◀ Back to LCK
CD3E — LCK
Pathways - manually collected, often from reviews:
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BioCarta the co-stimulatory signal during t-cell activation:
MHC II/TCR-CD3 complex (CD3G-HLA-DRB1-TRA@-CD3D-TRB@-HLA-DRA-CD3E-CD3Z)
→
LCK
(T cell activation, activates)
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BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
MHC class II complex (HLA-DRA-HLA-DRB1)
→
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
(modification, collaborate)
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BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
CD4
(modification, collaborate)
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BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
LCK
(modification, collaborate)
-
BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
TCR-CD3 complex (CD3Z-TRA@-TRB@-CD3G-CD3E-CD3D)
(modification, collaborate)
-
BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
LCK
→
TCR-CD3 complex (CD3Z-TRA@-TRB@-CD3G-CD3E-CD3D)
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
TCR-CD3/MHC II/CD4/LCK complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK)
→
ZAP70
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
TCR-CD3/MHC II/CD4/LCK complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK)
→
TCR-CD3/MHC II/CD4/LCK/ZAP70 complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
ZAP70
→
TCR-CD3/MHC II/CD4/LCK/ZAP70 complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
-
BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/ZAP70/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-ZAP70-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
(modification, collaborate)
-
BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/ZAP70/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-ZAP70-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
ZAP70
(modification, collaborate)
-
BioCarta activation of csk by camp-dependent protein kinase inhibits signaling through the t cell receptor:
CD3Z antigen zeta polypeptide/MHC class II alpha/CD4/LCK/CD3Z antigen zeta polypeptide/TCR-alpha/TCR-beta/CD3G antigen gamma polypeptide/CD3E antigen epsilon polypeptide/CD3E antigen epsilon polypeptide/CD3D antigen delta polypeptide/MHC IIb complex (CD3Z-HLA-DRA-CD4-LCK-TRA@-TRB@-CD3G-CD3E-CD3D-HLA-DRB1)
→
ZAP70
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
TCR-CD3/MHC II/CD4/LCK complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK)
→
LCK
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
TCR-CD3/MHC II/CD4/LCK complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK)
→
TCR-CD3/MHC II/CD4 complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
-
BioCarta t cell receptor signaling pathway:
LCK
→
TCR-CD3/MHC II/CD4 complex (TRA@-TRB@-CD3Z-CD3G-CD3E-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
PKC theta (PRKCQ)
(modification, activates)
Liu et al., J Biol Chem 2000, Bi et al., Nat Immunol 2001, Díaz-Flores et al., J Biol Chem 2003, Imboden et al., J Exp Med 1985
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
(modification, activates)
Liu et al., J Biol Chem 2000, Bi et al., Nat Immunol 2001, Díaz-Flores et al., J Biol Chem 2003, Imboden et al., J Exp Med 1985
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
CSK/PAG1 complex (CSK-PAG1)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
(modification, collaborate)
Brdicka et al., J Exp Med 2000
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
Csk (CSK)
(modification, activates)
Brdicka et al., J Exp Med 2000
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B)
→
LCK (LCK)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Anel et al., Eur J Immunol 1996
Evidence: mutant phenotype, assay, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Anel et al., Eur J Immunol 1996
Evidence: mutant phenotype, assay, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
B7 family/CD28 complex (CD28-CD80_CD86)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, activates)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Anel et al., Eur J Immunol 1996
Evidence: mutant phenotype, assay, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
LCK (LCK)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Anel et al., Eur J Immunol 1996
Evidence: mutant phenotype, assay, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
B7 family/CD28 complex (CD28-CD80_CD86)
(modification, collaborate)
Pelosi et al., J Biol Chem 1999, Iwashima et al., Science 1994, Chan et al., J Immunol 1994, van Oers et al., J Exp Med 1996, Tuosto et al., Eur J Immunol 1998
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
ZAP-70 (ZAP70)
(modification, collaborate)
Pelosi et al., J Biol Chem 1999, Iwashima et al., Science 1994, Chan et al., J Immunol 1994, van Oers et al., J Exp Med 1996, Tuosto et al., Eur J Immunol 1998
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Pelosi et al., J Biol Chem 1999, Iwashima et al., Science 1994, Chan et al., J Immunol 1994, van Oers et al., J Exp Med 1996, Tuosto et al., Eur J Immunol 1998
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
B7 family/CD28 complex (CD28-CD80_CD86)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, activates)
Pelosi et al., J Biol Chem 1999, Iwashima et al., Science 1994, Chan et al., J Immunol 1994, van Oers et al., J Exp Med 1996, Tuosto et al., Eur J Immunol 1998
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
