◀ Back to RAC1
NCKAP1 — RAC1
Pathways - manually collected, often from reviews:
-
BioCarta y branching of actin filaments:
WAVE1-3/Nap/PIR/Abl2/HSPC complex (WASF2-NCKAP1-PIR-ABL2-PSMA7)
→
RAC1
(modification, collaborate)
-
BioCarta y branching of actin filaments:
WAVE1-3/Nap/PIR/Abl2/HSPC complex (WASF2-NCKAP1-PIR-ABL2-PSMA7)
→
RAC1/PIR/Abl2/Nap complex (RAC1-PIR-ABL2-NCKAP1)
(modification, collaborate)
-
BioCarta y branching of actin filaments:
RAC1
→
RAC1/PIR/Abl2/Nap complex (RAC1-PIR-ABL2-NCKAP1)
(modification, collaborate)
-
BioCarta y branching of actin filaments:
WAVE1-3/HSPC complex (WASF2-PSMA7)
→
RAC1/PIR/Abl2/Nap complex (RAC1-PIR-ABL2-NCKAP1)
(modification, collaborate)
-
KEGG Regulation of actin cytoskeleton:
RAC1/RAC2/RAC3
→
Complex of ABI2-CYFIP1-CYFIP2-NCKAP1-NCKAP1L
(protein-protein, inhibition)
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
Nap1 (NCKAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Yamazaki et al., J Cell Sci 2007, Ryu et al., Mol Cell Biol 2009
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
RAC1/GTP complex (RAC1)
→
ABI1/Sra1/Nap1 complex (ABI1-CYFIP2-NCKAP1)
(modification, activates)
Yamazaki et al., J Cell Sci 2007, Ryu et al., Mol Cell Biol 2009
Evidence: mutant phenotype, assay, physical interaction
-
Reactome Reaction:
NCKAP1
→
RAC1
(indirect_complex)
Leng et al., Proc Natl Acad Sci U S A 2005, Suetsugu et al., J Cell Biol 2006, Nakanishi et al., J Biochem 2007, Mendoza et al., Mol Cell 2011, Miki et al., EMBO J 1998
-
Reactome Reaction:
NCKAP1
→
RAC1
(reaction)
Suetsugu et al., J Cell Biol 2006, Miki et al., EMBO J 1998
-
WikiPathways Regulation of Actin Cytoskeleton:
RAC1/RAC3/RAC2
→
NCKAP1/CYFIP2/ABI2
(mim-inhibition)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
RAC1
—
NCKAP1
(direct interaction, pull down)
Kitamura et al., Biochem J 1997*
-
IRef Biogrid Interaction:
RAC1
—
NCKAP1
(physical association, affinity chromatography technology)
Kitamura et al., Biochem J 1997*
-
IRef Dip Interaction:
Complex of ABI2-RAC1-BRK1-CYFIP1-WASF1-NCKAP1
(pull down)
Ismail et al., Nature structural & molecular biology 2009
-
IRef Dip Interaction:
Complex of RAC1-RAC1-CYFIP1-CYFIP1-NCKAP1-NCKAP1
(pull down)
Ismail et al., Nature structural & molecular biology 2009
-
IRef Dip Interaction:
RAC1
—
NCKAP1
(physical association, pull down)
Chen et al., Nature 2010*
-
IRef Dip Interaction:
RAC1
—
NCKAP1
(physical association, pull down)
Ismail et al., Nature structural & molecular biology 2009
-
IRef Hprd Interaction:
Complex of 51 proteins
(in vivo)
Eden et al., Nature 2002, Mayne et al., Eur J Immunol 2004*, Witke et al., EMBO J 1998
-
IRef Hprd Interaction:
RAC1
—
NCKAP1
(in vivo)
Kitamura et al., Biochem J 1997*, Kobayashi et al., J Biol Chem 1998*
-
IRef Hprd Interaction:
RAC1
—
NCKAP1
(in vitro)
Kitamura et al., Biochem J 1997*, Kobayashi et al., J Biol Chem 1998*
-
IRef Intact Interaction:
RAC1
—
NCKAP1
(physical association, pull down)
Kitamura et al., Biochem J 1997*
-
IRef Ophid Interaction:
RAC1
—
NCKAP1
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
-
IRef Ophid Interaction:
RAC1
—
NCKAP1
(aggregation, confirmational text mining)
Kitamura et al., Biochem J 1997*
Text-mined interactions from Literome
Miki et al., Biochem Biophys Res Commun 2002
:
Interestingly, the intramolecular interaction is interrupted by the binding of WAVE2 and full-length IRSp53 associates with
Rac in the
presence of
WAVE2
Oikawa et al., Nat Cell Biol 2004
:
Production of PtdIns ( 3,4,5 ) P ( 3 ) at the cell membrane by myristoylated phosphatidylinositol-3-OH kinase ( PI(3)K ) is sufficient to recruit
WAVE2 in the
presence of dominant negative
Rac and latrunculin, demonstrating that PtdIns ( 3,4,5 ) P ( 3 ) alone is able to recruit WAVE2
Suetsugu et al., J Cell Biol 2006
:
Purified
WAVE2 and purified WAVE2 complex were activated by IRSp53 in a
Rac dependent manner with PIP ( 3 ) -containing liposomes ... Therefore, IRSp53 optimizes the activity of the
WAVE2 complex in the
presence of activated
Rac and PIP ( 3 )
Harmon et al., PLoS Pathog 2010
(Astrocytoma...) :
Rac and the tyrosine kinase Abl then
activate the
Wave2 complex and promote Arp2/3 dependent actin polymerization