◀ Back to BRCA1
BRCA1 — RAD50
Pathways - manually collected, often from reviews:
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A)
(modification, collaborate)
Wang et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
ABRA1 (FAM175A)
(modification, collaborate)
Wang et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A)
→
ABRA1 (FAM175A)
(modification, collaborate)
Wang et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database BARD1 signaling events:
ATM (ATM)
→
BRCA1/BARD1/CTIP/M/R/N Complex complex (MRE11A-RAD50-NBN-BRCA1-BARD1-RBBP8)
(modification, activates)
Greenberg et al., Genes Dev 2006
Evidence: assay, physical interaction
-
NCI Pathway Database BARD1 signaling events:
BRCA1/BARD1/CTIP/M/R/N Complex complex (MRE11A-RAD50-NBN-BRCA1-BARD1-RBBP8)
→
BRCA1/BARD1/CTIP complex (BRCA1-BARD1-RBBP8)
(modification, collaborate)
Greenberg et al., Genes Dev 2006
Evidence: assay, physical interaction
-
NCI Pathway Database BARD1 signaling events:
BRCA1/BARD1/CTIP/M/R/N Complex complex (MRE11A-RAD50-NBN-BRCA1-BARD1-RBBP8)
→
M/R/N Complex complex (MRE11A-RAD50-NBN)
(modification, collaborate)
Greenberg et al., Genes Dev 2006
Evidence: assay, physical interaction
-
NCI Pathway Database BARD1 signaling events:
BRCA1/BARD1/CTIP complex (BRCA1-BARD1-RBBP8)
→
M/R/N Complex complex (MRE11A-RAD50-NBN)
(modification, collaborate)
Greenberg et al., Genes Dev 2006
Evidence: assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1)
(modification, collaborate)
Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
CtBP/CtIP complex (RBBP8)
(modification, collaborate)
Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8)
(modification, collaborate)
Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1)
→
BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8)
(modification, collaborate)
Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1)
→
RAP80 (UIMC1)
(modification, collaborate)
Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1)
→
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A)
(modification, collaborate)
Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database ATM pathway:
RAP80 (UIMC1)
→
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A)
(modification, collaborate)
Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
SMC1 (SMC1A)
(modification, activates)
Kim et al., Genes Dev 2002, Yazdi et al., Genes Dev 2002, Kitagawa et al., Genes Dev 2004
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
SMC3 (SMC3)
(modification, activates)
Luo et al., J Biol Chem 2008
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1)
(modification, collaborate)
Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
CtBP/CtIP complex (RBBP8)
(modification, collaborate)
Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1)
→
BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8)
(modification, collaborate)
Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database ATM pathway:
MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1)
→
BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8)
(modification, collaborate)
Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
Evidence: mutant phenotype, assay, physical interaction
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Bind Interaction:
BRCA1
—
RAD50
Zhong et al., Science 1999*, Wang et al., Genes Dev 2000, Chiba et al., J Biol Chem 2001*
-
IRef Bind Interaction:
Complex of 12 proteins
Wang et al., Genes Dev 2000
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(coimmunoprecipitation)
Zhong et al., Science 1999*, Wang et al., Genes Dev 2000
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(affinity chromatography technology)
Zhong et al., Science 1999*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(two hybrid)
Zhong et al., Science 1999*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(coimmunoprecipitation)
Zhong et al., Science 1999*, Chiba et al., J Biol Chem 2001*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(unspecified method)
Zhong et al., Science 1999*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(cosedimentation through density gradient)
Zhong et al., Science 1999*, Chiba et al., J Biol Chem 2001*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(coimmunoprecipitation)
Zhong et al., Science 1999*
-
IRef Bind_translation Interaction:
BRCA1
—
RAD50
(unspecified method)
Zhong et al., Science 1999*, Wang et al., Genes Dev 2000
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(physical association, affinity chromatography technology)
Chen et al., J Biol Chem 2008*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(association, biochemical)
