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LIPE — PRKAR1B
Pathways - manually collected, often from reviews:
-
KEGG Insulin signaling pathway:
PRKACA/PRKACB/PRKACG/PRKAR1A/PRKAR1B/PRKAR2A/PRKAR2B/PRKX
→
LIPE
(protein-protein, activation)
Text-mined interactions from Literome
Souza et al., J Biol Chem 2002
:
Activation of cyclic AMP dependent
protein kinase (PKA) results in the phosphorylation of Peri A and
hormone-sensitive lipase (HSL) , the predominant lipase in adipocytes, with concurrent stimulation of adipocyte lipolysis
He et al., Biochim Biophys Acta 2006
:
Lipolysis is primarily regulated by
protein kinase A (PKA) , which phosphorylates perilipin and hormone-sensitive lipase (HSL), and
causes translocation of
HSL from cytosol to lipid droplets in adipocytes
Miyoshi et al., J Biol Chem 2006
:
Using adipocytes retrovirally engineered from murine embryonic fibroblasts of perilipin null mice ( Peri-/- MEF ), we demonstrate by cell fractionation and confocal microscopy that up to 50 % of cellular HSL is LD-associated in the basal state and that
PKA stimulated
HSL translocation is fully supported by adenoviral expression of a mutant perilipin lacking all six PKA sites ( Peri Adelta1-6 ) ...
PKA stimulated
HSL translocation was confirmed in differentiated brown adipocytes from perilipin null mice expressing an adipose-specific Peri Adelta1-6 transgene ... Thus,
PKA induced
HSL translocation was independent of perilipin phosphorylation
Granneman et al., J Biol Chem 2007
:
Double label immunofluorescence analysis of 3T3-L1 adipocytes indicated that
PKA activation
increases the translocation of
hormonesensitive lipase (HSL) to perilipin A (Plin) containing droplets and increases the colocalization of adipose tissue triglyceride lipase ( Atgl ) with its coactivator, Abhd5
Krintel et al., FEBS J 2009
:
The exact molecular events of
PKA mediated activation of
HSL in vitro are yet to be determined, but increases in both Vmax and S0.5 seem to be involved, as recently shown for human HSL
Djouder et al., EMBO J 2010
:
TAG resynthesis is associated with high-energy consumption, which when inordinate, leads to increased AMPK activity that acts to restrain hydrolysis of TAGs by inhibiting
PKA mediated activation of
HSL
Lee et al., Am J Physiol Endocrinol Metab 2012
(Insulin Resistance) :
Cotreatment with DEX blocked TNF induction of basal lipolysis and insulin resistance by antagonizing TNF stimulation of
PKA mediated phosphorylation of
hormone-sensitive lipase (HSL) at Ser ( 563 ) and Ser ( 660 ) and perilipin
Mulder et al., Diabetes 1999
:
Because
HSL in adipocytes is
activated by cAMP dependent
protein kinase (PKA) , PKA regulated triglyceride hydrolysis in beta-cells may participate in the regulation of insulin secretion, possibly by providing a lipid derived signal, e.g., long-chain acyl-CoA and diacylglycerol