Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2005, PMID: 16120607

Phosphatidylinositol 3-kinase is required for rhinovirus-induced airway epithelial cell interleukin-8 expression.

Newcomb, Dawn C; Sajjan, Uma; Nanua, Suparna; Jia, Yue; Goldsmith, Adam M; Bentley, J Kelley; Hershenson, Marc B

Rhinovirus (RV) is a common cause of asthma exacerbations. The signaling mechanisms regulating RV-induced airway epithelial cell responses have not been well studied. We examined the role of phosphatidylinositol (PI) 3-kinase in RV-induced interleukin (IL)-8 expression. Infection of 16HBE14o- human bronchial epithelial cells with RV39 induced rapid activation of PI 3-kinase and phosphorylation of Akt, a downstream effector of PI 3-kinase. RV39 also colocalized with cit-Akt-PH, a citrogen-tagged fluorescent fusion protein encoding the pleckstrin homology domain of Akt, indicating that 3-phosphorylated PI accumulates at the site of RV infection. Inhibition of PI 3-kinase and Akt attenuated RV39-induced NF-kappaB transactivation and IL-8 expression. Inhibition of PI 3-kinase also blocked internalization of labeled RV39 into 16HBE14o- cells, suggesting that the requirement of PI 3-kinase for RV39-induced IL-8 expression, at least in part, relates to its role in viral endocytosis.

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Text Mining Data

interleukin-8 → Phosphatidylinositol 3-kinase: " Phosphatidylinositol 3-kinase is required for rhinovirus induced airway epithelial cell interleukin-8 expression "

interleukin (IL)-8 ⊣ phosphatidylinositol (PI) 3-kinase: " We examined the role of phosphatidylinositol (PI) 3-kinase in RV-induced interleukin (IL)-8 expression "

IL-8 → Akt: " Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression "

IL-8 → PI 3-kinase: " Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression "

NF-kappaB → Akt: " Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression "

NF-kappaB → PI 3-kinase: " Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression "

Manually curated Databases

No curated data.