Genes Dev 1998,
PMID: 9808620
Kiyokawa, E; Hashimoto, Y; Kobayashi, S; Sugimura, H; Kurata, T; Matsuda, M
DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of CrkII and p130(Cas) enhanced this DOCK180-dependent activation of Rac1. Furthermore, we observed direct binding of DOCK180 to Rac1, but not to RhoA or Cdc42Hs. Dominant-negative Rac1 suppressed DOCK180-induced membrane spreading. These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling.
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Text Mining Data
Rac1 → DOCK180: "
Activation of
Rac1 by a Crk SH3 binding protein,
DOCK180
"
Rac1 → DOCK180: "
Coexpression of CrkII and p130 ( Cas ) enhanced this DOCK180 dependent activation of Rac1
"
Rac1 → p130: "
Coexpression of CrkII and p130 ( Cas ) enhanced this DOCK180 dependent activation of Rac1
"
Rac1 → DOCK180: "
These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling
"
Manually curated Databases
-
IRef Biogrid Interaction:
DOCK1
—
RAC1
(direct interaction, pull down)
-
IRef Hprd Interaction:
DOCK1
—
RAC1
(in vivo)
-
IRef Hprd Interaction:
DOCK1
—
RAC1
(in vitro)
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
None
→
RAC1/GDP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
None
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
None
→
FAK/Src-Yes-Fyn/p130 CAS/CRK/DOCK1/ELMO1 complex (PTK2-YES1_SRC_FYN-BCAR1-CRK-DOCK1-ELMO1)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
None
→
None
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
RAC1/GDP complex (RAC1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
RAC1/GDP complex (RAC1)
→
FAK/Src-Yes-Fyn/p130 CAS/CRK/DOCK1/ELMO1 complex (PTK2-YES1_SRC_FYN-BCAR1-CRK-DOCK1-ELMO1)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
RAC1/GDP complex (RAC1)
→
None
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
RAC1/GTP complex (RAC1)
→
None
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Signaling events mediated by focal adhesion kinase:
FAK/Src-Yes-Fyn/p130 CAS/CRK/DOCK1/ELMO1 complex (PTK2-YES1_SRC_FYN-BCAR1-CRK-DOCK1-ELMO1)
→
None
(modification, activates)
Evidence: mutant phenotype, physical interaction
In total, 34 gene pairs are associated to this article in curated databases