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RHOA — SMURF1

Pathways - manually collected, often from reviews:

• NCI Pathway Database TGF-beta receptor signaling: RhoA (RHOA) → TGFB/TGFBR2/TGFBR1/Par6/occludin/SMURF1 complex (TGFBR1-PARD6A-OCLN-SMURF1-TGFBR2) (proteasomal ubiquitin-dependent protein catabolic process, collaborate)
  Ozdamar et al., Science 2005
  Evidence: mutant phenotype
• Reactome Reaction: RHOA → SMURF1 (indirect_complex) Ozdamar et al., Science 2005
• Reactome Reaction: RHOA → SMURF1 (reaction) Ozdamar et al., Science 2005

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: RHOA — SMURF1 (direct interaction, pull down) Asanuma et al., Nat Cell Biol 2006*
• IRef Biogrid Interaction: RHOA — SMURF1 (direct interaction, enzymatic study) Tian et al., FEBS Lett 2011*
• IRef Biogrid Interaction: RHOA — SMURF1 (physical association, affinity chromatography technology) Tian et al., FEBS Lett 2011*
• IRef Biogrid Interaction: RHOA — SMURF1 (direct interaction, pull down) Tian et al., FEBS Lett 2011*
• IRef Hprd Interaction: RHOA — SMURF1 (in vivo) Bryan et al., FEBS Lett 2005*, Wang et al., Methods Enzymol 2006*
• IRef Hprd Interaction: RHOA — SMURF1 (in vitro) Bryan et al., FEBS Lett 2005*, Wang et al., Methods Enzymol 2006*
• IRef Intact Interaction: SMURF1 — RHOA (physical association, anti tag coimmunoprecipitation) Bryan et al., FEBS Lett 2005*
• IRef Intact Interaction: SMURF1 — RHOA (physical association, anti tag coimmunoprecipitation) Tian et al., FEBS Lett 2011*
• IRef Intact Interaction: SMURF1 — RHOA (physical association, pull down) Tian et al., FEBS Lett 2011*

Text-mined interactions from Literome

Zhang et al., Cell cycle (Georgetown, Tex.) 2004 : Smurf1 thus provides a link between the control of cell polarity and ubiquitin mediated RhoA degradation during directional cell movements
Bryan et al., FEBS Lett 2005 : Interestingly, Smurf1 overexpression in Neuro2a cells dramatically reduces RhoA protein levels during dibutyric cyclic AMP, but not retinoic acid induced neurite outgrowth ... This Smurf1 dependent reduction in RhoA protein levels was abrogated using the general proteasome inhibitor MG132, suggesting that RhoA is targeted for ubiquitination and degradation via Smurf1
Sahai et al., J Cell Biol 2007 (Neoplasm Invasiveness...) : We report that Smurf1 dependent RhoA degradation in tumor cells results in the down-regulation of Rho kinase ( ROCK ) activity and myosin light chain 2 (MLC2) phosphorylation at the cell periphery
Crose et al., J Biol Chem 2009 : CCM2 binding of Smurf1 increases Smurf1 mediated degradation of RhoA
Ogunjimi et al., J Biol Chem 2010 : Analysis of mutants of the HECT UBS reveal that interfering with the UBS surface blocked Smurf dependent degradation of its substrate RhoA in cells
Ding et al., Curr Top Med Chem 2011 : In this review, we focus on how ubiquitin signaling guides the fate and function of Rho GTPases and their regulators, especially how the E3 ubiquitin ligase Smurf1 regulates cell polarity and motility through targeting RhoA for ubiquitination and degradation