETs enhanced eNOS expression
Kandasamy et al., Cancer Res 2002 (Carcinoma, Non-Small-Cell Lung...) : Furthermore, the loss of PTEN activity or overexpression of PI3-K dependent Akt/protein kinase B activity promotes the survival of NSCLC cells ... Furthermore, the loss of PTEN activity or overexpression of PI3-K dependent Akt/protein kinase B activity promotes the survival of NSCLC cells
Cao et al., Am J Physiol Cell Physiol 2006 : Thrombin also induced the expression of FGF-2 in a PI3K-Akt-Fra-1 dependent and mTOR independent manner, and neutralizing anti-FGF-2 antibodies inhibited thrombin stimulated VSMC DNA synthesis and motility
Gao et al., Am J Physiol Cell Physiol 2004 (Ovarian Neoplasms) : Expression of the cyclin kinase inhibitor p16(INK4a) was induced by the PI3K inhibitor, whereas steady-state levels of p21 ( CIP1/WAF1 ) were decreased in the same experiment ... Expression of the cyclin kinase inhibitor p16(INK4a) was induced by the PI3K inhibitor, whereas steady-state levels of p21 ( CIP1/WAF1 ) were decreased in the same experiment ... The inhibition of PI3K activity also inhibited the phosphorylation of AKT and p70S6K1 , but not extracellular regulated kinase 1/2 ... The inhibition of PI3K activity also inhibited the phosphorylation of AKT and p70S6K1, but not extracellular regulated kinase 1/2
Li et al., J Neuroimmunol 2011 (Chronic Disease...) : Furthermore, stimulation of TLR2 by PGN induces protection from stress induced reduction in the number of splenocytes through PI3K ... Collectively, our study thus demonstrates that stimulation of TLR2 mediated PI3K signaling attenuates splenocyte reduction induced by stress, and that ß-arrestin 2 modulates TLR2 mediated immune response following stress
Yoshii et al., Nat Neurosci 2007 : BDNF induces transport of PSD-95 to dendrites through PI3K-AKT signaling after NMDA receptor activation
Wu et al., J Neurochem 2004 : AMPA mediated neuroprotection is blocked by PP1 , an inhibitor of src family kinases, LY294002, a PI3-K inhibitor , or U0126, a MAPK kinase ( MEK ) inhibitor ... Thus, AMPA receptors protect neurons through a mechanism involving BDNF release, TrkB receptor activation, and a signaling pathway involving a PI3-K dependent activation of MAPK that increases BDNF expression
Tang et al., J Immunol 2007 : Leptin mediated IL-6 production was attenuated by OBRl receptor antisense oligonucleotide, PI3K inhibitor ( Ly294002 and wortmannin ), Akt inhibitor ( 1L-6-hydroxymethyl-chiro-inositol-2- ( ( R ) -2-O-methyl-3-O-octadecylcarbonate ) ), NF-kappaB inhibitor ( pyrrolidine dithiocarbamate ), IkappaB protease inhibitor ( L-1-tosylamido-2-phenylenylethyl chloromethyl ketone ), IkappaBalpha phosphorylation inhibitor ( Bay 117082 ), or NF-kappaB inhibitor peptide
Brady et al., Growth Horm IGF Res 2008 : IGF-I and -II caused significant increases in PI3-K , but not MAPK, activity ... IGF-I and -II caused significant increases in PI3-K , but not MAPK, activity
Baudhuin et al., Mol Pharmacol 2002 : LPA/S1P induced Akt activation may be involved in cell survival, because LPA and S1P treatment in HEY ovarian cancer cells results in a decrease in paclitaxel induced caspase-3 activity in a PI3-K/MEK/p38 dependent manner
Morton et al., Cell Metab 2005 (Body Weight) : These findings suggest that hypothalamic leptin signaling is an important determinant of glucose metabolism and that the underlying neuronal mechanism involves PI3K
Ding et al., Exp Cell Res 2008 : In addition, we have also analyzed the roles of PI3K and its downstream kinases Akt and atypical PKC in TRB3 expression
Berdichevsky et al., J Neurosci 2013 : Inhibition of PI3K and Akt prevented activation of mTOR , and was as effective as inhibition of mTOR in reducing ictal activity and cell death
Ke et al., J Hepatol 2012 (Disease Models, Animal...) : These findings underscore the role of HO-1 induced STAT3 in modulating PI3K/PTEN in liver IRI cascade
Welch et al., FEBS Lett 2003 (Second Messenger Systems) : Vice versa, Rac can also stimulate the activation of PI3K , although the mechanism for this is unclear
Song et al., Experimental biology and medicine (Maywood, N.J.) 2008 (Stomach Neoplasms) : Furthermore, PI3-K inhibitor LY294002 attenuated OPN mediated Akt activation
Villanueva et al., PloS one 2012 (Reperfusion Injury) : 8 ) Wortmannin, which inhibits ATM and PI3K , reduces hypoxia induced NFAT5 transcriptional activation in HEK293 cells
Nishimoto et al., J Neurosci Res 2008 (Brain Ischemia...) : These findings suggest that AMPA activates PI3K-Akt and subsequently inhibits GSK3beta and that inactivated GSK3beta attenuates glutamate induced caspase-3 cleavage and neurotoxicity
Bhattacharyya et al., Diabetologia 2012 (Glucose Intolerance...) : Carrageenan exposure completely inhibited insulin induced increases in phospho- ( Ser473 ) -Akt and PI3K activity in vivo in mouse liver and in human HepG2 cells
Komalavilas et al., J Appl Physiol 2001 : Phosphatidylinositol 3-kinase ( PI3-kinase ) activates protein kinase B ( also known as Akt ), which phosphorylates and activates a cyclic nucleotide phosphodiesterase 3B
Suzuki et al., J Biol Chem 2011 (Cell Transformation, Neoplastic...) : The levels of Cbp expression were inversely correlated with the activity of MEK and Akt, and Cbp down-regulation was suppressed by inhibiting MEK and PI3K
Yu et al., J Biol Chem 2002 : These experiments demonstrate that EGF and HGF mediated ERK activation result in divergent effects on Gab1/PI3K signaling ... These experiments demonstrate that EGF and HGF mediated ERK activation result in divergent effects on Gab1/PI3K signaling
Kanda et al., Biochem Biophys Res Commun 2003 : These results implicate CrkL in PI3K dependent activation of Rac by outside-in signaling from alpha4beta1 and suggest that adhesion through alpha4beta1 further activates cytokine receptor associated Jak2 to induce phosphorylation of these receptors
Tétreault et al., J Cell Physiol 2008 : Epidermal growth factor receptor dependent PI3K-activation promotes restitution of wounded human gastric epithelial monolayers ... In conclusion, the present results indicate that EGFR dependent PI3K activation promotes restitution of wounded human gastric epithelial monolayers
Chin et al., Am J Physiol Gastrointest Liver Physiol 2012 (Crohn Disease...) : Secretion was also inhibited by H-89 ( PKA inhibitor ) while Tph1 and VMAT1 transcription was regulated by PKA/MAPK and PI3K mediated signaling ... Secretion was also inhibited by H-89 ( PKA inhibitor ) while Tph1 and VMAT1 transcription was regulated by PKA/MAPK and PI3K mediated signaling
Merkenschlager et al., J Exp Med 2010 : These studies therefore explain the negative regulation of Foxp3 by PI3K signaling, and add Foxo proteins to the growing list of nuclear factors capable of modulating Foxp3 expression
Belo et al., Am J Physiol Gastrointest Liver Physiol 2011 (Colonic Neoplasms...) : Collectively, these findings indicate that ACh induced cell migration is mediated by MMP7 mediated release of HBEGF, an ERBB ligand that activates ERBB1 and downstream ERK and PI3K signaling
Fernandez-Gomez et al., Neurobiol Dis 2006 (Nerve Degeneration) : Moreover, phosphatidylinositol 3-kinase (PI3K) inhibitors attenuated pyruvate induced cytoprotection indicating that PI3K mediated Akt activation is necessary for pyruvate to induce cytoprotection
Zanou et al., J Biol Chem 2012 : Indeed, phosphorylation of both Akt and p70S6K proteins was decreased as well as the activation of PI3K , the main upstream regulator of the Akt
Carricaburu et al., Proc Natl Acad Sci U S A 2003 : PIP4K II beta expression did not impair insulin dependent association of PI3K with insulin receptor substrate 1 (IRS1) but abbreviated Akt activation, indicating that PIP4K II regulates PI-3,4,5-P3 degradation rather than synthesis ... PIP4K II beta expression did not impair insulin dependent association of PI3K with insulin receptor substrate 1 (IRS1) but abbreviated Akt activation , indicating that PIP4K II regulates PI-3,4,5-P3 degradation rather than synthesis ... PIP4K II beta expression did not impair insulin dependent association of PI3K with insulin receptor substrate 1 (IRS1) but abbreviated Akt activation, indicating that PIP4K II regulates PI-3,4,5-P3 degradation rather than synthesis
Galaria et al., J Surg Res 2005 : ATF mediated migration is PI3-K dependent and activates two separate pathways : ERK1/2 and akt ... ATF induces akt phosphorylation through a PI3K mediated but ras independent mechanism while both ras and PI3K are required for ERK1/2 activation ... ATF induces akt phosphorylation through a PI3K mediated but ras independent mechanism while both ras and PI3K are required for ERK1/2 activation
Demirjian et al., Biochim Biophys Acta 2006 (MAP Kinase Signaling System) : On the other hand, the PI3-K inhibitor, LY294002, and epidermal growth factor receptor (EGFR)-specific inhibitor, AG1478, did not suppress the release of TNF-alpha
MacKinnon et al., J Immunol 2008 : Galectin-3 binds to CD98, and exogenous galectin-3 or cross linking CD98 with the mAb 4F2 stimulates PI3K activation and alternative activation ... Galectin-3 binds to CD98, and exogenous galectin-3 or cross linking CD98 with the mAb 4F2 stimulates PI3K activation and alternative activation
Gayer et al., Am J Physiol Gastrointest Liver Physiol 2009 (Mechanotransduction, Cellular) : Blocking PI3K prevented strain stimulated ERK and p38 phosphorylation ... Blocking PI3K prevented strain stimulated ERK and p38 phosphorylation
Ghosh-Choudhury et al., J Biol Chem 2003 : Phosphatidylinositol 3-kinase regulates bone morphogenetic protein-2 (BMP-2) induced myocyte enhancer factor 2A-dependent transcription of BMP-2 gene in cardiomyocyte precursor cells ... Phosphatidylinositol 3-kinase regulates bone morphogenetic protein-2 (BMP-2) induced myocyte enhancer factor 2A-dependent transcription of BMP-2 gene in cardiomyocyte precursor cells
Murillo et al., Endocrinology 2001 (Disease Progression...) : At this stage, PI3K inhibition rapidly triggered accumulation of p27 ( kip1 ), cell cycle arrest, and cell death
Zeng et al., J Biol Chem 2002 : As expected, CDC42 and Rac1 activation mediated by EGLT can be completely inhibited by PI3K inhibitors, wortmannin and LY294002, and the p85 dominant negative mutant but not by either the phospholipase C inhibitor, or an intracellular Ca ( 2+ ) chilator BAPTA/AM ... As expected, CDC42 and Rac1 activation mediated by EGLT can be completely inhibited by PI3K inhibitors, wortmannin and LY294002, and the p85 dominant negative mutant but not by either the phospholipase C inhibitor, or an intracellular Ca ( 2+ ) chilator BAPTA/AM ... As expected, CDC42 and Rac1 activation mediated by EGLT can be completely inhibited by PI3K inhibitors, wortmannin and LY294002, and the p85 dominant negative mutant but not by either the phospholipase C inhibitor, or an intracellular Ca ( 2+ ) chilator BAPTA/AM
Wallin et al., Clin Cancer Res 2012 (Breast Neoplasms...) : GDC-0941 , a novel class I selective PI3K inhibitor , enhances the efficacy of docetaxel in human breast cancer models by increasing cell death in vitro and in vivo ... The current study was intended to determine whether GDC-0941 , an orally bioavailable class I selective PI3K inhibitor , enhances the antitumor activity of docetaxel in human breast cancer models in vitro and in vivo
Schmid et al., PloS one 2013 (Inflammation...) : We show here that PI3K? activates PLC? , leading to RasGrp/CalDAG-GEF-I & II mediated, Rap1a dependent activation of integrin a4ß1, extravasation of monocytes and granulocytes, and inflammation associated tumor progression ... Genetic depletion of PLC?, CalDAG-GEFI or II, Rap1a , or the Rap1 effector RIAM was sufficient to prevent integrin a4 activation by chemoattractants or activated PI3K? ( p110?CAAX ), while activated Rap ( RapV12 ) promoted constitutive integrin activation and cell adhesion that could only be blocked by inhibition of RIAM or integrin a4ß1 ... Genetic depletion of PLC?, CalDAG-GEFI or II, Rap1a, or the Rap1 effector RIAM was sufficient to prevent integrin a4 activation by chemoattractants or activated PI3K? ( p110?CAAX ), while activated Rap ( RapV12 ) promoted constitutive integrin activation and cell adhesion that could only be blocked by inhibition of RIAM or integrin a4ß1 ... Genetic depletion of PLC? , CalDAG-GEFI or II, Rap1a, or the Rap1 effector RIAM was sufficient to prevent integrin a4 activation by chemoattractants or activated PI3K? ( p110?CAAX ), while activated Rap ( RapV12 ) promoted constitutive integrin activation and cell adhesion that could only be blocked by inhibition of RIAM or integrin a4ß1 ... Genetic depletion of PLC?, CalDAG-GEFI or II, Rap1a, or the Rap1 effector RIAM was sufficient to prevent integrin a4 activation by chemoattractants or activated PI3K? ( p110?CAAX ), while activated Rap ( RapV12 ) promoted constitutive integrin activation and cell adhesion that could only be blocked by inhibition of RIAM or integrin a4ß1
Zhang et al., Experimental biology and medicine (Maywood, N.J.) 2010 : These findings demonstrate for the first time that PI3K/Akt dependent cyclin D1 activation plays an essential role in HDL induced EPC proliferation, migration and angiogenesis
Matheny et al., Biochem Biophys Res Commun 2009 : Our results collectively suggest that mTOR/p70S6K is activated in a PI3K/Akt dependent manner, but that in the absence of p110alpha or Akt, alternate pathway ( s ) may mediate activation of mTOR/p70S6K in C2C12 myoblasts ... Our results collectively suggest that mTOR/p70S6K is activated in a PI3K/Akt dependent manner, but that in the absence of p110alpha or Akt, alternate pathway ( s ) may mediate activation of mTOR/p70S6K in C2C12 myoblasts
Fong et al., J Cell Physiol 2008 (Chondrosarcoma) : Taken together, these results suggest that the BMP-2 acts through PI3K/Akt , which in turn activates IKKalpha/beta and NF-kappaB, resulting in the activations of beta1 integrin and contributing the migration of human chondrosarcoma cells
Facchini et al., FEBS Lett 2005 : In turn Src can activate PI3K and PKC-delta, and all these signaling proteins are required for ODC induction ... In turn Src can activate PI3K and PKC-delta, and all these signaling proteins are required for ODC induction
Berna et al., J Biol Chem 2010 : Akt activation by CCK and gastrin could be inhibited by the PI3K inhibitor wortmannin ... Akt activation by CCK and gastrin could be inhibited by the PI3K inhibitor wortmannin ... Akt activation by CCK and gastrin could be inhibited by the PI3K inhibitor wortmannin
Kurebayashi et al., Cell reports 2012 : Inhibition of PI3K-Akt-mTORC1-S6K1 axis impairs the downregulation of Gfi1 , a negative regulator of Th17 differentiation
Utomo et al., J Immunol 2006 : The FcgammaR mediated oxidative burst defect in Vav-deficient cells was linked to aberrant Rac activation as well as Rac- and actin-polymerization independent, but PI3K dependent , phosphorylation of the NADPH oxidase component p40(phox)
Tseng et al., J Cell Physiol 2010 : TNF-alpha increased the FAK, PI3K , and Akt phosphorylation
Zhang et al., Zhonghua Fu Chan Ke Za Zhi 2012 (Endometrial Neoplasms) : [ Effect of GPER on the activation of PI3K/Akt induced by 17ß-estradiol in endometrial carcinoma cells ]
Jackson et al., Virology 2010 : Surprisingly, given previous reports that NS1 activates PI3K to prevent apoptosis, the mutant viruses rUd-Y89F and rUd-P164/7A that fail to activate PI3K did not induce any more apoptosis than wild-type virus in MRC-5 and A549 cells, even though these cells are highly sensitive to inducers of apoptosis
Hatton et al., PloS one 2012 (Lymphoma, B-Cell) : Using small molecule inhibition and siRNA strategies in human B cell lines expressing a chimeric, signaling-inducible LMP1 protein, nerve growth factor receptor (NGFR)-LMP1, we show that NGFR-LMP1 utilizes Syk to activate PI3K/Akt signaling and induce IL-10 production ... Using small molecule inhibition and siRNA strategies in human B cell lines expressing a chimeric, signaling-inducible LMP1 protein, nerve growth factor receptor (NGFR)-LMP1, we show that NGFR-LMP1 utilizes Syk to activate PI3K/Akt signaling and induce IL-10 production ... Using small molecule inhibition and siRNA strategies in human B cell lines expressing a chimeric, signaling-inducible LMP1 protein, nerve growth factor receptor (NGFR)-LMP1, we show that NGFR-LMP1 utilizes Syk to activate PI3K/Akt signaling and induce IL-10 production ... Using small molecule inhibition and siRNA strategies in human B cell lines expressing a chimeric, signaling-inducible LMP1 protein, nerve growth factor receptor (NGFR)-LMP1, we show that NGFR-LMP1 utilizes Syk to activate PI3K/Akt signaling and induce IL-10 production ... Whereas Src kinases are often required for Syk activation, we show here that PI3K/Akt activation and autocrine IL-10 production by NGFR-LMP1 involves the Src family kinase Fyn ... Whereas Src kinases are often required for Syk activation, we show here that PI3K/Akt activation and autocrine IL-10 production by NGFR-LMP1 involves the Src family kinase Fyn ... Whereas Src kinases are often required for Syk activation, we show here that PI3K/Akt activation and autocrine IL-10 production by NGFR-LMP1 involves the Src family kinase Fyn
Maulik et al., J Cell Mol Med 2002 (Carcinoma, Small Cell...) : We show that association of c-Met with PI3K and GAB2 is diminished by inhibiting c-Met
Ueda et al., Blood 2002 (Calcium Signaling) : In Y719F, the binding of phosphatidylinositol 3'-kinase (PI3K) to KIT was lost and KIT mediated cell migration and Ca ( 2+ ) mobilization were suppressed by PI3K chemical inhibitors or dominant negative PI3K , suggesting the involvement of Y719 mediated PI3K pathway in cell migration ... In Y719F, the binding of phosphatidylinositol 3'-kinase (PI3K) to KIT was lost and KIT mediated cell migration and Ca ( 2+ ) mobilization were suppressed by PI3K chemical inhibitors or dominant negative PI3K , suggesting the involvement of Y719 mediated PI3K pathway in cell migration
Ramella et al., Regul Pept 2011 : Taken together, our data suggest a potential role of TPO as a physiological regulator of CF. By acting on specific receptors present on endothelial cells, TPO may induce PI3K/Akt dependent eNOS phosphorylation and NO release
Berna et al., Cell Signal 2009 (Pancreatic Neoplasms) : These effects are mediated by p85 phosphorylation and activation of PI3K
Janas et al., J Immunol 2011 : Mice with a mutant allele of p110? unable to bind active Ras revealed that CXCR4 mediated PI3K activation is Ras dependent
Bandara et al., PLoS Comput Biol 2009 : The experimental approach included the activation of endogenous phosphoinositide 3-kinase (PI3K) by chemically induced recruitment of a regulatory peptide, reversible inhibition of PI3K using a kinase inhibitor, and monitoring of the PI3K mediated production of PIP ( 3 ) lipids using the pleckstrin homology (PH) domain of Akt
Yu et al., Cell death & disease 2013 (Cell Transformation, Neoplastic...) : Our studies are the first to demonstrate that reduced CTGF as an unfavorable prognosis factor mediates the activation of miR-18b , an oncomir directly suppresses CTGF expression, by PI3K/AKT/C-Jun and C-Myc and promotes cell growth of NPC
Lai et al., International journal of biological sciences 2011 : Moreover, we demonstrated that IL-8 activated PI3K following the same kinetics observed from IL-8 induction of cytoskeletal rearrangement, suggesting the participation of PI3K in these processes
Jiang et al., Adv Cancer Res 2009 (Neoplasms...) : PI3K signaling regulates tumor growth and angiogenesis by activating AKT and other targets, and by inducing HIF-1 and VEGF expression ... PI3K signaling regulates tumor growth and angiogenesis by activating AKT and other targets, and by inducing HIF-1 and VEGF expression ... PI3K signaling regulates tumor growth and angiogenesis by activating AKT and other targets, and by inducing HIF-1 and VEGF expression
Thamilselvan et al., FASEB J 2007 (Colonic Neoplasms...) : PI3K inhibitor ( LY294002 ) prevented pressure stimulated Akt Ser473 and FAK Tyr397, but not FAK576 or Src416 phosphorylation ... PI3K inhibitor ( LY294002 ) prevented pressure stimulated Akt Ser473 and FAK Tyr397 , but not FAK576 or Src416 phosphorylation
Astoul et al., J Cell Biol 1999 : Moreover, PI3K signals are both necessary and sufficient for sustained activation of PKB in B lymphocytes
Bousquet et al., EMBO J 2006 (Neoplasms, Experimental) : Somatostatin , acting through sst2, inhibits PI3K activity by disrupting a pre existing complex comprising the sst2 receptor and the p85 PI3K regulatory subunit ... Mutating sst2-Y71 disabled sst2 to interact with p85 and somatostatin to inhibit PI3K , consequently abrogating sst2 's ability to suppress cell survival and tumor growth ... Mutating sst2-Y71 disabled sst2 to interact with p85 and somatostatin to inhibit PI3K , consequently abrogating sst2 's ability to suppress cell survival and tumor growth
Lau et al., Oncogene 2011 (Ovarian Neoplasms) : E-cadherin inhibits tumor cell growth by suppressing PI3K/Akt signaling via ß-catenin-Egr1 mediated PTEN expression ... Thus, the loss of E-cadherin itself may contribute to dysregulated PI3K/Akt signaling through its effects on PTEN, or it may exacerbate the frequent activation of PI3K/Akt signaling that occurs as a result of overexpression, mutation and/or amplification
Venkatesan et al., Cell Signal 2010 : Further, WISP1 stimulates PI3K-Akt dependent GSK3beta phosphorylation and beta-catenin nuclear translocation ... Further, WISP1 stimulates PI3K-Akt dependent GSK3beta phosphorylation and beta-catenin nuclear translocation
Ivins Zito et al., J Cell Physiol 2004 : Here, we show that overexpression of a catalytically inactive mutant of SHP-2 inhibited insulin-like growth factor-1 (IGF-1) dependent PI3K and Akt activation
Noh et al., Mol Med Report 2011 (Breast Neoplasms) : Inhibition of PI3K with an inhibitor, wortmannin, increased the level of PTEN protein in ERa positive MCF-7 cells, while levels in ERa negative MDA-MB 231 cells were not altered
Tanaka et al., J Biol Chem 2004 (MAP Kinase Signaling System...) : Here, we show that 1 ) nontoxic concentrations of TGF-beta1 activated Src kinase ; 2 ) TGF-beta1 rapidly phosphorylates ERK1/2 and Akt, but not p38 ; 3 ) pharmacological Src inhibitor PP2 or antisense ( AS ) c-Src oligodeoxynucleotide ( ODN ) treatment reduced TGF-beta1 induced phosphorylation of ERK1/2 and Akt by 85-90 % compared with controls ; 4 ) pharmacological inhibition of MAPK by PD98059 abrogated TGF-beta1 mediated Akt stimulation, whereas TGF-beta1 induced ERK1/2 stimulation was not inhibited by PI3K inhibitor LY294002 or AS-PI3K ODN transfection ; 5 ) up-regulation of uPA mRNA in response to TGF-beta1 was almost totally blocked by PP2 and PD98059 and partially ( approximately 55 % ) by LY294002 ; 6 ) TGF-beta1 induced uPA mRNA up-regulation was inhibited by treatment with AS ODNs to c-Src or PI3K by 90 or 60 %, respectively, compared with control ODN treatment ; and 7 ) blockade of the release of the transcription factor NF-kappaB by pyrrolidinedithiocarbamate reduced the TGF-beta1 induced activation of the uPA gene by approximately 65 %
Chaudhary et al., Toxicology 2004 : Pharmacological inhibition of phosphatidylinositol 3' kinase (PI3'K) by wortmannin and p38 mitogen activated protein kinase ( MAPK ) by SB203580 reveal that PI3'K mediates Be-specific p45 phosphorylation and IL6 release, whereas p38 MAPK regulates the release of IL6 and IL10 and the phosphorylation of p45 independent of metal-salt treatment ... Pharmacological inhibition of phosphatidylinositol 3' kinase (PI3'K) by wortmannin and p38 mitogen activated protein kinase ( MAPK ) by SB203580 reveal that PI3'K mediates Be-specific p45 phosphorylation and IL6 release, whereas p38 MAPK regulates the release of IL6 and IL10 and the phosphorylation of p45 independent of metal-salt treatment
Wang et al., J Biol Chem 2008 (Ovarian Neoplasms) : KLF8 promoter activity and mRNA levels are induced by expression of constitutively active ( CA ) phosphatidylinositol 3-kinase (PI3K) or CA-Akt but are repressed by dominant negative Akt or the PI3K inhibitor LY294002
Fresno Vara et al., Mol Biol Cell 2001 : In contrast, they inhibited the PRL dependent stimulation of the SFKs substrate Sam68, the phosphorylation of the tyrosine phosphatase Shp2, and the PI3K dependent Akt and p70S6k serine kinases ... In contrast, they inhibited the PRL dependent stimulation of the SFKs substrate Sam68, the phosphorylation of the tyrosine phosphatase Shp2, and the PI3K dependent Akt and p70S6k serine kinases
Engelman et al., J Clin Invest 2006 (Lung Neoplasms) : Moreover, continued activation of PI3K signaling by the PIK3CA oncogenic mutant, p110alpha E545K, was sufficient to abrogate gefitinib induced apoptosis
Jie et al., Mol Cell Biochem 2012 (Ion Channel Gating) : Taken together, these findings suggest that NRG-1 activates PI3K/Akt signaling and inhibits mPTP opening, and downstream apoptotic events in cardiac myocytes subjected to oxidative stress
Hagiwara et al., Nephrol Dial Transplant 2006 (Diabetes Mellitus, Type 2...) : EPA and specific inhibitors of ERK1/2, JNK and PI3K decreased levels of MCP-1 in MMCs. EPA suppressed phosphorylation of ERK1/2 and p38 in MMCs, and decreased p-ERK positive cells in glomeruli of KKAy/Ta mice ... EPA and specific inhibitors of ERK1/2, JNK and PI3K decreased levels of MCP-1 in MMCs. EPA suppressed phosphorylation of ERK1/2 and p38 in MMCs, and decreased p-ERK positive cells in glomeruli of KKAy/Ta mice
Kim et al., Biochem Biophys Res Commun 2010 : In addition, IBMX induced PPAR-gamma transcriptional activity was blocked by PI3K/Akt , PKA, or Rap1 inhibitors
Banerjee et al., Biochemistry 2008 (Breast Neoplasms) : Neutralization of VEGF action by its antibody or inhibition of VEGF induced PI3K/Akt kinase activation by wortmannin, a PI3K/Akt specific inhibitor, results in inhibition of VEGF induced hAOSMC migration
