Gene interactions and pathways from curated databases and text-mining

◀ Back to MED16

MED16 — MED24

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Lee et al., Mol Cell Biol 1999 : The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription of RNA polymerase II (PolII) and is composed of two stable subcomplexes ( Srb4 and Rgr1 subcomplexes )
Strukova et al., Biochemistry (Mosc) 1999 (Inflammation) : TRAP-6 inhibited PAF release from A23187 activated RPMC via an NO-dependent mechanism
Hoshi et al., J Biol Chem 2000 : NF-kappaB was activated after the stimulation of TRAP, bFGF, and TNF-alpha in electrophoretic mobility shift assay, and E5510 suppressed the NF-kappaB activation induced by TRAP and bFGF but not the activation by TNF-alpha ... NF-kappaB was activated after the stimulation of TRAP, bFGF, and TNF-alpha in electrophoretic mobility shift assay, and E5510 suppressed the NF-kappaB activation induced by TRAP and bFGF but not the activation by TNF-alpha ... The phosphorylation of ERK1/2 was rapidly induced by TRAP and bFGF but not by TNF-alpha ... These results indicate that TRAP and bFGF induced I-kappaB degradation and NF-kappaB activation through a distinct pathway from TNF-alpha and that ERK1/2 may play an important role in NF-kappaB activation induced by TRAP and bFGF
Spåhr et al., J Biol Chem 2000 : This stimulation was species-specific, because S. pombe Mediator could not stimulate TFIIH purified from S. cerevisiae
Ohmori et al., Biochemistry 2000 : While BAPTA-AM ( an intracellular Ca2+ chelator ) inhibited Cas phosphorylation induced by collagen or TRAP , Ro31-8220 ( a PKC inhibitor ) rather prolonged it
Uehara et al., Cell Tissue Res 2001 : These immunocytochemical results suggest that PC2 may be involved in the proteolytic processing of SgII and that both PC1 and PC2 may be necessary to process CgA
Kimura et al., Planta Med 2001 : Intercellular adhesion molecule-1 ( ICAM-1 ) expression was also induced by thrombin, but not by TRAP
Weber et al., Blood 2001 : ADP induced fibrinogen binding was completely inhibited by 10 microg/mL abciximab, 30 nM tirofiban, or 3 microg/mL eptifibatide, while fibrinogen binding induced by 100 microM TRAP was inhibited only by 50 %
de Rossi et al., Anesth Analg 2001 : In contrast, halothane reduced TRAP-6 induced activation of the GPIIb/IIIa complex
Mittler et al., EMBO Rep 2001 : Novel critical role of a human Mediator complex for basal RNA polymerase II transcription
Huang et al., Zhonghua Xue Ye Xue Za Zhi 1999 : The expression of GPIb was reduced while the expressions of GPIIb/IIIa , IIIa, P-selectin increased after the platelets were activated by TRAP
Sakai et al., J Biochem 2001 : Receptor activator of NF-kappaB ( RANKL ) significantly increased the LBC levels of cathepsin K, TRAP , and c-Src, whereas calcitonin decreased the LBC levels of cathepsin K, TRAP, and Rab7, indicating that the transformation of early to late endosomal elements and lysosomes in OCLs is also regulated by osteoclastogenesis regulatory factors
Espinola et al., Thromb Haemost 2002 : TRAP increased the expression of GPIIb/IIIa and GPIIIa on platelet surface ... TRAP increased the expression of GPIIb/IIIa and GPIIIa on platelet surface
Wang et al., J Biol Chem 2002 : Mediator role of platelet derived growth factor and ERK
Delerive et al., Mol Endocrinol 2002 : By contrast, DRIP205 is not involved in PPARalpha mediated NF-kappaB transcriptional repression
Wang et al., J Virol 2002 (Cell Transformation, Viral) : Much of the large E1A bound to Mediator in 293 cells is in a stable complex that includes RNA polymerase II, leading us to suggest that the interaction of E1A-CR3 with Mediator stabilizes the interaction of Mediator with the polymerase
