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RAF1 — YWHAB

Pathways - manually collected, often from reviews:

• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: KSR/14-3-3 /MEK1-2 complex (KSR1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB-MAP2K1_MAP2K2) → RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF) (modification, collaborate)
  Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Rajakulendran et al., Nature 2009, Ritt et al., Mol Cell Biol 2010, Reuter et al., J Biol Chem 1995
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: Erk1-2-active (MAPK3/MAPK1) → RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, activates)
  Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: Erk1-2-active (MAPK3/MAPK1) → RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, activates)
  Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → RAF1/14-3-3 (dimer) complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → 14-3-3 family (YWHAH/YWHAZ/YWHAQ/SFN/YWHAE/YWHAG/YWHAB) (modification, collaborate)
  Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: PP2A complex (PPP2R1A-PPP2R2B-PPP2CA) → RAF1/14-3-3 (dimer) complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, activates)
  Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 (dimer) complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → 14-3-3 family (YWHAH/YWHAZ/YWHAQ/SFN/YWHAE/YWHAG/YWHAB) (modification, collaborate)
  Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 (dimer) complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → KSR (KSR1) (modification, collaborate) Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: RAF1/14-3-3 (dimer) complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) → RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS) (modification, collaborate)
  Mason et al., EMBO J 1999, Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Dhillon et al., Cell Signal 2009, Ritt et al., Mol Cell Biol 2010, Michaud et al., Mol Cell Biol 1995, Muslin et al., Cell 1996
  Evidence: assay, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: KSR/14-3-3 /MEK1-2 complex (KSR1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB-MAP2K1_MAP2K2) → RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB) (modification, collaborate)
  McKay et al., Proc Natl Acad Sci U S A 2009
  Evidence: mutant phenotype, assay
• Reactome Reaction: YWHAB → RAF1 (indirect_complex) Morrison et al., Curr Opin Cell Biol 1997*
• Reactome Reaction: YWHAB → RAF1 (reaction) Morrison et al., Curr Opin Cell Biol 1997*
• Reactome Reaction: YWHAB → RAF1 (direct_complex) Morrison et al., Curr Opin Cell Biol 1997*

