Gene interactions and pathways from curated databases and text-mining
Genes Dev 1998, PMID: 9808620

Activation of Rac1 by a Crk SH3-binding protein, DOCK180.

Kiyokawa, E; Hashimoto, Y; Kobayashi, S; Sugimura, H; Kurata, T; Matsuda, M

DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of CrkII and p130(Cas) enhanced this DOCK180-dependent activation of Rac1. Furthermore, we observed direct binding of DOCK180 to Rac1, but not to RhoA or Cdc42Hs. Dominant-negative Rac1 suppressed DOCK180-induced membrane spreading. These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling.

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Text Mining Data

Rac1 → DOCK180: " Activation of Rac1 by a Crk SH3 binding protein, DOCK180 "

Rac1 → DOCK180: " Coexpression of CrkII and p130 ( Cas ) enhanced this DOCK180 dependent activation of Rac1 "

Rac1 → p130: " Coexpression of CrkII and p130 ( Cas ) enhanced this DOCK180 dependent activation of Rac1 "

Rac1 → DOCK180: " These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling "

Manually curated Databases

In total, 34 gene pairs are associated to this article in curated databases