Shui et al., Calcif Tissue Int 2002 : The combination of rapamycin ( 10 ng/ml ) and TGF-beta1 ( 1 ng/ml ) increased TRAP+MNC 3.1- and 6.9-fold as compared with rapamycin or TGF-beta1 alone, respectively, and enhanced CTR mRNA expression induced by TGF-beta1 by 1.9-fold
Wang et al., J Biol Chem 2002 (Prostatic Neoplasms) : A coregulatory role for the TRAP-mediator complex in androgen receptor mediated gene expression
Sheu et al., J Biol Chem 2003 : Moreover, we show that TRAP interacts with TRIP intracellularly, that activation of the TGFbeta type II receptor by TRIP-1 occurs in the presence of TRAP and that the differentiation process is mediated through the Smad2/3 pathway ... Moreover, we show that TRAP interacts with TRIP intracellularly, that activation of the TGFbeta type II receptor by TRIP-1 occurs in the presence of TRAP and that the differentiation process is mediated through the Smad2/3 pathway
Acevedo et al., Mol Cell Biol 2003 : We have used a biochemical approach, including an in vitro chromatin assembly and transcription system, to examine the functional role for Mediator in the transcriptional activity of estrogen receptor alpha (ERalpha) with chromatin templates, as well as functional interplay between Mediator and p300/CBP during ERalpha dependent transcription ... Using three different approaches to functionally inactivate Mediator ( immunoneutralization, immunodepletion, and inhibitory polypeptides ), we find that Mediator is required for maximal transcriptional activation by ligand activated ERalpha
Billheimer et al., Thromb Res 2002 (Thrombosis) : All four fibans inhibited ADP and TRAP stimulated fibrinogen binding and microaggregate formation in a concentration dependent manner, whereas P-selectin expression was relatively unaltered
Liu et al., J Biol Chem 2003 : Moreover, the binding is significantly enhanced in EMSA with nuclear extracts from RANKL treated RAW264.7 cells and BMMs, suggesting that the 12-bp sequence may be involved in RANKL induced TRAP transcription ... Importantly, mutation of the USF binding site partially blocks RANKL induced TRAP transcription in RAW264.7 cells, confirming that USF1 and USF2 are functionally involved in RANKL induced TRAP transcription ... Importantly, mutation of the USF binding site partially blocks RANKL induced TRAP transcription in RAW264.7 cells, confirming that USF1 and USF2 are functionally involved in RANKL induced TRAP transcription ... Importantly, mutation of the USF binding site partially blocks RANKL induced TRAP transcription in RAW264.7 cells, confirming that USF1 and USF2 are functionally involved in RANKL induced TRAP transcription
Miller et al., Neuropeptides 2003 : The absence of active PC2 resulted in altered cellular levels of NPY, somatostatin-28 , and ( Met ) enkephalin ; few changes in VIP or galanin occurred in the tissues examined ... The absence of active PC2 resulted in altered cellular levels of NPY , somatostatin-28, and ( Met ) enkephalin ; few changes in VIP or galanin occurred in the tissues examined
Zakharova et al., J Biol Chem 2003 : Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex ... Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex ... Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex ... Furthermore, in a system with purified proteins and naked DNA, STAT1alpha- and STAT1beta dependent transcription is stimulated by the TRAP/Mediator co-activator complex
Reiter et al., Thromb Haemost 2003 (Endotoxemia...) : PAR1 decreased by approximately 8 % ( p < 0.001 ) within 2 h after endotoxin infusion and stayed at those levels until 6 h. Concomitantly, TRAP induced P-selectin expression maximally decreased by 18 % ( p < 0.001 ) at 6 h ... PAR1 decreased by approximately 8 % ( p < 0.001 ) within 2 h after endotoxin infusion and stayed at those levels until 6 h. Concomitantly, TRAP induced P-selectin expression maximally decreased by 18 % ( p < 0.001 ) at 6 h
