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AKT3 — PDK1
Pathways - manually collected, often from reviews:
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
STRING interaction:
AKT3
—
PDK1
(interaction, mapped from kegg_pathways)
-
STRING interaction:
PDK1
—
AKT3
(interaction, mapped from kegg_pathways)
Text-mined interactions from Literome
Kim et al., J Biol Chem 2001
(MAP Kinase Signaling System) :
Cyclic AMP
inhibits Akt activity by blocking the membrane localization of
PDK1
Matsumoto et al., J Biol Chem 2001
:
Overexpression of
PDK1 with the use of an adenovirus vector
induced the phosphorylation and activation of
Akt ; epsilonKD inhibited, whereas wild-type PKCepsilon had no effect on, these actions of PDK1
Wen et al., Cell Signal 2003
:
The increased
Akt phosphorylation was
blocked by phosphatidylinositol 3-kinase inhibitor wortmannin and the newly identified PIP ( 3 ) -dependent kinases (
PDK ) inhibitor SB 203580
Hresko et al., J Biol Chem 2003
:
Immunodepleting cytosolic
PDK1 from an in vitro reaction containing plasma membrane and cytosol markedly
inhibited insulin stimulated phosphorylation of
Akt at the PDK1 site ( Thr-308 ) but had no effect on phosphorylation at the PDK2 site ( Ser-473 )
Lu et al., J Biomol Screen 2004
:
Activation of
Akt by
PDK1 is measured by quantitating the phosphorylation of Akt-specific substrate peptide using the IMAP assay format
Thimmaiah et al., J Biol Chem 2005
:
Akt inhibitory phenoxazines did not inhibit the activity of recombinant phosphatidylinositol 3'-kinase,
PDK1 , or SGK1 but potently
inhibited the kinase activity of recombinant
Akt and Akt deltaPH, a mutant lacking the pleckstrin homology domain ... Akt inhibitory phenoxazines did not inhibit the activity of recombinant phosphatidylinositol 3'-kinase,
PDK1 , or SGK1 but potently
inhibited the kinase activity of recombinant
Akt and Akt deltaPH, a mutant lacking the pleckstrin homology domain
Choi et al., Mol Cells 2005
(MAP Kinase Signaling System) :
PDK-1 activates
PI3-kinase/Akt signaling and regulates fundamental cellular functions, such as growth and survival
Dey et al., Cell Physiol Biochem 2005
:
PDK 1 dependent phosphorylation of PKCzeta and
Akt/PKB were also inhibited by palmitate
Li et al., J Gastrointest Surg 2006
(Pancreatic Neoplasms) :
Previous studies have demonstrated that OSU-03012, a celecoxib derivative, specifically inhibits
PDK-1 mediated phosphorylation of
Akt with IC ( 50 ) values in the low muM range
Tomita et al., Cancer Sci 2006
:
Phosphorylated
PDK1 is an
activator of
Akt by phosphorylating Akt ... Curcumin reduced phosphorylation of
PDK1 and
inhibited constitutive activation of
Akt
Zhang et al., Biochem Pharmacol 2007
(Prostatic Neoplasms) :
In PC-3 cells, CMEP has been found to inhibit only
AKT activation at both normal and serum-starvation conditions, not to
inhibit PI3K,
PDK1 , or MAPK ... In PC-3 cells, CMEP has been found to inhibit only
AKT activation at both normal and serum-starvation conditions, not to
inhibit PI3K,
PDK1 , or MAPK
Heo et al., Neurosci Lett 2009
:
Furthermore, coexpression of p62 and wild-type
PDK1 , the upstream kinase of Akt, further
increased Akt phosphorylation and cell viability, whereas the expression of kinase-defective PDK1 reversed the cytoprotective effects of p62 under oxidative stress
Lee et al., Mol Biol Cell 2009
(Alzheimer Disease) :
In vitro experiments identified that beta-amyloid ( Abeta ), especially oligomer preparations, specifically interrupted the
PDK dependent activation of
Akt
Lodeiro et al., PloS one 2009
:
This beta-arrestin scaffolded complex leads to full
activation of
