Gene interactions and pathways from curated databases and text-mining

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MED16 — MED7

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Lee et al., Mol Cell Biol 1999 : The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription of RNA polymerase II (PolII) and is composed of two stable subcomplexes ( Srb4 and Rgr1 subcomplexes )
Spåhr et al., J Biol Chem 2000 : This stimulation was species-specific, because S. pombe Mediator could not stimulate TFIIH purified from S. cerevisiae
Uehara et al., Cell Tissue Res 2001 : These immunocytochemical results suggest that PC2 may be involved in the proteolytic processing of SgII and that both PC1 and PC2 may be necessary to process CgA
Mittler et al., EMBO Rep 2001 : Novel critical role of a human Mediator complex for basal RNA polymerase II transcription
Wang et al., J Biol Chem 2002 : Mediator role of platelet derived growth factor and ERK
Delerive et al., Mol Endocrinol 2002 : By contrast, DRIP205 is not involved in PPARalpha mediated NF-kappaB transcriptional repression
Wang et al., J Virol 2002 (Cell Transformation, Viral) : Much of the large E1A bound to Mediator in 293 cells is in a stable complex that includes RNA polymerase II, leading us to suggest that the interaction of E1A-CR3 with Mediator stabilizes the interaction of Mediator with the polymerase
Acevedo et al., Mol Cell Biol 2003 : We have used a biochemical approach, including an in vitro chromatin assembly and transcription system, to examine the functional role for Mediator in the transcriptional activity of estrogen receptor alpha (ERalpha) with chromatin templates, as well as functional interplay between Mediator and p300/CBP during ERalpha dependent transcription ... Using three different approaches to functionally inactivate Mediator ( immunoneutralization, immunodepletion, and inhibitory polypeptides ), we find that Mediator is required for maximal transcriptional activation by ligand activated ERalpha
Miller et al., Neuropeptides 2003 : The absence of active PC2 resulted in altered cellular levels of NPY, somatostatin-28 , and ( Met ) enkephalin ; few changes in VIP or galanin occurred in the tissues examined ... The absence of active PC2 resulted in altered cellular levels of NPY , somatostatin-28, and ( Met ) enkephalin ; few changes in VIP or galanin occurred in the tissues examined
Zakharova et al., J Biol Chem 2003 : Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex ... Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex
Lee et al., Clin Exp Metastasis 2003 (MAP Kinase Signaling System...) : To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2 ... To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2 ... To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2
Marzban et al., Diabetes 2004 : Coexpression of proIAPP and PC2 resulted in production of mature IAPP , whereas in cells that coexpressed proIAPP and PC1/3 only a 6-kDa intermediate was produced
Kobayashi et al., Endocrine 2003 (Pituitary Neoplasms) : These results indicated that NaB and TGFbeta1 inhibit pituitary cell proliferation and regulate the expression of 7B2, PC1, and PC2 in a cell culture model of pituitary tumors ... These results indicated that NaB and TGFbeta1 inhibit pituitary cell proliferation and regulate the expression of 7B2, PC1, and PC2 in a cell culture model of pituitary tumors
Guidi et al., J Biol Chem 2004 : Mediator , a global transcriptional co-activator, dramatically enhances the phosphorylation of the CTD of RNA pol II by holo-TFIIH in vitro ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase
Sato et al., Mol Cell 2004 : The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors ... The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors
Pineda Torra et al., J Biol Chem 2004 : Requirement for the FXR/RXR heterodimer in the DRIP205-FXR interaction was evaluated using an RXR heterodimerization-deficient FXR mutant ( FXRL433R )
Richer et al., Proc Natl Acad Sci U S A 2004 : Our biochemical and kinetics analysis revealed that recombinant Spn4A inhibits human furin ( K ( i ), 13 pM ; k ( ass ), 3.2 x 10 ( 7 ) M ( -1 ) x s ( -1 ) ) and Drosophila PC2 ( K ( i ), 3.5 nM ; k ( ass ), 9.2 x 10 ( 4 ) M ( -1 ) x s ( -1 ) ) by a slow binding mechanism characteristic of serpin molecules and forms a kinetically trapped SDS-stable complex with each enzyme
Sanchez et al., J Clin Invest 2004 : Since this maturation in response to leptin requires prohormone processing, we hypothesized that leptin might regulate hypothalamic PC1 and PC2 expression, ultimately leading to coordinated processing of prohormones into mature peptides