Lee et al., Clin Exp Metastasis 2003 (MAP Kinase Signaling System...) : To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2 ... To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2 ... To investigate the role of HGF/c-met signaling on metastasis in cancer cells stimulated with HGF, we examined the effects of a specific MEK1 inhibitor ( PD98059 ) and a p38 MAP kinase inhibitor ( SB203580 ) on HGF induced uPA expression in pancreatic cancer cell lines, L3.6PL and IMIM-PC2
Marzban et al., Diabetes 2004 : Coexpression of proIAPP and PC2 resulted in production of mature IAPP , whereas in cells that coexpressed proIAPP and PC1/3 only a 6-kDa intermediate was produced
Kobayashi et al., Endocrine 2003 (Pituitary Neoplasms) : These results indicated that NaB and TGFbeta1 inhibit pituitary cell proliferation and regulate the expression of 7B2, PC1, and PC2 in a cell culture model of pituitary tumors ... These results indicated that NaB and TGFbeta1 inhibit pituitary cell proliferation and regulate the expression of 7B2, PC1, and PC2 in a cell culture model of pituitary tumors
Guidi et al., J Biol Chem 2004 : Mediator , a global transcriptional co-activator, dramatically enhances the phosphorylation of the CTD of RNA pol II by holo-TFIIH in vitro ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase ... Using purified proteins we have determined that the subunits of TFIIK are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphorylation of a glutathione S-transferase-CTD fusion protein, despite the absence of multiple Mediator and/or TFIIH interactions with polymerase
Sato et al., Mol Cell 2004 : The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors ... The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors
Pineda Torra et al., J Biol Chem 2004 : Requirement for the FXR/RXR heterodimer in the DRIP205-FXR interaction was evaluated using an RXR heterodimerization-deficient FXR mutant ( FXRL433R )
Richer et al., Proc Natl Acad Sci U S A 2004 : Our biochemical and kinetics analysis revealed that recombinant Spn4A inhibits human furin ( K ( i ), 13 pM ; k ( ass ), 3.2 x 10 ( 7 ) M ( -1 ) x s ( -1 ) ) and Drosophila PC2 ( K ( i ), 3.5 nM ; k ( ass ), 9.2 x 10 ( 4 ) M ( -1 ) x s ( -1 ) ) by a slow binding mechanism characteristic of serpin molecules and forms a kinetically trapped SDS-stable complex with each enzyme
Sanchez et al., J Clin Invest 2004 : Since this maturation in response to leptin requires prohormone processing, we hypothesized that leptin might regulate hypothalamic PC1 and PC2 expression, ultimately leading to coordinated processing of prohormones into mature peptides
Wang et al., Genetics 2004 : The rgr1 and sin4 constitutive phenotype does not require either the MAL-activator or maltose permease, indicating that Mediator represses MAL basal expression ... The role of the Mediator in MAL gene regulation is discussed
Shi et al., Gene 2004 : Functionally, mutation of the YY1 binding site resulted in a reduction in the RANKL induced TRAP transcription in RAW264.7 cells, demonstrating that YY1 positively regulates RANKL induced TRAP transcriptional activation ... Functionally, mutation of the YY1 binding site resulted in a reduction in the RANKL induced TRAP transcription in RAW264.7 cells, demonstrating that YY1 positively regulates RANKL induced TRAP transcriptional activation