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: RAF1 — YWHAB Yuryev et al., Genomics 2003
• IRef Bind_translation Interaction: RAF1 — YWHAB (two hybrid) Yuryev et al., Genomics 2003
• IRef Biogrid Interaction: RAF1 — YWHAB (direct interaction, two hybrid) Vincenz et al., J Biol Chem 1996
• IRef Biogrid Interaction: RAF1 — YWHAB (direct interaction, two hybrid) Conklin et al., Proc Natl Acad Sci U S A 1995*
• IRef Biogrid Interaction: RAF1 — YWHAB (physical association, affinity chromatography technology) Subramanian et al., Exp Cell Res 2001
• IRef Biogrid Interaction: RAF1 — YWHAB (physical association, affinity chromatography technology) Rajalingam et al., Nat Cell Biol 2005
• IRef Biogrid Interaction: RAF1 — YWHAB (physical association, affinity chromatography technology) Zhu et al., Mol Biol Cell 2005
• IRef Biogrid Interaction: RAF1 — YWHAB (direct interaction, two hybrid) Yuryev et al., Genomics 2003
• IRef Biogrid Interaction: RAF1 — YWHAB (direct interaction, pull down) Truong et al., Proteins 2002*
• IRef Biogrid Interaction: RAF1 — YWHAB (physical association, affinity chromatography technology) Van Der Hoeven et al., Biochem J 2000
• IRef Biogrid Interaction: RAF1 — YWHAB (direct interaction, two hybrid) Yuryev et al., Mol Cell Biol 2000*
• MIPS CORUM BRAF-RAF1-14-3-3 complex: BRAF-RAF1-14-3-3 complex complex (BRAF-RAF1-SFN-YWHAB-YWHAE-YWHAG-YWHAH-YWHAQ-YWHAZ) Chen et al., J Biol Chem 2008
• IRef Corum Interaction: Complex of RAF1-BRAF-YWHAQ-YWHAB-SFN-YWHAG-YWHAE-YWHAZ-YWHAH (association, anti tag coimmunoprecipitation) Chen et al., J Biol Chem 2008
• IRef Hprd Interaction: RAF1 — YWHAB (in vivo) Conklin et al., Proc Natl Acad Sci U S A 1995*, Vincenz et al., J Biol Chem 1996
• IRef Hprd Interaction: RAF1 — YWHAB (in vitro) Conklin et al., Proc Natl Acad Sci U S A 1995*, Vincenz et al., J Biol Chem 1996
• IRef Intact Interaction: RAF1 — YWHAB (association, biochemical) Subramanian et al., Exp Cell Res 2001
• IRef Intact Interaction: RAF1 — YWHAB (physical association, two hybrid) Yuryev et al., Genomics 2003
• IRef Intact Interaction: RAF1 — YWHAB (association, biochemical) Dhillon et al., Cell Signal 2009
• IRef Intact Interaction: RAF1 — YWHAB (physical association, two hybrid) Conklin et al., Proc Natl Acad Sci U S A 1995*
• IRef Intact Interaction: RAF1 — YWHAB (physical association, anti bait coimmunoprecipitation) Conklin et al., Proc Natl Acad Sci U S A 1995*
• IRef Intact Interaction: RAF1 — YWHAB (physical association, two hybrid) Li et al., EMBO J 1995*
• IRef Intact Interaction: RAF1 — YWHAB (physical association, two hybrid) Fantl et al., Nature 1994*
• IRef Intact Interaction: RAF1 — YWHAB (physical association, two hybrid) Freed et al., Science 1994*
• IRef Intact Interaction: Complex of 222 proteins (association, tandem affinity purification) Koch et al., Cell cycle (Georgetown, Tex.) 2007
• IRef Intact Interaction: Complex of 143 proteins (association, anti tag coimmunoprecipitation)
• IRef Intact Interaction: Complex of 78 proteins (association, tandem affinity purification) Gloeckner et al., Proteomics 2007
• IRef Intact Interaction: Complex of 26 proteins (association, anti tag coimmunoprecipitation)
• IRef Intact Interaction: Complex of 11 proteins (association, tandem affinity purification) Gloeckner et al., Proteomics 2007
• IRef Intact Interaction: Complex of CDC25A-RAF1-YWHAB-YWHAB-CDC25A-RAF1-RAF1-YWHAB-CDC25A (physical association, anti bait coimmunoprecipitation) Conklin et al., Proc Natl Acad Sci U S A 1995*
• IRef Ophid Interaction: RAF1 — YWHAB (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: RAF1 — YWHAB (aggregation, confirmational text mining) Truong et al., Proteins 2002*

Text-mined interactions from Literome

Banihashemi et al., Mol Endocrinol 2002 : TRH induced c-Raf but not B-Raf activation, and the D2S receptor inhibited both TRH induced c-Raf and basal B-Raf kinase activity
Röring et al., EMBO J 2012 (MAP Kinase Signaling System) : In contrast, B-Raf ( wt ) and Raf-1 ( wt ) mediated signalling triggered by oncogenic Ras as well as the paradoxical activation of Raf-1 by kinase inactivated B-Raf require an intact DIF. Surprisingly, the B-Raf DIF is not required for dimerisation between Raf-1 and B-Raf, which was inactivated by the D594A mutation, sorafenib or PLX4720
Liaw et al., Proc Natl Acad Sci U S A 1993 : Activated Raf-1 then leads , by an undefined mechanism, to the transcriptional activation of the tailless (tll) gene ; the tll gene product, itself a transcription factor, subsequently regulates the expression of an array of target genes
Tombes et al., Biochem J 1998 : In contrast to hepatocytes, NGF treatment of PC12 pheochromocytoma cells caused large sustained activations of B-Raf and p42 ( MAPkinase ), and a lower phasic activation of Raf-1