Liao et al., J Biol Chem 2011 (Breast Neoplasms) : PC4 functions are beyond TSA induced phosphatase release, PI3K mediated Sp1 phosphorylation, and HDAC1/2/mSin3A co-repressor release indicating its role as linker coactivator of Sp1 and the transcriptional machinery
Rotman et al., Reproduction 2010 : Previously, we reported that short-time activation of protein kinase A ( PRKA, PKA ) leads to PI3K activation and protein kinase C(alpha) ( PRKCA, PKC(alpha) ) inhibition ... Moreover, inhibition of PRKCA causes significant activation of PI3K
Abramova et al., Tsitologiia 2005 (Cell Transformation, Neoplastic) : Inhibition of PI3K leads to p27 ( Kip1 ) accumulation and cell cycle arrest, consequently
Li et al., Frontiers in bioscience : a journal and virtual library 2005 (Prostatic Neoplasms) : DIM also inhibited EGFR expression, PI3K kinase activity, and Akt activation, and abrogated the EGF induced activation of PI3K in prostate cancer cells
Min et al., J Immunol 2005 : It also led to the production of reactive oxygen species via PKC- and PI3K dependent activation of NADPH oxidase in the endothelial cells, and antioxidants suppressed the responses to TRANCE
Orita et al., Curr Eye Res 2013 : Conclusions : The poly ( I:C ) -induced secretion of IL-6, IP-10, and RANTES from human corneal fibroblasts is mediated by both NF-?B and PI3K signaling pathways, whereas that of IL-8 is mediated by the NF-?B pathway
Belaiba et al., Mol Biol Cell 2007 (Anoxia) : Together, these findings provide a novel mechanism in which hypoxia induces HIF-1alpha mRNA expression via the PI3K/AKT pathway and activation of NFkappaB ... Furthermore, inhibition of the phosphatidylinositol 3-kinase (PI3K)/AKT but not extracellular signal regulated kinase 1/2 pathway blocked the hypoxia dependent induction of HIF-1alpha mRNA and HIF-1alpha promoter activity, suggesting involvement of a PI3K/AKT regulated transcription factor
Fritz et al., J Immunol 2006 : Induction of VCAM-1 by OSM was diminished by pharmacological inhibitors of PI3K ( LY294002 ) but not inhibitors of ERK1/2 ( PD98059 ) or p38 MAPK ( SB203580 )
Tang et al., Biochem J 2003 (Astrocytoma...) : This response was blocked by rapamycin, but was not markedly affected by 100 nM wortmannin, implying separate roles for mTOR and PI3K in S6K1 activation
Pathak et al., J Immunol 2006 : TLR4/PI3K dependent ERK1/2 and p38 MAPK signaling converged upon activation of mitogen- and stress activated protein kinase 1 ( MSK1 ) ... TLR4/PI3K dependent ERK1/2 and p38 MAPK signaling converged upon activation of mitogen- and stress activated protein kinase 1 ( MSK1 )
Lin et al., J Biol Chem 2003 : Phosphatidylinositol 3-kinase , protein kinase C, and MEK1/2 kinase regulation of dopamine transporters (DAT) require N-terminal DAT phosphoacceptor sites
Brunt et al., Arterioscler Thromb Vasc Biol 2006 : Inhibition of PI3K-Akt reduced induction of HO-1 protein expression by H2O2 and blocked its anti-apoptotic effects
Wu et al., Particle and fibre toxicology 2012 (MAP Kinase Signaling System) : Pharmacological inhibition of ERK kinase and PI3K activity blocked DEP induced IL-8 and IL-1ß expression ... Pharmacological inhibition of ERK kinase and PI3K activity blocked DEP induced IL-8 and IL-1ß expression
Liu et al., Med Hypotheses 2013 (Prostatic Neoplasms) : More than 80 % of prostate tumors acquire mutation or deletion of tumor suppressor phosphatase and tensin homolog (PTEN) , a negative regulator of PI3K/Akt signaling
Conus et al., J Biol Chem 1998 : Furthermore, although PI3K in anti-phosphotyrosine immunoprecipitates is only very weakly activated by ionomycin, the calcium induced stimulation of p70 ( S6k ) is completely inhibited by the specific PI3K inhibitor wortmannin
Eulenfeld et al., J Cell Sci 2009 : We demonstrate that the presence of PI3K activity in the cell is not sufficient for binding Gab1 to the plasma membrane
Philpott et al., Proc Natl Acad Sci U S A 2004 : We propose that integrin mediated PI3K induction of NF-kappaB activates an autocrine TNF-alpha pathway required for DC maturation in response to Ad
Chi et al., Exp Cell Res 2010 (Lung Neoplasms) : Inhibitors of various MAPKs, PI-3K and PKC attenuated significantly IL-4 stimulated 15-PGDH activity indicating that MAPKs, PI-3K/Akt and PKC pathways participated in IL-4 induced up-regulation of 15-PGDH
Bhattacharyya et al., J Biol Chem 2011 (Cystic Fibrosis...) : However, overexpressing SHIP1 or inhibition of PI3K in CF cells suppressed IL-8 expression
Tu et al., Cancer Res 2000 (MAP Kinase Signaling System...) : IGF-I effectively activated PI 3-K in 8226 and OCI-My5 MM cells, but IL-6 was ineffective ... However, IL-6 successfully activated PI 3-K in AF-10 MM cells and IL-6 dependent MH.60 plasmacytoma/hybridoma cells ... IGF-I also successfully activated PI 3-K in four of four MM patient specimens, and IL-6 activated PI 3-K in three of four specimens ... IGF-I also successfully activated PI 3-K in four of four MM patient specimens, and IL-6 activated PI 3-K in three of four specimens
Shortt et al., Blood 2013 (Lymphoma, B-Cell) : Moreover, apoptosis was initiated at drug concentrations insufficient to antagonize PI3K/mTORC2 regulated AKT phosphorylation
Kang et al., Stem Cells Dev 2005 : bFGF did not induce activation of PI3K or PKC, but induced activation of ERK ( extracellular signal regulated kinase )
Otero et al., J Biol Chem 2001 (Lymphoma, B-Cell) : A prominent feature of CD19 signaling is the binding and activation of phosphoinositide 3-kinase ( P13K ), which accounts for the majority of PI3K activity induced by BCR ligation
Aikin et al., Endocrinology 2004 : In addition, IGF-I suppressed cytokine mediated JNK activation in a PI3K dependent manner ... In addition, IGF-I suppressed cytokine mediated JNK activation in a PI3K dependent manner
Margolis et al., J Physiol 2011 : Using a variety of blockers we determined that the augmentation is probably due to insertion of GABA ( A ) Rs into the synapse by a mechanism that is G-protein independent and mediated by activation of Akt via PI3K
Kelley et al., J Biol Chem 1999 : In normal human monocytes, M-CSF increased the levels of tyrosine phosphorylated proteins and induced Akt activation in a PI3K dependent manner ... In normal human monocytes, M-CSF increased the levels of tyrosine phosphorylated proteins and induced Akt activation in a PI3K dependent manner
Wang et al., American journal of physiology. Renal physiology 2001 : In A6 kidney cells, mineralocorticoid induction of the phosphorylated form of SGK preceded the increase in Na+ transport, and specific inhibition of PI3K inhibited both phosphorylation of SGK and mineralocorticoid induced Na+ transport
Ma et al., Biochem Pharmacol 2004 : Furthermore, DEP promoted phosphorylation of Akt , a substrate of phosphatidylinositol 3-kinase (PI3K), on Ser-473 and Thr-308 in a PI3K dependent manner, and enhanced phosphorylation of down-stream p70/p85 S6 kinases ( p70/p85S6K ) as well as glycogen synthase kinase-3beta ( GSK-3beta ) ... Furthermore, DEP promoted phosphorylation of Akt, a substrate of phosphatidylinositol 3-kinase (PI3K), on Ser-473 and Thr-308 in a PI3K dependent manner, and enhanced phosphorylation of down-stream p70/p85 S6 kinases ( p70/p85S6K ) as well as glycogen synthase kinase-3beta ( GSK-3beta )
Yalcin et al., Oncogene 2010 (Neoplasms, Experimental) : Selective inhibition of choline kinase simultaneously attenuates MAPK and PI3K/AKT signaling
Acconcia et al., IUBMB Life 2003 : At the present meagre information is available for the role played by the rapid hormone action mechanism ( s ) ( i.e., activation of ERK , PI(3)K , PKC-alpha ) in modulating E2-induced gene promoter activity
Dai et al., Folia Biol (Praha) 2010 : Importantly, PI3K/Akt enhanced GRP78 accumulation through increasing its stability following endoplasmic reticulum stress
Monfar et al., Mol Cell Biol 1995 : We demonstrate that these kinases lie on the same signalling pathway and that PI3K mediates the activation of pp70 by the cytokine interleukin-2 (IL-2) ... Like rapamycin, PKA appears to act downstream of cPKC mediated pp70S6k activation, and like wortmannin, PKA antagonizes IL-2 dependent activation of PI3K
Pham et al., Circ Res 2000 : One mechanism involves PI3K/PKB dependent phosphorylation of 4E-BP1 , which dissociates from eIF4E, allowing initiation of translation from the 7-methylGTP cap of mRNAs ... Insulin or H ( 2 ) O ( 2 ) increased the phosphorylation ( activation ) of PKB through PI3K , but, whereas insulin induced a sustained response, the response to H ( 2 ) O ( 2 ) was transient
Venieratos et al., Cell Signal 2010 : Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K mediated Akt phosphorylation suppressed by high glucose
Yeh et al., Biochem Pharmacol 2008 (Chondrosarcoma) : Taken together, these results suggest that the TGF-beta1 acts through PI3K/Akt , which in turn activates IKKalpha/beta and NF-kappaB, resulting in the activations of alphavbeta3 integrins and contributing the migration of chondrosarcoma cells
Zhao et al., Am J Pathol 2010 (Brain Neoplasms...) : An EGF receptor (EGFR) kinase inhibitor, a RasN17 dominant negative construct, MEK and PI3K inhibitors significantly blocked EGF/EGFR stimulated MMP-9 , cell invasion, and colony formation in U1242 MG cells, suggesting that MMP-9 is involved in EGFR/Ras/MEK and PI3K/AKT signaling pathway mediated cell invasion and anchorage independent growth in U1242 MG cells
Dufour et al., Bone 2008 : Biochemical and molecular analyses revealed that the attenuated PI3K signaling induced by FGFR2 activation is due to increased Cbl-PI3K molecular interaction mediated by the Cbl Y731 residue, which results in increased PI3K ubiquitination and proteasome degradation ... Biochemical and molecular analyses revealed that the attenuated PI3K signaling induced by FGFR2 activation is due to increased Cbl-PI3K molecular interaction mediated by the Cbl Y731 residue, which results in increased PI3K ubiquitination and proteasome degradation
Eda et al., Cell Biol Int 2011 (Arthritis, Rheumatoid) : The aim of this study is to investigate whether PI3K ( phosphatidylinositol-3-kinase ) is involved in IL-1ß ( interleukin-1ß ) -induced IL-6 production in A549 ( human lung adenocarcinoma epithelial cell ) and human RASF ( rheumatoid arthritis synovial fibroblast ) ... PI3K inhibitor, LY294002 significantly reduced IL-1ß induced IL-6 production in A549 cells but not in RASF, indicating that IL-1ß induced IL-6 production was partially mediated by PI3Kin A549 cells but not in RASF ... These results indicate that IL-1ß induced IL-6 production in A549 cells is mediated by both PI3K and IRAK4 and suggest that involvement of PI3K in the IL-1 induced IL-6 production is cell type specific
Mhashilkar et al., Mol Ther 2003 (Breast Neoplasms...) : Expression of the PI3K pathway members ( p85 PI3K , FAK, ILK-1, Akt, and PLC-gamma ) was downregulated and expression of the PI3K antagonist PTEN was increased
Varma et al., Am J Physiol Heart Circ Physiol 2005 (Hyperglycemia) : We conclude that d-glucose regulates Akt signaling through threonine phosphorylation of Akt and that hyperglycemia impaired PI3k-Akt signaling may promote EC proliferative dysfunction in diabetes
Rodríguez-Pérez et al., Int J Biochem Cell Biol 2009 : Inhibitors of PI3K and PKC essentially blocked insulin-, IGF-I- and EGF induced desensitizations
Jossin et al., Mol Cell Biol 2007 : We show that the mTor ( mammalian target of rapamycin ) -S6K1 ( S6 kinase 1 ) pathway is activated by Reelin and that this depends on Dab1 ( Disabled-1 ) phosphorylation and activation of PI3K and Akt ( protein kinase B )
Perron et al., Neural development 2011 : BMP6 , which evokes neural induction but does not have orienting activity, activates Smad signaling but does not stimulate PI3K
Zhang et al., Front Biosci (Elite Ed) 2011 : Taken together, the present study demonstrates that T3 potentiates the cell survival action of FSH through Src- and PI3K mediated Xiap up-regulation and decreased Fas and FasL expression ... Taken together, the present study demonstrates that T3 potentiates the cell survival action of FSH through Src- and PI3K mediated Xiap up-regulation and decreased Fas and FasL expression ... Taken together, the present study demonstrates that T3 potentiates the cell survival action of FSH through Src- and PI3K mediated Xiap up-regulation and decreased Fas and FasL expression
Ruggiero et al., BMC molecular biology 2007 : Our study identified a set of transcripts that are targets of KSRP and whose expression is increased by PI3K-AKT activation
Tu et al., J Biol Chem 2008 : Inhibition of CaR expression blocked the Ca ( 2+ ) ( o ) -induced tyrosine phosphorylation of beta-, gamma-, and p120-catenin, PI3K, and the tyrosine kinase Fyn and the association of Fyn with E-cadherin and PI3K
Hamburger et al., Anticancer Res 1997 : The purpose of these experiments was to determine the effects of heregulin beta 1 (HRG) on growth and PI3K activation of a nonneoplastic human mammary epithelial cell line, 184B5 ... Similarly, HRG at all concentrations stimulated the association of PI3K with erbB3 ... We conclude that both the growth inhibitory and stimulatory effects of HRG in nonneoplastic human mammary epithelial cells are mediated by activation of PI3K
Tung et al., Cell Signal 2007 : In this study, we identified a requirement for PDGFR, PI3-K/Akt , p38 MAPK, JNK, and NF-kappaB in the regulation of VCAM-1 expression by rat vascular smooth muscle cells ( VSMCs ) in response to viral infection
Chapuis et al., Haematologica 2010 (Blast Crisis...) : Autocrine IGF-1/IGF-1R signaling is responsible for constitutive PI3K/Akt activation in acute myeloid leukemia : therapeutic value of neutralizing anti-IGF-1R antibody ... As we previously reported IGF-1 autocriny in acute myeloid leukemia cells, we investigated whether IGF-1 signaling was involved in the constitutive activation of PI3K
Chuang et al., Endocrinology 2007 (Diabetes Mellitus, Experimental...) : MAPK/ERK inhibitor PD98059 inhibited the PI3K activity, Akt phosphorylation, and lipid accumulation triggered by HG ... MAPK/ERK inhibitor PD98059 inhibited the PI3K activity, Akt phosphorylation, and lipid accumulation triggered by HG ... PI3K inhibitor LY294002 did not affect the HG-increased ERK1/2 phosphorylation during adipogenesis
Chacko et al., J Biol Chem 1996 : activation of platelets with anti-Fc gamma RIIA antibodies resulted in the noncovalent association of PI 3-K with Fc gamma RIIA as well as an increase in Fc gamma RIIA associated PI 3-K activity
Sizemore et al., Mol Cell Biol 1999 : In contrast, PI3K inhibitors block the IL-1 stimulated phosphorylation of NF-kappaB itself, especially the p65/RelA subunit
Alonzi et al., Mol Cell Neurosci 2001 : To assess the relative importance of these pathways in promoting the survival of cytokine dependent neurons, we conditionally inactivated STAT3 in mice and inhibited MEK, PI3K , and Akt in cultured neurons using pharmacological reagents and by expressing specific inhibitory proteins
Heijink et al., Eur J Immunol 2003 : Together, our results indicate that increased IL-2 dependent PI3-K signaling leads to impaired negative feedback control of the production of Th2-type cytokine IL-5 by the cAMP dependent pathway ... Together, our results indicate that increased IL-2 dependent PI3-K signaling leads to impaired negative feedback control of the production of Th2-type cytokine IL-5 by the cAMP dependent pathway
Capodici et al., J Immunol 1998 : Aggregation of human neutrophils is associated with activation of the mitogen activated protein kinases Erk1 and -2, and Erk is activated in response to PI 3-K in some cell types
Baki et al., J Neurosci 2008 (Alzheimer Disease...) : Expression of exogenous WT PS1 or constitutively active Akt in PS1-/- neurons stimulates PI3K signaling and suppresses both caspase-3 activity and dendrite retraction ... Expression of exogenous WT PS1 or constitutively active Akt in PS1-/- neurons stimulates PI3K signaling and suppresses both caspase-3 activity and dendrite retraction ... Familial Alzheimer disease ( FAD ) mutations suppress the ability of PS1 to promote PI3K/AKT signaling, prevent phosphorylation/inactivation of GSK-3 and promote activation of caspase-3
Sherry et al., J Immunol 2007 (Diabetes Mellitus, Type 2) : LPS dependent stimulation of PI3K activity, ERK1/2 activation, and p38 kinase activity was greater in PerMphi from db/db mice as compared with db/+ mice
Suh et al., J Cell Physiol 2009 : In addition, IL-6 increased EGFR/src/FAK, PI3K/Akt phosphorylation and 2-DG uptake as well as GLUT-2 protein expression, which were blocked by AG 1478 ( EGF receptor inhibitor ), PP2 ( src family of tyrosine kinase inhibitor ), PI3K-specific siRNA, and a Akt inhibitor
Dilly et al., Invest Ophthalmol Vis Sci 2008 (Disease Models, Animal) : Light stress induced the activation of PI3K through activation and binding of IGF-1R , which leads to activation of the Akt survival pathway in photoreceptors
Tenbaum et al., Nat Med 2012 (Colonic Neoplasms...) : Simultaneous hyperactivation of the Wnt-ß-catenin pathway and inhibition of PI3K-AKT signaling promote nuclear accumulation of ß-catenin and FOXO3a , respectively, promoting cell scattering and metastasis by regulating a defined set of target genes ... In the presence of high nuclear ß-catenin content, activation of FOXO3a by PI3K or AKT inhibitors makes it behave as a metastasis inductor rather than a proapoptotic tumor suppressor
Tuo et al., Am J Physiol Cell Physiol 2009 : Therefore, the possible involvement of PI3K in the regulation of cAMP- and cGMP induced duodenal epithelial CFTR activation was investigated in the present study ... Forskolin and 8-Br-cGMP not only increased the activity of PI3K but also induced the phosphorylation of Akt , a signaling molecule downstream of PI3K, which were again inhibited by wortmannin and LY294002
Jiang et al., Mol Cell Biol 2004 (Cell Transformation, Neoplastic...) : Ectopic expression of RhoB, but not the close relative RhoA , inhibits Ras, PI3K , and Akt induction of transformation, migration, and invasion and induces apoptosis and anoikis ... Ectopic expression of RhoB , but not the close relative RhoA, inhibits Ras, PI3K , and Akt induction of transformation, migration, and invasion and induces apoptosis and anoikis
Gauthier et al., Am J Physiol Cell Physiol 2001 (MAP Kinase Signaling System) : To investigate whether human intestinal epithelial cell survival involves distinct control mechanisms depending on the state of differentiation, we analyzed the in vitro effects of insulin , pharmacological inhibitors of Fak, MEK/Erk, and PI3-K/Akt , and integrin ( beta1, beta4 ) -blocking antibodies on the survival of the well established human Caco-2 enterocyte-like and HIEC-6 cryptlike cell models ... Herein, we report that 1 ) the enterocytic differentiation process results in the establishment of distinct profiles of Bcl-2 homolog expression levels, as well as p125(Fak), p42 ( Erk-2 ), and p57 ( Akt ) activated levels ; 2 ) the inhibition of Fak, of the MEK/Erk pathway, or of PI3-K, have distinct impacts on enterocytic cell survival in undifferentiated ( subconfluent Caco-2, confluent HIEC-6 ) and differentiated ( 30 days postconfluent Caco-2 ) cells ; 3 ) exposure to insulin and the inhibition of Fak, MEK, and PI3-K resulted in differentiation state-distinct modulations in the expression of each Bcl-2 homolog analyzed ; and 4 ) Fak, beta1 and beta4 integrins, as well as the MEK/Erk and PI3-K/Akt pathways, are distinctively involved in cell survival depending on the state of cell differentiation ... Herein, we report that 1 ) the enterocytic differentiation process results in the establishment of distinct profiles of Bcl-2 homolog expression levels, as well as p125(Fak), p42 ( Erk-2 ), and p57 ( Akt ) activated levels ; 2 ) the inhibition of Fak, of the MEK/Erk pathway, or of PI3-K, have distinct impacts on enterocytic cell survival in undifferentiated ( subconfluent Caco-2, confluent HIEC-6 ) and differentiated ( 30 days postconfluent Caco-2 ) cells ; 3 ) exposure to insulin and the inhibition of Fak, MEK, and PI3-K resulted in differentiation state-distinct modulations in the expression of each Bcl-2 homolog analyzed ; and 4 ) Fak, beta1 and beta4 integrins, as well as the MEK/Erk and PI3-K/Akt pathways, are distinctively involved in cell survival depending on the state of cell differentiation
Shair et al., Cancer Res 2008 : However, constitutive activation of Akt alone did not alter cell growth, suggesting that both PI3K/Akt and NF-kappaB activation are required by LMP1
Kong et al., Eur J Pharmacol 2013 (Diabetes Mellitus) : Additionally, we found that FGF21 induced PKC?/? phosphorylation in a PI3K dependent manner ; and that a non-isoform selective PKC inhibitor blocked FGF21 inhibition of glucose production, while an inhibitor of classical and novel PKC isoforms had no effect on FGF21 inhibitory activity
Lipson et al., J Pharmacol Exp Ther 1998 : A synthetic EGF receptor kinase inhibitor showed selective inhibitor properties when tested for EGF induced receptor autophosphorylation, MAPK activation, PI3K activation, entry into S phase and cyclin E-associated kinase activity
Slomiany et al., IUBMB Life 2005 : The impedance by leptin of the LPS inhibitory effect on mucin synthesis was blocked by wortmannin, an inhibitor of PI3K , as well as by ERK inhibitor, PD98059
Schmidt et al., BMC biology 2004 (MAP Kinase Signaling System) : Surprisingly, three structurally different PI3K inhibitors block Ras, MEK and Erk activation in PEPs by Epo
Gentle et al., J Immunol 2012 : Modulation required de novo protein synthesis, and PI3K, JNK, and ERK activity were necessary for enhanced IL-2 expression, whereas modulation of IL-10 required only PI3K activity ... Modulation required de novo protein synthesis, and PI3K , JNK, and ERK activity were necessary for enhanced IL-2 expression, whereas modulation of IL-10 required only PI3K activity
Zhang et al., Wei Sheng Yan Jiu 2008 : [ Involvement of EGF receptor and PI3K in ICAM-1 expression in human airway epithelial cells exposed to zinc sulfate ] ... The expression of ICAM-1 mRNA and protein and activation of epithelial growth factor receptor (EGFR) and PI3K was determined using PCR and immunoblotting
O'Farrell et al., PLoS Biol 2013 : Both Stit and insulin receptor (InR) signaling activate PI3K-I and drive cellular proliferation and tissue growth
García et al., Mol Endocrinol 2002 : PI3K is involved in the IGF-I inhibition of TSH induced sodium/iodide symporter gene expression ... PI3K is involved in the IGF-I inhibition of TSH induced sodium/iodide symporter gene expression ... Interestingly, inhibition of PI3K blocked IGF-I repression of TSHinduced NIS mRNA and protein levels
Méndez-Samperio et al., Cell Immunol 2007 (Tuberculosis) : Importantly, the PI3K activation was dependent on SPK
Lu et al., J Immunol 1998 : Phosphatidylinositol 3-kinase is required for CD28 but not CD3 regulation of the TEC family tyrosine kinase EMT/ITK/TSK : functional and physical interaction of EMT with phosphatidylinositol 3-kinase ... CD28 induced association of EMT with PI3K also requires functional expression of LCK ... CD28 induced association of EMT with PI3K also requires functional expression of LCK
Lavery et al., J Neurosci 2007 : Our results raise the possibility that neurofibroma formation in individuals with neurofibromatosis might result in part from a Ras-PI3K-Akt dependent inhibition of FOXO within Schwann cells
Wadhone et al., J Immunol 2009 (Leishmaniasis, Visceral) : Miltefosine induced protein kinase C-dependent and PI3K dependent p38MAP kinase phosphorylation and anti-leishmanial function
Friedrichsen et al., J Endocrinol 2006 : We conclude that incretin induced beta-cell replication is dependent on cAMP/PKA, p42 MAPK and PI3K activities, which may involve transcriptional induction of cyclin D1
Reistad et al., Toxicological sciences : an official journal of the Society of Toxicology 2005 : We suggest that tyrosine kinase mediated activation of PI3K could result in enhanced activation of calcium dependent PKC by enhanced PLC activity, followed by intracellular calcium release leading to ROS formation in neutrophil granulocytes
Wang et al., J Surg Res 2009 (Breast Neoplasms) : Moreover, a combination of TZDs and a specific Akt inhibitor may serve as a new approach to target Wnt/beta-catenin directly and via PI3K/Akt action on glycogen synthase-3beta
Bénéteau et al., Mol Cancer Res 2008 : Herein, we show that inhibition of the PI3K signal by edelfosine triggers a Fas mediated apoptotic signal independently of the Fas/FasL interaction
Maldonado et al., Biochem Biophys Res Commun 2005 (Calcium Signaling) : IGF-1 induced CREB phosphorylation was mediated by MEK1/ERK, PI3-K , p38-MAPK, as well as Ca ( 2+ ) /calmodulin kinase and calcineurin
Yang et al., J Biol Chem 2005 : In contrast, PI3K does not mediate IFNalpha/beta induced p100 processing, although PI3K is involved in the pathway resulting in IkappaB alpha degradation ... In contrast, PI3K does not mediate IFNalpha/beta induced p100 processing, although PI3K is involved in the pathway resulting in IkappaB alpha degradation ... Thus, IFN induces NF-kappaB activation to mediate IFN dependent cell survival signals through a `` canonical '' pathway of IkappaB alpha proteolysis mediated by PI3K/Akt and a `` noncanonical '' pathway of p100 processing mediated by NIK/TRAF
Cao et al., PLoS Biol 2013 (Angelman Syndrome) : In Ube3A deficient mice, the BDNF induced recruitment of PSD-95, as well as PLC? and Grb2 associated binder 1 (Gab1) with TrkB receptors was attenuated, resulting in reduced activation of PLC?-a-calcium/calmodulin dependent protein kinase II ( CaMKII ) and PI3K-Akt , but leaving the extracellular signal regulated kinase ( Erk ) pathway intact ... In Ube3A deficient mice, the BDNF induced recruitment of PSD-95, as well as PLC? and Grb2 associated binder 1 (Gab1) with TrkB receptors was attenuated, resulting in reduced activation of PLC?-a-calcium/calmodulin dependent protein kinase II ( CaMKII ) and PI3K-Akt , but leaving the extracellular signal regulated kinase ( Erk ) pathway intact