Fukushima et al., Bone 2005 : The addition of cell-permeable PKI, an inhibitor of the cAMP/PKA signaling pathway, to the cocultures 8 h after the IL-1alpha stimulation inhibited IL-1alpha induced TRAP+ cell formation ... IL-1alpha induced TRAP+ cell formation was completely blocked by either NS398, a selective inhibitor of cyclooxygenase (COX)-2 , or PD98059, a specific inhibitor of extracellular signal regulated kinase ( ERK ) ... IL-1alpha induced TRAP+ cell formation was completely blocked by either NS398, a selective inhibitor of cyclooxygenase (COX)-2, or PD98059, a specific inhibitor of extracellular signal regulated kinase ( ERK ) ... IL-1alpha induced TRAP+ cell formation was completely blocked by either NS398, a selective inhibitor of cyclooxygenase (COX)-2 , or PD98059, a specific inhibitor of extracellular signal regulated kinase ( ERK )
Fukushima et al., J Dent Res 2005 : The PKC inhibitor, Ro-32-0432, suppressed RANKL expression in PDL cells and PTHrP induced TRAP+ cell formation
Pavri et al., Mol Cell 2005 : Importantly, Mediator was inactive ( Cdk8+ ) under basal conditions but was activated ( Cdk8- ) upon induction
Malik et al., Trends Biochem Sci 2005 : Dynamic regulation of pol II transcription by the mammalian Mediator complex
Ljusberg et al., J Biol Chem 2005 : Activation of TRAP by cathepsin K/L was because of increases in catalytic activity, substrate affinity, and sensitivity to reductants ... Activation of TRAP by cathepsin K/L was because of increases in catalytic activity, substrate affinity, and sensitivity to reductants
Govind et al., Mol Cell Biol 2005 : We confirm the roles of Mediator and SAGA in TATA binding protein (TBP) recruitment and demonstrate that all four coactivators under study enhance Pol II recruitment or promoter clearance following TBP binding
Stephenson et al., The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2005 (Fetal Membranes, Premature Rupture) : TRAP-14 , a thrombin receptor agonist, also significantly increased MMP-9 levels, suggesting that thrombin induced changes in MMP-9 expression were mediated through the thrombin receptor
Soulet et al., Journal of thrombosis and haemostasis : JTH 2005 : Conversely, secreted ADP strongly potentiated Rac activation induced by FcgammaRIIa clustering or TRAP via its P2Y12 receptor, the target of antithrombotic thienopyridines
Li et al., J Biol Chem 2005 (Polycystic Kidney Diseases) : We employed a Xenopus oocyte Ca2+ imaging system to directly investigate the role of PC2 in inositol 1,4,5-trisphosphate ( IP3 ) -dependent Ca2+ signaling
Takagi et al., J Biol Chem 2006 : Taken together, these findings lead to the suggestion that Mediator is required for basal RNA polymerase II transcription in vivo
Kim et al., J Biol Chem 2006 (Bone Resorption) : We propose that the MCP-1 induced TRAP ( + ) /CTR ( + ) multinuclear cells represent an arrested stage in osteoclast differentiation, after NFATc1 induction and cellular fusion but prior to the development of bone resorption activity
Sarker et al., Pathophysiol Haemost Thromb 2005 (Calcium Signaling...) : Previously, we showed that thrombin and the thrombin receptor agonist peptide ( TRAP-14 ; SFLLRNPNDKYEPF ) for protease activated receptor 1 (PAR1) induce vascular endothelial growth factor ( VEGF ) secretion in PC-12 cells ... In this study, we show that thrombin and TRAP-14 also stimulate VEGF secretion in the human NB-1 neuroblastoma cells
Schneider et al., Can J Appl Physiol 2005 : In plasma, LPO decreased from 3589 +/- 193 to 3274 +/- 223 cps x mg Hb ( -1 ) ( p < 0.05 ), and TRAP increased from 304 +/- 45 to 384 +/- 57 micromol x L ( -1 ) trolox ( p < 0.05 ) after high intensity exercise in T. GPx activity increased in the T group as compared to the UT group, after exercise in moderate ( 25.90 +/- 3.79 to 15.05 +/- 3.23 nM x min ( -1 ) x mg protein ( -1 ) ) and high ( 21.75 +/- 4.91 to 12.1 +/- 2.46 nM x min ( -1 ) x mg protein ( -1 ) ) intensities ( p < 0.05 )
