Wong et al., J Exp Med 2003
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PIP2 formation is regulated by Arf6 Aikawa et al., Methods Enzymol 2005
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Long-term expression of a constitutively active Arf6 mutant in cells can lead to the depletion of PIP2 from the plasma membrane, its accumulation in intracellular vacuoles, and the inhibition of PIP2 dependent membrane trafficking at the plasma membrane
Randazzo et al., J Biol Chem 1994
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PIP2 alone stimulated ARF GAP activity up to 30-fold with a half-maximal effect at 100-300 microM but had no effect on the GTPase rate of ARF alone
Kahn et al., J Lipid Mediat Cell Signal 1996
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The previously described requirements for PIP2 in the ARF stimulated phospholipase D ( PLD ) activity and ARF GTPase activating protein ( ARF GAP ) assays provide the basis for a model in which PIP2 acts as a cofactor in one or more ARF pathways