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IQGAP1 — RAC1
Pathways - manually collected, often from reviews:
-
KEGG Adherens junction:
RAC1/RAC2/RAC3
→
IQGAP1
(protein-protein, activation)
-
KEGG Adherens junction:
CDC42/RAC1/RAC2/RAC3
→
IQGAP1
(protein-protein, inhibition)
-
KEGG Regulation of actin cytoskeleton:
RAC1/RAC2/RAC3
→
IQGAP1/IQGAP2/IQGAP3
(protein-protein, activation)
-
NCI Pathway Database RAC1 signaling pathway:
E-cadherin/Ca2+/beta catenin/alpha catenin complex (CDH1-CTNNB1-CTNNA1)
→
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Kuroda et al., Science 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
→
None
(modification, inhibits)
Fukata et al., J Biol Chem 1999, Kuroda et al., Science 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
→
IQGAP1 (IQGAP1)
(modification, inhibits)
Fukata et al., J Biol Chem 1999, Kuroda et al., Science 1998
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
IQGAP1 (IQGAP1)
→
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Nakagawa et al., J Cell Sci 2001, Kuroda et al., Science 1998
Evidence: physical interaction
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
IQGAP1 (IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Nakagawa et al., J Cell Sci 2001, Kuroda et al., Science 1998
Evidence: physical interaction
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Nakagawa et al., J Cell Sci 2001, Kuroda et al., Science 1998
Evidence: physical interaction
-
NCI Pathway Database E-cadherin signaling in the nascent adherens junction:
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
→
CDC42/GTP/IQGAP1 complex (CDC42-IQGAP1)
(translocation, inhibits)
Le et al., J Cell Biol 1999*, Izumi et al., J Cell Biol 2004*
Evidence: mutant phenotype, assay
-
NCI Pathway Database Nectin adhesion pathway:
RAC1/GTP/IQGAP1/filamentous actin complex (RAC1-IQGAP1)
→
IQGAP1 (IQGAP1)
(modification, collaborate)
Izumi et al., J Cell Biol 2004*
Evidence: mutant phenotype
-
NCI Pathway Database Nectin adhesion pathway:
RAC1/GTP/IQGAP1/filamentous actin complex (RAC1-IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Izumi et al., J Cell Biol 2004*
Evidence: mutant phenotype
-
NCI Pathway Database Nectin adhesion pathway:
IQGAP1 (IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Izumi et al., J Cell Biol 2004*
Evidence: mutant phenotype
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
IQGAP1 (IQGAP1)
→
RAC1-CDC42/GTP/IQGAP1 complex (RAC1_CDC42-IQGAP1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Kuroda et al., Science 1998
Evidence: physical interaction
-
NCI Pathway Database Posttranslational regulation of adherens junction stability and dissassembly:
E-cadherin/Ca2+/beta catenin/alpha catenin/p120 catenin complex (CDH1-CTNNB1-CTNNA1-CTNND1)
→
RAC1-CDC42/GTP/IQGAP1 complex (RAC1_CDC42-IQGAP1)
(modification, collaborate)
Fukata et al., J Biol Chem 1999, Kuroda et al., Science 1998
Evidence: physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
IQGAP1 (IQGAP1)
→
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
(modification, collaborate)
Kuroda et al., J Biol Chem 1996*
Evidence: physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
IQGAP1 (IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Kuroda et al., J Biol Chem 1996*
Evidence: physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
RAC1/GTP/IQGAP1 complex (RAC1-IQGAP1)
→
RAC1/GTP complex (RAC1)
(modification, collaborate)
Kuroda et al., J Biol Chem 1996*
Evidence: physical interaction
-
WikiPathways Regulation of Actin Cytoskeleton:
RAC1/RAC3/RAC2
→
IQGAP1
(activation)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
IQGAP1
—
RAC1
(direct interaction, pull down)
Kuroda et al., J Biol Chem 1996*
-
IRef Biogrid Interaction:
IQGAP1
—
RAC1
(physical association, affinity chromatography technology)
Fukata et al., Cell 2002*
-
IRef Biogrid Interaction:
IQGAP1
—
RAC1
(physical association, affinity chromatography technology)
Hart et al., EMBO J 1996*
-
IRef Biogrid Interaction:
IQGAP1
—
RAC1
(direct interaction, pull down)
Zhang et al., J Biol Chem 1998
-
IRef Corum Interaction:
Complex of APC-IQGAP1-RAC1
(association, anti bait coimmunoprecipitation)