ZAP-70 (ZAP70)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Pelosi et al., J Biol Chem 1999, Iwashima et al., Science 1994, Chan et al., J Immunol 1994, van Oers et al., J Exp Med 1996, Tuosto et al., Eur J Immunol 1998
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
SHC (SHC1)
(modification, activates)
Walk et al., Eur J Immunol 1998
Evidence: expresssion pattern
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
LAT/GRAP2/SLP76/VAV1 complex (LAT-GRAP2-LCP2-VAV1)
(modification, activates)
Reynolds et al., J Exp Med 2002, Fukazawa et al., J Biol Chem 1995, Exley et al., J Biol Chem 1994, Prasad et al., Mol Cell Biol 1993, Zhang et al., Cell 1998
Evidence: mutant phenotype, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
TCR/CD3/MHC II/CD4/LCK/CBL/Ubiquitin complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247-CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
CBL (CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/CBL/Ubiquitin complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247-CBL)
→
CBL (CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
B7 family/CD28 complex (CD28-CD80_CD86)
(modification, activates)
Woods et al., EMBO J 2001, Wilcox et al., J Biol Chem 2003, August et al., Proc Natl Acad Sci U S A 1994, Raab et al., Proc Natl Acad Sci U S A 1995, Gibson et al., J Biol Chem 1996, Heyeck et al., J Biol Chem 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
ITK (ITK)
(modification, activates)
Woods et al., EMBO J 2001, Wilcox et al., J Biol Chem 2003, August et al., Proc Natl Acad Sci U S A 1994, Raab et al., Proc Natl Acad Sci U S A 1995, Gibson et al., J Biol Chem 1996, Heyeck et al., J Biol Chem 1997
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
SHP2 (PTPN11)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, inhibits)
Kwon et al., EMBO J 2005, Han et al., Science 1998, Michel et al., J Biol Chem 1998
Evidence: mutant phenotype, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
Fyn (FYN)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, activates)
Kwon et al., EMBO J 2005, Han et al., Science 1998, Michel et al., J Biol Chem 1998
Evidence: mutant phenotype, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
CBL (CBL)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
CBL (CBL)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70/CBL/ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70-CBL)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70/CBL/ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70-CBL)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database CXCR4-mediated signaling events:
G beta/gamma complex (GNB1-GNG2)
→
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
(modification, activates)
Kumar et al., Immunity 2006, Patrussi et al., Blood 2007
Evidence: mutant phenotype, assay
-
NCI Pathway Database CXCR4-mediated signaling events:
Gi family/GTP/RGS1 complex (GNAI2_GNAI3_GNAO1_GNAO1_GNAZ_GNAI1-RGS1)
→
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
(modification, inhibits)
Kumar et al., Immunity 2006, Patrussi et al., Blood 2007
Evidence: mutant phenotype, assay
-
NCI Pathway Database CXCR4-mediated signaling events:
LCK (LCK)
→
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
(modification, collaborate)
Kumar et al., Immunity 2006, Patrussi et al., Blood 2007
Evidence: mutant phenotype, assay
-
NCI Pathway Database CXCR4-mediated signaling events:
LCK (LCK)
→
TCR/CD3/MHC II/CD4 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
Kumar et al., Immunity 2006, Patrussi et al., Blood 2007
Evidence: mutant phenotype, assay
-
NCI Pathway Database CXCR4-mediated signaling events:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
TCR/CD3/MHC II/CD4 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
Kumar et al., Immunity 2006, Patrussi et al., Blood 2007
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
CBL (CBL)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
SLAP-2 (SLA2)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
CBL (CBL)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
SLAP-2 (SLA2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
LAT/GRAP2/SLP76 complex (LAT-GRAP2-LCP2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Liu et al., J Immunol 2000
Evidence: mutant phenotype, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
HPK1 (MAP4K1)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Liu et al., J Immunol 2000
Evidence: mutant phenotype, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
LAT/GRAP2/SLP76/VAV1 complex (LAT-GRAP2-LCP2-VAV1)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, collaborate)
Reynolds et al., J Exp Med 2002, Fukazawa et al., J Biol Chem 1995, Carrera et al., J Biol Chem 1994, Exley et al., J Biol Chem 1994, de Aós et al., J Biol Chem 1997, Zhang et al., Cell 1998
Evidence: mutant phenotype, assay, physical interaction, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/3BP2 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-SH3BP2)
→
3BP2 (SH3BP2)
(modification, collaborate)
Deckert et al., Immunity 1998
Evidence: physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/3BP2 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-SH3BP2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Deckert et al., Immunity 1998
Evidence: physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
3BP2 (SH3BP2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Deckert et al., Immunity 1998
Evidence: physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
CSK/PAG1 complex (CSK-PAG1)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Brdicka et al., J Exp Med 2000