Li et al., Cell Death Differ 2007 (Neuroblastoma) : Previously, we found BDNF activation of TrkB through PI3K/Akt protects NB from etoposide/cisplatin induced cell death ... Previously, we found BDNF activation of TrkB through PI3K/Akt protects NB from etoposide/cisplatin induced cell death
Saegusa et al., Mol Immunol 2007 : The enhancement effect of PI3K inhibitors on IL-12 p70 production almost completely disappeared by the treatment with anti-IL-10 mAb ... The enhancement effect of PI3K inhibitors on IL-12 p70 production almost completely disappeared by the treatment with anti-IL-10 mAb ... PI3K inhibitors suppressed the activation of extracellular regulated kinase (ERK) , a member of the mitogen activated protein kinases, by CpG-ODN ... A specific ERK inhibitor, U0126, as well as PI3K inhibitors, differentially regulated IL-10 and IL-12 p70 productions ... A specific ERK inhibitor, U0126, as well as PI3K inhibitors, differentially regulated IL-10 and IL-12 p70 productions ... These results suggest that PI3K positively and negatively regulates the production of CpG-ODN induced IL-10 and IL-12 p70 , respectively, and negatively regulates IL-12 p70 production in macrophages through ERK mediated IL-10 induction ... These results suggest that PI3K positively and negatively regulates the production of CpG-ODN induced IL-10 and IL-12 p70, respectively, and negatively regulates IL-12 p70 production in macrophages through ERK mediated IL-10 induction ... These results suggest that PI3K positively and negatively regulates the production of CpG-ODN induced IL-10 and IL-12 p70, respectively, and negatively regulates IL-12 p70 production in macrophages through ERK mediated IL-10 induction
Chaudhary et al., J Cell Biochem 2001 : Wortmannin ( 500 nM ), a specific inhibitor of phosphatidylinositol 3-kinase ( PI 3-K), inhibited the activation of ERK1 and ERK2 by PDGF-BB but not by FGF-2 suggesting that PI 3-K mediated the activation of ERK MAPK pathway by PDGF-BB but not by FGF-2 ... Wortmannin ( 500 nM ), a specific inhibitor of phosphatidylinositol 3-kinase ( PI 3-K), inhibited the activation of ERK1 and ERK2 by PDGF-BB but not by FGF-2 suggesting that PI 3-K mediated the activation of ERK MAPK pathway by PDGF-BB but not by FGF-2 ... Taken together, our data for the first time revealed that the activation of ERK1/2 by PDGF-BB is mediated by PI 3-K , and secondly, Akt is activated by PDGF-BB and EGF but not by FGF-2 in human and mouse osteoblastic cells ... Taken together, our data for the first time revealed that the activation of ERK1/2 by PDGF-BB is mediated by PI 3-K , and secondly, Akt is activated by PDGF-BB and EGF but not by FGF-2 in human and mouse osteoblastic cells
Alladina et al., J Vasc Res 2005 : Inhibition of PI3K reduces p-Akt , with concurrent reductions in c-FLIP ( S ) and Bcl-2, and so renders endothelium sensitive to TRAIL induced apoptosis through the extrinsic and intrinsic pathways
Kawanabe et al., Mol Pharmacol 2003 : These results indicate that 1 ) Ca ( 2+ ) influx through NSCC-1, NSCC-2, and SOCC plays essential roles in ET-1 induced PYK2 phosphorylation, 2 ) NSCC-2 and SOCC are stimulated by ET-1 via a PI3K dependent cascade, whereas NSCC-1 is stimulated via a PI3K independent cascade, and 3 ) PI3K is involved in the PYK2 phosphorylation that depends on Ca ( 2+ ) influx through SOCC and NSCC-2
Zhou et al., Arterioscler Thromb Vasc Biol 2003 : LY294002, a PI3K inhibitor, and N-acetylcysteine, a scavenger of reactive oxygen species, inhibited the stretch activation of Akt
Coso et al., PloS one 2012 : Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS, PLC?1, and Erk1/2 ... Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS, PLC?1, and Erk1/2 ... Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS , PLC?1, and Erk1/2 ... Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS, PLC?1 , and Erk1/2 ... Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS, PLC?1, and Erk1/2 ... Furthermore, activation of PI3K/Akt by VEGF-C/VEGFR-3 resulted in phosphorylation of P70S6K, eNOS, PLC?1, and Erk1/2
Altiok et al., J Biol Chem 1999 (Breast Neoplasms) : In the absence of heregulin, the ectopic expression of the constitutively active p110 subunit of PI3K was sufficient to induce BRCA1 phosphorylation
Elsegood et al., Arterioscler Thromb Vasc Biol 2009 : MEK1/2 and PI3K activities were also required for oxLDL induced Bcl-2 and Bcl-x ( L ) mRNA upregulation ... MEK1/2 and PI3K activities were also required for oxLDL induced Bcl-2 and Bcl-x ( L ) mRNA upregulation
Bhowmick et al., J Virol 2013 (Rotavirus Infections) : This function of NSP1 was independent of its interferon or phosphatidylinositol 3-kinase (PI3K)/AKT modulation activity since p53 degradation was observed in Vero cells as well as in the presence of PI3K inhibitor
Wong et al., Biotechnol Bioeng 2006 : The phosphoinositide 3-kinase (PI3K) inhibitor, wortmannin, totally blocked the effect of both zinc and insulin on Akt activation, indicating the involvement of PI3K in the activation of Akt by zinc, rather than zinc acting on Akt directly
Xia et al., Journal of thrombosis and haemostasis : JTH 2010 : Subsequently, PI3K overexpression activated Rac1 and increased ROS generation ... Protein kinase Czeta mediates PI3K regulated NADPH oxidase activation by promoting cellular p47phox translocation
Chung et al., J Endocrinol 2013 (MAP Kinase Signaling System) : Our data also suggest that PI3K/Akt mediated inactivation of GSK-3ß and activation of mTOR/p70 ( S6K ) contribute to the proliferative effect of ghrelin ... Our data also suggest that PI3K/Akt mediated inactivation of GSK-3ß and activation of mTOR/p70 ( S6K ) contribute to the proliferative effect of ghrelin
John et al., Dev Biol 2008 : Oocyte-specific ablation of Pten resulted in PI3K induced Akt activation, Foxo3 hyperphosphorylation, and Foxo3 nuclear export, thereby triggering primordial follicle activation, defining the steps by which the PI3K pathway and Foxo3 control this process ... Oocyte-specific ablation of Pten resulted in PI3K induced Akt activation , Foxo3 hyperphosphorylation, and Foxo3 nuclear export, thereby triggering primordial follicle activation, defining the steps by which the PI3K pathway and Foxo3 control this process
Jang et al., Cell Signal 2005 : Interestingly, there was PI3K dependent activation of AKT , p70S6K, JNKs, and NF-kappaB in response to catalase ... Interestingly, there was PI3K dependent activation of AKT, p70S6K , JNKs, and NF-kappaB in response to catalase ... Interestingly, there was PI3K dependent activation of AKT, p70S6K, JNKs, and NF-kappaB in response to catalase
Ramos-Nino et al., Molecular cancer 2007 (Mesothelioma) : Cell lines with PI3K dependent Fra-1 expression were SV40 positive and expressed the lowest basal Fra-1 levels
Fang et al., Nat Immunol 2001 : Proteolysis independent regulation of PI3K by Cbl-b mediated ubiquitination in T cells
Yu et al., PloS one 2012 (Disease Models, Animal...) : Pre-treatment with PI3K inhibitor wortmannin or LY294002 prevented activation of spinal AKT induced by ephrinB1-Fc. Inhibition of spinal PI3K signaling dose-dependently prevented and reversed pain behaviors and spinal c-Fos protein expression induced by intrathecal injection of ephrinB1-Fc. Inhibition of EphBs receptors by intrathecal injection of EphB1-Fc reduced formalin induced inflammation and chronic constrictive injury induced neuropathic pain behaviors accompanied by decreased expression of spinal PI3K, p-AKT and c-Fos protein ... Pre-treatment with PI3K inhibitor wortmannin or LY294002 prevented activation of spinal AKT induced by ephrinB1-Fc. Inhibition of spinal PI3K signaling dose-dependently prevented and reversed pain behaviors and spinal c-Fos protein expression induced by intrathecal injection of ephrinB1-Fc. Inhibition of EphBs receptors by intrathecal injection of EphB1-Fc reduced formalin induced inflammation and chronic constrictive injury induced neuropathic pain behaviors accompanied by decreased expression of spinal PI3K, p-AKT and c-Fos protein
Berger et al., Biochem Biophys Res Commun 1994 : Phosphatidylinositol 3-kinase ( PI 3-kinase ) is acutely stimulated by insulin but its role in regulating glucose metabolism is still not fully understood
Chen et al., EMBO J 2011 (Prostatic Neoplasms) : Importantly, we find that the molecular mechanism underlying MED1 mediated chromatin looping involves PI3K/AKT phosphorylated MED1 mediated recruitment of FoxA1, RNA polymerase II and TATA binding protein and their subsequent interactions at the UBE2C locus
Suuronen et al., Neurochem Int 2006 (Inflammation) : Our studies on signaling pathways revealed that PI3K inhibitors LY294002 and Wortmannin blocked the TSA induced pro-inflammatory response but surprisingly did not affect the okadaic acid induced response
Ito et al., Anim Sci J 2010 : In this study the effect of a PI3K inhibitor, LY294002, on the MAPK and p34 ( cdc2 ) kinase activities of matured porcine oocytes was examined
Koh et al., Brain Res Mol Brain Res 2005 : To evaluate the protective effects of low concentration of DADS on oxidative stress injured nPC12 cells, the viability of the cells ( pretreated with DADS for 2 h vs. not pretreated ) was evaluated 24 h after exposure to 100 microM H2O2 for 30 min. Compared to the cells treated with 100 microM H2O2 only, pretreatment of the cells with 20 microM DADS before exposure to 100 microM H2O2 increased the viability and induced activation of PI3K and Akt, inactivation of GSK-3, and inhibition of cytochrome c release, caspase-3 activation , and PARP cleavage ... These results indicate that low concentration of DADS has neuroprotective effects by activating PI3K/Akt and by inhibiting GSK-3 activation, cytochrome c release, caspase-3 activation, and PARP cleavage, whereas high concentration is rather cytotoxic
Alvin et al., Med Sci Monit 2011 (Cardiomegaly) : However in the absence of angiotensin converting enzyme inhibition, ANG II increased both IK and IK1 in a PI3-K dependent manner in hypertrophied cardiomyocytes ... Thus, captopril treatment reveals a negative effect of ANG II on IK and IK1, which is PI3-K independent, whereas in the absence of angiotensin converting enzyme inhibition IK and IK1 regulation is dependent upon PI3-K
Lin et al., Mol Pharmacol 2003 : PTX blocks Akt but not phosphatidylinositol 3-kinase (PI3K) activation in response to PDGF and abrogates cyclin D1 induction by PI3K , suggesting an effect of PTX on Akt itself
Lucas et al., Int J Oncol 2002 (Cell Transformation, Neoplastic) : Modulation of phospholipase D by Ras proteins mediated by its effectors Ral-GDS, PI3K and Raf-1
Xu et al., Mol Med Report 2013 : However, LY294002, a PI3K inhibitor, attenuated the anti-apoptotic effect of salidroside and blocked the increase of Akt and mTOR ; however, did not affect the antioxidative effect of salidroside ... However, LY294002, a PI3K inhibitor, attenuated the anti-apoptotic effect of salidroside and blocked the increase of Akt and mTOR ; however, did not affect the antioxidative effect of salidroside
Liu et al., PloS one 2013 (Pleural Effusion...) : The induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt and p38 signaling pathway
Yoshimi et al., Blood 2011 (Leukemia) : Evi1 represses PTEN expression and activates PI3K/AKT/mTOR via interactions with polycomb proteins
Kirk-Ballard et al., PloS one 2013 (Diabetes Mellitus, Experimental...) : The effect of PMI5011 is mediated by PI3K/Akt signaling and correlates with decreased expression of Atrogin-1 and MuRF-1
Li et al., Am J Transplant 2012 (Arteriosclerosis) : Conditioned media from Ltv-p53 transferred SMCs activated PI3K/Akt/mTOR and MAPK/Erk signaling in a SDF-1a dependent manner and thereby promoted mesenchymal stem cell (MSC) migration and proliferation
Jin et al., J Biol Chem 2008 (Cell Transformation, Neoplastic) : Here we define some aspects of the molecular mechanisms regulating TrkC dependent Ras-Erk1/2 and PI3K/Akt activation ... Suppression of c-Src by RNA interference in highly metastatic 4T1 mammary cancer cells, which express endogenous TrkC, resulted in markedly decreased expression of cyclin D1 and suppression of activation of Ras-Erk1/2 and PI3K-Akt
Quon et al., Biochem Biophys Res Commun 1996 : In adipose cells, stimulation of endogenous PDGF receptors (PDGF-R) results in increased PI3K activity without causing recruitment of GLUT4 ... Our data suggest that overexpression of PDGF-R mediates positive effects on GLUT4 translocation by a wortmannin sensitive pathway not dependent on direct interaction of the PDGF-R with PI3K
Mavila et al., PloS one 2012 (Liver Neoplasms) : FGF Receptor ( R ) activation resulted in the downstream activation of MAPK, PI3K-AKT , and ß-catenin pathways, as well as cellular proliferation
Park et al., J Biol Chem 2007 : Specifically, NGF enhanced TrkA phosphorylation, PI3K activity, and Akt phosphorylation
Tang et al., J Biol Chem 2000 : Activation of PI3K leads to enhanced activity of PKB and increased levels of the anti-apoptotic protein Bcl-X ( L )
Komori , Clinical calcium 2006 : Phosphoinositide 3-kinase (PI3K)-Akt signaling enhances DNA binding of Runx2 and Runx2 dependent transcription, and Runx2 upregulates PI3K subunits ( p85 and p110 beta ) and Akt ... Phosphoinositide 3-kinase (PI3K)-Akt signaling enhances DNA binding of Runx2 and Runx2 dependent transcription, and Runx2 upregulates PI3K subunits ( p85 and p110 beta ) and Akt ... Phosphoinositide 3-kinase (PI3K)-Akt signaling enhances DNA binding of Runx2 and Runx2 dependent transcription, and Runx2 upregulates PI3K subunits ( p85 and p110 beta ) and Akt
Skorski et al., Blood 1995 (Blast Crisis...) : Phosphatidylinositol-3 kinase activity is regulated by BCR/ABL and is required for the growth of Philadelphia chromosome positive cells ... Phosphatidylinositol-3 kinase activity is regulated by BCR/ABL and is required for the growth of Philadelphia chromosome positive cells
Soda et al., Cell Signal 2008 : Previously we and others have shown that TPO activates PI3K and Akt and that this pathway is important for megakaryocyte growth
Bechard et al., Mol Cell Biol 2012 : These findings provide new links between PI3K/Akt signaling and regulation of Gsk3ß activity by Frat , an oncogene previously shown to cooperate with Myc in tumorigenesis
Maeda et al., Biochem Biophys Res Commun 2004 : Expression of Gab1 PI3K-m in SK-N-MC human primitive neuroectodermal tumor cells expressing wild-type RET markedly impaired Akt phosphorylation, Rac1 activation, and lamellipodia formation that were induced by GDNF whereas expression of Gab1 SHP2-m partially impaired Erk activation ... Expression of Gab1 PI3K-m in SK-N-MC human primitive neuroectodermal tumor cells expressing wild-type RET markedly impaired Akt phosphorylation, Rac1 activation, and lamellipodia formation that were induced by GDNF whereas expression of Gab1 SHP2-m partially impaired Erk activation
Chae et al., J Neurophysiol 2005 : NRG1- and TGF(beta)1 evoked stimulation of K ( Ca ) is blocked by inhibitors of PI3K by overexpression of a dominant negative form of Akt, by overexpression of CTMP, an endogenous negative regulator of Akt, and by application of the Akt inhibitor 1L-6-hydroxymethyl-chiro-inositol 2- ( R ) -2-O-methyl-3-O-octadecylcarbonate ( HIMO )
Jang et al., Biochem Biophys Res Commun 2004 : Catalase also induced activation of NF-kappaB, PI3K , ERKs, p38s, or JNKs. Catalase induced COX-2 expression was abrogated by treatment of MG-132 ( a NF-kappaB inhibitor ) or LY294002 ( a PI3K inhibitor ), but not by treatment of PD98059 ( an ERK inhibitor ), SB203580 ( a p38 inhibitor ), or SP600125 ( a JNK inhibitor ) ... Moreover, inhibition of PI3K by LY294002 caused partial decrease of catalase induced COX-2 transcription and steady-state COX-2 transcript levels, but not COX-2 mRNA stability
Cao et al., Cell Signal 2008 : Inhibition of EGFR also suppresses UV-induced activation of PI3K/AKT/mTOR/S6K and NF-kappaB signal transduction pathways
Wheeler et al., Cancer Biol Ther 2009 (Carcinoma, Non-Small-Cell Lung...) : We now present data that Src family kinases (SFKs) are highly activated in cetuximab-resistant cells and enhance EGFR activation of HER3 and PI(3)K/Akt
Gallacher et al., J Physiol 2009 (Influenza, Human) : This response to virus infection was only partially dependent upon NS1 mediated activation of PI3K
Lasunskaia et al., J Leukoc Biol 2006 (Tuberculosis) : Mycobacteria directly induce cytoskeletal rearrangements for macrophage spreading and polarization through TLR2 dependent PI3K signaling ... The obtained data demonstrate that the direct effect of mycobacteria on macrophage shape might be mediated through TLR2 dependent PI3K activation
Sumitani et al., Endocrinology 2002 : PI3K activity, but not mTOR, may regulate transcriptional activity of myogenin
Sun et al., Proc Natl Acad Sci U S A 2008 : Consistent with a TSC-Rheb-PLD signaling cascade, AMPK and PI3K , both established regulators of TSC2 , appear to lie upstream of PLD as revealed by the effects of pharmacological inhibitors, and serum activation of PLD is also dependent on amino acid sufficiency
Shin et al., J Immunol 2009 : Consistent with this, B. burgdorferi activation of MyD88 or TLR3/TRIF signaling resulted in increased activity of PI3K ... Consistent with this, B. burgdorferi activation of MyD88 or TLR3/TRIF signaling resulted in increased activity of PI3K
Haimsohn et al., Endocrinology 2002 (Breast Neoplasms) : 4 ) The insulin receptor substrate-1/2 associated PI3K , Shc proteins, and the kinases Akt and Erk1/2, downstream mediators of the antiapoptotic signaling by IGF-IR, were activated to a higher extent and for a longer time period by ATA , compared with IGF-I
Zhang et al., J Cell Mol Med 2011 (Endotoxemia...) : To determine whether PI3K regulates Rac1 activation, cardiomyocytes were treated with LY294002, a PI3K selective inhibitor ... We conclude that PI3K mediated Rac1 activation is required for induction of TNF-a expression in cardiomyocytes and cardiac dysfunction during endotoxemia ... We conclude that PI3K mediated Rac1 activation is required for induction of TNF-a expression in cardiomyocytes and cardiac dysfunction during endotoxemia
Feng et al., J Cell Biochem 2011 : PI3K and ERK/Nrf2 pathways are involved in oleanolic acid induced heme oxygenase-1 expression in rat vascular smooth muscle cells
Hu et al., Diabetes 2007 (Diabetes Mellitus, Experimental) : In cultured C2C12 myotubes, acute suppression of PI3K activity led to decreased PTEN expression, while palmitic acid increased PTEN in myotubes in a p38 dependent fashion
Boo et al., Am J Physiol Heart Circ Physiol 2002 : These results suggest that shear stress stimulates eNOS by two different mechanisms : 1 ) PKA- and PI3K dependent and 2 ) PKA dependent but PI3K independent pathways
Corey et al., J Neurochem 2010 : Phosphoinositide 3-kinase (PI3K) activity has been implicated physiologically in olfactory signal transduction, suggesting a potential role for a G protein coupled receptor activated class I PI3K
Jana et al., J Immunol 2007 : Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha
Malyala et al., J Comp Neurol 2008 : We found that the PI3K inhibitors wortmannin and LY294002 significantly reduced the estrogen mediated GABA(B) receptor desensitization in POMC arcuate neurons, suggesting that PI3K signaling is a critical downstream mediator of the estrogen mediated rapid effects
Blank et al., Exp Cell Res 2013 : In conclusion, we herein demonstrated that STAT1 serine phosphorylation is mediated by the sequential activation of PI3K , PKCd and p38 MAPK
Ma et al., Mol Endocrinol 2007 : BRCA1 underexpression also enhanced estrogen-inducible ER-alpha activity in a PI3K/Akt dependent manner
Akl et al., Planta Med 2012 (Breast Neoplasms...) : Western blot studies revealed that combined low-dose treatment of ?-tocotrienol and sesamin caused a marked reduction in EGF induced ErbB3 and ErbB4 receptors phosphorylation ( activation ) and a relatively large decrease in intracellular levels of total and/or phosphorylated c-Raf, MEK1/2, ERK1/2, PI3K , PDK1, Akt, p-NF?B, Jak1, Jak2, and Stat1, as compared to cells treated with only one compound or in the vehicle treated control group
Birkenkamp et al., Exp Hematol 2000 (Acute Disease...) : An inhibitor of PI3-K differentially affects proliferation and IL-6 protein secretion in normal and leukemic myeloid cells depending on the stage of differentiation
Solinas et al., FEBS Lett 2004 : Our results suggest that the direct effect of leptin in stimulating thermogenesis in skeletal muscle is mediated by substrate cycling between de novo lipogenesis and lipid oxidation, and that this cycle requires both PI3K and AMPK signaling ... Our results suggest that the direct effect of leptin in stimulating thermogenesis in skeletal muscle is mediated by substrate cycling between de novo lipogenesis and lipid oxidation, and that this cycle requires both PI3K and AMPK signaling
Lim et al., J Neurosci Res 2008 : Thus, MAPK/ERK dependent p35 up-regulation and MAPK/ERK dependent and PI3K/Akt dependent Bcl2 up-regulation contribute to BDNF stimulated neural differentiation and to the survival of differentiated cells
Wheeler et al., Mol Cell Biol 2001 : Interaction with Grb2 dramatically increased the catalytic activity of this PI3K
Chen et al., Nephron. Experimental nephrology 2009 : ALD inhibited the activity of PI3-K/Akt and increased the activation of p38MAPK ... ALD induces apoptosis in rat podocytes through inhibition of PI3-K/Akt and stimulation of p38 MAPK signaling pathways
Jang et al., Br J Pharmacol 2013 (Wounds, Penetrating) : Both an AMPK inhibitor ( compound C ) anda HO-1 activity inhibitor ( SnPPIX ) but not inhibitors of MAPKs, PI3K and PKC reduced the production of VEGF by CKD712
Tal-Or et al., J Cell Biochem 2006 : The PI3K inhibitor LY294002 and the MEK inhibitor PD98059 caused a partial inhibition of the Ras induced ErbB4 receptor phosphorylation
Tang et al., Int J Biochem Cell Biol 2012 : Therefore, we conclude that PI3K activated by Epac leads to the activation of PKB/CREB signaling and the up-regulation of PPAR? expression, which in turn activate the transcription of adipogenic genes ; whereas osteogenesis is driven by Rho/FAK/MEK/ERK/Runx2 signaling, which can be inhibited by Epac via PI3K ... Therefore, we conclude that PI3K activated by Epac leads to the activation of PKB/CREB signaling and the up-regulation of PPAR? expression, which in turn activate the transcription of adipogenic genes ; whereas osteogenesis is driven by Rho/FAK/MEK/ERK/Runx2 signaling, which can be inhibited by Epac via PI3K
Etgen et al., Endocrinology 2003 : These data suggest that activation of both PI3K and MAPK by E ( 2 ) and IGF-I mediates hormonal facilitation of lordosis behavior
Pan et al., Mol Nutr Food Res 2008 : We found that 6-shogaol also inhibited LPS induced activation of PI3K/Akt and extracellular signal regulated kinase 1/2, but not p38 mitogen activated protein kinase ( MAPK )
Xiang et al., Cell Biochem Funct 2011 : The inhibitory effect of ghrelin on caspase-3 activity was attenuated by inhibitors of ERK1/2 ( PD98059 ), PI3K/Akt ( LY294002 ) and growth hormone secretagogue receptor ( GHSR ) -1a ( D-Lys ( 3 ) -growth hormone releasing peptide-6 )
Lee et al., Int Immunopharmacol 2008 : Interestingly, emodin induced the activation of phosphatidylinositol 3-kinase (PI3K), Akt and mitogen activated protein ( MAP ) kinases, but those inductions by emodin were completely inhibited by the PI3K inhibitor, LY294002, suggesting that the up-regulation of BMP-2 by emodin could be mediated through the activation of both Akt and MAP kinases by activating PI3K ... Interestingly, emodin induced the activation of phosphatidylinositol 3-kinase (PI3K), Akt and mitogen activated protein ( MAP ) kinases, but those inductions by emodin were completely inhibited by the PI3K inhibitor, LY294002, suggesting that the up-regulation of BMP-2 by emodin could be mediated through the activation of both Akt and MAP kinases by activating PI3K ... Interestingly, emodin induced the activation of phosphatidylinositol 3-kinase (PI3K), Akt and mitogen activated protein ( MAP ) kinases , but those inductions by emodin were completely inhibited by the PI3K inhibitor, LY294002, suggesting that the up-regulation of BMP-2 by emodin could be mediated through the activation of both Akt and MAP kinases by activating PI3K ... Interestingly, emodin induced the activation of phosphatidylinositol 3-kinase (PI3K) , Akt and mitogen activated protein ( MAP ) kinases, but those inductions by emodin were completely inhibited by the PI3K inhibitor, LY294002, suggesting that the up-regulation of BMP-2 by emodin could be mediated through the activation of both Akt and MAP kinases by activating PI3K
Georgopoulos et al., PloS one 2010 : Functional inactivation of E-cadherin interferes with the capacity of NHU cells to form stable calcium mediated contacts, attenuates E-cadherin mediated PI3-K/AKT induction and enhances NHU cell proliferation by allowing de-repression of the EGFR/ERK pathway and constitutive activation of ß-catenin-TCF signalling
Kim et al., Mediators Inflamm 2010 : Activation of CD147 with cyclophilin a induces the expression of IFITM1 through ERK and PI3K in THP-1 cells ... Activation of CD147 with cyclophilin a induces the expression of IFITM1 through ERK and PI3K in THP-1 cells ... CD147 induced expression of IFITM1 was blocked by inhibitors of ERK, PI3K , or NF-?B, but not by inhibitors of p38, JNK, or PKC
Takasawa et al., FEBS Lett 2006 : The Reg/ATF-2 induced cyclin D1 promoter activation was attenuated by PI(3)K inhibitors such as LY294002 and wortmannin
Unni et al., Breast Cancer Res 2005 (MAP Kinase Signaling System...) : PI3-K activity was inhibited by MSC followed by dephosphorylation of Akt ... MSC inhibits cell growth by inhibiting the activity of PI3-K and its downstream effector molecules in mouse mammary tumor cells in vitro
