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NCK1 — WASL

Pathways - manually collected, often from reviews:

• NCI Pathway Database Nephrin/Neph1 signaling in the kidney podocyte: N-WASP (WASL) → Nephrin/NEPH1/podocin/NCK1-2 complex (NPHS1-KIRREL-NPHS2-NCK2_NCK1) (modification, collaborate)
  Blasutig et al., Mol Cell Biol 2008
  Evidence: mutant phenotype
• NCI Pathway Database Nephrin/Neph1 signaling in the kidney podocyte: N-WASP (WASL) → Nephrin/NEPH1/podocin/NCK1-2/N-WASP complex (NPHS1-KIRREL-NPHS2-NCK2_NCK1-WASL) (modification, collaborate)
  Blasutig et al., Mol Cell Biol 2008
  Evidence: mutant phenotype
• NCI Pathway Database Nephrin/Neph1 signaling in the kidney podocyte: Nephrin/NEPH1/podocin/NCK1-2 complex (NPHS1-KIRREL-NPHS2-NCK2_NCK1) → Nephrin/NEPH1/podocin/NCK1-2/N-WASP complex (NPHS1-KIRREL-NPHS2-NCK2_NCK1-WASL) (modification, collaborate)
  Blasutig et al., Mol Cell Biol 2008
  Evidence: mutant phenotype
• NCI Pathway Database PDGFR-beta signaling pathway: RAC1/GTP complex (RAC1) → PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP/NCK1-2 complex (PDGFRB-GAB1-WASL-NCK2_NCK1-PDGFB) (modification, activates)
  Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, assay
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): HGF(dimer)/MET(dimer)/GAB1/N-WASP/NCK1-2 complex (MET-GAB1-WASL-NCK2_NCK1-HGF) → HGF(dimer)/MET(dimer)/GAB1/N-WASP complex (MET-GAB1-WASL-HGF) (modification, collaborate)
  Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): HGF(dimer)/MET(dimer)/GAB1/N-WASP/NCK1-2 complex (MET-GAB1-WASL-NCK2_NCK1-HGF) → NCK1-2 (NCK2/NCK1) (modification, collaborate) Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): HGF(dimer)/MET(dimer)/GAB1/N-WASP complex (MET-GAB1-WASL-HGF) → NCK1-2 (NCK2/NCK1) (modification, collaborate) Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP complex (PDGFRB-GAB1-WASL-PDGFB) → NCK1-2 (NCK2/NCK1) (modification, collaborate) Kallin et al., J Biol Chem 2004, Ruusala et al., J Biol Chem 2008, Abella et al., J Cell Sci 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP complex (PDGFRB-GAB1-WASL-PDGFB) → PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP/NCK1-2 complex (PDGFRB-GAB1-WASL-NCK2_NCK1-PDGFB) (modification, collaborate)
  Kallin et al., J Biol Chem 2004, Ruusala et al., J Biol Chem 2008, Abella et al., J Cell Sci 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: NCK1-2 (NCK2/NCK1) → PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP/NCK1-2 complex (PDGFRB-GAB1-WASL-NCK2_NCK1-PDGFB) (modification, collaborate)
  Kallin et al., J Biol Chem 2004, Ruusala et al., J Biol Chem 2008, Abella et al., J Cell Sci 2010
  Evidence: physical interaction
• NCI Pathway Database PDGFR-beta signaling pathway: None → PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP/NCK1-2 complex (PDGFRB-GAB1-WASL-NCK2_NCK1-PDGFB) (modification, collaborate)
  Kallin et al., J Biol Chem 2004, Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, assay
• NCI Pathway Database PDGFR-beta signaling pathway: RAC1/GDP complex (RAC1) → PDGF-BB/PDGFRB (dimer)/GAB1/N-WASP/NCK1-2 complex (PDGFRB-GAB1-WASL-NCK2_NCK1-PDGFB) (modification, collaborate)
  Kallin et al., J Biol Chem 2004, Abella et al., J Cell Sci 2010
  Evidence: mutant phenotype, assay
• Reactome Reaction: NCK1 → WASL (indirect_complex) Rohatgi et al., J Cell Biol 2000*, Rohatgi et al., J Biol Chem 2001, Millard et al., Biochem J 2004*, Jones et al., Nature 2006
• Reactome Reaction: NCK1 → WASL (direct_complex) Carlier et al., J Biol Chem 2000, Moreau et al., Nat Cell Biol 2000, Fukuoka et al., J Cell Biol 2001, Rohatgi et al., J Biol Chem 2001, Ho et al., Proc Natl Acad Sci U S A 2001, Kato et al., Biochem Biophys Res Commun 2002, Aspenström et al., Exp Cell Res 2002, Cory et al., J Biol Chem 2002, Wu et al., J Biol Chem 2004
• Reactome Reaction: NCK1 → WASL (reaction) Carlier et al., J Biol Chem 2000, Moreau et al., Nat Cell Biol 2000, Rohatgi et al., J Cell Biol 2000*, Fukuoka et al., J Cell Biol 2001, Rohatgi et al., J Biol Chem 2001, Ho et al., Proc Natl Acad Sci U S A 2001, Kato et al., Biochem Biophys Res Commun 2002, Aspenström et al., Exp Cell Res 2002, Millard et al., Biochem J 2004*, Jones et al., Nature 2006

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: WASL — NCK1 (direct interaction, far western blotting) Rohatgi et al., J Biol Chem 2001
• IRef Biogrid Interaction: WASL — NCK1 (direct interaction, pull down) Rohatgi et al., J Biol Chem 2001
• IRef Hprd Interaction: NCK1 — WASL (in vitro) Rohatgi et al., J Biol Chem 2001
• IRef Intact Interaction: WASL — NCK1 (physical association, peptide array) Wu et al., Proteomics 2007
• IRef Ophid Interaction: WASL — NCK1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Tomasevic et al., Biochemistry 2007 : Differential regulation of WASP and N-WASP by Cdc42, Rac1, Nck , and PI ( 4,5 ) P2
Girard et al., Infect Immun 2009 (Disease Models, Animal...) : Strains belonging to the EPEC-1 lineage trigger strong actin polymerization via tyrosine phosphorylation of the type III secretion system ( T3SS ) effector Tir, recruitment of Nck , and activation of N-WASP
Abella et al., J Cell Sci 2010 : Formation of dorsal ruffles requires interaction between Gab1 and Nck , and also requires functional N-WASP
Donnelly et al., Curr Biol 2013 : N-WASP also contains two Nck binding sites, but its recruitment is dependent on its interaction with WIP rather than Nck