J Toxicol Sci 2012,
Jung, Eun-Jeong; Lee, Kang-Yo; Lee, Byung-Hoon
Orotic acid (OA) is a tumor promoter of experimental liver carcinogenesis initiated by DNA reactive carcinogens, the molecular mechanisms of which have not been fully elucidated. OA increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1). The effects of OA on mTORC1 activation, cell proliferation, and cell-cycle progression to S and G2/M phases were completely reversed by rapamycin. Activation of AMPK by a constitutively active mutant or aminoimidazole carboxamide ribonucleotide (AICAR) rescued the effects of OA. In conclusion, OA increases the proliferation and decreases the starvation-induced apoptosis of SK-Hep1 cells via mTORC1 activation mediated by negative regulation of AMPK.
Document information provided by NCBI PubMed
Text Mining Data
mTORC1 → AMPK: " In conclusion, OA increases the proliferation and decreases the starvation induced apoptosis of SK-Hep1 cells via mTORC1
by negative regulation of AMPK
Manually curated Databases
No curated data.