Description: Homo sapiens ribophorin II (RPN2), transcript variant 1, mRNA. RefSeq Summary (NM_002951): This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]. Transcript (Including UTRs) Position: hg19 chr20:35,807,456-35,870,025 Size: 62,570 Total Exon Count: 17 Strand: + Coding Region Position: hg19 chr20:35,807,760-35,869,718 Size: 61,959 Coding Exon Count: 17
ID:RPN2_HUMAN DESCRIPTION: RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; EC=2.4.1.119; AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; AltName: Full=RIBIIR; AltName: Full=Ribophorin II; Short=RPN-II; AltName: Full=Ribophorin-2; Flags: Precursor; FUNCTION: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + protein L- asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L- asparagine. SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). TISSUE SPECIFICITY: Expressed in all tissues tested. SIMILARITY: Belongs to the SWP1 family.
To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3
Genetic Association Studies of Complex Diseases and Disorders
Genetic Association Database (archive): RPN2 CDC HuGE Published Literature: RPN2 Positive Disease Associations: hippocampal atrophy
, Hippocampus Related Studies:
hippocampal atrophy Potkin ,et al. 2009, Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease, PloS one 2010 4- 8 : e6501.
[PubMed 19668339]
Hippocampus Steven G Potkin et al. PloS one 2010, Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease., PloS one.
[PubMed 19668339]
Using hippocampal atrophy as a quantitative phenotype in a genome-wide scan, we have identified candidate risk genes for sporadic Alzheimers disease that merit further investigation.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF05817 - Oligosaccharyltransferase subunit Ribophorin II
ModBase Predicted Comparative 3D Structure on P04844
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006464 cellular protein modification process GO:0006486 protein glycosylation GO:0006487 protein N-linked glycosylation GO:0007568 aging GO:0018279 protein N-linked glycosylation via asparagine GO:0042493 response to drug
Y00282 - Human mRNA for ribophorin II. AK096243 - Homo sapiens cDNA FLJ38924 fis, clone NT2NE2011896, highly similar to Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit precursor (EC 2.4.1.119). BC020222 - Homo sapiens ribophorin II, mRNA (cDNA clone MGC:31916 IMAGE:3640766), complete cds. JD408624 - Sequence 389648 from Patent EP1572962. AK315801 - Homo sapiens cDNA, FLJ96923, highly similar to Homo sapiens ribophorin II (RPN2), mRNA. BC003560 - Homo sapiens ribophorin II, mRNA (cDNA clone MGC:1817 IMAGE:3546673), complete cds. JD395120 - Sequence 376144 from Patent EP1572962. JD407382 - Sequence 388406 from Patent EP1572962. JD400196 - Sequence 381220 from Patent EP1572962. AK308683 - Homo sapiens cDNA, FLJ98724. CR456899 - Homo sapiens full open reading frame cDNA clone RZPDo834H0314D for gene RPN2, ribophorin II; complete cds, incl. stopcodon. DQ895552 - Synthetic construct Homo sapiens clone IMAGE:100010012; FLH187269.01L; RZPDo839F0762D ribophorin II (RPN2) gene, encodes complete protein. DQ892347 - Synthetic construct clone IMAGE:100004977; FLH187273.01X; RZPDo839F0772D ribophorin II (RPN2) gene, encodes complete protein. CU678504 - Synthetic construct Homo sapiens gateway clone IMAGE:100020343 5' read RPN2 mRNA. KJ897495 - Synthetic construct Homo sapiens clone ccsbBroadEn_06889 RPN2 gene, encodes complete protein. AK296126 - Homo sapiens cDNA FLJ60278 complete cds, highly similar to Dolichyl-diphosphooligosaccharide--proteinglycosyltransferase 63 kDa subunit precursor (EC 2.4.1.119). BC002380 - Homo sapiens ribophorin II, mRNA (cDNA clone IMAGE:2961244), partial cds. DJ434623 - use of RPN2 gene expression inhibitor. DJ434598 - use of RPN2 gene expression inhibitor. BC013028 - Homo sapiens ribophorin II, mRNA (cDNA clone IMAGE:3532152), partial cds. AK123690 - Homo sapiens cDNA FLJ41696 fis, clone HCHON2001853. JD113202 - Sequence 94226 from Patent EP1572962. JD554015 - Sequence 535039 from Patent EP1572962. JD044525 - Sequence 25549 from Patent EP1572962. JD050495 - Sequence 31519 from Patent EP1572962. JD467194 - Sequence 448218 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00510 - N-Glycan biosynthesis hsa01100 - Metabolic pathways
Reactome (by CSHL, EBI, and GO)
Protein P04844 (Reactome details) participates in the following event(s):
R-HSA-1799326 Signal-containing nascent peptide translocates to endoplasmic reticulum R-HSA-1799329 Signal peptidase hydrolyzes signal peptide from ribosome-associated nascent protein R-HSA-446209 Transfer of N-glycan to the protein R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane R-HSA-72766 Translation R-HSA-446203 Asparagine N-linked glycosylation R-HSA-392499 Metabolism of proteins R-HSA-597592 Post-translational protein modification