Wang et al., Genetics 2004 : The rgr1 and sin4 constitutive phenotype does not require either the MAL-activator or maltose permease, indicating that Mediator represses MAL basal expression ... The role of the Mediator in MAL gene regulation is discussed
Pavri et al., Mol Cell 2005 : Importantly, Mediator was inactive ( Cdk8+ ) under basal conditions but was activated ( Cdk8- ) upon induction
Malik et al., Trends Biochem Sci 2005 : Dynamic regulation of pol II transcription by the mammalian Mediator complex
Govind et al., Mol Cell Biol 2005 : We confirm the roles of Mediator and SAGA in TATA binding protein (TBP) recruitment and demonstrate that all four coactivators under study enhance Pol II recruitment or promoter clearance following TBP binding
Li et al., J Biol Chem 2005 (Polycystic Kidney Diseases) : We employed a Xenopus oocyte Ca2+ imaging system to directly investigate the role of PC2 in inositol 1,4,5-trisphosphate ( IP3 ) -dependent Ca2+ signaling
Takagi et al., J Biol Chem 2006 : Taken together, these findings lead to the suggestion that Mediator is required for basal RNA polymerase II transcription in vivo
Kim et al., J Biol Chem 2006 : The beta-catenin transactivation domain bound directly to isolated MED12 and intact Mediator both in vitro and in vivo, and Mediator was recruited to Wnt-responsive genes in a beta-catenin dependent manner
Black et al., Mol Cell 2006 : Using purified proteins, we found that the Mediator regulates this assembly process by binding to p300 and TFIID
Zhou et al., Mol Cell Biol 2006 : We propose that activated Gli3 physically targets the MED12 interface within Mediator in order to functionally reverse Mediator dependent suppression of Shh target gene transcription
Wu et al., Hum Mol Genet 2006 : The FPC C-terminus substantially stimulated PC2 channel activity in the presence of KIF3B , whereas FPC or KIF3B alone had no effect
Arriagada et al., J Steroid Biochem Mol Biol 2007 : We find that both mutation of serine 208 to aspartic acid ( VDRS208D ) or phosphorylation of VDR by CKII enhance the interaction of VDR with DRIP205 in the presence of 1alpha,25-dihydroxy Vitamin D3
Fox et al., Frontiers in bioscience : a journal and virtual library 2007 : Evidence from our laboratory suggests that Nescient helix-loop-helix 2 (Nhlh2) regulates the transcription of PC1 and PC2 , possibly in conjunction with the leptin stimulated transcription factor, STAT3
Nillni et al., Endocrinology 2007 (Obesity) : Because biosynthesis of mature peptides in response to leptin requires prohormone processing, it is hypothesized that leptin might regulate hypothalamic PC1/3 and PC2 expression, ultimately leading to coordinated processing of prohormones into mature peptides
Liu et al., Mol Cell Biol 2008 : STAF65gamma is required for SPT3/STAGA interaction with core Mediator and for MYC recruitment of SPT3, TAF9, and core Mediator components to the TERT promoter but is dispensable for MYC recruitment of TRRAP, GCN5, and p300 and for acetylation of nucleosomes and loading of TFIID and RNA polymerase II on the promoter
Ge et al., Mol Cell Biol 2008 : These results indicate that there is a conditional requirement for MED1/TRAP220 and that a direct interaction between PPARgamma and Mediator through MED1/TRAP220 is not essential either for PPARgamma stimulated adipogenesis or for PPARgamma target gene expression in cultured fibroblasts ... As Mediator is apparently essential for PPARgamma transcriptional activity, our data indicate the presence of alternative mechanisms for Mediator recruitment, possibly through intermediate cofactors or other cofactors that are functionally redundant with MED1/TRAP220
Belakavadi et al., Mol Cell Biol 2008 : Here, we report that phosphorylation of MED1 by mitogen activated protein kinase-extracellular signal regulated kinase ( MAPK-ERK ) promotes its association with Mediator
Contreras-Levicoy et al., FEBS J 2008 : Activation of transcription by PC4 was dependent on the Mediator complex and TFIIA, but was independent of TATA binding protein associated factor
Tsutsui et al., Genes Cells 2008 : While the role of CDK8 has been studied extensively, little is known of the role of CDK11 in Mediator
Celić et al., J Biol Chem 2008 : Our results suggest that PC2-CC is involved in PC2 oligomerization, and PC2-EF is a Ca2+-sensitive switch
Thiaville et al., Nucleic Acids Res 2008 : It is unclear whether Mediator complex in yeast is necessary for all RNA polymerase II (Pol II) transcription or if it is limited to genes activated by environmental stress