Kim et al., J Biol Chem 2006 : The beta-catenin transactivation domain bound directly to isolated MED12 and intact Mediator both in vitro and in vivo, and Mediator was recruited to Wnt-responsive genes in a beta-catenin dependent manner
Chen et al., Assay Drug Dev Technol 2006 : Compound 1 did not affect the tumor necrosis factor-alpha- or lipopolysaccharide induced TRAP-luciferase response , suggesting selective inhibition of the RANKL induced pathway
Black et al., Mol Cell 2006 : Using purified proteins, we found that the Mediator regulates this assembly process by binding to p300 and TFIID
Zhou et al., Mol Cell Biol 2006 : We propose that activated Gli3 physically targets the MED12 interface within Mediator in order to functionally reverse Mediator dependent suppression of Shh target gene transcription
Wu et al., Hum Mol Genet 2006 : The FPC C-terminus substantially stimulated PC2 channel activity in the presence of KIF3B , whereas FPC or KIF3B alone had no effect
Duarte et al., Biochem Biophys Res Commun 2006 : Similarly to thrombin, FGF1 expression and release were induced by TRAP , a specific oligopeptide agonist of PAR1 ... Similarly to thrombin, FGF1 expression and release were induced by TRAP , a specific oligopeptide agonist of PAR1
Gopalakrishnan et al., Biochem Cell Biol 2006 (Diabetes Mellitus, Experimental) : In the vertebrae, TRAP activity was elevated as a result of diabetes, but this was prevented by insulin or estradiol
Speziani et al., Eur J Immunol 2007 : IL-2, IFN-gamma and IL-4 inhibit TRAP and bone resorption activities contrary to IL-10, which enhances both activities ... IL-2 , IFN-gamma and IL-4 inhibit TRAP and bone resorption activities contrary to IL-10, which enhances both activities ... IL-2, IFN-gamma and IL-4 inhibit TRAP and bone resorption activities contrary to IL-10, which enhances both activities
Arriagada et al., J Steroid Biochem Mol Biol 2007 : We find that both mutation of serine 208 to aspartic acid ( VDRS208D ) or phosphorylation of VDR by CKII enhance the interaction of VDR with DRIP205 in the presence of 1alpha,25-dihydroxy Vitamin D3
Fox et al., Frontiers in bioscience : a journal and virtual library 2007 : Evidence from our laboratory suggests that Nescient helix-loop-helix 2 (Nhlh2) regulates the transcription of PC1 and PC2 , possibly in conjunction with the leptin stimulated transcription factor, STAT3
Patel et al., Platelets 2007 : Platelet reactivity was evaluated by measuring platelet aggregation, platelet leukocyte aggregates ( PLA ) formation in response to a 6-mer thrombin receptor agonist peptide (TRAP) at a final concentration of 40 microM and flow cytometry determined P-selectin expression induced by ADP, TRAP and arachidonic acid ( AA )
Nillni et al., Endocrinology 2007 (Obesity) : Because biosynthesis of mature peptides in response to leptin requires prohormone processing, it is hypothesized that leptin might regulate hypothalamic PC1/3 and PC2 expression, ultimately leading to coordinated processing of prohormones into mature peptides
Boldt et al., Vox Sang 2007 : B-HES but not NB-HES increases the expression of activated platelet GP IIb/IIIa induced by ADP or TRAP
Flaujac et al., Thromb Haemost 2007 (Postoperative Hemorrhage) : Platelet activation marker expression ( CD62P and activated alphaIIbbeta3 ) induced by ADP or TRAP was lower after CPB than before CPB, suggesting a deleterious effect of normothermic CPB on platelet function
Liu et al., Mol Cell Biol 2008 : STAF65gamma is required for SPT3/STAGA interaction with core Mediator and for MYC recruitment of SPT3, TAF9, and core Mediator components to the TERT promoter but is dispensable for MYC recruitment of TRRAP, GCN5, and p300 and for acetylation of nucleosomes and loading of TFIID and RNA polymerase II on the promoter
