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PAK1 — RAF1

Pathways - manually collected, often from reviews:

• BioCarta influence of ras and rho proteins on g1 to s transition: PAK1 → RAF1 (modification, activates)
• KEGG Focal adhesion: PAK1/PAK2/PAK3/PAK4/PAK6/PAK7 → RAF1 (protein-protein, phosphorylation)
• WikiPathways Focal Adhesion: PAK1/PAK3/PAK2/PAK4/PAK7/PAK6 → ARAF/BRAF/RAF1 (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: RAF1 — PAK1 (physical association, affinity chromatography technology) Zang et al., J Biol Chem 2002*
• IRef Biogrid Interaction: RAF1 — PAK1 (direct interaction, pull down) Zang et al., J Biol Chem 2002*
• IRef Biogrid Interaction: RAF1 — PAK1 (direct interaction, enzymatic study) Zang et al., J Biol Chem 2002*
• IRef Hprd Interaction: RAF1 — PAK1 (in vitro) Mason et al., EMBO J 1999, Zang et al., J Biol Chem 2002*, Tran et al., J Biol Chem 2003*, Diaz et al., Mol Cell Biol 1997*, King et al., Nature 1998*
• IRef Hprd Interaction: RAF1 — PAK1 (in vivo) Mason et al., EMBO J 1999, Zang et al., J Biol Chem 2002*, Tran et al., J Biol Chem 2003*, Diaz et al., Mol Cell Biol 1997*, King et al., Nature 1998*
• IRef Intact Interaction: RAF1 — PAK1 (phosphorylation reaction, protein kinase assay) Park et al., FEBS Lett 2006*
• IRef Ophid Interaction: RAF1 — PAK1 (aggregation, confirmational text mining) Zang et al., J Biol Chem 2002*
• IRef Ophid Interaction: RAF1 — PAK1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Sun et al., Curr Biol 2000 : Thus, Raf-1 activation by Ras is achieved through a combination of both physical interaction and indirect mechanisms involving the activation of a second Ras effector, PI 3-kinase, which directs Pak mediated regulatory phosphorylation of Raf-1
King et al., FEBS Lett 2001 : Phosphorylation site specificity of the Pak mediated regulation of Raf-1 and cooperativity with Src
Zang et al., J Biol Chem 2002 : Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1
Beeser et al., J Biol Chem 2005 : Inhibition of Pak reduced the Ser ( 338 ) phosphorylation of c-Raf in response to both PDGF and EGF ; however, in the case of EGF, the reduction in Ser ( 338 ) phosphorylation was not accompanied by a significant decrease in c-Raf activity
Wu et al., J Cell Biochem 2008 : We observed that all group II PAKs were efficient serine 338 kinases, although only PAK1 and PAK5 significantly stimulated Raf-1 kinase activity