J Biol Chem 2005,
PMID: 16120607
Newcomb, Dawn C; Sajjan, Uma; Nanua, Suparna; Jia, Yue; Goldsmith, Adam M; Bentley, J Kelley; Hershenson, Marc B
Rhinovirus (RV) is a common cause of asthma exacerbations. The signaling mechanisms regulating RV-induced airway epithelial cell responses have not been well studied. We examined the role of phosphatidylinositol (PI) 3-kinase in RV-induced interleukin (IL)-8 expression. Infection of 16HBE14o- human bronchial epithelial cells with RV39 induced rapid activation of PI 3-kinase and phosphorylation of Akt, a downstream effector of PI 3-kinase. RV39 also colocalized with cit-Akt-PH, a citrogen-tagged fluorescent fusion protein encoding the pleckstrin homology domain of Akt, indicating that 3-phosphorylated PI accumulates at the site of RV infection. Inhibition of PI 3-kinase and Akt attenuated RV39-induced NF-kappaB transactivation and IL-8 expression. Inhibition of PI 3-kinase also blocked internalization of labeled RV39 into 16HBE14o- cells, suggesting that the requirement of PI 3-kinase for RV39-induced IL-8 expression, at least in part, relates to its role in viral endocytosis.
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Text Mining Data
interleukin-8 → Phosphatidylinositol 3-kinase: "
Phosphatidylinositol 3-kinase is
required for rhinovirus induced airway epithelial cell
interleukin-8 expression
"
interleukin (IL)-8 ⊣ phosphatidylinositol (PI) 3-kinase: "
We examined the role of phosphatidylinositol (PI) 3-kinase in RV-induced interleukin (IL)-8 expression
"
IL-8 → Akt: "
Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression
"
IL-8 → PI 3-kinase: "
Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression
"
NF-kappaB → Akt: "
Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression
"
NF-kappaB → PI 3-kinase: "
Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression
"
Manually curated Databases
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