Halvorsen et al., Brain Res 2002 (MAP Kinase Signaling System...) : Survival promoting MEK-ERK and PI3K pathways contribute to viability during MPP ( + ) exposure, both are activated by NGF , and loss of PI3K mediated signaling and NF-kappa B-mediated transcription may commit cells irreversibly to apoptosis in this model
Bracke et al., J Leukoc Biol 2000 (Asthma...) : Moreover, inhibition of PI3K in these cells blocked the background and the TNF-alpha induced IgA binding completely
Zhang et al., J Biol Chem 2006 : In this study we investigated a potential link between the PI3K-Akt pathway and the cigarette smoke ( CS ) -stimulated epidermal growth factor receptor mediated FRA-1 induction in non-oncogenic HBE cells ... In this study we investigated a potential link between the PI3K-Akt pathway and the cigarette smoke ( CS ) -stimulated epidermal growth factor receptor mediated FRA-1 induction in non-oncogenic HBE cells ... We found that the PI3K through p21 activated kinase 1 regulates FRA-1 proto-oncogene induction by CS and the subsequent activation of the Elk1 and cAMP-response element binding protein transcription factors that are bound to the promoter in HBE cells ... We found that the PI3K through p21 activated kinase 1 regulates FRA-1 proto-oncogene induction by CS and the subsequent activation of the Elk1 and cAMP-response element binding protein transcription factors that are bound to the promoter in HBE cells
Rahmani et al., Cancer Res 2013 (Leukemia, Myeloid) : PI3K/mTOR inhibitors markedly downregulated Mcl-1 but increased Bim binding to Bcl-2/Bcl-xL ; the latter effect was abrogated by ABT-737 ... PI3K/mTOR inhibitors markedly downregulated Mcl-1 but increased Bim binding to Bcl-2/Bcl-xL ; the latter effect was abrogated by ABT-737
Schneider et al., PloS one 2008 : CTLA-4 ligation induced PI 3K activation as evidenced by the phosphorylation of PKB/AKT that in turn inactivated GSK-3 ... CTLA-4 induced PI 3K and AKT activation also led to phosphorylation of the pro-apoptotic factor BAD as well as the up-regulation of BcL-XL
Chamaon et al., Biochem Biophys Res Commun 2005 (Astrocytoma) : This suggest, that PI3K and MAPK signalling is involved in PTTG regulation not only in malignant astrocytomas but also in non-tumorous cells
Guo et al., J Biol Chem 2011 (Breast Neoplasms...) : IKBKE activation of Akt was not affected by inhibition of PI3K , knockdown of PDK1 or mTORC2 complex ... IKBKE activation of Akt was not affected by inhibition of PI3K , knockdown of PDK1 or mTORC2 complex
Takayama et al., Int Immunol 2013 (MAP Kinase Signaling System) : We also found that the extracellular signal regulated kinase ( ERK ) signaling pathway was activated in a PI3K dependent manner upon FceRI stimulation and that simultaneous inhibition of Akt and ERK resulted in nearly complete blockade of FceRI induced degranulation ... We also found that the extracellular signal regulated kinase ( ERK ) signaling pathway was activated in a PI3K dependent manner upon FceRI stimulation and that simultaneous inhibition of Akt and ERK resulted in nearly complete blockade of FceRI induced degranulation
Jang et al., BMC cancer 2010 (Breast Neoplasms...) : Inhibition of PI3K/Akt signaling by ANT2 shRNA caused down-regulation of membrane-type 1 matrix metalloproteinase ( MT1-MMP ) and vascular endothelial growth factor ( VEGF ) expression, decreased matrix metalloproteinase 2 (MMP2) and MMP9 activity, and suppressed migration and invasion of breast cancer cells ... Inhibition of PI3K/Akt signaling by ANT2 shRNA caused down-regulation of membrane-type 1 matrix metalloproteinase ( MT1-MMP ) and vascular endothelial growth factor ( VEGF ) expression, decreased matrix metalloproteinase 2 (MMP2) and MMP9 activity, and suppressed migration and invasion of breast cancer cells
Kramer et al., Eur J Immunol 2009 : The limiting effect of PI3K on TNF-alpha production from activated monocytes depended on the decrease of GSK-3beta activity, which significantly reduced the transactivation of NF-kappaB
Romano et al., J Cell Physiol 2006 : PI3K , ERK, JNK, and p38 kinases, known to mediate PDGF-BB signaling in the canonic dedifferentiative and proliferative response of smooth muscle cells ( SMC ) were rapidly activated by PDGF-BB but only p38 remained activated after 2-day stimulation
Uddin et al., Int J Cancer 2010 (Ovarian Neoplasms) : Cyclooxygenase-2 inhibition inhibits PI3K/AKT kinase activity in epithelial ovarian cancer
Wang et al., Shock 2005 (Endotoxemia...) : The enhanced MMP-9 levels induced by peptidoglycan was attenuated by inhibitors of p38 mitogen activated protein kinases ( MAPK ), ( SB202190, 25 microM ) and ERK1/2 ( PD98059, 25 microM ) and inhibitors of Src Tyrosine kinase ( PP2, 5 microM ) and PI3-K ( LY294002, 25 microM )
Mendez et al., Mol Cell Biol 1997 : The fact that PKC zeta stimulates general protein synthesis but not activation of p70S6K indicates that PKC zeta activation does not involve the proto-oncogene Akt , which is also activated by PI3K
Marzec et al., Oncogene 2007 (Lymphoma, T-Cell) : Accordingly, whereas the low-dose PI3K inhibitor wortmannin and Akt inhibitor III profoundly inhibited Akt phosphorylation, they had a very modest effect on S6rp and 4E-BP1 phosphorylation
Bailey et al., Mol Endocrinol 2004 : PI3K activates a downstream serine/threonine kinase , Akt ; therefore, we investigated the role of Akt in the interaction between PRL and TGF-beta signaling
Fan et al., J Neurosci 2010 : Intracerebroventricular E ( 2 ) infusion also increased PI3K phosphorylation after 15 min, and this effect was blocked by intrahippocampal PI3K, but not ERK , inhibition
Min et al., J Huazhong Univ Sci Technolog Med Sci 2012 : Our data showed that LY294002 and wortmannin, phosphatidylinositol 3-kinase (PI3K) and PI3K-like kinase inhibitors, increased Pim mRNA expression in ECs without altering the mRNA stability
Ghosh-Choudhury et al., J Biol Chem 2007 (MAP Kinase Signaling System) : Expression of dominant negative PI3K and PTEN, an inhibitor of PI3K signaling, significantly attenuated lovastatin induced transcription of BMP-2 ... Expression of dominant negative PI3K and PTEN , an inhibitor of PI3K signaling, significantly attenuated lovastatin induced transcription of BMP-2
Redondo et al., Mol Cell Endocrinol 2003 : GLP-1 , like insulin, activates PI3K/PKB , p70s6k, p44 and p42 MAP-kinase
Galve-Roperh et al., Mol Pharmacol 2002 (Astrocytoma) : In conclusion, CB(1) induced ERK activation was mediated by PI3K ( IB ) and this effect may have important consequences in the control of cell death/survival decision
Small et al., Proc Natl Acad Sci U S A 2010 : Similarly, we show that MRTF-A promotes PI3K/Akt signaling by up-regulating miR-486 expression
Le Guilloux et al., Rev Neurol (Paris) 2008 (Brain Neoplasms...) : High expression of the EGF receptor by tumor cells, activation of the PI3K/Akt and the Ras/Raf pathways represent interesting targets for new selective drugs under development
Kang et al., Eur J Pharmacol 2008 : In contrast, a JNK 1/2 inhibitor ( SP600125 ) and PI3K inhibitor ( wortmannin or LY294002 ) did not block the induction of IL-6 production by LPS
Aikawa et al., Circulation 2000 : Insulin strongly activated both PI3K and the putative downstream effector AKT : Moreover, a proapoptotic protein, BAD :, was significantly phosphorylated and inactivated by insulin through PI3K
Cheung et al., J Immunol 2008 (AIDS Dementia Complex) : Using pharmacological inhibitors and small interfering RNA gene knockdown, we demonstrated that concomitant activation of Lyn, Pyk2, and class IA PI3K are required for gp120 induced IL-1beta production
Li et al., Marine drugs 2013 : Furthermore, PI3K inhibitor LY294002 and Erk1/2 inhibitor U0126 suppressed the induction of HO-1 and translocation of Nrf-2 by xyloketal B, whereas P38 inhibitor SB203580 did not
Zheng et al., J Biol Chem 2000 (MAP Kinase Signaling System) : PMA also decreased IGF-1 induced tyrosine phosphorylation of insulin receptor substrate-1 and its association with PI3K
Marra et al., FEBS Lett 1995 : The results of this study show that in hepatic stellate cells PI 3-K is involved in ERK activation, although it is not necessary
Patsoukis et al., Cell cycle (Georgetown, Tex.) 2012 : The suppressive effect of PD-1 on Skp2 expression was mediated by inhibition of both PI3K/Akt and Ras/MEK/Erk pathways and was only partially reversed by IL-2, which restored activation of MEK/Erk but not Akt ... The suppressive effect of PD-1 on Skp2 expression was mediated by inhibition of both PI3K/Akt and Ras/MEK/Erk pathways and was only partially reversed by IL-2, which restored activation of MEK/Erk but not Akt
Wang et al., Clin Exp Allergy 2012 : The PI3K-specific inhibitor LY294002 also completely attenuated IL-25 induced bFGF expression
Hunzicker-Dunn et al., Proc Natl Acad Sci U S A 2012 : PI3K activation leads to activation of AKT through phosphorylation of AKT on Thr ( 308 ) and Ser ( 473 )
Zhu et al., Biochem Biophys Res Commun 2007 : PI3K is negatively regulated by PIK3IP1 , a novel p110 interacting protein ... PI3K is negatively regulated by PIK3IP1 , a novel p110 interacting protein
Zhuang et al., American journal of physiology. Renal physiology 2004 : Inhibition of PI3K with LY-294002 blocked Akt phosphorylation and proliferation, whereas U-0126 blocked ERK1/2 phosphorylation but had no effect on proliferation ... Inhibition of PI3K with LY-294002 blocked Akt phosphorylation and proliferation, whereas U-0126 blocked ERK1/2 phosphorylation but had no effect on proliferation ... These results show that EGFR activation is required for RPTC proliferation and migration and that proliferation is mediated by PI3K , whereas migration is mediated by p38
Lee et al., Am J Physiol Heart Circ Physiol 2006 (Inflammation...) : The PI3K inhibitor and a dominant negative mutant of Akt suppressed Akt and eNOS phosphorylation and NO production ... The PI3K inhibitor and a dominant negative mutant of Akt suppressed Akt and eNOS phosphorylation and NO production
Chang et al., Mol Pharmacol 2004 : In this study, we explore the role of Ras, phosphoinositide-3-OH-kinase (PI3K) , Akt, and transcription factor nuclear factor-kappaB (NF-kappaB) in YC-1 induced COX-2 expression in A549 cells ... A Ras inhibitor ( manumycin A ), a PI3K inhibitor ( wortmannin ), an Akt inhibitor ( 1l-6-Hydroxymethyl-chiro-inositol2- [ ( R ) -2-O-methyl-3-O-octadecylcarbonate ] ), and an NF-kappaB inhibitor [ pyrrolidine dithiocarbamate ( PDTC ) ] all reduced YC-1 induced COX-2 expression ... Taken together, these results indicate that YC-1 might activate the sGC/cGMP/PKG pathway to induce Ras and PI3K/Akt activation, which in turn initiates IKKalpha/beta and NF-kappaB activation and finally induces COX-2 expression in A549 cells
Chen et al., J Biol Chem 2011 : The PI3K dependent signaling kinase complex mTORC2 ( mammalian target of rapamycin complex 2 ) has been defined as the regulatory Ser-473 kinase of Akt
Lakhanpal et al., Blood 2010 (Leukemia, Erythroblastic, Acute) : In addition, RNA interference ( RNAi ) -mediated suppression of SHIP-1 in erythroleukemia cells activates the phosphatidylinositol 3-kinase (PI 3-K) and extracellular signal regulated kinase/mitogen activated protein kinase ( ERK/MAPK ) pathways, blocks erythroid differentiation, accelerates erythropoietin induced proliferation, and leads to PI 3-K dependent Fli-1 up-regulation
Chiron et al., Cell cycle (Georgetown, Tex.) 2013 (Lymphoma) : PIK3IP1 , a negative PI3K regulator , appears to mediate pG1 sensitization to PI3K inhibition ; it is markedly reduced in MCL tumor cells compared with normal peripheral B cells, profoundly induced in pG1 and required for pG1 sensitization to GS-1101 ... PIK3IP1 , a negative PI3K regulator , appears to mediate pG1 sensitization to PI3K inhibition ; it is markedly reduced in MCL tumor cells compared with normal peripheral B cells, profoundly induced in pG1 and required for pG1 sensitization to GS-1101
Chiou et al., Eur J Pharmacol 2011 : Psoralidin inhibits LPS induced iNOS expression via repressing Syk mediated activation of PI3K-IKK-I?B signaling pathways
Liu et al., PloS one 2007 : We showed that H ( 2 ) O ( 2 ) induces rCMECs apoptosis mainly through the PI3K/ERK pathway, since a PI3K inhibitor ( LY294002 ) blocked ERK activation caused by H ( 2 ) O ( 2 ) and a specific inhibitor of MEK ( U0126 ) protected cells from apoptosis
Hennenlotter et al., Oncol Rep 2008 (Carcinoma, Renal Cell...) : We concluded that a strong expression of PTEN in renal cell cancer did not block the PI3K mediated phosphorylation of Akt in the tumour specimens analysed
Cao et al., Biochem J 2006 : MEK [ MAPK ( mitogen activated protein kinase ) /ERK ( extracellular-signal regulated kinase ) kinase ] /ERK inhibitor U0126 and PI3K ( phosphoinositide 3-kinase ) inhibitor LY294002 also inhibited EGF induced AQP3 expression and cell migration
Kolb et al., Int J Cancer 2007 (Pancreatic Neoplasms) : HRGs induced phosphorylation of different ErbB receptors as well as activation of MAPK, p38MAPK, JNK and PI3K in a cell- and ligand-specific manner
Byles et al., International journal of biological sciences 2010 : Aberrant cytoplasm localization and protein stability of SIRT1 is regulated by PI3K/IGF-1R signaling in human cancer cells ... We found this predominant cytoplasmic localization of SIRT1 is regulated by elevated mitotic activity and PI3K/IGF-1R signaling in cancer cells ... We show that aberrant cytoplasmic localization of SIRT1 is due to increased protein stability and is regulated by PI3K/IGF-1R signaling ... Our study represents the first identification that aberrant cytoplasm localization is one of the specific alternations to SIRT1 that occur in cancer cells, and PI3K/IGF-1R signaling plays an important role in the regulation of cytoplasmic SIRT1 stability
Yang et al., Eur J Pharmacol 2010 : Plumbagin stimulated ERK1/2 activity was attenuated by the MEK1/2 inhibitor PD98059 and Ras inhibitor manumycin A, whereas plumbagin stimulated Akt activity was blocked by the PI3K inhibitor LY294002 ... These results suggest that plumbagin activates NAD ( P ) H oxidase , Src, and PI3K, and that the activated PI3K or PDK1 subsequently stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells ... These results suggest that plumbagin activates NAD ( P ) H oxidase, Src, and PI3K, and that the activated PI3K or PDK1 subsequently stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells ... These results suggest that plumbagin activates NAD ( P ) H oxidase, Src, and PI3K, and that the activated PI3K or PDK1 subsequently stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells ... These results suggest that plumbagin activates NAD ( P ) H oxidase, Src, and PI3K, and that the activated PI3K or PDK1 subsequently stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells ... These results suggest that plumbagin activates NAD ( P ) H oxidase, Src, and PI3K, and that the activated PI3K or PDK1 subsequently stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells
Wang et al., Lipids in health and disease 2012 (Obesity) : HDL and apoA-I could activate PI3K-Akt-mTORC1 signaling which negatively regulates autophagy
Buxadé et al., Immunology 2001 : The increase in translation of TNF-alpha due to serum could be inhibited by the phosphatidylinositol (PI) 3-K inhibitors, wortmannin and LY294002, suggesting that PI 3-K is involved in the translational control of TNF-alpha by serum
Chakravarti et al., Cancer Res 2002 (Brain Neoplasms...) : It was found that BCNU inhibited radiation induced apoptosis through EGFR mediated activation of PI3-K/AKT via RAS
Goncharova et al., Am J Physiol Lung Cell Mol Physiol 2002 : In parallel experiments, stimulation of human PVSM cells with PDGF induced PI3K dependent activation of Akt, p70 S6 kinase, and ribosomal protein S6 but not mitogen activated protein kinase ... In parallel experiments, stimulation of human PVSM cells with PDGF induced PI3K dependent activation of Akt , p70 S6 kinase, and ribosomal protein S6 but not mitogen activated protein kinase
Morrison et al., J Virol 2003 : LMP2A activated Akt in a PI3K dependent manner, and the downstream Akt targets glycogen synthase kinase 3beta ( GSK3beta ) and the Forkhead transcription factor FKHR were phosphorylated and inactivated in LMP2A expressing HFK cells ... LMP2A activated Akt in a PI3K dependent manner, and the downstream Akt targets glycogen synthase kinase 3beta ( GSK3beta ) and the Forkhead transcription factor FKHR were phosphorylated and inactivated in LMP2A expressing HFK cells ... LMP2A activated Akt in a PI3K dependent manner, and the downstream Akt targets glycogen synthase kinase 3beta ( GSK3beta ) and the Forkhead transcription factor FKHR were phosphorylated and inactivated in LMP2A expressing HFK cells ... The cytoplasmic accumulation of beta-catenin downstream of LMP2A was independent of PI3K signaling, whereas its nuclear translocation was dependent on PI3K signaling
Yu et al., J Immunol 2006 : Such effect of inhibiting PI3K with wortmannin was mimicked by the PI3K inhibitor LY294002, and, conversely, a constitutively active PI3K prevented p38 activation in response to flagellin
Caravatta et al., J Cell Physiol 2008 (Leukemia...) : PI3-K/Akt dependent activation of cAMP-response element binding ( CREB ) protein in Jurkat T leukemia cells treated with TRAIL
Vlotides et al., Mol Endocrinol 2006 (Pituitary Neoplasms) : Phosphatidylinositol 3 kinase , protein kinase C, and MAPK, but not c-Jun N-terminal kinase and Janus activating kinase signaling regulated EGF induced Pttg1, as well as proliferating cell nuclear antigen mRNA expression and entry into S-phase ... Phosphatidylinositol 3 kinase , protein kinase C, and MAPK, but not c-Jun N-terminal kinase and Janus activating kinase signaling regulated EGF induced Pttg1 , as well as proliferating cell nuclear antigen mRNA expression and entry into S-phase
Kim et al., Carcinogenesis 2006 (Prostatic Neoplasms) : Importantly, the degree of Akt and PI3K phosphorylations induced by EGF were also significantly suppressed
Kenzel et al., J Immunol 2012 (Inflammation...) : We found that GBS induced, MyD88 dependent chemokine formation of PML was specifically downmodulated by insulin via insulin receptor mediated induction of PI3K
Chen et al., Am J Physiol Lung Cell Mol Physiol 2013 (Anoxia) : Thus, our findings indicate that Trx1 contributes to hypoxia induced PASMC proliferation by modulating HIF activation and subsequent PI3K-Akt activation
St-Germain et al., Molecular cancer 2004 (Endometrial Neoplasms) : Inhibition of PI 3-K with Wortmannin and LY294002 blocked IkappaB phosphorylation, reduced NF-kappaB nuclear activity, reduced COX-2 expression and induced apoptosis ... Inhibition of PI 3-K with Wortmannin and LY294002 blocked IkappaB phosphorylation, reduced NF-kappaB nuclear activity, reduced COX-2 expression and induced apoptosis
Ning et al., Arch Toxicol 2013 (Airway Remodeling...) : PDGF induced DNA methyltransferase 1 (DNMT1) expression was mediated by activation of PI3K/Akt and ERK signaling in RASM cells
Liu et al., Mol Immunol 2008 : These results suggest that NAC activates mainly PI3K to phosphorylate p65 and subsequently induces DNA binding activity of NF-kappaB, independent of its antioxidative function
Hwang et al., Toxicol Appl Pharmacol 2008 : Mechanism of phytoestrogen puerarin mediated cytoprotection following oxidative injury : estrogen receptor dependent up-regulation of PI3K/Akt and HO-1 ... Also, puerarin mediated increases in PI3K activation and HO-1 induction were reversed by co-treatment with ICI 182,780 and pertussis toxin
Dai et al., Biochem Biophys Res Commun 2002 : SGKL is activated via its lipid binding domain ( phox homology domain ) in response to PI3K signaling
Ferretti et al., Leukemia 2011 (Leukemia, Lymphocytic, Chronic, B-Cell) : CX(3)CL1 induced phosphorylation of PI3K , Erk1/2, p38, Akt and Src was involved in induction of CLL chemotaxis
Xia et al., J Biol Chem 2007 (Neoplasms) : Collectively, these findings demonstrate that p21 ( RAS ), through its downstream effector PI3K , induces PKCdelta expression and that this increase in PKCdelta activity, acting through Akt, is required for cell survival
Sanderson et al., J Neurochem 2008 (Brain Ischemia...) : We hypothesized that insulin acts through PI3-K , Akt, and Bcl-2 family proteins to inhibit cytochrome c release following transient global brain ischemia
Andò et al., Mol Cell Endocrinol 2005 : Interestingly, in the presence of a disruptor of insulin signaling wortmannin, an inhibitor of PI3K , the intrinsic activity of G6PDH drops
Xiao et al., Virology 2009 : The PI3K-speci fi c inhibitor, LY294002, suppressed Akt phosphorylation in a dose dependent manner, suggesting that AcMNPV induced Akt phosphorylation is PI3K dependent
Delcommenne et al., Proc Natl Acad Sci U S A 1998 : We also demonstrate here that ILK is an upstream effector of the Pi(3)K dependent regulation of both protein kinase B ( PKB/AKT ) and glycogen synthase kinase 3 ( GSK-3 ) ... We also demonstrate here that ILK is an upstream effector of the Pi(3)K dependent regulation of both protein kinase B ( PKB/AKT ) and glycogen synthase kinase 3 ( GSK-3 ) ... ILK is thus a receptor-proximal effector for the Pi(3)K dependent , extracellular matrix and growth factor mediated, activation of PKB/AKT , and inhibition of GSK-3 ... ILK is thus a receptor-proximal effector for the Pi(3)K dependent , extracellular matrix and growth factor mediated, activation of PKB/AKT , and inhibition of GSK-3
Nizhynska et al., Dev Neurobiol 2007 : Moreover, agrin induced activation of Rac and Cdc42 is impaired in the presence of PI3-K inhibitors ... Moreover, agrin induced activation of Rac and Cdc42 is impaired in the presence of PI3-K inhibitors
Hetman et al., J Biol Chem 2002 : Here, we report that inhibition of ERK1/2 increased the basal activity of GSK3beta in cortical neurons and that both ERK1/2 and PI3K were required for brain derived neurotrophic factor (BDNF) suppression of GSK3beta activity ... Here, we report that inhibition of ERK1/2 increased the basal activity of GSK3beta in cortical neurons and that both ERK1/2 and PI3K were required for brain derived neurotrophic factor (BDNF) suppression of GSK3beta activity ... Here, we report that inhibition of ERK1/2 increased the basal activity of GSK3beta in cortical neurons and that both ERK1/2 and PI3K were required for brain derived neurotrophic factor (BDNF) suppression of GSK3beta activity ... Activation of both endogenous ERK1/2 and PI3K signaling pathways was required for BDNF to block apoptosis induced by expression of recombinant GSK3beta ... Although both PI3K and ERK1/2 inhibited GSK3beta activity, neither had an effect on GSK3beta phosphorylation at Tyr-216 ... Interestingly, PI3K ( but not ERK1/2 ) induced the inhibitory phosphorylation of GSK3beta at Ser-9
Sharma et al., J Biol Chem 2002 (Prostatic Neoplasms) : Phosphatidylinositol 3-kinase/Akt stimulates androgen pathway through GSK3beta inhibition and nuclear beta-catenin accumulation
Kristof et al., J Pharmacol Exp Ther 2005 (Prostatic Neoplasms) : In human lung epithelial adenocarcinoma ( A549 ) cells, LY303511, like rapamycin, inhibited mTOR dependent phosphorylation of S6K, but not PI3K dependent phosphorylation of Akt
Pola et al., J Biol Chem 2003 (MAP Kinase Signaling System...) : Although somatostatin does not affect PI3-K , it inhibits ERK 1/2 and the small G-protein Rac activation and ruffle formation induced by PDGF
Kim et al., J Clin Immunol 2012 (Acute Lung Injury) : Additionally, infiltration of dendritic cells (DCs) and expression of toll-like receptor 4 (TLR4) were significantly increased in the lung of LPS treated mice, and inhibition of PI3K-? reduced the infiltration of DCs and TLR4 expression in the lung
Weidenaar et al., Mol Cancer Res 2013 (Leukemia, Myeloid, Acute...) : Proteome profiler array analysis of downstream kinases revealed that PTK/ZK reduced activation of PI3K/Akt kinase signaling
Yano et al., Cell communication and signaling : CCS 2012 : PI3K/Akt dependent activation of cAMP responsive element binding protein ( CREB)-1 induced expression of matrix metalloproteinase (MMP)-9 and suppressing MMP activity by doxycycline partially reversed FN accumulation in the InsR silenced cells ... PI3K/Akt dependent activation of cAMP responsive element binding protein ( CREB)-1 induced expression of matrix metalloproteinase (MMP)-9 and suppressing MMP activity by doxycycline partially reversed FN accumulation in the InsR silenced cells ... PI3K/Akt dependent activation of cAMP responsive element binding protein ( CREB)-1 induced expression of matrix metalloproteinase (MMP)-9 and suppressing MMP activity by doxycycline partially reversed FN accumulation in the InsR silenced cells
Dufourny et al., J Endocrinol 2000 (Breast Neoplasms) : Since transcriptional activation of the cyclin D1 promoter by EGF, E2 and TPA is independent of PI3-K activity, these findings suggest a post-transcriptional role for PI3-K in the regulation of cyclin D1 expression ... Since transcriptional activation of the cyclin D1 promoter by EGF , E2 and TPA is independent of PI3-K activity, these findings suggest a post-transcriptional role for PI3-K in the regulation of cyclin D1 expression
Wang et al., Biochemistry 2006 : Here we demonstrate that PAR-2 can increase PI3K activity through a Galphaq/Ca ( 2+ ) -dependent pathway involving PYK2 and a Src-family kinase, while inhibiting PI3K activity through a beta-arrestin dependent mechanism, and that beta-arrestin-1 can directly associate with and inhibit the catalytic activity of p110alpha ... Here we demonstrate that PAR-2 can increase PI3K activity through a Galphaq/Ca ( 2+ ) -dependent pathway involving PYK2 and a Src-family kinase, while inhibiting PI3K activity through a beta-arrestin dependent mechanism, and that beta-arrestin-1 can directly associate with and inhibit the catalytic activity of p110alpha
Kim et al., Exp Mol Med 2008 (Ascites...) : Both LPA- and AOCP induced MEF cell migration was completely attenuated by PI3K inhibitor, LY294002
Heffron et al., Bioorg Med Chem Lett 2010 : Identification of GNE-477 , a potent and efficacious dual PI3K/mTOR inhibitor ... This discovery led to the identification of GNE-477 ( 8 ), a potent and efficacious dual PI3K/mTOR inhibitor
Lin et al., J Cell Physiol 2007 (Lung Neoplasms) : We initially observed that IL-1beta induced ICAM-1 promoter activity was attenuated by the inhibitors of Src ( PP1 ), PDGFR ( AG1296 ), PI3-K ( LY294002 and wortmannin ), and Akt ( SH-5 ), revealed by reporter gene assay, Western blotting, and RT-PCR analyses ... The involvement of Src and PI3-K/Akt in IL-1beta induced ICAM-1 expression was significantly attenuated by transfection of A549 cells with dominant negative plasmids of Src, p85 and Akt, respectively ... Src, PDGFR, and PI3K/Akt mediated the effects of IL-1beta because pretreatment with PP1, AG1296, and wortmannin also abrogated IL-1beta stimulated Src, PDGFR, and Akt phosphorylation, respectively
Mukherjee et al., Proc Natl Acad Sci U S A 2013 : The innate immune receptor, Toll-like receptor 2/6 heterodimer, is exploited by Sb ( R ) LD to activate ERK and nuclear translocation of NF-?B involving p50/c-Rel leading to IL-10 induction, whereas MDR1 up-regulation is mediated by PI3K/Akt and the JNK pathway
Radhakrishnan et al., J Immunol 2007 : Analysis of human or mouse DC treated with the B7-DC cross linking Ab revealed PI3K dependent phosphorylation of AKT accompanied by mobilization of NF-kappaB
Ko et al., Immunobiology 2011 : The suppression of Fgr with PP2 and Fgr siRNA inhibited activating phosphorylation of PKCd and PI3K/Akt , but not IL-1 receptor associated kinase (IRAK)1, a well-known MyD88 dependent signaling molecule, and Erk1/2, p38, and JNK ... Rottlerin and PKCd siRNA also inhibited expression of IL-32a and activation of PI3K/Akt , but not of IRAK1 and mitogen activation protein ( MAP ) kinases
Petrella et al., Cancer Biol Ther 2009 (Carcinoma, Renal Cell...) : Activation of PI3K/Akt/mTOR leads to increased HIF-1alpha expression in certain cancer cells, supporting the rationale of using mTOR inhibitors as anti-cancer agents
Carini et al., Gastroenterology 2004 (Reperfusion Injury) : PI3K activation in preconditioned hepatocytes required the stimulation of adenosine A 2A receptors and was mimicked by adenosine A 2A receptors agonist CGS21680 ... In the cells treated with CGS21680, PI3K activation was prevented either by inhibiting adenylate cyclase and PKA with, respectively, 2,5-dideoxyadenosine and H89 or by blocking Galphai-protein and Src tyrosine kinase with, respectively, pertussis toxin and PP2 ... In the cells treated with CGS21680, PI3K activation was prevented either by inhibiting adenylate cyclase and PKA with, respectively, 2,5-dideoxyadenosine and H89 or by blocking Galphai-protein and Src tyrosine kinase with, respectively, pertussis toxin and PP2 ... This suggested that PKA phosphorylated adenosine A 2A receptors may activate PI3K by coupling it with Galphai-protein through Src ... PI3K is activated following hepatocyte hypoxic preconditioning by the combined stimulation of adenosine A 2A receptors, PKA, Galphai protein, and Src ... PI3K is activated following hepatocyte hypoxic preconditioning by the combined stimulation of adenosine A 2A receptors, PKA, Galphai protein, and Src ... PI3K is activated following hepatocyte hypoxic preconditioning by the combined stimulation of adenosine A 2A receptors, PKA , Galphai protein, and Src
Gupta et al., Lung 2004 (Carcinoma, Non-Small-Cell Lung...) : Pharmacologically inhibiting PI3K led to decreased Akt phosphorylation and radio sensitization of all three cell lines
Gonzalez-Robayna et al., Mol Endocrinol 2000 : Lastly, FSH mediated phosphorylation of p38MAPK is negatively affected by A kinase and PI3-K , suggesting that it may be downstream of specific members of the cAMP-GEF/Rap/Raf pathway ... Lastly, FSH mediated phosphorylation of p38MAPK is negatively affected by A kinase and PI3-K , suggesting that it may be downstream of specific members of the cAMP-GEF/Rap/Raf pathway
Almeida et al., Cell Death Differ 2005 (MAP Kinase Signaling System) : The results indicate that the protective effect of BDNF in hippocampal neurons against glutamate toxicity is mediated by the PI3-K and the Ras/MAPK signaling pathways, and involves a long-term change in protein synthesis
McMullen et al., Proc Natl Acad Sci U S A 2007 (Cardiomyopathy, Dilated...) : PI3K ( p110alpha ) signaling negatively regulated G protein coupled receptor stimulated extracellular responsive kinase and Akt ( via PI3K, p110gamma ) activation in isolated cardiomyocytes ... PI3K ( p110alpha ) signaling negatively regulated G protein coupled receptor stimulated extracellular responsive kinase and Akt ( via PI3K, p110gamma ) activation in isolated cardiomyocytes
Westra et al., Ann N Y Acad Sci 2007 (Arthritis, Rheumatoid) : The effect of inhibitors of the PI3K and the ERK pathway on HIF-1alpha protein expression was measured ... Furthermore, it was shown that cytokine induced mRNA expression of HIF-1alpha was inhibited by the PI3K inhibitor
Parikh et al., J Immunol 2004 : Because inhibition of PI3K or its target Akt also triggers mitochondrial translocation of Bax in T cells and apoptosis in Bax transfected cell lines, we generated Bax deletion mutants to identify the region ( s ) that confers sensitivity to regulation by MEK1 and Akt
Bando et al., Exp Eye Res 2006 (MAP Kinase Signaling System...) : We performed the gel contraction assay to evaluate the effect of PDGF in cultured ARPE-19 cells under the presence or absence of PD98059, MAPK inhibitor or wortmannin, PI3K inhibitor
Rudd et al., Immunol Rev 2009 : We also present recent findings on T-cell receptor interacting molecule ( TRIM ) regulation of CTLA-4 surface expression, and a signaling pathway involving CTLA-4 activation of PI3K and protein kinase B (PKB)/AKT by which cell survival is ensured under conditions of anergy induction
Hsieh et al., J Rheumatol 2007 (Osteoarthritis, Knee) : Enzyme linked immunosorbent assay and gelatin zymography were used to investigate the effects of ERK 1/2 inhibitor U0126, JNK and p38 inhibitor SB203580, and PI3K inhibitor LY294002 on the secretion of u-PA, PAI-1, MMP-2 , and MMP-9 in early osteoarthritic tissue cultures, with or without interleukin 1alpha (IL-1alpha) and lipopolysaccharide (LPS) induction ... Enzyme linked immunosorbent assay and gelatin zymography were used to investigate the effects of ERK 1/2 inhibitor U0126, JNK and p38 inhibitor SB203580, and PI3K inhibitor LY294002 on the secretion of u-PA, PAI-1, MMP-2, and MMP-9 in early osteoarthritic tissue cultures, with or without interleukin 1alpha (IL-1alpha) and lipopolysaccharide (LPS) induction ... Enzyme linked immunosorbent assay and gelatin zymography were used to investigate the effects of ERK 1/2 inhibitor U0126, JNK and p38 inhibitor SB203580, and PI3K inhibitor LY294002 on the secretion of u-PA, PAI-1 , MMP-2, and MMP-9 in early osteoarthritic tissue cultures, with or without interleukin 1alpha (IL-1alpha) and lipopolysaccharide (LPS) induction
Bertrand et al., Leukemia 2005 : However, only inhibition of both PI3K and MEK or both mTOR and MEK resulted in a dramatic increase in the number of annexinV ( + ) /PI ( + ) apoptotic events within a 24 h period
Villarreal et al., Mol Cell Biochem 2006 : These results suggest that Ins and Ang II modulate IRS-4 tyr-phosphorylation in a PI3K dependent manner
Chang et al., Biochem J 2009 (Ovarian Neoplasms) : The anti-apoptotic ability was suppressed and the expression of Bcl-2 family in response to mesothelin was altered by inhibiting PI3K activity, but not by inhibiting MAPK activity ... Thus mesothelin can inhibit paclitaxel induced cell death mainly by involving PI3K signalling in the regulation of Bcl-2 family expression
Ortiz et al., American journal of physiology. Renal physiology 2004 : In other cells, eNOS activation by shear stress is mediated by phosphatidylinositol 3-OH kinase (PI3)-kinase
Liu et al., Acta Biochim Biophys Sin (Shanghai) 2010 : Results showed that apelin-13 promoted the expression of phospho-PI3K and phospho-Akt in dose- and timedependent manner ... PI3K inhibitor LY294002 significantly decreased the expression of phospho-PI3K , phospho-Akt, phospho-ERK1/2, and cyclin D1 induced by apelin-13
Kim et al., Biol Pharm Bull 2007 : Water extract of Korean red ginseng stimulates angiogenesis by activating the PI3K/Akt dependent ERK1/2 and eNOS pathways in human umbilical vein endothelial cells ... Water extract of Korean red ginseng stimulates angiogenesis by activating the PI3K/Akt dependent ERK1/2 and eNOS pathways in human umbilical vein endothelial cells ... Inhibition of PI3K activity by wortmannin completely inhibited KRGE induced angiogenesis and phosphorylation of Akt, ERK1/2 , and eNOS, indicating that PI3K/Akt activation is an upstream event of the KRGE mediated angiogenic pathway ... Inhibition of PI3K activity by wortmannin completely inhibited KRGE induced angiogenesis and phosphorylation of Akt , ERK1/2, and eNOS, indicating that PI3K/Akt activation is an upstream event of the KRGE mediated angiogenic pathway ... Inhibition of PI3K activity by wortmannin completely inhibited KRGE induced angiogenesis and phosphorylation of Akt, ERK1/2, and eNOS , indicating that PI3K/Akt activation is an upstream event of the KRGE mediated angiogenic pathway ... This study demonstrated that KRGE stimulates in vitro and in vivo angiogenesis through the activation of the PI3K/Akt dependent ERK1/2 and eNOS signal pathways and their cross talk ... This study demonstrated that KRGE stimulates in vitro and in vivo angiogenesis through the activation of the PI3K/Akt dependent ERK1/2 and eNOS signal pathways and their cross talk
Yart et al., J Biol Chem 2002 : Indeed, both membrane redistribution and phosphorylation of Gab1 were reduced in the presence of PI3K inhibitors or dominant negative p110beta
Lindvall et al., Biochem Biophys Res Commun 2002 : In addition, we have investigated the involvement of PI 3-K in the MAPKs and ERK and JNK phosphorylation, in the presence or absence of Btk ... In addition, we have investigated the involvement of PI 3-K in the MAPKs and ERK and JNK phosphorylation, in the presence or absence of Btk
Fung et al., Cell Signal 2003 : IL-2 activation of a PI3K dependent STAT3 serine phosphorylation pathway in primary human T cells ... However, the coupling of STAT3 serine phosphorylation to PI3K in response to IL-2 has yet to be shown in either T cell lines or primary human T cells
Choi et al., Int Immunopharmacol 2006 : However, many aspects of the PI3K negative regulation of NF-kappaB activation remain to be clarified ... The present study was conducted to shed light on cell-type specificity, stimulus specificity, and upstream mechanisms of the enhanced NF-kappaB activation by PI3K inhibitors
Wu et al., Oncogene 2001 (Glioblastoma) : Based on these results, we conclude that SHP-2 is required for mediating PI3K/Akt activation, and the N-terminal SH2 domain is critically important for a `` positive '' role of SHP-2 in regulating PI3K pathway activation
Gulati et al., Biochem Soc Trans 2007 : Nutrient overload induces constitutive S6K1 ( S6 kinase 1 ) activation, which leads to insulin resistance by suppressing insulin induced class I PI3K ( phosphoinositide 3-kinase ) signalling [ Um, Frigerio, Watanabe, Picard, Joaquin, Sticker, Fumagalli, Allegrini, Kozma, Auwerx and Thomas ( 2004 ) Nature 431, 200-205 ]
Hideshima et al., Oncogene 2001 (MAP Kinase Signaling System...) : We demonstrate that Dex induced apoptosis in MM.1S cells is mediated by downstream activation of caspase-9 , with resultant caspase-3 cleavage ; and conversely, that IL-6 triggers activation of PI3-K and its association with SHP2, inactivates caspase-9, and protects against Dex induced apoptosis ... We demonstrate that Dex induced apoptosis in MM.1S cells is mediated by downstream activation of caspase-9, with resultant caspase-3 cleavage ; and conversely, that IL-6 triggers activation of PI3-K and its association with SHP2, inactivates caspase-9, and protects against Dex induced apoptosis ... Finally, we show that IL-6 triggered PI3-K/Akt signaling in MM.1S cells inactivates forkhead transcriptional factor (FKHR), with related G1/S phase transition, whereas LY294002 blocks this signaling, resulting in upregulation of p27 ( KIP1 ) and G1 growth arrest
Riley et al., Oncogene 2001 : This was because reduced PI3-K activity lead to proteolytic degradation of p27
Zundel et al., Genes Dev 1998 : Ceramide inhibition of PI(3)K is dependent on acid-sphingomyelinase
Ling et al., J Cell Biochem 1999 (Astrocytoma) : Our data suggest a role for PI3-K and p125FAK complex formation in PI3-K activation
Yang et al., Oxidative medicine and cellular longevity 2013 : In conclusion, these results suggest that SPD induces PI3K/Akt-Nrf2 mediated HO-1 expression in human endothelial cells, which may have a role in cytoprotection of the cells against oxidative stress induced death ... In conclusion, these results suggest that SPD induces PI3K/Akt-Nrf2 mediated HO-1 expression in human endothelial cells, which may have a role in cytoprotection of the cells against oxidative stress induced death
Iskandar et al., Am J Physiol Endocrinol Metab 2010 (Obesity) : Both insulin and leptin signaling converge on phosphatidylinositol 3-OH kinase [PI(3)K ] /3-phosphoinositide dependent protein kinase-1 ( PDK-1 ) /protein kinase B ( PKB, also known as Akt ) in proopiomelanocortin (POMC) neurons
Gerling et al., Neurochem Int 2004 : Accordingly, inhibition of PI3-K by wortmannin and MAPK-kinase ( MEK ) inhibition by UO126 abolished the neuroprotective effects
Donahue et al., Eur J Immunol 2007 : We used flow cytometry and magnetic cell sorting to examine the requirement for PI3K and mTOR in responses of splenic B cell subsets to BCR and LPS stimulation ... We used flow cytometry and magnetic cell sorting to examine the requirement for PI3K and mTOR in responses of splenic B cell subsets to BCR and LPS stimulation
Condorelli et al., Proc Natl Acad Sci U S A 2002 (Cardiomyopathy, Hypertrophic) : In fact, both IGF-1 and IL6-like cytokines induce hypertrophic and antiapoptotic signals in cardiomyocytes through PI3K dependent Akt activation
Senbonmatsu et al., EMBO J 2003 (Cardiomegaly) : AT(2) enhances expression of p85 alpha PI3K followed by enhanced p70 ( S6 ) kinase, essential to protein synthesis
Wang et al., Cell Death Differ 2006 : RA/BDNF treatment activates Cdk5 mediated PI3K/Akt and ERK pathways ... Inhibition of Cdk5 inhibits PI3K/Akt and ERK phosphorylation and Bcl-2 expression, and thus sensitizes the differentiated cells to DNA-damage
Blando et al., Am J Pathol 2009 (Adenocarcinoma...) : Lesions in both Pten ( +/- ) ; Tsc2 ( +/- ), and Pten ( +/- ) mice exhibited loss of PTEN expression and activation of PI3K signaling
Yang et al., Biosci Rep 2012 (Insulin Resistance) : In contrast, saturated fatty acid exposure caused insulin resistance, reducing PI3K ( phosphoinositide 3-kinase ) and ERK ( extracellular-signal regulated kinase ) activation while increasing activation of stress kinases JNK ( c-Jun N-terminal kinase ) and p38
Kim et al., J Biol Chem 2004 : Down-regulation of PTEN by NIK/NF-kappaB results in activation of the PI3K/Akt pathway and augmentation of TNF-alpha induced PI3K/Akt stimulation
Peyrollier et al., Biochem J 2000 : PI3K activation was inhibited by wortmannin and was not dependent upon insulin receptor substrate-1 activation
Slomiany et al., J Pharmacol Exp Ther 2006 (Anoxia) : IGF-1 stimulated VEGF promoter activity was phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR ( mammalian target of rapamycin ) -dependent, whereas VEGF secretion was only partially reduced by inhibition of PI3K/Akt/mTOR and HIF-1 activities
Osawa et al., J Immunol 2001 : TNF-alpha induced activations of PI3K and Akt were inhibited by DMS
Simoncini et al., Arterioscler Thromb Vasc Biol 2003 : By using cultured human saphenous vain endothelial cells, we found that cell membrane bound ERalpha colocalizes with PI3K and may be responsible for PI3K activation ... Furthermore, we characterized the subsequent steps in the activation of the PI3K/Akt signaling cascade, comparing the molecular events that follow insulin or estradiol activation of PI3K
Brunelli et al., J Steroid Biochem Mol Biol 2009 (Breast Neoplasms) : These findings suggest that inhibition of PI(3)K is a novel mechanism which contributes to 8PN activity to inhibit cancer cell survival and EGF induced proliferation
Kim et al., J Pharmacol Exp Ther 2006 : These results show that PI3K/Akt/p70S6K signaling is active in the insulin mediated up-regulation of the antioxidant defense system and that low insulin levels, as encountered in diabetes, potentially increase the susceptibility of hepatocytes to xenobiotic mediated and/or oxidative stress mediated damage
Simonsen et al., Nature 1998 : The association of EEA1 with the endosomal membrane requires Rab5-GTP and PI(3)K activity, and excess Rab5-GTP stabilizes the membrane association of EEA1 even when PI(3)K is inhibited ... The association of EEA1 with the endosomal membrane requires Rab5-GTP and PI(3)K activity, and excess Rab5-GTP stabilizes the membrane association of EEA1 even when PI(3)K is inhibited
Mehrhof et al., Circulation 2001 : IGF-I-stimulation was followed by a PI3K dependent phosphorylation of AKT and BAD and an MEK1 dependent phosphorylation of extracellular signal regulated kinase ( ERK ) 1 and ERK2 ... IGF-I-stimulation was followed by a PI3K dependent phosphorylation of AKT and BAD and an MEK1 dependent phosphorylation of extracellular signal regulated kinase ( ERK ) 1 and ERK2 ... In summary, our data demonstrate that in cardiomyocytes, the antiapoptotic effect of IGF-I requires both PI3K- and MEK1 dependent pathways leading to the activation of the transcription factor CREB, which then induces the expression of the antiapoptotic factor bcl-2
Rotshenker et al., Glia 2008 (Demyelinating Diseases...) : S-trans, trans-farnesylthiosalicylic (FTS), which inhibits Galectin-3/MAC-2 dependent activation of PI3K through Ras, inhibited phagocytosis ... A role for Galectin-3/MAC-2 dependent activation of PI3K through Ras, mostly K-Ras, is thus suggested
Nagel et al., Leukemia 2005 (Hodgkin Disease) : HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3 ... HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3
Xuan Nguyen et al., Biochem Biophys Res Commun 2006 (Prostatic Neoplasms) : Employing the PI3K inhibitor and a variety of mutants PI3K, we showed that nuclear translocation of Akt was mediated by activation of PI3K , and Akt phosphorylation status in the nucleus required PI3K activity ... Thus the activity of PI3K might contribute to the nuclear translocation of Akt , and that Akt phosphorylation is essential for its nuclear retention under NGF stimulation conditions
Kuo et al., J Cell Physiol 2006 (MAP Kinase Signaling System) : Our data showed that extracellular signal regulated kinase ( ERK ) pathway inhibitor PD98059, but not the c-Jun N-terminal kinase (JNK) inhibitor SP600125, p38 kinase inhibitor SB203580, and PI3K inhibitor wortmannin, attenuated Src induced MMP-2 promoter activity
Inabe et al., J Exp Med 2002 : The functional interaction between PI3K and Rac1 was also demonstrated by increased PI3K activity in the presence of GTP bound Rac1
Yue et al., J Steroid Biochem Mol Biol 2003 (Breast Neoplasms...) : In aromatase over expressing MCF-7 cells, aromatase activity was reduced by inhibitors of MAPK and PI3K suggesting the involvement of protein phosphorylation in the regulation of aromatase activity
Middleton et al., J Exp Med 2006 (Myeloproliferative Disorders) : These data identify 12/15-LO as an important suppressor of MPD via its role as a critical upstream effector in the regulation of PI3-K dependent ICSBP phosphorylation
Gingery et al., J Cell Biochem 2003 (MAP Kinase Signaling System) : PI3K inhibition also blocked MEK1/2, ERK1/2 , and AKT phosphorylation and NFkappaB activation in purified osteoclasts ... PI3K inhibition also blocked MEK1/2, ERK1/2, and AKT phosphorylation and NFkappaB activation in purified osteoclasts ... PI3K inhibition also blocked MEK1/2 , ERK1/2, and AKT phosphorylation and NFkappaB activation in purified osteoclasts ... PI3K inhibition also blocked MEK1/2, ERK1/2 , and AKT phosphorylation and NFkappaB activation in purified osteoclasts ... PI3K inhibition also blocked MEK1/2, ERK1/2, and AKT phosphorylation and NFkappaB activation in purified osteoclasts
Tfelt-Hansen et al., Endocrinology 2004 (Hypercalcemia...) : Taken together, our data show that activation of PI3K and p38 MAPK but not of MEK1/ERK by the CaR promotes proliferation of H-500 cells as well as affords protection against apoptosis
Hatano et al., Am J Physiol Gastrointest Liver Physiol 2001 : The PI3K inhibitor LY-294002 blocks TNF-alpha- and Fas mediated Akt phosphorylation ... The PI3K inhibitor LY-294002 blocks TNF-alpha- and Fas mediated Akt phosphorylation
Banerjee et al., Breast Cancer Res Treat 2012 (Breast Neoplasms...) : Pg extract also induced SHIP-1 expression and this was accompanied by downregulation of miRNA-155 and inhibition of PI3K dependent phosphorylation of AKT
Silfani et al., Arch Biochem Biophys 2002 : However, it is not known whether PI3K is involved in the Ang II-induced activation of cPLA(2) or if this mechanism is associated with the Ang II-mediated growth of VSMC ... Therefore, we used cultured rat VSMC to examine the role of PI3K in the Ang II-dependent phosphorylation of cPLA(2) , release of AA, and growth induced by Ang II
Chern et al., Planta Med 2011 (Cerebral Infarction...) : Andrographolide inhibits PI3K/AKT dependent NOX2 and iNOS expression protecting mice against hypoxia/ischemia induced oxidative brain injury ... Andrographolide inhibits PI3K/AKT dependent NOX2 and iNOS expression protecting mice against hypoxia/ischemia induced oxidative brain injury ... Our results indicate that andrographolide reduces NOX2 and iNOS expression possibly by impairing PI3K/AKT dependent NF- ?B and HIF-1 a activation
Yang et al., Proc Natl Acad Sci U S A 2009 : APC also induced phosphorylation of Ser-9 in glycogen synthase kinase 3beta ( GSK3beta ), which was blocked by the PI3K inhibitor LY294002 ... Thus, we propose that ligation of ApoER2 by APC signals via Dab1 phosphorylation and subsequent activation of PI3K and Akt and inactivation of GSK3beta, thereby contributing to APC 's beneficial effects on cells
Heydrick et al., J Clin Invest 1993 (Diabetes Mellitus, Type 2...) : Insulin did not affect total PI3K activity
Henao-Mejia et al., Immunity 2013 : Mechanistically, miR-181 modulated expression of the phosphatase PTEN to control PI3K signaling, which was a primary stimulus for anabolic metabolism in immune cells
Liu et al., Biochim Biophys Acta 2007 (MAP Kinase Signaling System) : We propose that the inhibition of PI(3)K by wortmannin in mouse macrophages enhances the PI-PLC downstream signals, and subsequently increases the LPS induction of iNOS expression independently of Akt pathway ... We propose that the inhibition of PI(3)K by wortmannin in mouse macrophages enhances the PI-PLC downstream signals, and subsequently increases the LPS induction of iNOS expression independently of Akt pathway
Wang et al., Am J Physiol Cell Physiol 2010 : Simultaneous inhibition of PKA and PI3K reduces IL-6 expression in stimulated chondrocytes well below the basal levels of untreated cells
Oh et al., Int Immunopharmacol 2011 : CKD712, ( S ) -1- ( a-naphthylmethyl ) -6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, inhibits the lipopolysaccharide stimulated secretion of HMGB1 by inhibiting PI3K and classical protein kinase C
Gallagher et al., Oncogene 2012 (Cell Transformation, Neoplastic...) : We also investigated the effect of targeted PI3K/mTOR inhibition on PI3K/Akt/mTOR and Erk1/2 signaling, and the potential effects on glycemia ... We also investigated the effect of targeted PI3K/mTOR inhibition on PI3K/Akt/mTOR and Erk1/2 signaling, and the potential effects on glycemia ... We also investigated the effect of targeted PI3K/mTOR inhibition on PI3K/Akt/mTOR and Erk1/2 signaling, and the potential effects on glycemia ... We also investigated the effect of targeted PI3K/mTOR inhibition on PI3K/Akt/mTOR and Erk1/2 signaling, and the potential effects on glycemia
García et al., Leuk Res 2009 (Lymphoma) : PI3K/Akt inhibition modulates multidrug resistance and activates NF-kappaB in murine lymphoma cell lines
Reagan-Shaw et al., Cancer Res 2006 (Breast Neoplasms...) : Further, our data showed that PI3K knockdown resulted in a significant activation of FoxO ; interestingly, a simultaneous knockdown of FoxO1a rescued the cells from apoptosis
Liu et al., J Cell Biochem 2009 : A specific PI3K inhibitor, wortmannin, blocked Akt phosphorylation and abrogated the beneficial effect of DMOG
Li et al., Zhongguo Dang Dai Er Ke Za Zhi 2011 (Neuroblastoma) : The synthesis and secretion of VEGF can be inhibited by blocking TrkB-BDNF signal pathway with K252a or blocking the TrkB-BDNF downstream signal pathway PI3K/Akt with LY294002
Li et al., J Biol Chem 2008 : Inhibition of PI3K or mTOR reduced the level of Rap1B , which acts downstream of Rheb and mTOR
Kang et al., Mol Pharmacol 2002 : Phosphatidylinositol 3-kinase regulates nuclear translocation of NF-E2 related factor 2 through actin rearrangement in response to oxidative stress
Liu et al., J Exp Med 2007 (Toxoplasmosis) : We further show that DGKzeta negatively controls the phosphatidylinositol 3-kinase (PI3K)-Akt pathway and that inhibition of PI3K activity or treatment with PA can restore lipopolysaccharide induced IL-12 production by DGKzeta-deficient Mphi
Hosoi et al., Eur J Pharmacol 2007 : Inhibitory effect of 4- ( 2-aminoethyl ) -benzenesulfonyl fluoride, a serine protease inhibitor, on PI3K inhibitor induced CHOP expression
Furuya et al., J Biol Chem 2013 : The inhibition of p85a expression by siRNA or the inhibition of PI3K by LY294002 prevents the expression of Pdx1, Ngn3, and MafA and the reprogramming to insulin producing cells