Akt through
PDK1 and mTORC2, which are not associated to the complex
Rubio et al., J Mol Cell Cardiol 2009
(Myocardial Infarction) :
In comparison, overexpression of nuclear targeted
Akt does not
mediate increased translocation of either PI3K or
PDK1 indicating that accumulation of Akt does not drive PI3K or PDK1 into the nuclear compartment
Maurer et al., Cancer Res 2009
(Breast Neoplasms...) :
PIP ( 3 ) recruits PDK1 and AKT to the cell membrane through interactions with their pleckstrin homology domains, allowing
PDK1 to
activate AKT by phosphorylating it at residue threonine-308
Falasca et al., Br J Cancer 2010
(Adenocarcinoma...) :
2-O-Bn-InsP ( 5 ) specifically inhibits 3-phosphoinositide dependent protein kinase 1 ( PDK1 ) in vitro ( IC ( 50 ) in the low nanomolar range ) and the
PDK1 dependent phosphorylation of
Akt in cell lines and excised tumours
Yang et al., Cell cycle (Georgetown, Tex.) 2010
(Neoplasms) :
Akt activity is well-known
regulated through its phosphorylation at T308 and S473 by
PDK1 and mTOrC2, respectively
Liao et al., J Cell Sci 2010
:
Chemotactic activation of Dictyostelium AGC-family kinases
AKT and PKBR1
requires separate but coordinated functions of
PDK1 and TORC2 ... We show that activation of
AKT and PKBR1
requires PDK1-site phosphorylation, but that phosphorylation by TORC2 is insufficient for AKT or PKBR1 activation
Yang et al., Eur J Pharmacol 2010
:
These results suggest that plumbagin activates NAD ( P ) H oxidase, Src, and PI3K, and that the activated PI3K or
PDK1 subsequently
stimulate Akt and Ras-Raf-MEK1/2-ERK1/2 in 3T3-L1 cells
Shen et al., J Biol Chem 2010
:
PDK1 recruitment to the SHPS-1 signaling complex
enhances insulin-like growth factor-i stimulated
AKT activation and vascular smooth muscle cell survival
Reyes-Gordillo et al., Am J Pathol 2011
(Liver Cirrhosis) :
The lack of
AKT phosphorylation was not
due to the inhibition of PDGF-ßr phosphorylation, the activation of phosphoinositide 3-kinase (PI3K),
pyruvate dehydrogenase kinase isozyme 1 ( PDK1 ), and mammalian target of rapamycin (mTOR)
Fang et al., J Biol Chem 2012
(Prostatic Neoplasms) :
Significantly, androgen increased TORC2 mediated AKT S473 phosphorylation without affecting the
PDK1 mediated
AKT T308 phosphorylation or TORC1 activity
Keledjian et al., International journal of clinical and experimental medicine 2012
:
The current study determined if polyamines
regulate Akt activity by altering
PDK1
Ju et al., Cell Signal 2013
:
Syndecan 4 regulation of
PDK1 dependent
Akt activation
Najafov et al., Biochem J 2012
(Neoplasms) :
Consistent with this, we find combing
PDK1 and mTOR inhibitors
reduced Akt activation to below basal levels and markedly inhibited proliferation of all of the cell lines tested
Kang et al., J Immunol 2013
:
PTD-PDK1-Thr ( 513 ) -Ile
blocked binding between PDK1 and PKC?, phosphorylation of PKC? Thr ( 538 ), and activation of both NF-?B and
AKT
Li et al., Nan Fang Yi Ke Da Xue Xue Bao 2013
:
To study the effect of homcysteine on the expression of 3-phosphoinositide dependent protein kinase 1 ( PDK1 ) in the adipose tissue and explore whether
PDK1 inhibits
p-Akt ( Thr-308 ) expression and affect PI3K/Akt signal pathway to decrease glucose uptake and utilization
Walker et al., Biochem J 1998
:
The
activation of PKBbeta and
PKBgamma by
PDK1 was accompanied by the phosphorylation of the residues equivalent to Thr308 in PKBalpha, namely Thr309 ( PKBbeta ) and Thr305 ( PKBgamma ) ...
PKBgamma which had been
activated by
PDK1 possessed a substrate specificity identical with that of PKBalpha and PKBbeta towards a range of peptides