Proc Natl Acad Sci U S A 1997,
PMID: 9326583
Huang, L J; Durick, K; Weiner, J A; Chun, J; Taylor, S S
Subcellular localization directed by specific A kinase anchoring proteins (AKAPs) is a mechanism for compartmentalization of cAMP-dependent protein kinase (PKA). Using a two-hybrid screen, a novel AKAP was isolated. Because it interacts with both the type I and type II regulatory subunits, it was defined as a dual specific AKAP or D-AKAP1. Here we report the cloning and characterization of another novel cDNA isolated from that screen. This new member of the D-AKAP family, D-AKAP2, also binds both types of regulatory subunits. A message of 5 kb pairs was detected for D-AKAP2 in all embryonic stages and in all adult tissues tested. In brain, skeletal muscle, kidney, and testis, a 10-kb mRNA was identified. In testis, several small mRNAs were observed. Therefore, D-AKAP2 represents a novel family of proteins. cDNA cloning from a mouse testis library identified the full length D-AKAP2. It is composed of 372 amino acids which includes the R binding fragment, residues 333-372, at its C-terminus. Based on coprecipitation assays, the R binding domain interacts with the N-terminal dimerization domain of RIalpha and RIIalpha. A putative RGS domain was identified near the N-terminal region of D-AKAP2. The presence of this domain raises the intriguing possibility that D-AKAP2 may interact with a Galpha protein thus providing a link between the signaling machinery at the plasma membrane and the downstream kinase.
Document information provided by NCBI PubMed
Text Mining Data
Dashed line = No text mining data
Manually curated Databases
-
IRef Biogrid Interaction:
PRKAR1A
—
AKAP10
(direct interaction, pull down)
-
IRef Biogrid Interaction:
PRKAR1A
—
AKAP10
(direct interaction, two hybrid)
-
IRef Hprd Interaction:
PRKAR1A
—
AKAP10
(in vitro)
-
IRef Hprd Interaction:
PRKAR1A
—
AKAP10
(two hybrid)
-
IRef Hprd Interaction:
PRKAR2B
—
AKAP10
(in vitro)
-
IRef Hprd Interaction:
AKAP10
—
PRKAR2A
(in vitro)
-
IRef Ophid Interaction:
PRKAR2B
—
AKAP10
(aggregation, confirmational text mining)
-
IRef Ophid Interaction:
PRKAR2A
—
AKAP10
(aggregation, confirmational text mining)
-
Reactome Reaction:
AKAP10
→
PRKAR1A
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKAR1B
(reaction)
-
Reactome Reaction:
AKAP1
→
PRKAR1B
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKACG
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKACG
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKACB
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKACB
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKAR1B
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKACG
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKAR1A
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKACA
(reaction)
-
Reactome Reaction:
AKAP1
→
PRKAR2B
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKAR2A
(reaction)
-
Reactome Reaction:
AKAP1
→
PRKACB
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKAR2A
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKACA
(indirect_complex)
-
Reactome Reaction:
AKAP10
→
PRKAR2B
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKAR1A
(indirect_complex)
-
Reactome Reaction:
AKAP10
→
PRKACG
(reaction)
-
Reactome Reaction:
AKAP1
→
PRKAR2A
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKAR1A
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKACB
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKAR2B
(indirect_complex)
-
Reactome Reaction:
AKAP10
→
PRKACA
(reaction)
-
Reactome Reaction:
AKAP1
→
PRKAR2B
(reaction)
-
Reactome Reaction:
AKAP10
→
PRKAR1B
(indirect_complex)
-
Reactome Reaction:
AKAP1
→
PRKACA
(indirect_complex)
-
Reactome Reaction:
AKAP10
→
PRKAR2A
(indirect_complex)
In total, 14 gene pairs are associated to this article in curated databases