Morrison et al., Am J Physiol Endocrinol Metab 2005 : Because NPY/AgRP neurons project to the hypothalamic paraventricular nucleus ( PVN ), we next investigated whether leptin activation of PVN neurons is similarly PI3K dependent ... We conclude that leptin requires intact PI3K signaling both to inhibit hypothalamic Npy and Agrp gene expression and activate neurons within the PVN ... We conclude that leptin requires intact PI3K signaling both to inhibit hypothalamic Npy and Agrp gene expression and activate neurons within the PVN ... We conclude that leptin requires intact PI3K signaling both to inhibit hypothalamic Npy and Agrp gene expression and activate neurons within the PVN
Li et al., Cancer Lett 2009 (Adenocarcinoma...) : Arsenic trioxide induces apoptosis and G2/M phase arrest by inducing Cbl to inhibit PI3K/Akt signaling and thereby regulate p53 activation
Ghosh et al., J Biol Chem 2012 (MAP Kinase Signaling System...) : Therefore, based on these observations, it is hypothesized that the activation of PI3K/AKT and p38 MAPK by VEGF results in ETS-1 gene expression, which activates MMP-9 and MMP-13, leading to the invasion and scattering of SKOV-3 cells ... Therefore, based on these observations, it is hypothesized that the activation of PI3K/AKT and p38 MAPK by VEGF results in ETS-1 gene expression, which activates MMP-9 and MMP-13, leading to the invasion and scattering of SKOV-3 cells
Pan et al., J Agric Food Chem 2008 : We found that pterostilbene also inhibited LPS induced activation of PI3K/Akt , extracellular signal regulated kinase 1/2 and p38 MAPK ... Taken together, these results show that pterostilbene down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFkappaB by interfering with the activation of PI3K/Akt/IKK and MAPK ... Taken together, these results show that pterostilbene down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFkappaB by interfering with the activation of PI3K/Akt/IKK and MAPK
Li et al., J Ethnopharmacol 2012 (Arthritis, Experimental) : To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen induced arthritis ( CIA ) ... To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen induced arthritis ( CIA ) ... PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats ... PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats
Gesbert et al., J Biol Chem 2000 : The PI3K inhibitor LY-294002 blocks the ability of BCR/ABL to induce p27 ( Kip1 ) down-regulation and inhibits BCR/ABL induced entry into S phase
Bertagnolo et al., Exp Cell Res 2005 (Leukemia, Promyelocytic, Acute) : We have previously demonstrated that the amount and the tyrosine phosphorylation of Vav are up-regulated in both whole cells and nuclei of tumoral promyelocytes induced to granulocytic maturation by ATRA and that tyrosine phosphorylated Vav does not display any ATRA induced GEF activity but contributes to the regulation of PI 3-K activity
Mortaz et al., Respir Res 2009 : Moreover, CSE suppressed PI3K/Akt signalling in pDC
Mawrin et al., Clin Cancer Res 2005 (Meningeal Neoplasms...) : Atypical and malignant meningiomas showed higher levels of phospho-Akt compared with benign tumors, and their proliferation could be inhibited by PI3K blocking using wortmannin
Debiais et al., Exp Cell Res 2004 : FGF-2 increased PI3K activity but did not induce phosphorylation of Akt or the downstream effector p70 S6 kinase ... FGF-2 increased PI3K activity but did not induce phosphorylation of Akt or the downstream effector p70 S6 kinase ... The results indicate that the immediate protective effect of FGF-2 on human osteoblastic cell apoptosis involves PI3K and inhibition of downstream caspases, independently of GSK-3 and beta-catenin-Lef/Tcf mediated transcription
Halevy et al., Exp Cell Res 2004 (MAP Kinase Signaling System) : UO126, a specific MAPK pathway inhibitor, had no effect on PI3K activity or Akt phosphorylation, implying that at least in muscle cells, the MAPK/ERK pathway is not required for HGF induced PI3K activation ... Moreover, the finding that PI3K activity is required for HGF induced MAPK activation suggests its additional role in proliferation, rather than exclusively in the differentiation of adult myoblasts
Lee et al., J Cell Biochem 2012 (Chondrosarcoma) : The phosphatidylinositol 3-kinase (PI3K), Akt , and NF-?B pathways were activated by MIF treatment, and the MIF induced expression of integrin and migration activity were inhibited by the specific inhibitors and mutant forms of PI3K , Akt, and NF-?B cascades ... The phosphatidylinositol 3-kinase (PI3K) , Akt, and NF-?B pathways were activated by MIF treatment, and the MIF induced expression of integrin and migration activity were inhibited by the specific inhibitors and mutant forms of PI3K , Akt, and NF-?B cascades
Hwang et al., Int J Hematol 2006 : IFN-gamma did not enhance SDF-1 induced activation of PI3K/Akt or up-regulate the expression of CXCR4 or its function in bone marrow CD34+ cells ... IFN-gamma did not enhance SDF-1 induced activation of PI3K/Akt or up-regulate the expression of CXCR4 or its function in bone marrow CD34+ cells
Paradis et al., Genes Dev 1998 : This demonstrates that Akt/PKB activity is not necessarily dependent on AGE-1 PI3K activity ... This demonstrates that Akt/PKB activity is not necessarily dependent on AGE-1 PI3K activity
Belletti et al., J Biol Chem 2001 : The role of PI3K activity in the up-regulation of Id2 gene expression by the IGF-IR was confirmed by different methods and in different cell types
Han et al., Molecular cancer 2010 (Disease Models, Animal...) : Co-treatment with NF-kappaB , STAT3 or/and PI3K inhibitors led to additive inhibition of iMyc E mu-1 cell proliferation, suggesting that these signaling pathways converge
Yaspelkis et al., Life Sci 2004 : Leptin appeared to mediate these effects by normalizing insulin stimulated PI3-K activation and GLUT4 protein concentration in the RG
Girart et al., J Immunol 2007 (Melanoma) : Excitingly, IFN-gamma secretion was significantly increased when NK cells were stimulated with poly ( I:C ) or loxoribine and IL-12, and NKG2D engagement was induced by coculture with MICA+ tumor cells in a PI3K dependent manner
Gingerich et al., Neuropharmacology 2008 : Finally, we demonstrate that Src kinase acts upstream of the PI3K/Akt pathway based on our finding that the selective Src inhibitor, PP2 ( 10microM ), blocked the increases in nNOS phosphorylation levels, NO production, and PI3K/Akt activity induced by ERbeta activation ... Finally, we demonstrate that Src kinase acts upstream of the PI3K/Akt pathway based on our finding that the selective Src inhibitor, PP2 ( 10microM ), blocked the increases in nNOS phosphorylation levels, NO production, and PI3K/Akt activity induced by ERbeta activation
Ueno et al., Dev Growth Differ 2011 : In contrast, the activation of PI3K-TOR by Rheb-S16H expression delayed the initiation of cell cycle elongation
Yoon et al., Mol Cancer Ther 2006 (Breast Neoplasms...) : H-Ras did not increase MMP-9 in the presence of a PI3K inhibitor, LY294002, and a NF-kappaB inhibitor, SN50 ... H-Ras did not increase MMP-9 in the presence of a PI3K inhibitor, LY294002, and a NF-kappaB inhibitor, SN50
Zhang et al., J Mol Endocrinol 2005 (Breast Neoplasms...) : Moreover, these same IGF-I induced responses were also inhibited by the antiestrogen ICI 182780 and this was in contrast to a previous report suggesting that ICI 182780 did not affect IGF-I dependent activation of PI3-K or induction of cyclin D1 expression
Zhang et al., J Biol Chem 2003 : Phosphorylation of VEGFR2 at Tyr-801 and Tyr-1175, the critical sites for VEGF induced PI3K-Akt signaling, was not involved in TNF mediated Akt activation ... Taken together, our data demonstrated that TNF induces transactivation between Etk and VEGFR2, and Etk directly activates PI3K-Akt angiogenic signaling independent of VEGF induced VEGFR2-PI3K-Akt signaling pathway ... Taken together, our data demonstrated that TNF induces transactivation between Etk and VEGFR2, and Etk directly activates PI3K-Akt angiogenic signaling independent of VEGF induced VEGFR2-PI3K-Akt signaling pathway
Troutman et al., Cell cycle (Georgetown, Tex.) 2012 (Inflammation) : Here, we summarize the current understanding of signaling pathways activated by TLRs and provide our perspective on TLR mediated activation of PI3K and its impact on regulating cellular processes
Zhuang et al., Ann Clin Lab Sci 2011 (Subarachnoid Hemorrhage) : Rats were randomly divided into 6 groups : control group, SAH group, SAH+ saline group, SAH+ vehicle group, SAH+ Insulin-like Growth Factor 1 (IGF-1) group, and SAH+Ly294002 ( PI3K pathway inhibitor ) group
Wee et al., Cancer Res 2009 (Colonic Neoplasms) : The oncogenic potential of these effector pathways is illustrated by the frequent occurrence of activating mutations in BRAF and PIK3CA as well as loss-of-function mutations in the tumor suppressor PTEN , a negative regulator of PI3K
Fan et al., Science signaling 2009 (Brain Neoplasms...) : Inhibition of EGFR signaling correlated with decreased abundance of phosphorylated mTOR ( p-mTOR ) and rpS6 ( p-rpS6 ) in cells wild type for the gene encoding PTEN ( phosphatase and tensin homolog on chromosome 10 ), a negative regulator of PI3K
Lai et al., J Huazhong Univ Sci Technolog Med Sci 2012 : Western blotting was performed to examine the effect of EMP-1 on the PI3K/AKT signaling
Bertagnolo et al., Cell Signal 2004 : The Vav/p85 interaction is essential for the ATRA induced PI 3-K activity and for association of PI 3-K with actin, particularly in the nucleus ... The Vav/p85 interaction is essential for the ATRA induced PI 3-K activity and for association of PI 3-K with actin, particularly in the nucleus
Bian et al., Am J Physiol Heart Circ Physiol 2009 (Disease Models, Animal...) : Inhibition of erbB2, phosphoinositide 3-kinase (PI3K) , Akt, and mTOR blocked the protective effects of NRG1 on cTnI and cTnT in NRVM
Wang et al., J Bone Miner Res 2010 : T ( 3 ) activates IGF-1/IGF1R signaling and IGF-1 dependent PI3K/Akt/GSK-3beta signaling in growth plate chondrocytes undergoing proliferation and differentiation to prehypertrophy ... T ( 3 ) -mediated Wnt-4 expression, beta-catenin activation, cell proliferation, and terminal differentiation of growth plate chondrocytes are partially prevented by the IGF1R inhibitor picropodophyllin as well as by the PI3K/Akt signaling inhibitors LY294002 and Akti1/2 ... T ( 3 ) -mediated Wnt-4 expression, beta-catenin activation, cell proliferation, and terminal differentiation of growth plate chondrocytes are partially prevented by the IGF1R inhibitor picropodophyllin as well as by the PI3K/Akt signaling inhibitors LY294002 and Akti1/2
Fortin et al., Eur J Immunol 2011 : Finally, we show that in LPS- and TNF activated neutrophils, PI3K acts downstream of the kinases p38 MAPK and TAK1
Fernández et al., Biochim Biophys Acta 2008 : We analyzed IGF-I signalling on astrocytes through the canonical phosphatidylinositol 3-kinase (PI3K)/Akt pathway and focused on possible changes in PTEN, a phosphatase that modulates IGF-I signalling by inhibiting Akt activation and, in turn is positively regulated by PI3K ... Subsequent recovery of reduced PTEN required also activation by IGF-I of PI3K to recruit in this case protein kinase A (PKA) which stimulated Egr-1 levels and, consequently PTEN synthesis
Xing et al., Journal of neuroinflammation 2008 (Encephalitis) : Elevations of phosphorylated PPAR-gamma, PI3K, and Akt levels were observed with pioglitazone treatment, and inhibition of PI3K activity enhanced LPS induced NO production
Abid et al., Arterioscler Thromb Vasc Biol 2008 : Incubation of human coronary artery endothelial cells with HGF induced prolonged PI3K/Akt dependent phosphorylation and nuclear exclusion of FKHR/FOXO1
Venkatachalam et al., Am J Physiol Heart Circ Physiol 2008 (Hyperglycemia) : Resveratrol inhibits high glucose induced PI3K/Akt/ERK dependent interleukin-17 expression in primary mouse cardiac fibroblasts ... HG induces phosphoinositide 3- kinase [PI3K ; inhibited by adenoviral ( Ad ) .dominant negative ( dn ) PI3Kp85 ], Akt ( inhibited by Ad.dnAkt1 ), and ERK ( inhibited by PD-98059 ) activation and induces IL-17 expression via PI3K -- > Akt -- > ERK dependent signaling
Patel et al., Diabetes 2003 : None of the inhibitors significantly inhibited protein content over 20 days, but lipid content and lipogenic activity were inhibited by p70 ( S6 ) kinase and p38 MAPK inhibition but not by p42/44 MAPK or PI3K inhibition ... None of the inhibitors significantly inhibited protein content over 20 days, but lipid content and lipogenic activity were inhibited by p70 ( S6 ) kinase and p38 MAPK inhibition but not by p42/44 MAPK or PI3K inhibition ... None of the inhibitors significantly inhibited protein content over 20 days, but lipid content and lipogenic activity were inhibited by p70 ( S6 ) kinase and p38 MAPK inhibition but not by p42/44 MAPK or PI3K inhibition
Lee et al., Cell cycle (Georgetown, Tex.) 2008 (Prostatic Neoplasms) : Inhibition of PI3K with either LY294002 and wortmannin was sufficient to cause upregulation of ERK activity as measured by immunoblotting
Beitz et al., J Biol Chem 1999 : Whereas CD19 does not appear to be involved in this SYK dependent pathway, the SYK substrate CBL is likely involved as the dominant negative SYK markedly attenuates CBL tyrosine phosphorylation and completely blocks the BCR dependent association of CBL with p85 PI3K
Huang et al., J Cell Biochem 2012 : Inhibition of calcineurin further reduced the phosphorylation of ERK and AKT ( at thr 308 ) and inhibited the activation of Ras, but inhibitors of MAPK or PI3K signaling did not affect the circadian rhythm of calcineurin activity ... Inhibition of calcineurin further reduced the phosphorylation of ERK and AKT ( at thr 308 ) and inhibited the activation of Ras, but inhibitors of MAPK or PI3K signaling did not affect the circadian rhythm of calcineurin activity
Sedding et al., Circ Res 2005 : Furthermore, caveolin-1 is essential for the integrin mediated activation of PI3-K/Akt
Li et al., Biomed Environ Sci 2010 : Activation of PI3K/Akt1 pathway and subsequent negative regulation of p21 ( WAF1/CIP1 ) and p27 ( KIP1 ) expression might be involved in CagA associated carcinogenesis ... Activation of PI3K/Akt1 pathway and subsequent negative regulation of p21 ( WAF1/CIP1 ) and p27 ( KIP1 ) expression might be involved in CagA associated carcinogenesis
Klöckener et al., Nat Neurosci 2011 (Body Weight...) : Collectively, our experiments reveal that high-fat diet induced, insulin dependent PI3K activation in VMH neurons contributes to obesity development
Qiao et al., Cancer Lett 2006 (Neuroblastoma) : GRP induced activation of PI3-K , resulting in neuroblastoma cell growth promotion, is potentiated by down-regulation of TGF-beta/Smad signaling ... GRP induced activation of PI3-K , resulting in neuroblastoma cell growth promotion, is potentiated by down-regulation of TGF-beta/Smad signaling
Xu et al., Eur Rev Med Pharmacol Sci 2011 (Diabetes Mellitus, Experimental) : Effect of insulin in combination with selenium on blood glucose and PI3K mediated GLUT4 expression in skeletal muscle of streptozotocin induced diabetic rats
Bradley et al., J Cell Biochem 2008 : M-CSF activates Ras to coordinate activation of PI3K and Raf/MEK/ERK , since Ras inhibition decreased PI3K activation and PI3K inhibition did not block M-CSF mediated Ras activation
Wang et al., J Am Chem Soc 2008 : To explore the specificity of these types of molecules, we have synthesized D-3-deoxydioctanoylphosphatidylinositol ( D-3-deoxy-diC8PI ), D-3,5-dideoxy-diC8PI, and D-3-deoxy-diC8PI-5-phosphate and their enantiomers, characterized their aggregate formation by novel high-resolution field cycling ( 31 ) P NMR, and examined their susceptibility to phospholipase C (PLC) , their effects on the catalytic activities of PI3K and PTEN against diC8PI and diC8PI-3-phosphate substrates, respectively, and their ability to induce the death of U937 human leukemic monocyte lymphoma cells
Li et al., Brain Res Mol Brain Res 2003 (Amyotrophic Lateral Sclerosis...) : VEGF activated both PI3-K and MAPK activities in mouse NSC34 motor neuron-like cells
Garçon et al., Blood 2008 : CD28 provides T-cell costimulation and enhances PI3K activity at the immune synapse independently of its capacity to interact with the p85/p110 heterodimer ... Using a quantitative imaging based assay, we show that the PI3K activity at the T cell-APC contact area is dependent on the p110delta , but not the p110gamma, isoform of PI3K
Palacio et al., Eur J Endocrinol 2001 : In murine osteoclast-like cells grown from bone marrow cells co-cultured with osteoblasts, the activation of the PI 3-K by CSF-1 was determined both in vivo and in vitro ... In osteoclast-like cells, CSF-1 stimulated PI 3-K activity ... In osteoclast-like cells, CSF-1 stimulated PI 3-K activity
Pan et al., BJU Int 2009 (Urinary Bladder Neoplasms) : Fibronectin mediated PI3-K/Akt activation protects T24 cells from MMC induced cell death through inhibition of both caspase-9 and AIF mediated apoptosis and GSK-3beta/cyclin D1 involved G0/G1-phase arrest
Zawalich et al., J Endocrinol 2002 (Obesity) : They also suggest that, as insulin resistance develops in peripheral tissues, a potential result of impaired PI3K activation, the same biochemical anomaly in beta-cells promotes a linked increase in insulin secretion to maintain glucose homeostasis
Fransson et al., Journal of molecular signaling 2013 : PI3K activity subsequently activates Akt/PKB , and as mutations of PI3K are rare in neuroblastoma and high levels of PI3K subunit p110delta is associated with favorable disease with low p-Akt/PKB, the levels of other PI3K subunits could be important for Akt activation ... PI3K activity subsequently activates Akt/PKB , and as mutations of PI3K are rare in neuroblastoma and high levels of PI3K subunit p110delta is associated with favorable disease with low p-Akt/PKB, the levels of other PI3K subunits could be important for Akt activation
Wang et al., Biochem J 2007 : We have shown previously that PAR-2 simultaneously promotes Galphaq/Ca2+ dependent activation and beta-arrestin-1 dependent inhibition of class IA PI3K ( phosphoinositide 3-kinase ), and we sought to characterize further the role of beta-arrestins in the regulation of PI3K activity ... We have shown previously that PAR-2 simultaneously promotes Galphaq/Ca2+ dependent activation and beta-arrestin-1 dependent inhibition of class IA PI3K ( phosphoinositide 3-kinase ), and we sought to characterize further the role of beta-arrestins in the regulation of PI3K activity ... In the present study we have demonstrated that : ( i ) PAR-2 increases p110alpha- and p110beta associated lipid kinase activities, and both p110alpha and p110beta are inhibited by over-expression of either beta-arrestin-1 or -2 ; ( ii ) both beta-arrestin-1 and -2 directly inhibit the p110alpha catalytic subunit in vitro, whereas only beta-arrestin-2 directly inhibited p110beta ; ( iii ) examination of upstream pathways revealed that PAR-2 induced PI3K activity required the small GTPase Cdc ( cell-division cycle)42, but not tyrosine phosphorylation of p85 ; and ( iv ) beta-arrestins inhibit PAR-2 induced Cdc42 activation ... In the present study we have demonstrated that : ( i ) PAR-2 increases p110alpha- and p110beta associated lipid kinase activities, and both p110alpha and p110beta are inhibited by over-expression of either beta-arrestin-1 or -2 ; ( ii ) both beta-arrestin-1 and -2 directly inhibit the p110alpha catalytic subunit in vitro, whereas only beta-arrestin-2 directly inhibited p110beta ; ( iii ) examination of upstream pathways revealed that PAR-2 induced PI3K activity required the small GTPase Cdc ( cell-division cycle)42, but not tyrosine phosphorylation of p85 ; and ( iv ) beta-arrestins inhibit PAR-2 induced Cdc42 activation ... In the present study we have demonstrated that : ( i ) PAR-2 increases p110alpha- and p110beta associated lipid kinase activities, and both p110alpha and p110beta are inhibited by over-expression of either beta-arrestin-1 or -2 ; ( ii ) both beta-arrestin-1 and -2 directly inhibit the p110alpha catalytic subunit in vitro, whereas only beta-arrestin-2 directly inhibited p110beta ; ( iii ) examination of upstream pathways revealed that PAR-2 induced PI3K activity required the small GTPase Cdc ( cell-division cycle)42, but not tyrosine phosphorylation of p85 ; and ( iv ) beta-arrestins inhibit PAR-2 induced Cdc42 activation
Nguyen et al., Ann N Y Acad Sci 2005 : Relaxin also stimulates protein kinase C zeta (PKCzeta) translocation to the plasma membrane in a PI3K dependent manner in THP-1 and MCF-7 cells
Brennan et al., Immunity 1997 : Inhibition of PI3K inhibits phosphorylation of Rb, induction of cyclin D3 , and degradation of p27kip1 ... Inhibition of PI3K inhibits phosphorylation of Rb, induction of cyclin D3, and degradation of p27kip1 ... Inhibition of PI3K inhibits phosphorylation of Rb, induction of cyclin D3, and degradation of p27kip1
Qiao et al., Hepatology 2002 : Activation of MAPK was partially blocked by inhibitors of PI3K
Gulhati et al., Carcinogenesis 2012 (Colorectal Neoplasms...) : In this study, we show that inhibition of mTORC1 with rapamycin leads to feedback activation of PI3K/Akt and Ras-MAPK signaling, resulting in cell survival and possible contribution to rapamycin resistance
Cichy et al., J Biol Chem 1998 : Constitutively active PI3K and PKB/Akt are each sufficient to mediate effects of insulin on the IGFBP-1 promoter in a nonadditive fashion
Parhar et al., Mol Cell Biochem 2007 : p65 ( Rel A ) Ser536 phosphorylation was not affected by the PI3K inhibitors but was dose-dependently attenuated by TPCK ... p65 ( Rel A ) Ser536 phosphorylation was not affected by the PI3K inhibitors but was dose-dependently attenuated by TPCK
Chen et al., EMBO Rep 2001 : However, in this report we demonstrate that inhibition of epidermal growth factor (EGF) stimulated PI3K activity by expression of the kinase-deficient PI3K p110 subunit ( p110delta kin ) does not block the lysosomal targeting and degradation of the EGF receptor (EGFR)
Fiory et al., J Biol Chem 2004 : PI3K inhibition also reduced insulin degradation and intact insulin release by 50 and 75 %, respectively
Okamoto et al., Biochem J 1993 : These results indicate that the specific activity of PI3K is increased by insulin stimulation without detectable tyrosine phosphorylation of PI3K itself in Fao cells ... The majority of the insulin activated portion of PI3K is associated with pTyr containing proteins including IRS-1, which suggests that this is important for activation of PI3K by insulin
Kuo et al., Oncogene 2001 : The involvement of PI 3-K/Akt dependent up-regulation of Mcl-1 in the prevention of apoptosis of Hep3B cells by interleukin-6 ... Our previous works have demonstrated that both PI 3-K/Akt and STAT3 pathways were concomitantly activated and cooperatively mediated the anti-apoptotic effect of IL-6 ... However, the IL-6 induced Mcl-1 up-regulation was effectively attenuated in the presence of PI 3-K inhibitors, LY294002 and wortmannin
Jensen et al., J Pathol 2010 (Thyroid Neoplasms) : Inhibition of PI3K/AKT or MAPK/ERK signalling did not affect the level of nectin-1 expression but decreased thyroid cancer cell susceptibility to HSV
Yin et al., J Mol Med (Berl) 2011 (Disease Models, Animal...) : Our results demonstrated that administration of Rg1 increased VEGF expression levels, activated PI3K/Akt , and inhibited p38 MAPK in vitro and in vivo ... PI3K inhibitor LY294002 significantly attenuated Rg1 enhanced VEGF expression and capillary density ... Rg1 induced activation of PI3K/Akt also contributed to the downregulation of p38 MAPK ... Rg1 induced activation of PI3K/Akt also contributed to the downregulation of p38 MAPK
York et al., Mol Cell Biol 2000 : We show here that Rap1 activation requires both TrkA internalization and PI3-K , whereas Ras activation requires neither TrkA internalization nor PI3-K ... Both inhibitors of PI3-K and inhibitors of endocytosis prevent GTP loading of Rap1 and block sustained ERK activation by NGF ... PI3-K and endocytosis may also regulate ERK signaling at a second site downstream of Ras, since both rapid ERK activation and the Ras dependent activation of the MAP kinase kinase kinase B-Raf are blocked by inhibition of either PI3-K or endocytosis ... PI3-K and endocytosis may also regulate ERK signaling at a second site downstream of Ras, since both rapid ERK activation and the Ras dependent activation of the MAP kinase kinase kinase B-Raf are blocked by inhibition of either PI3-K or endocytosis
Walter et al., Biochem Biophys Res Commun 2008 (Carcinoma, Hepatocellular) : This suppressive effect of insulin on SeP expression was attenuated by inhibitors of PI3K
Di Guglielmo et al., Mol Cell Biochem 1998 : Furthermore, studies with isolated rat adipocytes reveal the EN to be the principle site of insulin stimulated IRS-1 tyrosine phosphorylation and associated PI3K activation
Hermanto et al., Mol Cell Biol 2002 : While IGF-I induced activation of IRS-1, Shc, PI3K , and MAPK pathways was unaffected, IGF-I-inducible beta1 integrin associated kinase activity and association of Crk with p130 ( CAS ) were significantly inhibited by RACK1 overexpression
Takahashi et al., Am J Physiol 1999 : These results indicate that ANG II stimulates Akt/PKB activity via AT1 receptors in VSMC and that the activities of tyrosine kinase and PI3K are required for this activation ... These results indicate that ANG II stimulates Akt/PKB activity via AT1 receptors in VSMC and that the activities of tyrosine kinase and PI3K are required for this activation
Kiaer et al., Endocrine 2009 : In conclusion, it is suggested that albumin may be a survival factor for pancreatic beta cells through scavenging of reactive oxygen species and by PI3K dependent activation of Akt
Beaulieu et al., PloS one 2011 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive) : The effect of a-linolenic acids seemed to be due to PI3K pathway and Bcl-2 inhibition
