Gene interactions and pathways from curated databases and text-mining
J Biol Chem 1999, PMID: 10400692

Requirement for Akt (protein kinase B) in insulin-induced activation of glycogen synthase and phosphorylation of 4E-BP1 (PHAS-1).

Takata, M; Ogawa, W; Kitamura, T; Hino, Y; Kuroda, S; Kotani, K; Klip, A; Gingras, A C; Sonenberg, N; Kasuga, M

The roles of Akt (protein kinase B) and the atypical lambda isoform of protein kinase C (PKClambda), both of which act downstream of phosphoinositide 3-kinase, in the activation of glycogen synthase and phosphorylation of 4E-BP1 (PHAS-1) in response to insulin were investigated. A mutant Akt (Akt-AA) in which the phosphorylation sites targeted by growth factors are replaced by alanine was shown to inhibit insulin-induced activation of both Akt and glycogen synthase in L6 myotubes. Expression of a mutant Akt in which Lys179 in the kinase domain was replaced by aspartate also inhibited insulin-induced activation of glycogen synthase but had no effect on insulin activation of endogenous Akt. A kinase-defective mutant of PKClambda (lambdaDeltaNKD), which prevents insulin-induced activation of PKClambda, did not affect the activation of glycogen synthase by insulin. Insulin-induced phosphorylation of 4E-BP1 was inhibited by Akt-AA in Chinese hamster ovary cells. However, lambdaDeltaNKD had no effect on 4E-BP1 phosphorylation induced by insulin. These data suggest that Akt, but not PKClambda, is required for insulin activation of glycogen synthase and for insulin-induced phosphorylation of 4E-BP1.

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Text Mining Data

glycogen synthase → Akt: " Requirement for Akt ( protein kinase B ) in insulin induced activation of glycogen synthase and phosphorylation of 4E-BP1 ( PHAS-1 ) "

glycogen synthase → insulin: " Requirement for Akt ( protein kinase B ) in insulin induced activation of glycogen synthase and phosphorylation of 4E-BP1 ( PHAS-1 ) "

glycogen synthase ⊣ Akt: " The roles of Akt ( protein kinase B ) and the atypical lambda isoform of protein kinase C ( PKClambda ), both of which act downstream of phosphoinositide 3-kinase, in the activation of glycogen synthase and phosphorylation of 4E-BP1 ( PHAS-1 ) in response to insulin were investigated "

glycogen synthase → insulin: " The roles of Akt ( protein kinase B ) and the atypical lambda isoform of protein kinase C ( PKClambda ), both of which act downstream of phosphoinositide 3-kinase, in the activation of glycogen synthase and phosphorylation of 4E-BP1 ( PHAS-1 ) in response to insulin were investigated "

glycogen synthase ⊣ Akt: " A mutant Akt ( Akt-AA ) in which the phosphorylation sites targeted by growth factors are replaced by alanine was shown to inhibit insulin induced activation of both Akt and glycogen synthase in L6 myotubes "

Akt → insulin: " A mutant Akt ( Akt-AA ) in which the phosphorylation sites targeted by growth factors are replaced by alanine was shown to inhibit insulin induced activation of both Akt and glycogen synthase in L6 myotubes "

glycogen synthase → insulin: " A mutant Akt ( Akt-AA ) in which the phosphorylation sites targeted by growth factors are replaced by alanine was shown to inhibit insulin induced activation of both Akt and glycogen synthase in L6 myotubes "

glycogen synthase ⊣ Akt: " Expression of a mutant Akt in which Lys179 in the kinase domain was replaced by aspartate also inhibited insulin induced activation of glycogen synthase but had no effect on insulin activation of endogenous Akt "

glycogen synthase → insulin: " Expression of a mutant Akt in which Lys179 in the kinase domain was replaced by aspartate also inhibited insulin induced activation of glycogen synthase but had no effect on insulin activation of endogenous Akt "

insulin → Akt: " Expression of a mutant Akt in which Lys179 in the kinase domain was replaced by aspartate also inhibited insulin induced activation of glycogen synthase but had no effect on insulin activation of endogenous Akt "

glycogen synthase → insulin: " A kinase-defective mutant of PKClambda ( lambdaDeltaNKD ), which prevents insulin induced activation of PKClambda, did not affect the activation of glycogen synthase by insulin "

4E-BP1 ⊣ Akt-AA: " Insulin induced phosphorylation of 4E-BP1 was inhibited by Akt-AA in Chinese hamster ovary cells "

4E-BP1 → Insulin: " Insulin induced phosphorylation of 4E-BP1 was inhibited by Akt-AA in Chinese hamster ovary cells "

4E-BP1 → insulin: " However, lambdaDeltaNKD had no effect on 4E-BP1 phosphorylation induced by insulin "

4E-BP1 → Akt: " These data suggest that Akt , but not PKClambda, is required for insulin activation of glycogen synthase and for insulin induced phosphorylation of 4E-BP1 "

4E-BP1 → insulin: " These data suggest that Akt, but not PKClambda, is required for insulin activation of glycogen synthase and for insulin induced phosphorylation of 4E-BP1 "

glycogen synthase → Akt: " These data suggest that Akt , but not PKClambda, is required for insulin activation of glycogen synthase and for insulin induced phosphorylation of 4E-BP1 "

insulin → Akt: " These data suggest that Akt , but not PKClambda, is required for insulin activation of glycogen synthase and for insulin induced phosphorylation of 4E-BP1 "

insulin → glycogen synthase: " These data suggest that Akt, but not PKClambda, is required for insulin activation of glycogen synthase and for insulin induced phosphorylation of 4E-BP1 "

Manually curated Databases

No curated data.