◀ Back to PIK3R1
CD28 — PIK3R1
Pathways - manually collected, often from reviews:
-
BioCarta ras-independent pathway in nk cell-mediated cytotoxicity:
AR ligand/Activating receptors complex (CD28_FCGR3B_FCGR3A_CD2)
→
PI3K complex (PIK3CA-PIK3R1)
(modification, activates)
-
BioCarta the co-stimulatory signal during t-cell activation:
CD28/CD80 complex (CD80-CD28)
→
PI3K complex (PIK3CA-PIK3R1)
(modification, activates)
-
KEGG T cell receptor signaling pathway:
CD28
→
PIK3CA/PIK3CB/PIK3CD/PIK3CG/PIK3R1/PIK3R2/PIK3R3/PIK3R5
(protein-protein, activation)
-
KEGG Measles:
CD28
→
PIK3CA/PIK3CB/PIK3CD/PIK3CG/PIK3R1/PIK3R2/PIK3R3/PIK3R5
(protein-protein, activation)
-
Reactome Reaction:
CD28
→
PIK3R1
(indirect_complex)
Kane et al., Immunol Rev 2003*, Pagès et al., J Biol Chem 1996*
-
Reactome Reaction:
CD28
→
PIK3R1
(reaction)
Kane et al., Immunol Rev 2003*, Pagès et al., J Biol Chem 1996*
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
PIK3R1
—
CD28
(physical association, affinity chromatography technology)
Alcázar et al., Blood 2009*
-
IRef Biogrid Interaction:
PIK3R1
—
CD28
(physical association, affinity chromatography technology)
Pagès et al., J Biol Chem 1996*
-
IRef Biogrid Interaction:
PIK3R1
—
CD28
(direct interaction, unspecified method)
Pagès et al., J Biol Chem 1996*
-
IRef Innatedb Interaction:
CD28
—
PIK3R1
(unknown, -)
Fang et al., Nat Immunol 2001*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, competition binding)
Prasad et al., Proc Natl Acad Sci U S A 1994*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Pagès et al., Nature 1994*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Kim et al., J Biol Chem 1998*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Ellis et al., J Immunol 2000*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, pull down)
Ellis et al., J Immunol 2000*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Raab et al., Proc Natl Acad Sci U S A 1995
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Cai et al., Immunity 1995*
-
IRef Intact Interaction:
CD28
—
PIK3R1
(association, coimmunoprecipitation)
Schneider et al., J Exp Med 1995*
-
IRef Ophid Interaction:
CD28
—
PIK3R1
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Phu et al., J Leukoc Biol 2001
:
These results support the conclusion that
PI3-K activation is
involved in T-cell receptor,
CD28 , and IL-2 receptor signaling of CD8 ( + ) T cells
Bahlis et al., Blood 2007
(Multiple Myeloma) :
We have found that direct activation of myeloma cell
CD28 by anti-CD28 mAb alone
induces activation of
PI3K and NFkappaB, suppresses MM cell proliferation, and protects against serum starvation and dexamethasone ( dex ) -induced cell death
Nurieva et al., J Immunol 2007
:
We found that
CD28 and ICOS
regulate sustained
PI3K activity in primary T cells, which is required for NFATc1 up-regulation
Garçon et al., Blood 2008
:
CD28 provides T-cell costimulation and
enhances PI3K activity at the immune synapse independently of its capacity to interact with the p85/p110 heterodimer
Gogishvili et al., Eur J Immunol 2008
:
In p110delta ( D910A/D910A ) transgenic T cells, which are defective in
PI3K activation following
CD28 ligation, proliferation was comparable to that in wild-type cells
Alcázar et al., Blood 2009
:
Moreover, deletion of p85beta impaired
CD28 induced intracellular events, including c-CBL and CBL-b down-regulation as well as
PI3K pathway activation
Gigoux et al., Proc Natl Acad Sci U S A 2009
:
Although it is known that
CD28 mediated
PI3K activation is dispensable for GC reaction, the role of ICOS-driven PI3K signaling has not been defined
Muscolini et al., J Immunol 2013
:
Hence, PIP2 is a crucial substrate in driving the PI3K downstream signaling pathways, and PIP2 turnover may be an essential regulatory step to ensure the
activation of
PI3K following
CD28 engagement
Truitt et al., J Immunol 1995
(Second Messenger Systems) :
Stimulation of CD28 induces its association with phosphatidylinositol 3'-kinase (PI3-K), raising the possibility that
PI3-K plays a critical role in
CD28 signaling
Lu et al., Eur J Immunol 1995
:
Wortmannin added to cell cultures partially inhibits CD28 induced tyrosine phosphorylation of the putative p110 catalytic subunit of PI3-K, but does not block
CD28 induced association of the p85
PI3-K regulatory subunit with the CD28 receptor
Ghiotto-Ragueneau et al., Eur J Immunol 1996
:
We demonstrate in this report that both
CD28-B7.1 and CD28-B7.2 interactions
induce the association of
PI3-K to CD28 in the CD4 subpopulation, whereas it was barely detectable in CD8 cells
Lu et al., Eur J Immunol 1996
(Leukemia) :
Engagement of
CD28 caused the rapid receptor association and activation of
PI3-K but did not activate phospholipase C gamma ...
CD28 induced tyrosine phosphorylation and
PI3-K activation was independent of p56lck protein tyrosine kinase ( PTK ) activity ( previously reported to be associated with CD28 ) and was insensitive to inhibition by the PTK inhibitor herbimycin A ... Two structurally and mechanistically dissimilar inhibitors of PI3-K, wortmannin and 2- ( 4-morpholinyl ) -8-phenyl-4H-1-benzopyran-4-one ( LY294002 ) also failed to block
CD28 dependent tyrosine phosphorylation events or the association of
PI3-K with the CD28 receptor ... Although herbimycin A did not significantly block the observed
CD28 dependent tyrosine phosphorylation or
PI3-K activation, herbimycin did block CD28 dependent cytotoxicity in a dose dependent manner ... These data support a role for PI3-K activation in the CD28 dependent initiation of cytotoxic effector function and suggest that a herbimycin sensitive step ( s ) is either CD28 independent, resides within a PI3-K independent CD28 signaling pathway, or is downstream of
CD28 dependent
PI3-K activation
Lu et al., J Immunol 1998
:
Phosphatidylinositol 3-kinase is
required for
CD28 but not CD3 regulation of the TEC family tyrosine kinase EMT/ITK/TSK : functional and physical interaction of EMT with phosphatidylinositol 3-kinase ...
CD28 induced association of EMT with
PI3K also requires functional expression of LCK