Ge et al., Mol Cell Biol 2008 : These results indicate that there is a conditional requirement for MED1/TRAP220 and that a direct interaction between PPARgamma and Mediator through MED1/TRAP220 is not essential either for PPARgamma stimulated adipogenesis or for PPARgamma target gene expression in cultured fibroblasts ... As Mediator is apparently essential for PPARgamma transcriptional activity, our data indicate the presence of alternative mechanisms for Mediator recruitment, possibly through intermediate cofactors or other cofactors that are functionally redundant with MED1/TRAP220
Ariyoshi et al., J Cell Biochem 2008 (Bone Resorption) : Heparin suppressed TRAP positive multinucleated cell formation and TRAP activity induced by RANKL , whereas the other GAGs showed no effects on osteoclast differentiation
Christersson et al., Journal of thrombosis and haemostasis : JTH 2008 : TRAP and P-selectin increased TF, IL8 , and MCP-1 mRNA in whole blood and purified monocytes
Belakavadi et al., Mol Cell Biol 2008 : Here, we report that phosphorylation of MED1 by mitogen activated protein kinase-extracellular signal regulated kinase ( MAPK-ERK ) promotes its association with Mediator
Mancino et al., Dig Liver Dis 2009 (Bile Duct Neoplasms...) : 17beta-Estradiol stimulated proliferation of HuH-28 cells was blocked by 70 % by VEGF-TRAP , a receptor based VEGF inhibitor
Contreras-Levicoy et al., FEBS J 2008 : Activation of transcription by PC4 was dependent on the Mediator complex and TFIIA, but was independent of TATA binding protein associated factor
Kauskot et al., Frontiers in bioscience : a journal and virtual library 2008 : The disintegrin inhibited the binding of FITC-fibrinogen and FITC-PAC-1 to ADP stimulated platelets and inhibited ADP-, TRAP- and collagen induced aggregation of murine, rabbit or human platelets ... The disintegrin inhibited the binding of FITC-fibrinogen and FITC-PAC-1 to ADP stimulated platelets and inhibited ADP-, TRAP- and collagen induced aggregation of murine, rabbit or human platelets
Tsutsui et al., Genes Cells 2008 : While the role of CDK8 has been studied extensively, little is known of the role of CDK11 in Mediator
Celić et al., J Biol Chem 2008 : Our results suggest that PC2-CC is involved in PC2 oligomerization, and PC2-EF is a Ca2+-sensitive switch
Thiaville et al., Nucleic Acids Res 2008 : It is unclear whether Mediator complex in yeast is necessary for all RNA polymerase II (Pol II) transcription or if it is limited to genes activated by environmental stress
Chauleur et al., Journal of thrombosis and haemostasis : JTH 2008 (Postpartum Hemorrhage) : From values obtained 6-9 months after delivery, low ( but not-deficient ) levels of fibrinogen, von Willebrand factor (VWF) antigen, factor (F) XI, platelet CD42b, TRAP induced increase of platelet CD41a and high values of serum residual prothrombin activity or closure aperture times using the collagen-ADP cartridge on the PFA-100 system, and blood group O, were independently associated with a significant risk of severe PPH. Being positive for at least two of these eight variables was found in 1.6 %, 3.5 % and 20.8 % of the women from the C, the NS-PPH and the S-PPH groups, respectively, the odds ratio for S-PPH in such a case being 16.4, 95 % CI ( 6.5-41 ), P < 0.0001
Geng et al., Proc Natl Acad Sci U S A 2008 (Calcium Signaling) : Single channel studies showed that interaction with Stx5 specifically reduces PC2 channel activity
Katz et al., Mol Cell Biol 2009 : By binding and transactivation studies, we found that Pax6 indirectly regulates PC2 gene transcription through cMaf and Beta2/NeuroD1 while it activates the 7B2 gene both directly and indirectly through the same transcription factors, cMaf and Beta2/NeuroD1
