Cancer Res 2010,
PMID: 20858719
Dafni, Hagit; Larson, Peder E Z; Hu, Simon; Yoshihara, Hikari A I; Ward, Christopher S; Venkatesh, Humsa S; Wang, Chunsheng; Zhang, Xiaoliang; Vigneron, Daniel B; Ronen, Sabrina M
The recent development of hyperpolarized (13)C magnetic resonance spectroscopic imaging provides a novel method for in vivo metabolic imaging with potential applications for detection of cancer and response to treatment. Chemotherapy-induced apoptosis was shown to decrease the flux of hyperpolarized (13)C label from pyruvate to lactate due to depletion of NADH, the coenzyme of lactate dehydrogenase. In contrast, we show here that in PC-3MM2 tumors, inhibition of platelet-derived growth factor receptor with imatinib reduces the conversion of hyperpolarized pyruvate to lactate by lowering the expression of lactate dehydrogenase itself. This was accompanied by reduced expression of vascular endothelial growth factor and glutaminase, and is likely mediated by reduced expression of their transcriptional factors hypoxia-inducible factor-1 and c-Myc. Our results indicate that hyperpolarized (13)C MRSI could potentially detect the molecular effect of various cell signaling inhibitors, thus providing a radiation-free method to predict tumor response.
Diseases/Pathways annotated by Medline MESH: Neovascularization, Pathologic, Prostatic Neoplasms
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Text Mining Data
vascular endothelial growth factor → c-Myc: "
This was accompanied by reduced expression of
vascular endothelial growth factor and glutaminase, and is likely
mediated by reduced expression of their transcriptional factors hypoxia-inducible factor-1 and
c-Myc
"
glutaminase → c-Myc: "
This was accompanied by reduced expression of vascular endothelial growth factor and glutaminase , and is likely mediated by reduced expression of their transcriptional factors hypoxia-inducible factor-1 and c-Myc
"
Manually curated Databases
No curated data.