Gene interactions and pathways from curated databases and text-mining
Proc Natl Acad Sci U S A 1997, PMID: 9326583

D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain.

Huang, L J; Durick, K; Weiner, J A; Chun, J; Taylor, S S

Subcellular localization directed by specific A kinase anchoring proteins (AKAPs) is a mechanism for compartmentalization of cAMP-dependent protein kinase (PKA). Using a two-hybrid screen, a novel AKAP was isolated. Because it interacts with both the type I and type II regulatory subunits, it was defined as a dual specific AKAP or D-AKAP1. Here we report the cloning and characterization of another novel cDNA isolated from that screen. This new member of the D-AKAP family, D-AKAP2, also binds both types of regulatory subunits. A message of 5 kb pairs was detected for D-AKAP2 in all embryonic stages and in all adult tissues tested. In brain, skeletal muscle, kidney, and testis, a 10-kb mRNA was identified. In testis, several small mRNAs were observed. Therefore, D-AKAP2 represents a novel family of proteins. cDNA cloning from a mouse testis library identified the full length D-AKAP2. It is composed of 372 amino acids which includes the R binding fragment, residues 333-372, at its C-terminus. Based on coprecipitation assays, the R binding domain interacts with the N-terminal dimerization domain of RIalpha and RIIalpha. A putative RGS domain was identified near the N-terminal region of D-AKAP2. The presence of this domain raises the intriguing possibility that D-AKAP2 may interact with a Galpha protein thus providing a link between the signaling machinery at the plasma membrane and the downstream kinase.

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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: PRKAR1A — AKAP10 (direct interaction, pull down)
  • IRef Biogrid Interaction: PRKAR1A — AKAP10 (direct interaction, two hybrid)
  • IRef Hprd Interaction: PRKAR1A — AKAP10 (in vitro)
  • IRef Hprd Interaction: PRKAR1A — AKAP10 (two hybrid)
  • IRef Hprd Interaction: PRKAR2B — AKAP10 (in vitro)
  • IRef Hprd Interaction: AKAP10 — PRKAR2A (in vitro)
  • IRef Ophid Interaction: PRKAR2B — AKAP10 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: PRKAR2A — AKAP10 (aggregation, confirmational text mining)
  • Reactome Reaction: AKAP10 → PRKAR1A (reaction)
  • Reactome Reaction: AKAP10 → PRKAR1B (reaction)
  • Reactome Reaction: AKAP1 → PRKAR1B (reaction)
  • Reactome Reaction: AKAP10 → PRKACG (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKACG (reaction)
  • Reactome Reaction: AKAP10 → PRKACB (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKACB (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKAR1B (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKACG (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKAR1A (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKACA (reaction)
  • Reactome Reaction: AKAP1 → PRKAR2B (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKAR2A (reaction)
  • Reactome Reaction: AKAP1 → PRKACB (reaction)
  • Reactome Reaction: AKAP10 → PRKAR2A (reaction)
  • Reactome Reaction: AKAP10 → PRKACA (indirect_complex)
  • Reactome Reaction: AKAP10 → PRKAR2B (reaction)
  • Reactome Reaction: AKAP10 → PRKAR1A (indirect_complex)
  • Reactome Reaction: AKAP10 → PRKACG (reaction)
  • Reactome Reaction: AKAP1 → PRKAR2A (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKAR1A (reaction)
  • Reactome Reaction: AKAP10 → PRKACB (reaction)
  • Reactome Reaction: AKAP10 → PRKAR2B (indirect_complex)
  • Reactome Reaction: AKAP10 → PRKACA (reaction)
  • Reactome Reaction: AKAP1 → PRKAR2B (reaction)
  • Reactome Reaction: AKAP10 → PRKAR1B (indirect_complex)
  • Reactome Reaction: AKAP1 → PRKACA (indirect_complex)
  • Reactome Reaction: AKAP10 → PRKAR2A (indirect_complex)
In total, 14 gene pairs are associated to this article in curated databases