Bae et al., Thromb Haemost 2009 : Furthermore, similar to activated protein C, both thrombin and TRAP activated Rac1 and inhibited the activation of RhoA and NF-kappaB pathways in response to TNF-alpha in cells pretreated with protein C-S195A ... Furthermore, similar to activated protein C, both thrombin and TRAP activated Rac1 and inhibited the activation of RhoA and NF-kappaB pathways in response to TNF-alpha in cells pretreated with protein C-S195A
Chandler et al., Thromb Res 2010 (Coronary Disease) : Platelet activation by adenosine diphosphate ( ADP ) or thrombin agonist peptide (TRAP) increased CD62P and CD40L surface density in the presence of aspirin by 1.9 - 2.8 -fold ... Platelet activation by adenosine diphosphate ( ADP ) or thrombin agonist peptide (TRAP) increased CD62P and CD40L surface density in the presence of aspirin by 1.9 - 2.8 -fold
Kim et al., J Cell Physiol 2009 (Bone Resorption) : We found that saurolactam was one of the compounds inhibiting the RANKL induced osteoclastogenesis ; it significantly inhibited the RANKL induced TRAP activity and formation of multinucleated osteoclasts without any cytotoxicity
Konda et al., Journal of inflammation (London, England) 2009 : The inhibitory effect of RIAA on inflammatory markers was assessed by measuring nitric oxide in LPS stimulated RAW 264.7 cells, RANKL mediated TRAP activity in transformed osteoclasts, and TNF-alpha/IL-1beta mediated MMP-13 expression in SW1353 cells ... In addition, RIAA inhibited NF-kappaB mediated inflammatory markers in various cell models, including nitric oxide in LPS stimulated RAW 264.7 cells, RANKL mediated TRAP activity in transformed osteoclasts, and TNF-alpha/IL-1beta mediated MMP-13 expression in SW1353 human chondrosarcoma cells ... In addition, RIAA inhibited NF-kappaB mediated inflammatory markers in various cell models, including nitric oxide in LPS stimulated RAW 264.7 cells, RANKL mediated TRAP activity in transformed osteoclasts, and TNF-alpha/IL-1beta mediated MMP-13 expression in SW1353 human chondrosarcoma cells
Mazière et al., J Cell Physiol 2009 (Bone Resorption) : Finally, OxLDL also prevented RANKL induced TRAP activity and RANKL induced bone resorbing activity of human peripheral blood mononuclear cells
Yu et al., J Biol Chem 2009 : To determine whether IL-4 regulates TRAP promoter activity, RAW264.7 cells were transfected with a TRAP promoter-luciferase reporter ... STAT6VT did not inhibit the potent up-regulation of TRAP promoter activity caused by overexpression of NFATc1, PU.1, and microphthalmia transcription factor, downstream targets of macrophage colony stimulating factor and RANKL ... Knockdown of NFATc1 by short interfering RNA caused TRAP expression to be down-regulated, and ectopic expression of NFATc1 abrogated the IL-4 induced down-regulation of TRAP ... These results suggest that STAT6 plays two distinct roles in TRAP expression ... However, in the absence of RANKL and osteoclast differentiation, STAT6 binds the TRAP promoter after IL-4 treatment and directly enhances TRAP expression ... However, in the absence of RANKL and osteoclast differentiation, STAT6 binds the TRAP promoter after IL-4 treatment and directly enhances TRAP expression
Wezensky et al., Gene 2010 : Co-immunoprecipitation and promoter-reporter studies reveal that the effect of PC2 on PLAGL2 target promoter activity was conferred via the C-terminus of PLAGL2, the region that is required for PC2 binding and contains the PLAGL2 activation domain
Vincourt et al., Cancer Res 2010 (Bone Neoplasms...) : In contrast, soluble PC1CP, but not PC2CP , induced the migration of EAhy926 cells and increased vascular endothelial growth factor ( VEGF ) and CXCR4 expression in chondrocytes ... In contrast, soluble PC1CP, but not PC2CP , induced the migration of EAhy926 cells and increased vascular endothelial growth factor ( VEGF ) and CXCR4 expression in chondrocytes ... Soluble PC2CP also increased VEGF expression, but along with a more pronounced effect on CXCR4 and matrix metalloproteinase 13 expression