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
SHP1 (PTPN6)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70/3BP2 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-SH3BP2)
(modification, activates)
Yu et al., FEBS J 2006
Evidence: mutant phenotype
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
LCK (LCK)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Kersh et al., Science 1998, Tuosto et al., Eur J Immunol 1998, Viola et al., Science 1999
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
TCR/CD3/MHC II/CD4 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Kersh et al., Science 1998, Tuosto et al., Eur J Immunol 1998, Viola et al., Science 1999
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
LCK (LCK)
→
TCR/CD3/MHC II/CD4 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4)
(modification, collaborate)
Housden et al., Eur J Biochem 2003, Barber et al., Proc Natl Acad Sci U S A 1989, van Oers et al., J Exp Med 1996, Kersh et al., Science 1998, Tuosto et al., Eur J Immunol 1998, Viola et al., Science 1999
Evidence: mutant phenotype, assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/3BP2 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-SH3BP2)
→
None
(modification, activates)
Deckert et al., Immunity 1998
Evidence: reporter gene
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
ITK (ITK)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, activates)
Paz et al., Biochem J 2001, Perez-Villar et al., Biochemistry 2002, Zhang et al., Cell 1998
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
ZAP-70 (ZAP70)
(modification, collaborate)
Iwashima et al., Science 1994
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, collaborate)
Iwashima et al., Science 1994
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
B7 family/CD28 complex (CD28-CD80_CD86)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, activates)
Iwashima et al., Science 1994
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
ZAP-70 (ZAP70)
→
TCR/CD3/MHC I/CD8/LCK complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK)
(modification, collaborate)
Iwashima et al., Science 1994
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
LAT/GRAP2/SLP76/VAV1/PI3K Class IA complex (LAT-GRAP2-LCP2-VAV1)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
(modification, activates)
Villalba et al., J Cell Biol 2002, Reynolds et al., J Biol Chem 2004
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
PKC theta (PRKCQ)
(modification, activates)
Villalba et al., J Cell Biol 2002, Reynolds et al., J Biol Chem 2004
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
(modification, activates)
Villalba et al., J Cell Biol 2002, Reynolds et al., J Biol Chem 2004
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
B7 family/CD28 complex (CD28-CD80_CD86)
(modification, activates)
Raab et al., Proc Natl Acad Sci U S A 1995
Evidence: mutant phenotype, assay
-
Reactome Reaction:
CD3E
→
LCK
(indirect_complex)
Leo et al., J Clin Invest 2002, Huang et al., J Biol Chem 2004, Boggon et al., Oncogene 2004, Palacios et al., Oncogene 2004, Wilkinson et al., Expert reviews in molecular medicine 2005
-
Reactome Reaction:
CD3E
→
LCK
(reaction)
Carter et al., Immunol Res 2003, Huang et al., J Biol Chem 2004, Sheppard et al., FEBS Lett 2004, Palacios et al., Oncogene 2004, Wilkinson et al., Expert reviews in molecular medicine 2005, Keir et al., Annu Rev Immunol 2008, Fife et al., Immunol Rev 2008, van Oers et al., Semin Immunol 1995, Chu et al., Immunol Rev 1998
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
CD3E
—
LCK
(physical association, affinity chromatography technology)
Muñoz et al., J Biol Chem 2003*
-
IRef Hprd Interaction:
CD3E
—
LCK
(in vitro)
Guirado et al., Biochem Biophys Res Commun 2002*
-
IRef Hprd Interaction:
CD3E
—
LCK
(in vivo)
Guirado et al., Biochem Biophys Res Commun 2002*
-
IRef Intact Interaction:
Complex of VCP-ZAP70-VAV1-LCK-CD3E-PTPN22
(association, pull down)
Wu et al., J Biol Chem 2006
-
IRef Intact Interaction:
CD3E
—
LCK
(association, pull down)
Guirado et al., Biochem Biophys Res Commun 2002*
-
IRef Intact Interaction:
Complex of CD3E-ZAP70-VAV1-LCK-CD247-PTPN22
(association, pull down)
Wu et al., J Biol Chem 2006
-
IRef Ophid Interaction:
CD3E
—
LCK
(aggregation, confirmational text mining)
Guirado et al., Biochem Biophys Res Commun 2002*
-
IRef Ophid Interaction:
CD3E
—
LCK
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Doucey et al., Eur J Immunol 2001
:
Additional cross linking further increases
p56lck kinase activity,
induces translocation of
TCR/CD3 and other signaling molecules to lipid rafts and intracellular calcium mobilization
Danielian et al., Eur J Immunol 1992
:
Both
T cell receptor (TcR)-CD3 complex and CD2
increase the tyrosine kinase activity of
p56lck ... Therefore, to test directly the
role of
TcR-CD3 in CD2 induced activation of
p56lck , we utilized mutant variants of the Jurkat cell line lacking in cell surface TcR-CD3 ... We found that cell surface expression of
TcR-CD3 is not
required for the activation of
p56lck via CD2 but is necessary for the appearance of the reduced-electrophoretic-mobility form of p56lck observed after CD2 triggering
Thome et al., J Exp Med 1995
:
Evidence has suggested that the association of CD4 with
TCR/CD3/zeta requires the interaction of the protein tyrosine kinase
p56lck with CD4
Wang et al., J Immunol 1995
(Lymphoma, B-Cell) :
In vitro kinase assay studies also demonstrated that
TCR-CD3 engagement
increased the kinase activity of
Lck in normal T cells but not in T-TIL