Geng et al., Proc Natl Acad Sci U S A 2008 (Calcium Signaling) : Single channel studies showed that interaction with Stx5 specifically reduces PC2 channel activity
Katz et al., Mol Cell Biol 2009 : By binding and transactivation studies, we found that Pax6 indirectly regulates PC2 gene transcription through cMaf and Beta2/NeuroD1 while it activates the 7B2 gene both directly and indirectly through the same transcription factors, cMaf and Beta2/NeuroD1
Wezensky et al., Gene 2010 : Co-immunoprecipitation and promoter-reporter studies reveal that the effect of PC2 on PLAGL2 target promoter activity was conferred via the C-terminus of PLAGL2, the region that is required for PC2 binding and contains the PLAGL2 activation domain
Vincourt et al., Cancer Res 2010 (Bone Neoplasms...) : In contrast, soluble PC1CP, but not PC2CP , induced the migration of EAhy926 cells and increased vascular endothelial growth factor ( VEGF ) and CXCR4 expression in chondrocytes ... In contrast, soluble PC1CP, but not PC2CP , induced the migration of EAhy926 cells and increased vascular endothelial growth factor ( VEGF ) and CXCR4 expression in chondrocytes ... Soluble PC2CP also increased VEGF expression, but along with a more pronounced effect on CXCR4 and matrix metalloproteinase 13 expression
Carey et al., Cold Spring Harbor protocols 2010 : INTRODUCTION : The Mediator ( Med ) complex plays a key role in promoter-specific activation of transcription by RNA polymerase II (Pol II)
Kunin et al., PloS one 2010 : Mediator of DNA damage checkpoint 1 ( MDC1 ) contributes to high NaCl induced activation of the osmoprotective transcription factor TonEBP/OREBP
Xu et al., EMBO Rep 2011 (Alzheimer Disease) : Mediator , in a MED12 dependent manner, occupies only AICD bound promoter DNA, indicating that the AICD recruits Mediator to activate transcription
Lin et al., Genes Dev 2011 : Studies in depleted extracts showed that the Mediator coactivator complex, which controls PIC assembly, is also necessary for CHD1 recruitment
Ang et al., PLoS Biol 2012 : Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80 , which places Mediator genetically upstream of the activator Gal4 ... Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80, which places Mediator genetically upstream of the activator Gal4
Yu et al., Inflammation 2012 : Removal of Ca ( 2+ ) by Ca ( 2+ ) chelator BAPTA or inhibition of protein kinase A (PKA) by the PKA inhibitors H-89 each partially reduced PC(2)s induced mucin secretion
Lewis et al., Clin Exp Allergy 2013 : Mediator release induced by SCF was accompanied by the up-regulation of the activation marker, CD63
Kim et al., J Biol Chem 2013 : Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16 ... Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16
Verger et al., Nucleic Acids Res 2013 : We further show that depletion of MED25 disrupts the association of ERM with the Mediator in vitro
Galbraith et al., Cell 2013 (Neoplasms) : HIF1A Employs CDK8-Mediator to Stimulate RNAPII Elongation in Response to Hypoxia ... HIF1A induces binding of CDK8-Mediator and the super elongation complex ( SEC ), containing AFF4 and CDK9, to alleviate RNAPII pausing
Šedý et al., J Immunol 2013 (Inflammation) : CD160 Activation by Herpesvirus Entry Mediator Augments Inflammatory Cytokine Production and Cytolytic Function by NK Cells
Newman et al., Endocrinology 1985 : Mediator generation was rapid, with a half-time of approximately 45 sec and was insulin dose dependent
Day et al., DNA Cell Biol 1995 : Maintained PC1 and PC2 expression in the AtT-20 variant cell line 6T3 lacking regulated secretion and POMC : restored POMC expression and regulated secretion after cAMP treatment
Jansen et al., Biochem J 1997 (Insulinoma) : Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity
Johanning et al., J Biol Chem 1998 : Analysis of proenkephalin reaction products using immunoblotting and gel permeation chromatography demonstrated that PC2 can directly cleave proenkephalin and that the generation of small opioid peptides from intermediates is mediated almost entirely by PC2 rather than by PC1 ... Analysis of proenkephalin reaction products using immunoblotting and gel permeation chromatography demonstrated that PC2 can directly cleave proenkephalin and that the generation of small opioid peptides from intermediates is mediated almost entirely by PC2 rather than by PC1
Han et al., Mol Cell Biol 1999 : The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription