Nat Cell Biol 2006,
PMID: 16622419
Foltz, Daniel R; Jansen, Lars E T; Black, Ben E; Bailey, Aaron O; Yates, John R; Cleveland, Don W
The basic element for chromosome inheritance, the centromere, is epigenetically determined in mammals. The prime candidate for specifying centromere identity is the array of nucleosomes assembled with CENP-A, the centromere-specific histone H3 variant. Here, we show that CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENP-M, CENP-N and CENP-T), along with CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Facilitates chromatin transcription (FACT) and nucleophosmin-1 (previously implicated in transcriptional chromatin remodelling and as a multifunctional nuclear chaperone, respectively) are absent from histone H3-containing nucleosomes, but are stably recruited to CENP-A nucleosomes independent of CENP-A NAC. Seven new CENP-A-nucleosome distal (CAD) centromere components (CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S) are identified as assembling on the CENP-A NAC. The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling.
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Manually curated Databases
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IRef Biogrid Interaction:
CENPA
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPA
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H2AFY
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPA
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NPM1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H4A
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
H2AFX
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPH
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
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CBX5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
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H2AFY
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IRef Biogrid Interaction:
CENPN
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPO
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPQ
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPO
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPH
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST2H2AC
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPN
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CENPL
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPQ
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
H2AFX
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SUPT16H
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPN
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H1A
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPP
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST3H2BB
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPC
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPN
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CENPQ
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
H2AFZ
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPP
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPI
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H1A
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPK
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SSRP1
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPA
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CENPC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPL
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST3H2BB
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPN
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPI
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPT
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPK
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPH
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CENPN
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPL
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPO
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CENPM
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
BANF1
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPK
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CENPU
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPU
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CENPI
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST2H2AC
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPB
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CHAF1A
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CBX1
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H4A
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPA
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HJURP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CHAF1B
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
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RBBP7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
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CENPA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
H2AFZ
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RBBP4
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
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HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CENPT
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CENPA
(physical association, affinity chromatography technology)
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MIPS CORUM CENP-A NAC-CAD complex:
CENP-A NAC-CAD complex complex (APITD1-CENPC-CENPH-CENPK-CENPL-CENPM-CENPN-CENPO-CENPP-CENPQ-CENPT-ITGB3BP-CENPU)
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MIPS CORUM CENP-A nucleosome associated complex:
CENP-A nucleosome associated complex complex (CENPC-CENPH-CENPM-CENPN-CENPT-CENPU)
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IRef Corum Interaction:
Complex of 14 proteins
(association, tandem affinity purification)
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IRef Corum Interaction:
Complex of CENPC-CENPU-CENPT-CENPN-CENPH-CENPM
(association, tandem affinity purification)
In total, 305 gene pairs are associated to this article in curated databases