◀ Back to IL1B
IL1B — NFKBIA
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Katsuyama et al., Endocrinology 1999
:
IL-1beta caused a rapid increase in phosphorylated IkappaB-alpha levels and subsequent transient decrease in
IkappaB-alpha levels, an inhibitor of NF-kappaB, as revealed by Western blot analysis using specific antibodies for phosphorylated and nonphosphorylated IkappaB-alpha
Laflamme et al., J Neurochem 1999
(Inflammation) :
Systemic LPS,
IL-1beta , and TNF-alpha
caused a rapid and transient transcriptional activation of
IkappaB alpha along the blood vessels of the entire brain ; the signal was very intense 30-60 min after the i.v. injections and returned to undetectable levels from 2 to 12 h depending on the challenge
Adcock et al., Br J Pharmacol 1999
:
No induction of the endogenous inhibitors of NF-kappaB, IkappaBalpha or I-kappaBbeta, was seen at 24 h and the ability of
IL-1beta to degrade and subsequently
induce IkappaBalpha was not altered by glucocorticoids
Lin et al., Mol Pharmacol 2000
:
IL-1beta caused the translocation of p65 NF-kappaB from cytosol to the nucleus as well as the degradation of
IkappaB-alpha in cytosol
Parikh et al., Shock 2000
(Adenocarcinoma...) :
Similarly, scavenging of free radicals and oxidants by pyrrolidine dithiocarbamate and dimethyl sulfoxide did not block
IL-1beta induced
IkappaBalpha degradation and NF-kappaB activation
Katsuyama et al., J Biochem 2001
:
Western blot analyses involving anti-IkappaB-alpha and anti-phospho IkappaB-alpha antibodies showed that
IL-1beta induced transient degradation of
IkappaB-alpha preceded by the rapid appearance of phosphorylated IkappaB-alpha, both of which were markedly blocked by N2733
Bian et al., Exp Eye Res 2001
(MAP Kinase Signaling System) :
Stimulation of hRPE with
IL-1beta and TNF-alpha
resulted in degradation of
IkappaB-alpha , nuclear translocation of NF-kappaB, and prominent increases in p38 and ERK1/2 phosphorylation for as little as 3 min
Ferlito et al., J Leukoc Biol 2001
(Leukemia, Monocytic, Acute...) :
In naive cells, LPS, TNF-alpha, and
IL-1beta induced
IkappaBalpha degradation, kinase phosphorylation, and NF-kappaB DNA binding
Jiang et al., Arterioscler Thromb Vasc Biol 2001
:
IkappaBbeta, but not
IkappaBalpha degradation,
induced by
IL-1beta was markedly attenuated when ERK activation was inhibited and could be partially responsible for the persistent NF-kappaB activation
Chen et al., J Biol Chem 2002
:
In human embryonic kidney 293 cells,
IL-1beta induces IkappaB kinase beta (IKKbeta) activation,
IkappaBalpha degradation, NF-kappaB transactivation, and weak Akt activation
Kabouridis et al., J Immunol 2002
(MAP Kinase Signaling System) :
In contrast, exogenously added TAT-srIkappaBalpha did not inhibit
IL-1beta induced activation of extracellular signal regulated kinase, c-Jun N-terminal kinase, or p38 mitogen activated protein kinases or the phosphorylation and degradation of endogenous
IkappaBalpha
Lin et al., J Pharm Pharmacol 2002
:
Treatment of cells with byakangelicol ( 50 microM ) or pyrrolidine dithiocarbamate ( PDTC ; 50 microM ) partially inhibited
IL-1beta induced degradation of
IkappaB-alpha in the cytosol, translocation of p65 NF-kappaB from the cytosol to the nucleus and the NF-kappaB-specific DNA-protein complex formation
Yang et al., Life Sci 2002
:
Treatment of cells with sphondin ( 50 microM ) or the NF-kappaB inhibitor, PDTC ( 50 microM ) partially inhibited
IL-1beta induced degradation of
IkappaB-alpha in the cytosol and translocation of p65 NF-kappaB from the cytosol to the nucleus
Lee et al., J Immunol 2003
:
In contrast,
IL-1 beta induced NF-kappa B activity and
I kappa B alpha degradation were not affected by BMP-7
Jiang et al., J Biol Chem 2004
:
Inhibition of ERK did not affect
interleukin-1beta induced
I-kappaBalpha phosphorylation and degradation but attenuated I-kappaBbeta degradation
Russo et al., Cell Signal 2004
:
Despite similar 125I-IL-1beta binding, HT-29 cells displayed no IRAK degradation, a 75 % reduction of IKK activity, and decreased
IkappaBalpha phosphorylation, NF-kappaB DNA binding activity and IL-8 mRNA accumulation in
response to
IL-1beta compared to Caco-2 cells
Kim et al., Biochem Pharmacol 2004
:
Epicatechin significantly reduced IL-1beta induced nitrite production, iNOS protein and mRNA expressions, and it also inhibited
IL-1beta induced
IkappaBalpha protein degradation, NF-kappaB activation, and iNOS promoter activity
Wang et al., Am J Physiol Lung Cell Mol Physiol 2005
:
As expected,
IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of
IkappaB-alpha were blocked by helenalin but not by U0126, SB-202190, or SP-600125
Panopoulos et al., J Biol Chem 2005
(Inflammation) :
Iron mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha induced but not
interleukin-1beta induced
IkappaB kinase complex/nuclear factor-kappaB activation
Ahmed et al., J Nutr 2005
:
PFE also inhibited the
IL-1beta induced phosphorylation of
IkappaBalpha and the DNA binding activity of the transcription factor NF-kappaB in OA chondrocytes
Stuhlmeier et al., J Biol Chem 2005
:
Stimulation of fibroblast-like synoviocytes with
IL-1beta resulted in rapid degradation of
IkappaBalpha , an event that was preceded by IkappaBalpha phosphorylation
Jalal et al., J Am Soc Nephrol 2006
:
Immunoprecipitation and immunoblotting with anti-IkappaBalpha and anti-phosphotyrosine antibodies revealed that PP2 also inhibited
IL-1beta stimulated tyrosine phosphorylation of
IkappaBalpha , a requisite step in NF-kappaB activation in this signaling cascade
Griffin et al., J Biol Chem 2006
(Astrocytoma...) :
However, in vitro analysis of enzyme activity demonstrates that
IL-1beta causes the long term activation of the
IkappaB kinase complex leading to chronic phosphorylation of the newly synthesized IkappaBalpha signal response domain and persistent activation of NFkappaB
Xu et al., Am J Physiol Cell Physiol 2006
(MAP Kinase Signaling System) :
In cultured rat VSMCs,
IL-1beta activated ERK and induced degradation of both
IkappaBalpha and IkappaBbeta, which was associated with nuclear translocation of both ribosomal S6 kinase (RSK)1 and NF-kappaB p65
Stuhlmeier et al., J Biol Chem 2007
(Rheumatic Diseases) :
Interestingly, AuTM does not interfere with
IL-1beta induced
IkappaB alpha degradation, in most cases a prerequisite for subsequent NFkappaB activation
Peng et al., Exp Cell Res 2007
(Inflammation...) :
We have further identified that interleukin-6 (IL-6) and
interleukin-1beta (IL-1beta) from PMN-melanoma co-cultures synergistically
contribute to
IkappaB-alpha degradation and IL-8 synthesis in PMNs. Taken together, these findings show that melanoma cells induce PMNs to secrete IL-8 through activation of NF-kappaB and suggest a model in which this interaction promotes a microenvironment that is favorable for metastasis
Tao et al., J Surg Res 2007
(Crohn Disease) :
VIP also decreased
IL-1beta induced NF-kappaB DNA binding capacity and phosphorylation of
IkappaB-alpha
Liang et al., J Cell Physiol 2007
(MAP Kinase Signaling System) :
Furthermore,
IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of
IkappaB-alpha was blocked by helenalin
Newton et al., J Pharmacol Exp Ther 2007
(Pulmonary Disease, Chronic Obstructive) :
In A549 cells, interleukin
(IL)-1beta and tumor necrosis factor (TNF) alpha
increased phosphorylation of
IkappaBalpha , and this was followed by loss of IkappaBalpha, induction of NF-kappaB DNA binding, and the induction of NF-kappaB dependent transcription
Tipton et al., J Periodontol 2007
(Periodontitis) :
IL-1beta increased
phospho-IkappaBalpha in whole cell extracts by a maximum of approximately 9.5 times ( P = 0.0001 ), and this was inhibited significantly by NS-398 ( P < or=0.008 )
Vidaeff et al., The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2007
(Premature Birth) :
Pretreatment with progesterone interfered with
IL-1beta induced
IkappaBalpha degradation
Nakao et al., Am J Pathol 2007
(Corneal Neovascularization) :
IL-1beta caused
IkappaB-alpha phosphorylation in corneal stromal cells but not in infiltrated CD11b ( + ) cells 2 days after IL-1beta implantation
Walsh et al., Shock 2008
:
IL-1beta causes nuclear accumulation of NF-kappaB ( Rel A ) but does not
increase nuclear
IkappaBalpha ... Furthermore, inhibition of exportin 1 attenuates
IL-1beta induced phosphorylation of
IkappaBalpha without affecting IkappaB kinase phosphorylation
Tsuji et al., Int Immunopharmacol 2007
:
IL-1beta induced phosphorylation of the mitogen activated protein ( MAP ) kinases,
IkappaBalpha , c-Jun and Akt
Romier et al., Br J Nutr 2008
(Colitis) :
Chrysin strongly reduced ( 2.5-fold )
IL-1beta induced
IkappaB-alpha phosphorylation, whereas ellagic acid increased it ( 1.7-fold )
Sun et al., J Steroid Biochem Mol Biol 2008
(Colonic Neoplasms) :
LCA pretreatment inhibited the
IL-1beta induced
IkappaBalpha degradation and decreased the NF-kappaB p65 phosphorylation
Shakibaei et al., Biochem Pharmacol 2008
(Osteoarthritis) :
Resveratrol, like N-Ac-Leu-Leu-norleucinal (ALLN) suppressed
IL-1beta induced proteasome function and the degradation of
IkappaBalpha ( an inhibitor of NF-kappaB ) without affecting IkappaBalpha kinase activation, IkappaBalpha-phosphorylation or IkappaBalpha-ubiquitination which suppressed nuclear translocation of the p65 subunit of NF-kappaB and its phosphorylation
Luo et al., Toxicol Appl Pharmacol 2008
:
Consistently,
IL-1beta stimulated both
IkappaB-alpha degradation and NF-kappaB translocation into nucleus in these cells
Kang et al., Biol Pharm Bull 2008
:
Troglitazone treatment of these cells also resulted in a dose dependent increase in
IL-1beta induced
IkappaBalpha phosphorylation
Choi et al., Scand J Gastroenterol 2008
(Adenocarcinoma...) :
Furthermore, LGG attenuated the IL-1beta induced transcriptional activation of the IL-8 gene and the NF-kappaB-responsive gene, and attenuated the
IL-1beta induced
IkappaBalpha degradation
Kimura et al., Invest Ophthalmol Vis Sci 2009
:
IL-1beta induced the phosphorylation and downregulation of
IkappaB-alpha as well as the translocation of p65 to the nucleus
Liao et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2009
:
The IKK-2 inhibitor, SC-514, inhibited
IL-1beta induced
IKappaBalpha protein activity, blocked p65 nuclear translocation, caused a significant reduction in IL-1beta induced DNA binding activity for p65 and ICAM-1 mRNA expression, and suppressed ICAM-1 expression on A549 cell surface ( all P < 0.01 )
Ahmad et al., J Immunol 2009
:
In addition,
IL-1beta induced phosphorylation of downstream effectors, IkappaB kinase alphabeta,
IkappaBalpha , and activation of transcription factor NF-kappaB was significantly reduced in the MyD88-, IRAK1-, TRAF6-, or Ras-deficient cells
Decker et al., Am J Physiol Cell Physiol 2009
(Astrocytoma...) :
LXA ( 4 ) ( 10 nM ) was found to inhibit
IL-1beta induced degradation of
IkappaBalpha ( P < 0.05 ), and the activation of an NFkappaB regulated reporter gene construct ( P < 0.05 )
Nam et al., PloS one 2009
(Inflammation) :
This suppression of NOS2 by DCS correlates with the attenuation of the NF-kappaB signaling pathway as measured by
IL-1beta induced phosphorylation of the
inhibitor of kappa B (IkappaB)-alpha , degradation of IkappaB-alpha and IkappaB-beta, and subsequent nuclear translocation of NF-kappaB p65
Joo et al., Eur J Pharmacol 2009
(Colitis) :
In HCT116 cells, the inhibitory effect by TauCl or 5-ASA was through preventing
IL-1beta induced
IkappaB kinase activation and subsequently interfering with IkappaBalpha degradation and p65 nuclear accumulation
Akanji et al., Inflamm Res 2010
:
Determine the
effect of
IL-1beta and dynamic compression on NFkappaB activation and
IkappaB-alpha gene expression in chondrocyte/agarose constructs ...
IL-1beta increased
IkappaB-alpha expression ( p < 0.001 ) at 60 min and either induced iNOS ( p < 0.001 ) and IL-1beta ( p < 0.01 ) or inhibited IL-4 ( p < 0.05 ) expression at 360 min
Xia et al., Glia 2010
:
IL-1beta stimulated
IkappaBalpha degradation, NF-kappaB nuclear translocation, and transactivation in astrocytes
Liu et al., Proc Natl Acad Sci U S A 2009
:
Neither JNK1 activation by
IL-1beta or UV nor TNF-alpha induced
activation of p38, ERK, or
IkappaB kinase complex is affected by the loss of Miz1
Kunze et al., Prostaglandins Other Lipid Mediat 2010
(Inflammation) :
C4S inhibited the enhanced expression of COX-2 and mPGES1 but had no effect on the
IL1beta induced decrease of
I-kappaBalpha and nuclear translocation of NF-kappaB
Mastrofrancesco et al., J Immunol 2010
(Melanoma, Experimental) :
Mechanistically, KdPT decreased
IL-1beta mediated
IkappaBalpha degradation, reduced nuclear accumulation of p65, and attenuated DNA binding of NF-kappaB
Ohtsuka et al., J Biol Chem 1996
(Melanoma) :
In addition, the degradation of
I kappa B-alpha induced by
IL-1 beta was not inhibited by dexamethasone
Jobin et al., J Immunol 1997
:
We demonstrate that
IL-1beta induces complete
IkappaB alpha degradation in Caco-2 cell lines but not in HT-29, T84, SW-480 transformed cell lines, or native colonic epithelial cells
Bonizzi et al., Eur J Biochem 1996
:
In these cells,
IL-1 beta rapidly
induces NF-kappa B through a complete degradation of
I kappa B-alpha , while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105
Bourcier et al., J Biol Chem 1997
(Arteriosclerosis) :
Interleukin-1beta stimulation
caused transient loss of
IkappaBalpha and a sustained decrease of IkappaBbeta that correlated with increased and persistent levels of p65/p50 protein and binding activity in the nucleus
Bergmann et al., J Biol Chem 1998
:
However,
IL-1beta induced
IkappaBalpha degradation as well as NF-kappaB nuclear translocation and DNA binding, as determined by Western blot and electro-mobility shift assay, respectively, are not affected by these inhibitors
Kwon et al., Diabetes 1998
:
TLCK and MG 132 inhibited both
IL-1beta induced activation of NFkappaB and degradation of
IkappaBalpha by islets and RINm5F cells
Lindroos et al., J Immunol 1998
:
However, while
IL-1beta and TNF-alpha both induced nuclear binding of the Rel proteins p50 and p65 to an NF-kappaB consensus oligonucleotide in gel shift assays and
caused transient degradation of inhibitor of NF-kappaB-alpha (
IkappaB-alpha ) in the cytoplasm of myofibroblasts, only IL-1beta upregulated PDGF-Ralpha
Katsuyama et al., Endocrinology 1998
(Translocation, Genetic) :
IL-1beta and TNF alpha, but not neutral SMase,
caused a transient decrease in
IkappaB-alpha protein levels, whereas IkappaB-beta protein expression was not affected by either agent