Watanabe et al., Dev Cell 2004*
-
IRef Hprd Interaction:
IQGAP1
—
RAC1
(in vitro)
Yan et al., Oncogene 2009*, Hart et al., EMBO J 1996*, McCallum et al., J Biol Chem 1996*, Brill et al., Mol Cell Biol 1996*, Kuroda et al., J Biol Chem 1996*
-
IRef Hprd Interaction:
IQGAP1
—
RAC1
(in vivo)
Yan et al., Oncogene 2009*, Hart et al., EMBO J 1996*, McCallum et al., J Biol Chem 1996*, Brill et al., Mol Cell Biol 1996*, Kuroda et al., J Biol Chem 1996*
-
IRef Intact Interaction:
Complex of 23 proteins
(association, tandem affinity purification)
Villacé et al., Nucleic Acids Res 2004
-
IRef Intact Interaction:
Complex of 185 proteins
(association, cross-linking study)
Humphries et al., Science signaling 2009
-
IRef Intact Interaction:
Complex of 177 proteins
(association, cross-linking study)
Byron et al., Proteomics 2012
-
IRef Intact Interaction:
Complex of 400 proteins
(association, cross-linking study)
Humphries et al., Science signaling 2009
-
IRef Intact Interaction:
Complex of 203 proteins
(association, cross-linking study)
Byron et al., Proteomics 2012
-
IRef Ophid Interaction:
RAC1
—
IQGAP1
(aggregation, confirmational text mining)
Hart et al., EMBO J 1996*
-
IRef Ophid Interaction:
RAC1
—
IQGAP1
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Fukata et al., J Biol Chem 1999
:
Cdc42 and
Rac1 regulate the interaction of
IQGAP1 with beta-catenin ... These results indicate that Cdc42 and
Rac1 negatively
regulate the IQGAP1 function by inhibiting the interaction of
IQGAP1 with beta-catenin, leading to stabilization of the cadherin-catenin complex
Fukata et al., J Cell Sci 1999
:
Activated Cdc42 and
Rac1 inhibit
IQGAP1 , thereby stabilizing the cadherin-catenins complex
Fukata et al., Mol Cell Biol 2001
:
We have previously proposed that
IQGAP1 , an effector of Rac1 and Cdc42, negatively regulates cadherin mediated cell-cell adhesion by interacting with beta-catenin and by causing the dissociation of alpha-catenin from cadherin-beta-catenin-alpha-catenin complexes and that activated
Rac1 and Cdc42 positively
regulate cadherin mediated cell-cell adhesion by inhibiting the interaction of IQGAP1 with beta-catenin
Noritake et al., Mol Biol Cell 2004
:
Together, these results indicate that Rac1 enhances the accumulation of actin filaments, E-cadherin, and beta-catenin by acting on IQGAP1 and suggest that there exists a positive feedback loop comprised of "E-cadherin mediated cell-cell adhesion -- >
Rac1 activation -- > actin-meshwork formation by
IQGAP1 -- > increasing E-cadherin mediated cell-cell adhesion. ''
Takahashi et al., J Cell Physiol 2006
:
While a small GTPase Rac and its effector
IQGAP1 were associated with the E-cadherin-catenins complex, either OA or PP2A-A siRNA concomitantly
induced the dissociation of IQGAP1, but not
Rac , from the complex and the internalization of E-cadherin from the cell surface
Watanabe et al., Novartis Found Symp 2005
(Adenomatous Polyposis Coli) :
Activated
Rac1 and Cdc42
enhance the binding of
IQGAP1 to CLIP-170, and capture GFP-CLIP-170 at the base of leading edges and filopodia, respectively
Brown et al., J Biol Chem 2007
(Salmonella Infections) :
IQGAP1 regulates Salmonella invasion through interactions with actin,
Rac1 , and Cdc42 ... Overexpression of
IQGAP1 significantly increases the ability of Salmonella to enter host cells and
required interaction with both actin and
Cdc42/Rac1
Usatyuk et al., J Biol Chem 2009
(Hyperoxia) :
Phospholipase D-mediated
activation of
IQGAP1 through
Rac1 regulates hyperoxia induced p47phox translocation and reactive oxygen species generation in lung endothelial cells ... Hyperoxia caused rapid activation and redistribution of Rac1, and IQGAP1 to cell periphery, and down-regulation of
Rac1 , and
IQGAP1 attenuated hyperoxia induced tyrosine phosphorylation of Src and cortactin and ROS generation
Kozlova et al., Cell Signal 2012
:
Depletion of
IQGAP1 suppressed hyaluronan induced activation of
Rac1 and enhanced the activation of RhoA
Kobayashi et al., J Biol Chem 1998
:
We have previously identified
IQGAP1 from bovine brain cytosol as a
target for
Rac1 by an affinity purification method