Wang et al., Genes Dev 2000
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(association, biochemical)
Chiba et al., J Biol Chem 2001*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(direct interaction, two hybrid)
Zhong et al., Science 1999*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(physical association, affinity chromatography technology)
Zhong et al., Science 1999*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(direct interaction, pull down)
Zhong et al., Science 1999*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(colocalization, imaging technique)
Zhong et al., Science 1999*
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(physical association, affinity chromatography technology)
Oishi et al., J Biol Chem 2006
-
IRef Biogrid Interaction:
BRCA1
—
RAD50
(physical association, affinity chromatography technology)
Greenberg et al., Genes Dev 2006
-
MIPS CORUM BRCA1-RAD50-MRE11-NBS1 complex:
BRCA1-RAD50-MRE11-NBS1 complex complex (BRCA1-MRE11A-NBN-RAD50)
Chiba et al., J Biol Chem 2001*
-
MIPS CORUM RAD50-BRCA1 complex:
RAD50-BRCA1 complex complex (BRCA1-RAD50)
Chiba et al., J Biol Chem 2001*
-
MIPS CORUM BRCA1-BARD1-BACH1-DNA damage complex II:
BRCA1-BARD1-BACH1-DNA damage complex II complex (BACH1-BARD1-BRCA1-MRE11A-NBN-RAD50-RBBP8-TOPBP1)
Greenberg et al., Genes Dev 2006
-
MIPS CORUM BASC complex (BRCA1-associated genome surveillance complex):
BASC complex (BRCA1-associated genome surveillance complex) complex (ATM-BLM-BRCA1-MLH1-MRE11A-MSH2-MSH6-NBN-RAD50-RFC1-RFC2-RFC4)
Wang et al., Genes Dev 2000
-
MIPS CORUM BASC (Ab C-20) complex (BRCA1-associated genome surveillance complex):
BASC (Ab C-20) complex (BRCA1-associated genome surveillance complex) complex (BRCA1-MSH6-RAD50)
Wang et al., Genes Dev 2000
-
MIPS CORUM BRCA1-RAD50-MRE11-NBS1 complex:
BRCA1-RAD50-MRE11-NBS1 complex complex (BRCA1-MRE11A-NBN-RAD50)
Zhong et al., Science 1999*
-
IRef Corum Interaction:
Complex of 13 proteins
(association, affinity chromatography technology)
Chiba et al., J Biol Chem 2001*
-
IRef Corum Interaction:
BRCA1
—
RAD50
(association, anti tag coimmunoprecipitation)
Chiba et al., J Biol Chem 2001*
-
IRef Corum Interaction:
BRCA1
—
RAD50
(association, ion exchange chromatography)
Chiba et al., J Biol Chem 2001*
-
IRef Corum Interaction:
Complex of 37 proteins
(association, coimmunoprecipitation)
Wang et al., Genes Dev 2000
-
IRef Corum Interaction:
Complex of 17 proteins
(association, cosedimentation through density gradient)
Greenberg et al., Genes Dev 2006
-
IRef Corum Interaction:
Complex of BRCA1-MSH6-RAD50
(association, anti tag coimmunoprecipitation)
Wang et al., Genes Dev 2000
-
IRef Hprd Interaction:
Complex of 73 proteins
(in vivo)
Oishi et al., J Biol Chem 2006
-
IRef Hprd Interaction:
Complex of 201 proteins
(in vivo)
Wang et al., Genes Dev 2000, Yazdi et al., Genes Dev 2002
-
IRef Intact Interaction:
Complex of 11 proteins
(association, anti bait coimmunoprecipitation)
Oishi et al., J Biol Chem 2006
-
IRef Intact Interaction:
Complex of RAD50-CHEK2-TP53BP1-BRCA1-GINS2
(association, anti bait coimmunoprecipitation)
Matsuoka et al., Science 2007
-
IRef Ophid Interaction:
BRCA1
—
RAD50
(aggregation, confirmational text mining)
Wang et al., Genes Dev 2000
-
IRef Ophid Interaction:
BRCA1
—
RAD50
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
-
IRef Ophid Interaction:
BRCA1
—
RAD50
(aggregation, confirmational text mining)
Zhong et al., Science 1999*
Text-mined interactions from Literome
Paull et al., Proc Natl Acad Sci U S A 2001
:
As a result of this binding,
Brca1 inhibits the nucleolytic activities of the
Mre11/Rad50/Nbs1 complex , an enzyme implicated in numerous aspects of double-strand break repair
Yang et al., PloS one 2008
(Adenocarcinoma...) :
Oncogenic
K-ras induced
BASC accumulation and tumor growth were blocked by treatment with a small molecule PI3K inhibitor and enhanced by inactivation of phosphatase and tensin homologue deleted from chromosome 10, a negative regulator of PI3K
Dovey et al., Proc Natl Acad Sci U S A 2008
(Cell Transformation, Neoplastic...) :
Abrogated lung cancer development and
BASC self-renewal occur partially in a
p19(ARF) dependent manner
Ballal et al., J Biol Chem 2009
:
Our findings further suggest that
Rad50 is required to localize BRCA1 at the telomere and that the association of
BRCA1 with Rad50 does not
require DNA ... Our findings suggest that BRCA1 is recruited to the telomere in a
Rad50 dependent manner and that
BRCA1 may regulate telomere length and stability, in part through its presence at the telomere