Bhattacharya et al., J Neurooncol 2013 (Brain Neoplasms...) : T11TS therapy in rodent glioma model significantly downregulated expression of VEGF along with its receptor VEGFR-2 and inhibited the expression of pro-survival PI3K/Akt/eNOS proteins in glioma associated brain endothelial cells
Tung et al., J Cell Physiol 2010 (MAP Kinase Signaling System) : Inhibition of PI3K by Wortmannin attenuated EV71 induced Akt and p42/p44 MAPK phosphorylation, but had no effect on PDGFR phosphorylation, suggesting that PDGFR is an upstream and p42/p44 MAPK is a downstream component of PI3K/Akt in these responses ... Inhibition of PI3K by Wortmannin attenuated EV71 induced Akt and p42/p44 MAPK phosphorylation, but had no effect on PDGFR phosphorylation, suggesting that PDGFR is an upstream and p42/p44 MAPK is a downstream component of PI3K/Akt in these responses
Díaz-Montero et al., Eur J Cancer 2006 (Osteosarcoma) : PI3-K/Akt mediated anoikis resistance of human osteosarcoma cells requires Src activation ... The activity of Akt was found to be upregulated in anoikis resistant SAOSar cells and the pharmacological inhibition of PI3-K activity restored sensitivity to anoikis resistant cells, reconfirming the critical role of PI3-K/Akt pathway in cell survival
Rani et al., J Biol Chem 2002 (Fibrosarcoma) : Studies with PTEN mutants suggested that the lipid kinase activity of PI3K was essential for IFN-beta induced transcription of beta-R1
Matsuda et al., Blood 2009 : Critical role of class IA PI3K for c-Rel expression in B lymphocytes ... Nevertheless, both PI3K and Btk are required for the activation of NF-kappaB , a critical transcription factor family for B-cell development and function ... The loss of PI3K activity results in marked reduction of c-Rel and to a lesser extent RelA expression ... These results show that the PI3K mediated control of c-Rel expression is essential for B-cell functions
Hart et al., Proc Natl Acad Sci U S A 2011 (Cell Transformation, Neoplastic) : GDC-0941, a specific inhibitor of PI3K reduces the level of Stat3 phosphorylation ... The effect of PI3K on Stat3 appears to be mediated by a member of the Tec kinase family ... In some human tumor cell lines, the enhanced phosphorylation of Stat3 is inhibited by both PI3K and by Tec kinase inhibitors, suggesting that the link between PI3K and Stat3 is significant in human cancer
Caparrós et al., Blood 2006 (Calcium Signaling...) : DC-SIGN ligation on dendritic cells results in ERK and PI3K activation and modulates cytokine production
Lee et al., Mol Biol Cell 2012 : We demonstrate that endoglin interacts with the PI3K subunits p110a and p85 via GIPC to recruit and activate PI3K and Akt at the cell membrane
Pore et al., Cancer Res 2003 (Glioblastoma) : In the current study we investigated the effect of PTEN , a negative regulator of PI3K signaling commonly mutated in glioblastoma cells, on VEGF expression
Tang et al., Cancer Lett 2012 (Bone Neoplasms...) : Activations of phosphatidylinositol 3-kinase (PI3K) , Akt, and AP-1 pathways after HMGB-1 treatment were demonstrated, and HMGB-1 induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K , Akt, and AP-1 cascades
Li et al., Am J Physiol 1999 : IGF-I activated a wortmannin-sensitive PI3K and its purported effector, the atypical protein kinase C (PKC)-zeta
Imai et al., Life Sci 2003 (Calcium Signaling) : SGK1 is one of the protein-serine/threonine kinases that is activated by insulin in a PI3K dependent manner
Miyakawa et al., J Biol Chem 2001 : In primary murine MKs, TPO also induced phosphorylation of SHP2, its association with p85 and enhanced PI3K activity, but in contrast to the results in cell lines, neither Gab2 nor IRS2 are phosphorylated in MKs
Strassheim et al., J Immunol 2005 : SHIP ( -/- ) neutrophils demonstrated significantly increased activation of the PI3K dependent kinase Akt after exposure to PGN
Williams et al., J Neurosci 2011 : The acute effects of leptin require PI3K signaling in the hypothalamic ventral premammillary nucleus
Zacharioudaki et al., J Immunol 2009 : In addition, activation of PI3K and Akt1 also appeared necessary for the induction of IRAK-M by gAd, because treatment of Akt1 ( -/- ) macrophages or pretreatment of macrophages with the PI3K inhibitor wortmannin abolished gAd induced IRAK-M expression
Chiang et al., Oncol Res 2005 (Carcinoma, Hepatocellular) : FAS expression in HepG2 cells was strongly inhibited by PI3K inhibitor LY294002 and JNK inhibitor II and slightly inhibited by p38 inhibitor SB203580 and MEK inhibitor PD98059, separately
Baatar et al., Brain Behav Immun 2009 : Tregs utilize beta-galactoside binding protein to transiently inhibit PI3K/p21ras activity of human CD8+ T cells to block their TCR mediated ERK activity and proliferation
Liu et al., J Surg Res 2004 : In this study, we investigate the role of PLC and PI3-K in platelet derived growth factor ( PDGF ) and extracellular matrix protein ( ECM ) induced SMC proliferation and migration
Li et al., Behav Brain Res 2012 : In cultured PC-12 cells, BDNF activated PI3K/Akt signaling through the TrkB receptor, whereas stimulation of ILK/Akt occurred through TrkA/p75 ( NTR ) heteroreceptor
Elia et al., Biochim Biophys Acta 2007 : Taken together, we suggest that Shh acts in an autocrinic manner in adult myoblasts, and provide first evidence of a role for PI3K/Akt in Shh signaling during myoblast differentiation
Aparicio et al., Mol Reprod Dev 2007 : Inhibition of PI3-K did affect neither the capacitation status nor AR nor protein p32 tyrosine phosphorylation of boar spermatozoa incubated in TBM or TCM
Lee et al., FEBS Lett 2007 : The enhanced MMP-2 activity was significantly attenuated not only by a PI3K inhibitor but also by an Akt inhibitor
Ki et al., Xenobiotica 2008 : 1. alpha-Lipoic acid ( alpha-LA ) activates antioxidant pathways and exerts insulin-like actions via phosphatidylinositol 3-kinase (PI3K), and previous studies from the authors ' laboratory support the essential role of PI3K dependent CCAAT/enhancer binding protein (C/EBP) activation in antioxidant defence responses ... 5. The results demonstrate that alpha-LA induces phase II enzymes via PI3K dependent C/EBPalpha and C/EBPbeta activation, and enhances the ability of insulin to promote target gene induction ... 5. The results demonstrate that alpha-LA induces phase II enzymes via PI3K dependent C/EBPalpha and C/EBPbeta activation, and enhances the ability of insulin to promote target gene induction
Yuan et al., Oncogene 2013 (Adenocarcinoma...) : Further, we used this model to test the efficacy of GDC-0941 , a PI3K inhibitor , in clinical development, and showed that the tumors respond to PI3K inhibition
Amin et al., Int J Oncol 2003 : Similarly, inhibition of PI3K activation by its specific inhibitor LY294002 suppressed Akt phosphorylation
Bruning , Anticancer Agents Med Chem 2013 : Inhibition of the mTOR signaling pathway by quercetin has directly been described and can further be deduced from its interference with PI3K dependent Akt stimulation, AMP dependent protein kinase activation and hamartin upregulation
González et al., Int J Mol Med 2005 (Diabetes Mellitus, Type 2) : Apart from the much higher basal PI3K activity and impaired response to insulin of p44/42 MAP kinases in the diabetic patients, the changes in enzyme activity caused by either hormone or peptide, although not identical, were essentially comparable
Lu et al., Biochem Biophys Res Commun 2004 (Breast Neoplasms...) : They are consistent with the view that the IGF-IR mediated attenuation of trastuzumab induced growth inhibition we recently described is dependent on IGF-I induced PI3K signaling rather than IGF-I induced MAPK signaling
Jiang et al., Frontiers in bioscience : a journal and virtual library 2002 (Diabetes Mellitus, Type 2) : In the current review, we will discuss the role of these molecules in insulin mediated activation of PI3K , the rational for targeting these molecules for diabetes treatment, and some critical issues in terms of drug development
Le et al., Oncogene 2003 : Inhibitors of mTOR, PI3K , and Ca ( ++ ) impair p70S6K activity, whereas inhibitors of JNK and PKC stimulate p70S6K activity
Tian et al., Acta Pharmacol Sin 2004 (Cardiomegaly) : CT-1 simultaneously activated phosphorylation of STAT3, ERK1/2, and PI3-K in rat cardiomyocytes ... The hypertrophic effect of CT-1 was essentially mediated by STAT3 , independent of PI3-K , and negatively regulated by ERK1/2 via inhibiting the phosphorylation of STAT3
Han et al., Invest Ophthalmol Vis Sci 2011 (Glaucoma, Open-Angle) : PI3K inhibition with LY294002 reduced a-SMA expression
Glassford et al., Eur J Immunol 2005 : The inhibitors of PI3K activity, LY294002 and Wortmannin, also abrogate cyclin D2 induction by BCR cross linking, confirming that the class IA PI3K is necessary for cyclin D2 induction in response to BCR stimulation ... Furthermore, using both p85alpha-null and p110delta-null B cells and inhibitors of PI3K, this study demonstrates for the first time, that BCR cross linking induces cyclin D2 mRNA expression via transcriptional activation of the cyclin D2 promoter and that this transcriptional activation of cyclin D2 requires PI3K activity ... Furthermore, using both p85alpha-null and p110delta-null B cells and inhibitors of PI3K, this study demonstrates for the first time, that BCR cross linking induces cyclin D2 mRNA expression via transcriptional activation of the cyclin D2 promoter and that this transcriptional activation of cyclin D2 requires PI3K activity
Hale et al., Proc Natl Acad Sci U S A 2006 : Additionally, it was found that expression of NS1 alone was sufficient to constitutively activate PI3K , causing the phosphorylation of a downstream mediator of PI3K signal transduction, Akt
Hien et al., Mol Pharmacol 2011 : Moreover, AMPK inhibition significantly reversed the activation of ER-dependent transcription and PI3K activation in response to nectandrin B. Nectandrin B evoked endothelium dependent relaxation in rat aortic rings, and this was blocked by inhibition of AMPK , ER, or PI3K
Weber et al., Mol Biol Cell 2001 : Our data suggest that inhibition of initial PI3-K activation by inactive H-Ras or sustained activation of an inhibitory ERK pathway by active H-Ras prevail to abolish LFA-1 regulation and transendothelial migration induced by SDF-1alpha in leukocytes, establishing a complex and bimodal involvement of H-Ras
Miyake et al., J Clin Invest 2002 (Glucose Intolerance...) : Systemic infusion of an adenovirus encoding a dominant negative mutant of PI 3-K ( ( Delta ) p85 ) resulted in liver-specific expression of this protein and in inhibition of the insulin induced activation of PI 3-K in the liver within 3 days, without affecting insulin signaling in skeletal muscle ... The mice also exhibited marked decreases in the serum concentrations of FFAs and triglyceride and suppression of insulin induced PI 3-K activation in adipose tissue, probably due to the associated hyperinsulinemia
Sugimori et al., J Bone Miner Metab 2005 : BMP-2 prevents apoptosis of the N1511 chondrocytic cell line through PI3K/Akt mediated NF-kappaB activation ... Akt was rapidly phosphorylated in response to BMP-2 treatment ; however, the inhibition of PI3K by Wortmannin markedly reduced the phosphorylation of Akt by BMP-2 ... Thus, BMP-2 induced NF-kappaB activation is mediated by PI3K/Akt signaling
Shanmugasundaram et al., Oncogene 2013 (Carcinoma, Renal Cell...) : Here we provide additional genetic evidence that PI3K signaling activates mTORC2 kinase activity
Gigoux et al., Proc Natl Acad Sci U S A 2009 : Although it is known that CD28 mediated PI3K activation is dispensable for GC reaction, the role of ICOS-driven PI3K signaling has not been defined
Velling et al., EMBO Rep 2004 : Here we show that PI3K dependent phosphorylation of Akt in response to ligation of beta1-integrins occurs efficiently in the absence of FAK tyrosine phosphorylation
Niewiadomska et al., Behav Brain Res 2011 (Alzheimer Disease) : NGF released from target cells activates TrkA on axon terminals and triggers activation of PI3K/Akt , MEK/ERK, and PLC? ( phospholipase C ) signaling pathways
Chadborn et al., J Cell Sci 2006 : Sema3A suppresses PI3K signalling concomitant with the activation of GSK-3, which depends on the phosphatase activity of PTEN
Stetler et al., J Neurochem 2012 (Anoxia) : AMPK inhibition suppressed NRF1 and TFAM expression by LPS, whereas PI3K/Akt signaling was necessary for the nuclear translocation of NRF1 and subsequent induction of TFAM ... AMPK inhibition suppressed NRF1 and TFAM expression by LPS, whereas PI3K/Akt signaling was necessary for the nuclear translocation of NRF1 and subsequent induction of TFAM
Amin et al., Blood 2006 : Antisense oligodeoxynucleotides ( ODNs ) and inhibitors of Src, PI3K , p38, and NFkappaB significantly reduced rhMIF induced MN VCAM-1 and ICAM-1 expression ( P < .05 ) ... Antisense oligodeoxynucleotides ( ODNs ) and inhibitors of Src, PI3K , p38, and NFkappaB significantly reduced rhMIF induced MN VCAM-1 and ICAM-1 expression ( P < .05 )
Lavie et al., J Neurochem 1996 (Neuroblastoma) : Preincubation with 1 mM carbachol inhibited the insulin stimulated PI3K activity in a time dependent manner, with half-maximal and maximal inhibition times of 4 and 15 min, respectively ... Addition of the cell-permeable homologue didecanoyl-CDP-DAG to intact cells inhibited insulin stimulated PI3K activity up to 75 %, with an IC50 of 0.5 microM, a result that further supports a proposed lipid mediated inhibition of PI3K
Bhargavi et al., Biochem Mol Biol Int 1998 : Recently Singh et al., ( Biochemistry, 35, 16544-16549, 1996 ) have shown that profilin activates PI3-K activity in a concentration dependent manner
Chen et al., Mol Biol Cell 2005 : MG132 induced DNA binding activity of C/EBPdelta, but not C/EBPbeta was regulated by p38, PI3K , Src, and protein kinase C. Small interfering RNA of C/EBPdelta suppressed COX-2 expression, further strengthening the role of C/EBPdelta in COX-2 gene transcription ... MG132 induced DNA binding activity of C/EBPdelta, but not C/EBPbeta was regulated by p38, PI3K , Src, and protein kinase C. Small interfering RNA of C/EBPdelta suppressed COX-2 expression, further strengthening the role of C/EBPdelta in COX-2 gene transcription
Yamada et al., J Biol Chem 1997 : We examined how BDNF activates PI3-K in cultured cerebral cortical neurons ... IRS-1 and -2 were tyrosine phosphorylated and bound to PI3-K in response to BDNF ... On the other hand, we observed no association of PI3-K with TrkB in response to BDNF ... These results indicate that the activation of TrkB by BDNF induces the activation of PI3-K via IRS-1 and -2 rather than by a direct interaction of TrkB with PI3-K in cultured cortical neurons ... These results indicate that the activation of TrkB by BDNF induces the activation of PI3-K via IRS-1 and -2 rather than by a direct interaction of TrkB with PI3-K in cultured cortical neurons
Lin et al., Int J Cancer 2013 (Brain Neoplasms...) : Palomid 529 lacked affinity for these transporters in vitro, in contrast to GDC-0941 , a small molecule PI3K inhibitor , which we used as control substance for in vitro transport
Balzarolo et al., Eur J Immunol 2012 : Furthermore, PI3K signaling is required for NAB2 mediated TRAIL expression
Watson et al., Arch Dermatol Res 2009 : PI3K-specific inhibitor LY294002 reduced EGF- and HGF stimulated chemokinesis and chemotaxis on collagen I and fibronectin
Morisco et al., Circ Res 2005 (Insulin Resistance) : Short-term stimulation induces an additive effect on insulin induced glucose uptake, and this effect is mediated by phosphorylation of Akt in threonine 308 through PKA/Ca2+ dependent and PI3K independent pathway, whereas insulin evoked threonine phosphorylation of Akt is exclusively PI3K dependent
Myou et al., J Immunol 2003 (MAP Kinase Signaling System) : TAT-Deltap85 and LY294002, a PI3K inhibitor , blocked the phosphorylation of gIV-PLA(2) at Ser ( 505 ) caused by fMLP, thus inhibiting gIV-PLA(2) hydrolysis and production of AA and LTC ( 4 ) in eosinophils ... We find that PI3K is required for gIV-PLA(2) activation and hydrolytic production of AA in activated eosinophils ... We find that PI3K is required for gIV-PLA(2) activation and hydrolytic production of AA in activated eosinophils ... Our data suggest that this essential PI3K independently activates gIV-PLA(2) through a pathway that does not involve MAPK ... Our data suggest that this essential PI3K independently activates gIV-PLA(2) through a pathway that does not involve MAPK
Lu et al., Journal of molecular signaling 2010 : Overexpressing the adaptor protein SH2B1ß enhanced H2O2 induced PI3K-AKT and MEK-ERK1/2 signaling, reduced nucleus localized FoxOs and the expression of a pro-apoptotic gene, FasL
Wang et al., Gastroenterology 2001 : The PI3K inhibitor, wortmannin, in combination with sodium butyrate, synergistically induced IAP and sucrase enzyme activities and IAP messenger RNA levels in a time- and dose dependent fashion
Mantuano et al., J Neurosci 2011 : In cultured SCs, the PKR-like ER kinase target eIF2a was phosphorylated and CHOP was induced by ( 1 ) inhibiting PI3K , ( 2 ) treating the cells with tumor necrosis factor-a (TNF-a), or ( 3 ) genetic silencing of LRP1
Nagoshi et al., J Clin Invest 2005 (Myocardial Reperfusion Injury) : Biochemical analyses demonstrated that chronic Akt activation induces feedback inhibition of PI3K activity through both proteasome dependent degradation of insulin receptor substrate-1 (IRS-1) and inhibition of transcription of IRS-1 as well as that of IRS-2
Kingham et al., J Cell Sci 2009 : By comparison, pharmacological inhibition or siRNA mediated knockdown of p110alpha had no effect on mESC self-renewal per se, but instead appeared to reduce proliferation, which was accompanied by inhibition of leukaemia inhibitory factor (LIF) and insulin induced PI3K signalling
Li et al., Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2008 (Hypoxia-Ischemia, Brain) : To investigate the expression of hypoxia inducible factor 1alpha ( HIF-1alpha ) protein and the activation of phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathway in neurons under hypoxia ischemia condition, and to elucidate the role of PI3K/Akt on HIF-1alpha regulation in the developing neurons after hypoxia ischemia brain damage ( HIBD )
Zhang et al., PloS one 2011 : In adult C57BL/6 mice, acute ßAR stimulation induced significant increases in PI3K activity and activation of Akt and ERK1/2 in the heart, but not in lungs or livers ... In adult C57BL/6 mice, acute ßAR stimulation induced significant increases in PI3K activity and activation of Akt and ERK1/2 in the heart, but not in lungs or livers
Harris et al., Eur J Immunol 2012 : In this study, we reveal a novel role for PI3K? in the regulation of the pro-inflammatory cytokine IL-17 ... Loss of PI3K? or expression of a catalytically inactive mutant of PI3K? in mice led to increased IL-17 production both in vitro and in vivo in response to various stimuli
Woods et al., Neuro Oncol 2002 (Astrocytoma...) : Phosphatidylinositol 3'-kinase and MAPK/ERK kinase 1/2 differentially regulate expression of vascular endothelial growth factor in human malignant astrocytoma cells
Vigorito et al., Cell Signal 2006 : Synergistic activation of PKD by the B cell antigen receptor and CD19 requires PI3K , Vav1 and PLCgamma ... Synergistic activation of PKD by the B cell antigen receptor and CD19 requires PI3K , Vav1 and PLCgamma ... We found that PKD activation upon BCR engagement or coligation of the BCR with CD19 is entirely dependent on PI3K and PLCgamma but differ in the requirement for Vav proteins ... We found that PKD activation upon BCR engagement or coligation of the BCR with CD19 is entirely dependent on PI3K and PLCgamma but differ in the requirement for Vav proteins ... These findings show that Vav proteins and PI3K regulation of PLCgamma contributes to the activation of PKD in response to BCR and or CD19 cross linking ... These findings show that Vav proteins and PI3K regulation of PLCgamma contributes to the activation of PKD in response to BCR and or CD19 cross linking
Park et al., Cell Signal 2009 : In this study, we found that TRAIL inhibited PI3K/Akt dependent FoxO phosphorylation and relocated FoxO proteins into the nucleus from the cytosol in activated human hepatic stellate LX-2 cells
Xie et al., Prostate 2004 (Disease Progression...) : Regulation of interleukin-6 mediated PI3K activation and neuroendocrine differentiation by androgen signaling in prostate cancer LNCaP cells ... Addition of androgen blocks IL-6 mediated PI3K activation and NE differentiation in LNCaP cells ... In addition, androgen treatment enhances the interaction between AR and gp130, interrupts the IL-6 induced gp130 mediated PI3K activation, which may lead to inhibition of IL-6 mediated NE differentiation in LNCaP cells
Bertram et al., Cancer Res 2006 (Prostatic Neoplasms) : Here we report that apoptotic cell death of PTEN-deficient LNCaP and PC3 prostate cancer cells induced by the PI3K inhibitor LY294002 can be abrogated by disrupting Fas/Fas ligand (FasL) interactions with recombinant Fas : Fc fusion protein or FasL neutralizing antibody ( Nok-1 ), or by expressing dominant negative Fas associated death domain
Dijkers et al., Mol Cell Biol 2000 : This transcriptional regulation occurs through modulation of the forkhead transcription factor FKHR-L1, and IL-3 inhibited FKHR-L1 activity in a PI3K dependent manner ... Taken together, these observations indicate that inhibition of p27 ( KIP1 ) transcription through PI3K induced FKHR-L1 phosphorylation provides a novel mechanism of regulating cytokine mediated survival and proliferation
Ufer et al., Br J Pharmacol 2009 : Beta(3)-adrenoceptor treatment activated Akt although PI3K was not involved in beta(3)-adrenoceptor up-regulation
Kivens et al., Mol Cell Biol 1998 : Mutation of the proline residues in the K-G-P-P-L-P motif to alanines prevented CD2 mediated activation of integrin function and PI 3-K activity but not mitogen activated protein ( MAP ) kinase activity
Kim et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2011 : Thrombin induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells
Shafrir et al., Diabetes Metab Res Rev 2001 (Diabetes Mellitus, Experimental...) : Although evidence has been provided that the insulin receptor is activated, the possibility exists that cytosolic non-receptor tyrosine kinase, direct phosphorylation of IRS-1 and activation of PI3-K , leading to GLUT4 translocation, are involved
Tiwari et al., J Gen Virol 2010 : We also show that inhibition of PI3K signalling also affected RhoA activation required for HSV-1 entry into certain cell types
Huang et al., J Neurochem 2004 (Synaptic Transmission) : These results suggest that a PI3K/PKC dependent insulin signaling, which controls postsynaptic surface AMPA receptor numbers through PP-independent endocytosis, may be a major expression mechanism of insulin-LTD in hippocampal CA1 neurons
Bullone et al., J Vet Intern Med 2013 (Horse Diseases...) : PI3K and MAPKs regulate neutrophil migration toward the airways in heaves
Vagima et al., J Clin Invest 2009 : Treatment with G-CSF further increased MT1-MMP and decreased RECK expression in human and murine hematopoietic cells in a PI3K/Akt dependent manner, resulting in elevated MT1-MMP activity ... Treatment with G-CSF further increased MT1-MMP and decreased RECK expression in human and murine hematopoietic cells in a PI3K/Akt dependent manner, resulting in elevated MT1-MMP activity
Ueki et al., Glia 2013 : Interestingly, BMP/Smad1/5/8 activation downstream of PI3K/AKT signaling was also necessary for robust Müller glial proliferation, though activation of BMP/Smad1/5/8 signaling alone failed to stimulate their proliferation ... Interestingly, BMP/Smad1/5/8 activation downstream of PI3K/AKT signaling was also necessary for robust Müller glial proliferation, though activation of BMP/Smad1/5/8 signaling alone failed to stimulate their proliferation ... Interestingly, BMP/Smad1/5/8 activation downstream of PI3K/AKT signaling was also necessary for robust Müller glial proliferation, though activation of BMP/Smad1/5/8 signaling alone failed to stimulate their proliferation
Sánchez-Martín et al., J Biol Chem 2004 : We have identified Vav, a guanine nucleotide exchange factor for Rac-1, and PI3K/Akt, as regulators of the activation and inactivation phases of the activity of Rac-1, respectively, in the context of LFA-1 signaling based on the following experimental evidence : ( i ) LFA-1 induced activation of Vav and PI3K/Akt with kinetics consistent with a regulatory role for these molecules on Rac-1, ( ii ) overexpression of a constitutively active Vav mutant induces activation of Rac independently of LFA-1 stimulation whereas overexpression of a dominant negative Vav mutant blocks LFA-1 mediated Rac activation, ( iii ) pharmacological inhibition of PI3K/Akt prevented the fall in the activity of Rac-1 after its initial activation but had no effect on Vav activity, and ( iv ) overexpression of a dominant negative or a constitutively active Akt-1 induced or inhibited, respectively, Rac-1 activity
Hill et al., J Clin Invest 2008 (Body Weight) : Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice ... It has been proposed that leptin action requires PI3K activity
Maitra et al., J Immunol 2012 : Unlike high-dose LPS, low-dose LPS does not induce robust activation of NF-?B, MAPKs, PI3K , or anti-inflammatory mediators
Lu et al., Microvasc Res 2009 (Lung Neoplasms...) : Furthermore, lenalidomide inhibited VEGF induced PI3K-Akt pathway signaling, which is known to regulate adherens junction formation
Craddock et al., J Biol Chem 1999 : Using regulated expression of dominant negative p85 ( Deltap85 ) in stably transfected IL-3 dependent BaF/3 cells, we have specifically investigated the role of class IA PI3K in IL-3 signaling
Jin et al., PloS one 2013 : Moreover, sCD40L strongly stimulates neutrophil oxidative burst via CD40 dependent activation of PI3K/NF-KB , but independent of Mac-1 and PKC?
