Gene interactions and pathways from curated databases and text-mining
J Biol Chem 1998, PMID: 9765275

The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins.

Rietzler, M; Bittner, M; Kolanus, W; Schuster, A; Holzmann, B

Integrins of the beta7 subfamily, alpha4 beta7 and alphaE beta7, contribute to lymphocyte homing and to the development of protective or autoreactive immune responses at mucosal sites. The beta subunits of integrins are considered important for regulation of stimulated cell adhesion and adhesion-dependent signal transduction. Using a yeast interaction trap screen, a human WD repeat protein, termed WAIT-1, was isolated that interacts with the integrin beta7 cytoplasmic tail and is homologous to mouse EED and Drosophila ESC proteins. WAIT-1 also binds to the cytoplasmic domains of alpha4 and alphaE but not to those of integrin beta1, beta2, and alphaL subunits. Association of WAIT-1 and beta7-integrin was confirmed by coprecipitation from transiently transfected 293 cells. The binding site for WAIT-1 was mapped to a short membrane-proximal region of the beta7 cytoplasmic tail with Tyr-735 being of critical importance. Northern blot analysis revealed multiple WAIT-1-related transcripts with differential expression in circulating leukocytes, tissue-resident cells of diverse origin, and lymphoid malignancies. These results suggest that WAIT-1, together with the recently identified RACK1, may define a novel subfamily of WD repeat proteins that interact with distinct subsets of integrin cytoplasmic tails and may act as specific regulators of integrin function.

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Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Bind Interaction: ITGAE — EED
  • IRef Bind Interaction: EED — ITGB7
  • IRef Bind_translation Interaction: ITGAE — EED (two hybrid)
  • IRef Bind_translation Interaction: EED — ITGB7 (two hybrid)
  • IRef Bind_translation Interaction: EED — ITGB7 (affinity chromatography technology)
  • IRef Bind_translation Interaction: CYTH1 — ITGB2 (two hybrid)
  • IRef Bind_translation Interaction: ITGA4 — EED (two hybrid)
  • IRef Biogrid Interaction: ITGA4 — EED (direct interaction, two hybrid)
  • IRef Biogrid Interaction: CYTH1 — ITGB2 (direct interaction, two hybrid)
  • IRef Biogrid Interaction: ITGB7 — EED (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ITGB7 — EED (direct interaction, pull down)
  • IRef Biogrid Interaction: ITGB7 — EED (direct interaction, two hybrid)
  • IRef Biogrid Interaction: ITGAE — EED (direct interaction, two hybrid)
  • IRef Hprd Interaction: ITGA4 — EED (two hybrid)
  • IRef Hprd Interaction: ITGA4 — EED (in vitro)
  • IRef Hprd Interaction: ITGA4 — EED (in vivo)
  • IRef Hprd Interaction: ITGB7 — EED (two hybrid)
  • IRef Hprd Interaction: ITGB7 — EED (in vitro)
  • IRef Hprd Interaction: ITGB7 — EED (in vivo)
  • IRef Hprd Interaction: ITGAE — EED (two hybrid)
  • IRef Hprd Interaction: GNB2L1 — ITGB7 (two hybrid)
  • IRef Hprd Interaction: GNB2L1 — ITGB7 (in vivo)
  • IRef Hprd Interaction: GNB2L1 — ITGB7 (in vitro)
  • IRef Innatedb Interaction: ITGA4 — EED (unknown, -)
  • IRef Innatedb Interaction: EED — ITGB7 (unknown, -)
  • IRef Innatedb Interaction: ITGAE — EED (unknown, -)
  • IRef Ophid Interaction: ITGA4 — EED (aggregation, confirmational text mining)
  • IRef Ophid Interaction: EED — ITGB7 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: ITGAE — EED (aggregation, confirmational text mining)
In total, 5 gene pairs are associated to this article in curated databases