Gene interactions and pathways from curated databases and text-mining
Nat Cell Biol 2006, PMID: 16622419

The human CENP-A centromeric nucleosome-associated complex.

Foltz, Daniel R; Jansen, Lars E T; Black, Ben E; Bailey, Aaron O; Yates, John R; Cleveland, Don W

The basic element for chromosome inheritance, the centromere, is epigenetically determined in mammals. The prime candidate for specifying centromere identity is the array of nucleosomes assembled with CENP-A, the centromere-specific histone H3 variant. Here, we show that CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENP-M, CENP-N and CENP-T), along with CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Facilitates chromatin transcription (FACT) and nucleophosmin-1 (previously implicated in transcriptional chromatin remodelling and as a multifunctional nuclear chaperone, respectively) are absent from histone H3-containing nucleosomes, but are stably recruited to CENP-A nucleosomes independent of CENP-A NAC. Seven new CENP-A-nucleosome distal (CAD) centromere components (CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S) are identified as assembling on the CENP-A NAC. The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: CENPA — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPA — H2AFY (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPA — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H4A — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H2AFX — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPH — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I — CBX5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I — H2AFY (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPN — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPO (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPQ (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPO — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPH (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST2H2AC — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPN — CENPL (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPQ — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H2AFX — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUPT16H — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPN — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H1A — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPP — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST3H2BB — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPC — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPN — CENPQ (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H2AFZ — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPP — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPI — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H1A — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPK — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSRP1 — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPA — CENPC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPL — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST3H2BB — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPN — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPI — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPT — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPK — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPH — CENPN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPL (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPO — CENPM (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BANF1 — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPK — CENPU (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPU — CENPI (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST2H2AC — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPB — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHAF1A — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CBX1 — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H4A — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPA — HJURP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHAF1B — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I — RBBP7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I — CENPA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H2AFZ — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBBP4 — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CENPT — CENPA (physical association, affinity chromatography technology)
  • MIPS CORUM CENP-A NAC-CAD complex: CENP-A NAC-CAD complex complex (APITD1-CENPC-CENPH-CENPK-CENPL-CENPM-CENPN-CENPO-CENPP-CENPQ-CENPT-ITGB3BP-CENPU)
  • MIPS CORUM CENP-A nucleosome associated complex: CENP-A nucleosome associated complex complex (CENPC-CENPH-CENPM-CENPN-CENPT-CENPU)
  • IRef Corum Interaction: Complex of 14 proteins (association, tandem affinity purification)
  • IRef Corum Interaction: Complex of CENPC-CENPU-CENPT-CENPN-CENPH-CENPM (association, tandem affinity purification)
In total, 305 gene pairs are associated to this article in curated databases