◀ Back to E2F1

E2F1 — SP1

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: SP1 → E2F1 (increases, E2F1 Activity)
  Evidence: Sp1 and E2F binding sites are both essential for activation of the murine thymidine kinase promoter (Karlseder et al., 1996). The promoter was activated when the Sp1 and E2F sites were separated by 6 or 10 bp but not when they were separated by 20 bp. The DHFR promoter was strongly activated by Sp1 alone but hardly at all by E2F1 alone (cotransfection in insect cells) (Lin et al., 1996a). E2F1, however, enhanced the activating ability of Sp1, even in the absence of a functional E2F binding...
• FastForward regulation: SP1, NFYA → E2F1 (transcriptional regulation, unknown) Wang et al., Mol Endocrinol 1999*, Bossa et al., FEBS Lett 1976*
  Evidence: DNABINDING
• FastForward regulation: SP1 → E2F1 (transcriptional regulation, unknown)
  Evidence: DNABINDING
• FastForward regulation: SP1 → E2F1 (transcriptional regulation, unknown) Wang et al., Mol Endocrinol 1999*, Bossa et al., FEBS Lett 1976*
  Evidence: DNABINDING
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Takahashi et al., Genes Dev 2000, Johnson et al., Genes Dev 1994, Hsiao et al., Genes Dev 1994, Hurford et al., Genes Dev 1997, Ferreira et al., Proc Natl Acad Sci U S A 1998
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Gartel et al., Oncogene 1998, Hiyama et al., Oncogene 1997
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Di Stefano et al., EMBO J 2003, Christensen et al., Nucleic Acids Res 2005, Johnson et al., Genes Dev 1994, Hsiao et al., Genes Dev 1994
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Johnson et al., Genes Dev 1994, Hsiao et al., Genes Dev 1994, Gaubatz et al., Proc Natl Acad Sci U S A 1998
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database Direct p53 effectors: E2F1-3/DP complex (E2F3_E2F2_E2F1-TFDP1) → SP1 (SP1) (transcription, activates) Bist et al., Biochemistry 2000
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Di Fiore et al., J Biol Chem 1999*
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Wu et al., Nature 2001, Li et al., Mol Cell Biol 1994, Karlseder et al., Mol Cell Biol 1996, Cartwright et al., Oncogene 1998
  Evidence: mutant phenotype, reporter gene, physical interaction, other species
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Wang et al., Nat Cell Biol 2006, Johnson et al., Genes Dev 1994, Hsiao et al., Genes Dev 1994
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: SP1 (SP1) → E2F1 (E2F1) (transcription, activates) Wang et al., Nat Cell Biol 2006, Johnson et al., Genes Dev 1994, Hsiao et al., Genes Dev 1994
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Blais et al., J Biol Chem 2002*
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → SP1 (SP1) (transcription, activates) Wu et al., Nature 2001, Li et al., Mol Cell Biol 1994
  Evidence: mutant phenotype, reporter gene, other species
• Reactome Reaction: SP1 → E2F1 (reaction) Parisi et al., Biochem Biophys Res Commun 2002*
• Reactome Reaction: SP1 → E2F1 (indirect_complex) Parisi et al., Biochem Biophys Res Commun 2002*

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: SP1 — E2F1 Karlseder et al., Mol Cell Biol 1996
• IRef Bind_translation Interaction: SP1 — E2F1 (unspecified method) Karlseder et al., Mol Cell Biol 1996
• IRef Biogrid Interaction: SP1 — E2F1 (direct interaction, pull down) Doetzlhofer et al., Mol Cell Biol 1999*
• IRef Biogrid Interaction: SP1 — E2F1 (direct interaction, pull down) Karlseder et al., Mol Cell Biol 1996
• IRef Biogrid Interaction: SP1 — E2F1 (direct interaction, pull down) Rotheneder et al., J Mol Biol 1999*
• IRef Biogrid Interaction: SP1 — E2F1 (direct interaction, pull down) Lin et al., Mol Cell Biol 1996*
• IRef Biogrid Interaction: SP1 — E2F1 (physical association, affinity chromatography technology) Rotheneder et al., J Mol Biol 1999*
• IRef Biogrid Interaction: SP1 — E2F1 (physical association, affinity chromatography technology) Lin et al., Mol Cell Biol 1996*
• IRef Hprd Interaction: SP1 — E2F1 (in vivo) Rotheneder et al., J Mol Biol 1999*, Opitz et al., Cancer Res 2000*
• IRef Hprd Interaction: SP1 — E2F1 (in vitro) Rotheneder et al., J Mol Biol 1999*, Opitz et al., Cancer Res 2000*
• IRef Intact Interaction: SP1 — E2F1 (direct interaction, pull down) Karlseder et al., Mol Cell Biol 1996
• IRef Mppi Interaction: SP1 — E2F1 (copurification) Karlseder et al., Mol Cell Biol 1996
• IRef Ophid Interaction: SP1 — E2F1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: SP1 — E2F1 (aggregation, confirmational text mining) Rotheneder et al., J Mol Biol 1999*

Text-mined interactions from Literome

Wang et al., Mol Endocrinol 1999 (Breast Neoplasms) : Transcriptional activation of E2F1 gene expression by 17beta-estradiol in MCF-7 cells is regulated by NF-Y-Sp1/estrogen receptor interactions ... Thus, transcriptional activation of E2F1 gene expression in MCF-7 cells by E2 is regulated by multiprotein ER/Sp1-NF-Y interactions at GC-rich and two CCAAT elements in the proximal region of the E2F1 gene promoter
Nicolás et al., Biochem J 2003 : Whereas overexpression of NF-Y or E2F had an additive effect on Sp1 overexpression, the activation of Sp1 transcription due to Sp1 was counteracted by Sp3 overexpression
Tapias et al., Cell cycle (Georgetown, Tex.) 2008 : Among the known Sp1 interacting proteins, E2F-DP1 , Cyclin D1, Stat3 and Rb activated the Sp1 promoter, whereas p53 and NF kappaB inhibited it
Hazar-Rethinam et al., J Invest Dermatol 2011 : Transient transfection of E2F1-E2F6 indicated that E2F mediated reduction of Sp1 transcription was not responsible for E2F mediated suppression of squamous differentiation
Shao et al., Oncogene 1995 : Since Rb is a regulator of E2F and Sp1 mediated transcription, we have examined the effect of overexpression of cyclins on transcription mediated by E2F dependent adenovirus E2 promoter and by Sp1 in a cotransfection assay in 3T3 cells