Hale et al., Proc Natl Acad Sci U S A 2010 : Overall, these data suggest that both direct binding of NS1 to p85beta ( resulting in repositioning of the N-terminal SH2 domain ) and possible NS1 : p110 contacts contribute to PI3K activation ... Overall, these data suggest that both direct binding of NS1 to p85beta ( resulting in repositioning of the N-terminal SH2 domain ) and possible NS1 : p110 contacts contribute to PI3K activation
Sharma et al., Mol Cell Biol 1997 : Thus, interference with the IRS-1-IR interaction inhibits insulin stimulated IRS-1 and Shc phosphorylation, PI 3-K enzymatic activity, p70s6k activation, MAPK phosphorylation and cell cycle progression ... Thus, interference with the IRS-1-IR interaction inhibits insulin stimulated IRS-1 and Shc phosphorylation, PI 3-K enzymatic activity, p70s6k activation, MAPK phosphorylation and cell cycle progression
Westra et al., Ann N Y Acad Sci 2009 (Arthritis, Rheumatoid) : Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed ... Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed
Seshadri et al., Proc Natl Acad Sci U S A 2010 : Receptors ErbB2 and ErbB3, but not ErbB4 , are responsible for transducing this effect, and PI3K/Akt signaling is also required
Staples et al., J Allergy Clin Immunol 2012 (Asthma) : The PI3K inhibitor LY294002 inhibited basal CCL17 release from BAL cells and IL-4 stimulated release from MDMs
Li et al., Int J Biochem Cell Biol 2005 (MAP Kinase Signaling System) : Here we tested the hypothesis that PI3K/Akt mediated inactivation of GSK-3beta and activation of mTOR contribute to the anabolic effects of IGF-I in dexamethasone treated myotubes ... Our results suggest that PI3K/Akt mediated inactivation of GSK-3beta and activation of mTOR contribute to the anabolic effects of IGF-I in dexamethasone treated myotubes
Wang et al., J Cell Physiol 2011 : Taken together, these results suggested that in astrocytes, activation of NF-?B by ET ( B ) -dependent c-Src, PI3K/Akt , and p42/p44 MAPK signalings is necessary for ET-1 induced iNOS gene up-regulation
Su et al., Life Sci 2010 (Adenocarcinoma...) : Immunoblot showed that NFD inhibited EGFR phosphorylation and the activation of PI3K/Akt , downstream molecules of EGFR pathway, in A549 cells
Li et al., Gastroenterology 2004 : EGF increased PI 3-K and active Akt2 in ileal BBM at the same time that it increased PI 3-K dependent trafficking of NHE3 to BBM and stimulation of Na absorption
Krugmann et al., Curr Biol 2004 : We found no evidence for direct regulation of ARAP3 's Rho GAP activity by PtdIns ( 3,4,5 ) P ( 3 ) in vitro, but PI3K activity was required for activation by Rap in a cellular context, suggesting that PtdIns ( 3,4,5 ) P ( 3 ) -dependent translocation of ARAP3 to the plasma membrane may be required for further activation by Rap
Nielsen-Preiss et al., Endocrinology 2007 : In addition, PI3K inhibition in GnRH neuronal cells diminished Gas6 induced Rac activation
Wu et al., Kidney Int 2006 : P-PI3-K blockade downregulated the CTGF stimulated expression of P-PI3-K , P-Akt, and NF-kappaB but not P-p42/44 MAPK, and partially decreased the release of the above chemokines ... LXA ( 4 ) dose-dependently inhibited the CTGF stimulated mRNA expression and protein release of the above chemokines, and the expression of P-p42/44MAPK, P-PI3-K , P-Akt, and NF-kappaB
Song et al., Journal of neuroinflammation 2012 (Neuroblastoma) : We also found that Src/PI3K/Akt inhibitors prevented LLLT stimulated Akt ( Ser473 and Thr308 ) phosphorylation and blocked Rac1 activity and actin based microglial phagocytosis, indicating the activation of Src/PI3K/Akt/Rac1 signaling pathway ... We also found that Src/PI3K/Akt inhibitors prevented LLLT stimulated Akt ( Ser473 and Thr308 ) phosphorylation and blocked Rac1 activity and actin based microglial phagocytosis, indicating the activation of Src/PI3K/Akt/Rac1 signaling pathway
Liu et al., Ann Hematol 2010 (Leukemia, Myeloid) : Inactivation of PI3k/Akt signaling pathway and activation of caspase-3 are involved in tanshinone I-induced apoptosis in myeloid leukemia cells in vitro
Molnarfi et al., J Immunol 2005 : The use of cycloheximide and actinomycin D shows that sIL-1Ra was an immediate early gene induced by IFN-beta and that PI3K was controlling sIL-1Ra gene transcription ... Although both inhibitors of PI3K and MEK1 diminished the Ser ( 727 ) phosphorylation of STAT1 induced by IFN-beta, only Ly294002 inhibited sIL-1Ra production
Nevzorova et al., Br J Pharmacol 2006 : The PI3K inhibitor, LY294002 ( 1 microM ), inhibited insulin- ( 174.9+/-5.9 to 142.7+/-2.7 % ) and zinterol- ( 166.9+/-7.6 to 141.1+/-8.1 % ) but not 8 Br-cAMP stimulated GU
Simoncini et al., Steroids 2004 : For instance, estrogens and glucocorticoids trigger rapid vasodilatation due to rapid induction of nitric oxide synthesis in endothelial cells via the estrogen receptor dependent activation of MAPK and PI3K , leading to relevant pathophysiological consequences, in vitro and in vivo
Bonaccorsi et al., Steroids 2004 (Neoplasm Invasiveness...) : In addition, EGF stimulated PI3K activity, a key signalling pathway for invasion of these cells, was decreased in PC3-AR cells and further reduced by treatment with R1881, indicating decreased functionality of EGFR
Reyes-Reyes et al., Biochem J 2002 : However, PI-3K was required for beta1 integrin-, but not beta2 integrin-, mediated NF-kappaB activation
Jiang et al., J Immunol 2001 : Whereas a calcineurin dependent pathway was essential for Bik mRNA induction, both the phosphatidylinositol 3-kinase (PI3K)- and the calcineurin dependent pathways were required for the sustained production of Bik protein
Wunderlich et al., Exp Dermatol 2008 : Our findings indicate the involvement of PI3K in UVB mediated HIF-1alpha upregulation
Saal et al., Proc Natl Acad Sci U S A 2007 (Carcinoma) : Taking advantage of the observation that loss of PTEN , the negative regulator of PI3K , results in robust activation of this pathway, we developed and validated a microarray gene expression signature for immunohistochemistry ( IHC ) -detectable PTEN loss in breast cancer ( BC )
Bai et al., Int J Cancer 2009 (Cell Transformation, Neoplastic) : The data further support the conclusion that NFkappaB activity is essential for PI3K- and Akt induced oncogenic transformation
Hashimoto et al., Int J Mol Med 2005 : This APJ dependent activation of Akt/PKB was significantly inhibited by the pretreatment of pertussis toxin ( PTx ) and a PI3K inhibitor, LY29004 ... This APJ dependent activation of Akt/PKB was significantly inhibited by the pretreatment of pertussis toxin ( PTx ) and a PI3K inhibitor, LY29004
Jutooru et al., Mol Carcinog 2009 (Pancreatic Neoplasms) : Induction of NAG-1 in Panc28 cells was p38-MAPK- and PI3-K dependent but Egr-1 independent, whereas induction in Panc1 cells was associated with activation of p38-MAPK, PI3-K, and p42-MAPK and was only partially Egr-1 dependent
Kruszynska et al., J Clin Endocrinol Metab 2002 (Insulin Resistance) : The insulin induced increase in IRS-1 associated PI3K activity was impaired by elevated NEFA, so that activity at the end of the clamps with Intralipid was 35 % lower than in the control clamps ( P < 0.05 )
Folgiero et al., Cancer Res 2007 (Breast Neoplasms...) : Given that ErbB-2 can activate PI3K only when it heterodimerizes with other members of the epidermal growth factor receptor family, these data imply that other receptors cooperate in this process
Huang et al., Int J Cardiol 2009 (Atherosclerosis) : Metformin inhibits TNF-alpha induced IkappaB kinase phosphorylation, IkappaB-alpha degradation and IL-6 production in endothelial cells through PI3K dependent AMPK phosphorylation ... This effect was related to PI3K dependent AMPK phosphorylation
Manna et al., J Biol Chem 2011 : The PIP3 increase is mediated by PI3K activation and inhibition of PTEN but not of SHIP2 ... The PIP3 increase is mediated by PI3K activation and inhibition of PTEN but not of SHIP2 ... The PIP3 increase is mediated by PI3K activation and inhibition of PTEN but not of SHIP2
Chan et al., J Biol Chem 2008 (MAP Kinase Signaling System) : PAK is regulated by PI3K , PIX, CDC42, and PP2Calpha and mediates focal adhesion turnover in the hyperosmotic stress induced p38 pathway
Kin et al., J Immunol 2006 : In this study, we show that CD86 stimulation on an activated B cell increases the activity of PI3K and the phosphorylation of phosphoinositide dependent kinase 1, Akt, and IkappaB kinase alphabeta
Gao et al., Toxicol Appl Pharmacol 2010 : A constitutive level of PI3K dependent Akt phosphorylation remained unchanged by Ni and/or MALP-2 exposure
Lasala et al., Am J Physiol Endocrinol Metab 2011 : Blocking PKA abolished the effect of cAMP on Sox9, Sf1, and Gata4 expression, inhibiting PI3K/PKB impaired the effect on Sf1 and Gata4, and reducing MEK1/2 and p38 MAPK activities curtailed Gata4 increase ... Blocking PKA abolished the effect of cAMP on Sox9, Sf1, and Gata4 expression, inhibiting PI3K/PKB impaired the effect on Sf1 and Gata4, and reducing MEK1/2 and p38 MAPK activities curtailed Gata4 increase ... Blocking PKA abolished the effect of cAMP on Sox9, Sf1, and Gata4 expression, inhibiting PI3K/PKB impaired the effect on Sf1 and Gata4 , and reducing MEK1/2 and p38 MAPK activities curtailed Gata4 increase ... Blocking PKA abolished the effect of cAMP on Sox9, Sf1, and Gata4 expression, inhibiting PI3K/PKB impaired the effect on Sf1 and Gata4, and reducing MEK1/2 and p38 MAPK activities curtailed Gata4 increase
Alcázar et al., Blood 2009 : Moreover, deletion of p85beta impaired CD28 induced intracellular events, including c-CBL and CBL-b down-regulation as well as PI3K pathway activation
Huang et al., Acta Pharmacol Sin 2005 (Breast Neoplasms...) : The PI3K inhibitors wortmannin and Ly294002, but not rapamycin, completely inhibited the phosphorylation of Akt and PRAS40
Jiang et al., Cancer Res 2011 (Colonic Neoplasms) : PRL-3 activation stimulated PI3K/AKT signaling that caused resistance to stress induced apoptosis
Bhaskar et al., Molecular neurodegeneration 2009 : Finally, our results also demonstrate that Abeta oligomer treated neurons exhibit elevated levels of activated Akt and mTOR ( mammalian Target Of Rapamycin ) and that PI3K , Akt or mTOR inhibitors blocked Abeta oligomer induced neuronal CCEs ... Finally, our results also demonstrate that Abeta oligomer treated neurons exhibit elevated levels of activated Akt and mTOR ( mammalian Target Of Rapamycin ) and that PI3K , Akt or mTOR inhibitors blocked Abeta oligomer induced neuronal CCEs
Haga et al., Endothelium : journal of endothelial cell research 2003 : Phosphorylation of Akt in EC exposed to SS or CS was time dependent but with maximal stimulation at 30 min ( SS ) or 5 min ( CS ) ; SS- or CS-induced Akt phosphorylation was inhibited in the presence of PI3K inhibition
Lee et al., Mol Cells 2008 (Breast Neoplasms) : In contrast, the effect of the PI3K/Akt inhibitor on ER alpha was blocked in cells that were treated with LY294002 in the presence of the proteasome inhibitors
Cheng et al., PloS one 2012 (Cystadenocarcinoma, Serous...) : The PI3K inhibitor LY294002 had similar effects, but it could not block the EGF induced up-regulation of N-cadherin and ZEB1 ... The PI3K inhibitor LY294002 had similar effects, but it could not block the EGF induced up-regulation of N-cadherin and ZEB1
von Gise et al., Mol Cell Biol 2001 : The survival effect of activated MEK in 32D cells is achieved by both MEK- and PI3K dependent mechanisms and results in the activation of PI3K and in the phosphorylation of AKT
Hwang et al., Carcinogenesis 2004 (Prostatic Neoplasms) : Phosphatidylinositol-3 kinase/Akt/mammalian target of rapamycin signaling was also involved in vanadate induced HIF-1alpha expression, but it was independent of AMPK signaling pathway
Uttarwar et al., American journal of physiology. Renal physiology 2012 (Diabetes Mellitus, Experimental...) : Thus HG-induced SREBP-1 activation requires EGFR/PI3K/RhoA signaling and SCAP mediated transport to the Golgi for its proteolytic cleavage
Catarzi et al., Biochim Biophys Acta 2005 : Therefore, we suggest a redox circuit whereby, upon PDGF stimulation, PKC, PI3K and NADPH oxidase activity contribute to complete c-Src kinase activation, thus promoting maximal phosphorylation and activation of PDGFr tyrosine phosphorylation
Hallmann et al., J Biol Chem 2003 (MAP Kinase Signaling System) : Although, IGF-1 stimulated PI3K activity associated with insulin receptor substrates was unaltered in all cell lines, p85alpha-null ES cells showed diminished protein kinase B activation despite increased PI3K activity associated with the p85beta subunit
Abdelmegeed et al., J Pharmacol Exp Ther 2005 : Heterogeneous nuclear RNA analysis revealed that AA suppressed CYP2E1 gene transcription by approximately 50 % and that inhibition of PI 3-K and PKC diminished this AA-mediated effect on transcription
Ray et al., Int J Oncol 2007 (Breast Neoplasms...) : MCF-7 cells expressed higher levels of Ob-Rb, Jak2, PI3K , Stat3 and p-Stat3 in a dose dependent manner to 50 ng/ml at 24 h ; and IGF-IRalpha increased at 24 h. Cyclin D1 and Cox-2 levels increased with leptin treatment
Meroni et al., J Endocrinol 2002 : Taken together, these results indicate that FSH increases P-PKB levels in a PI3K dependent and PKA independent manner in rat Sertoli cells
Liang et al., J Biol Chem 2009 (Breast Neoplasms) : Because a substantial percentage of breast tumors have RGS16 mutations and reduced RGS16 protein expression, we investigated the link between regulation of PI3K activity by RGS16 and breast cancer cell growth ... These results suggest that the loss of RGS16 in some breast tumors enhances PI3K signaling elicited by growth factors and thereby promotes proliferation and TKI evasion downstream of HER activation
Fang et al., Mol Cell Biol 2002 : PI3K inhibitors block insulin- but not LPA induced GSK-3 phosphorylation
Dessauer et al., Ann N Y Acad Sci 2005 : We have described a novel bifurcated pathway by which relaxin stimulates Gs alpha and PI3K/PKCzeta leading to increased cAMP production and increased VEGF gene expression
Kops et al., Mol Cell Biol 2002 (Colonic Neoplasms) : As for the activity of the Forkheads, we also show that protein levels of p130 are controlled by endogenous PI3K/PKB signaling upon cell cycle reentry
Jung et al., Invest Ophthalmol Vis Sci 2007 : Upregulation of TGF-beta induced tissue transglutaminase expression by PI3K-Akt pathway activation in human subconjunctival fibroblasts
Pan et al., Biochem Pharmacol 2006 (Cocarcinogenesis...) : We found that acacetin also inhibited LPS induced activation of PI3K/Akt and p44/42, but not p38 MAPK ... Taken together, these results show that acacetin down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NF kappa B by interfering with the activation PI3K/Akt/IKK and MAPK, suggesting that acacetin is a functionally novel agent capable of preventing inflammation associated tumorigenesis ... Taken together, these results show that acacetin down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NF kappa B by interfering with the activation PI3K/Akt/IKK and MAPK, suggesting that acacetin is a functionally novel agent capable of preventing inflammation associated tumorigenesis
Zhang et al., Biol Pharm Bull 2010 (Insulin Resistance) : Moreover, 7-O-MA stimulated the reactivation of insulin mediated phosphorylation of phosphatidylinositol 3-kinase (PI3K) linked protein kinase B ( Akt/PKB ) and adenosine 5'-monophosphate activated protein kinase ( AMPK ) in high glucose induced, insulin-resistant HepG2 cells, and this effect was blocked by either LY294002, a PI3K inhibitor, or compound C, an AMPK inhibitor
Han et al., Toxicol Appl Pharmacol 2008 : Phosphatidylinositol 3 (PI3)-kinase , its downstream signaling molecule, Akt, and mitogen activated protein kinases ( MAPK ) were also significantly activated by the o, p'-DDT induced AP-1 and CRE activation
Ma et al., Biochim Biophys Acta 2013 (Brain Ischemia...) : We observed that overexpression of HSPA12B activated PI3K/Akt signaling and suppressed JNK and p38 activation following cerebral I/R. Importantly, pharmacological inhibition of PI3K/Akt signaling abrogated the protection against cerebral I/R injury in Tg mice
Lu et al., Diabetes 2008 (Diabetes Mellitus, Type 2) : Inhibition of PI3K with wortmannin or suppression of Akt activation with dominant negative Akt inhibited FGF2 expression
Larizza et al., Leukemia & lymphoma 2005 (Acute Disease...) : PI3K dependent activation of AKT and STAT activation was observed in Kasumi-1 cells ... PI3K dependent activation of AKT and STAT activation was observed in Kasumi-1 cells
Zipper et al., Toxicological sciences : an official journal of the Society of Toxicology 2003 (Translocation, Genetic) : Pyrrolidine dithiocarbamate ( PDTC ) induction of the human glutamate cysteine ligase modulatory ( GCLM ) gene is dependent on activation of the mitogen activated protein kinases ( MAPKs ) extracellular regulated kinase (Erk) and p38, and is not affected by protein kinase C ( PKC ) or PI3K inhibitors
Park et al., Toxicol Appl Pharmacol 2006 (Lymphoma) : In contrast, the enhanced AP-1 DNA binding activities and p38 MAPK phosphorylation were significantly suppressed by specific inhibitors for PKC and p38 MAPK, but not by PI3-K inhibitors ... In contrast, the enhanced AP-1 DNA binding activities and p38 MAPK phosphorylation were significantly suppressed by specific inhibitors for PKC and p38 MAPK, but not by PI3-K inhibitors ... In contrast, the enhanced AP-1 DNA binding activities and p38 MAPK phosphorylation were significantly suppressed by specific inhibitors for PKC and p38 MAPK, but not by PI3-K inhibitors
Kim et al., Exp Mol Med 2009 : A chemical inhibitor of MEK1/2 or PI3K reduced phosphorylation of ERK or Akt, respectively, and also inhibited CSE mediated MMP-9 induction ... A chemical inhibitor of MEK1/2 or PI3K reduced phosphorylation of ERK or Akt , respectively, and also inhibited CSE mediated MMP-9 induction ... A chemical inhibitor of MEK1/2 or PI3K reduced phosphorylation of ERK or Akt, respectively, and also inhibited CSE mediated MMP-9 induction
Meng et al., Br J Cancer 2009 (Adenocarcinoma...) : In addition, the Src inhibitor, PP2, blocked the phosphorylation of FAK, ERK1/2, PI3K , and Akt ... The PI3K inhibitor, LY294002, further blocked the expression of MMP9 and RhoA ... The PI3K inhibitor, LY294002, further blocked the expression of MMP9 and RhoA
Stevens et al., Journal of thrombosis and haemostasis : JTH 2004 : Furthermore, ILK activity is dually regulated by PI3K and PKC in thrombin stimulated platelets and regulated by PI3K in collagen stimulated cells
Kajanne et al., Int J Oncol 2009 (Prostatic Neoplasms) : Here, we show that constitutive AP-1 activity in prostate cancer cells is dependent on the activities of EGF-R and PI3K ... Together, the findings show that AP-1 activity in prostate cancer cells mediates EGF-R and PI3K signalling, is essential for their proliferation, and confers protection against radiation induced cell death
Tybulewicz , Eur J Immunol 2004 (Second Messenger Systems) : Unexpectedly, they show that while the BCR induced phosphorylation of the PI3K dependent kinase Akt is reduced in p85alpha-deficient cells, the phosphorylation of two downstream targets of Akt -- FOXO1 and ribosomal protein S6 -- is largely unaffected
Moshal et al., J Cell Physiol 2008 (Hyperhomocysteinemia) : The result suggested that Hcy downregulated CYP2J2 protein expression and dephosphorylated PI3K dependent AKT signal
Webb et al., J Immunol 2005 : This work demonstrates that PI3K activity is critical for T cell development and depends on the combined function of p110gamma and p110delta ... This work demonstrates that PI3K activity is critical for T cell development and depends on the combined function of p110gamma and p110delta
Mehta et al., Growth Factors 2007 (MAP Kinase Signaling System) : HB-EGF- and EGF induced HUVEC migration and capillary tube formation were dependent upon activation of PI3K , MAPK and eNOS ... HB-EGF- and EGF induced HUVEC migration and capillary tube formation were dependent upon activation of PI3K , MAPK and eNOS
Wang et al., J Biol Chem 2010 : Shear induced interleukin-6 synthesis in chondrocytes : roles of E prostanoid ( EP ) 2 and EP3 in cAMP/protein kinase A- and PI3-K/Akt dependent NF-kappaB activation ... Selective knockdown of EP2 or ectopic expression of EP3 blocks PKA- and PI3-K/Akt dependent p65 activation and markedly diminishes shear induced IL-6 expression
Smyth et al., J Immunol 2006 (Inflammation) : Furthermore, inhibition of PI3K by use of LY294002 reduces expression of IL-6 at both the mRNA and protein level in murine fibroblasts, and we suggest that PI3K is required for activation of PKCdelta
Lau et al., J Immunol 2008 : We postulated that Syk regulates PI3K activation and HRV endocytosis in the airway epithelium ... HRV induced PI3K activation was dependent on Syk ; Syk knockdown by small interfering RNA significantly decreased phosphorylation of the PI3K substrate Akt
Lilja et al., J Neurosci Res 2007 (Neuroblastoma) : By blocking phosphatidylinositol-3-kinase [ PI(3)K ] or mitogen activated protein kinase ( MAPK ) signaling, we concluded that the increase in total TRPV1 protein content induced by IGF-I was controlled by PI(3)K signaling, whereas insulin seemed to regulate TRPV1 protein expression via both PI(3)K and MAPK pathways
Tabassam et al., Helicobacter 2012 (Helicobacter Infections) : Inhibition of PI3K or Akt kinase activity reduced FoxO1/3a phosphorylation ... Infection with oipA mutants reduced PI3K/Akt activation and inhibited FoxO1/3a phosphorylation, whereas infection with cag PAI mutants reduced PI3K/Akt activity but did not inhibit FoxO1/3a activation
Scholz et al., J Invest Surg 2004 : We further showed that CD29/TNF-alpha mediated effects involved PI3K and tyrosine kinase dependent signaling via MAPK but were independent of nuclear transcription factor (NF)-kappaB activity ... We further showed that CD29/TNF-alpha mediated effects involved PI3K and tyrosine kinase dependent signaling via MAPK but were independent of nuclear transcription factor (NF)-kappaB activity
Darido et al., Cancer Cell 2011 (Carcinoma, Squamous Cell...) : Deletion of Grhl3 in adult epidermis evokes loss of expression of PTEN, a direct GRHL3 target, resulting in aggressive SCC induced by activation of PI3K/AKT/mTOR signaling
Xie et al., Mol Biol Cell 2005 : PI3K inhibitors blocked calcium activation of PLC-gamma1 as well as the induction of keratinocyte differentiation markers involucrin and transglutaminase ... PI3K inhibitors blocked calcium activation of PLC-gamma1 as well as the induction of keratinocyte differentiation markers involucrin and transglutaminase ... These data indicate that calcium activates PLC-gamma1 via increased PIP3 formation mediated by c-src- and fyn activated PI3K
Desai et al., J Biol Chem 2010 : Expression of constitutively active PI3K stimulated translocation of Tiam1 to the membrane, increased Rac1 activity, and increased wound healing of airway epithelial cells ... Expression of constitutively active PI3K stimulated translocation of Tiam1 to the membrane, increased Rac1 activity, and increased wound healing of airway epithelial cells
Comb et al., Mol Cell 2012 (Starvation) : Cells expressing p85 S690A or inhibited for IKK activity exhibit increased Akt activity following cell starvation, demonstrating that p85 phosphorylation is required for starvation induced PI3K feedback inhibition ... Finally, leucine deprivation is shown to be necessary and sufficient for starvation induced, IKK mediated p85 phosphorylation and PI3K feedback inhibition
So et al., Mol Immunol 2007 (MAP Kinase Signaling System) : In cytokine producing Jurkat T cells, we have found that IL-4 induces activation of Erk and Akt, and the IL-4 induced STAT6 activity is suppressed by inhibitors of Erk and PI3K
Seo et al., PloS one 2013 : MS-enhanced MMP-2 expression and activity were inhibited by molecular inhibition of Akt using Akt siRNA as well as by PI3K/Akt inhibitors, LY293002 and AI, but not by MAPK inhibitors such as PD98059, SP600125 and SB203580
Suzuki et al., Biochem Biophys Res Commun 2000 (Breast Neoplasms) : While the IGF-I induced activation of PKB/Akt was inhibited by PI3-K inhibitor LY294002 but not by MEK inhibitor PD98059, the activation of both MEK and ERK by IGF-I was inhibited by both ... While the IGF-I induced activation of PKB/Akt was inhibited by PI3-K inhibitor LY294002 but not by MEK inhibitor PD98059, the activation of both MEK and ERK by IGF-I was inhibited by both
Feliers et al., Kidney Int 2005 (MAP Kinase Signaling System) : VEGF stimulated Akt phosphorylation in a PI3K dependent manner
Corcione et al., PloS one 2009 :