Crespin et al., Blood Coagul Fibrinolysis 2009 : Inhibition of Cdc42 and Rac1 by toxin B from Clostridium difficile, that suppresses PAK1/2 activation induced by TRAP and collagen or by A23187 in the presence of calpeptin, decreases polymerization of actin, lamellipodia and filopodia formation and interferes with the shedding of microvesicles
Carey et al., Cold Spring Harbor protocols 2010 : INTRODUCTION : The Mediator ( Med ) complex plays a key role in promoter-specific activation of transcription by RNA polymerase II (Pol II)
Kunin et al., PloS one 2010 : Mediator of DNA damage checkpoint 1 ( MDC1 ) contributes to high NaCl induced activation of the osmoprotective transcription factor TonEBP/OREBP
Xu et al., EMBO Rep 2011 (Alzheimer Disease) : Mediator , in a MED12 dependent manner, occupies only AICD bound promoter DNA, indicating that the AICD recruits Mediator to activate transcription
Franco et al., Exp Cell Res 2011 (Bone Resorption) : Furthermore, MMP-9 enzyme inhibitor also attenuated both RANKL induced osteoclastogenesis and up-regulation of TRAP and cathepsin K mRNA expression, indicating that MMP-9 enzyme action is engaged in the promotion of RANKL induced osteoclastogenesis ... Furthermore, MMP-9 enzyme inhibitor also attenuated both RANKL induced osteoclastogenesis and up-regulation of TRAP and cathepsin K mRNA expression, indicating that MMP-9 enzyme action is engaged in the promotion of RANKL induced osteoclastogenesis ... Finally, Dox treatment abrogated RANKL induced osteoclastogenesis and TRAP activity in mouse calvaria along with the suppression of MMP9 enzyme activity, again without affecting the expression of MMP9 protein
Lin et al., Genes Dev 2011 : Studies in depleted extracts showed that the Mediator coactivator complex, which controls PIC assembly, is also necessary for CHD1 recruitment
Ang et al., PLoS Biol 2012 : Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80 , which places Mediator genetically upstream of the activator Gal4 ... Furthermore, we have found that Mediator controls the galactose induced protein degradation of Gal80, which places Mediator genetically upstream of the activator Gal4
Tai et al., Blood 2012 (Multiple Myeloma...) : PCI-32765 blocked RANKL/M-CSF induced phosphorylation of Btk and downstream PLC-?2 in OCs, resulting in diminished TRAP5b ( ED50 = 17 nM ) and bone resorption activity ... PCI-32765 blocked RANKL/M-CSF induced phosphorylation of Btk and downstream PLC-?2 in OCs, resulting in diminished TRAP5b ( ED50 = 17 nM ) and bone resorption activity
Zhang et al., Biochimie 2012 (Bone Resorption) : Besides, RANKL induced osteoclast formation from RAW 264.7 cells and the expression of TRAP , CA II, CTSK and MMP-9 was significantly reduced by the treatment of 25 µM HEF and 17ß-estradiol ( ES ), and the inhibitory strength increases in the order TF < ES < ICA < BS, which was blocked by ICI182780 suggesting that the regulation of osteoclast activity might be ER dependent
Yu et al., Inflammation 2012 : Removal of Ca ( 2+ ) by Ca ( 2+ ) chelator BAPTA or inhibition of protein kinase A (PKA) by the PKA inhibitors H-89 each partially reduced PC(2)s induced mucin secretion
Chitu et al., Blood 2012 (Bone Diseases, Metabolic...) : PSTPIP2 tyrosine phosphorylation and a functional F-BAR domain were essential for PSTPIP2 inhibition of TRAP expression and osteoclast precursor fusion, whereas interaction with PEST-type phosphatases was only required for suppression of TRAP expression
Povolny et al., Exp Hematol 1990 : In addition, the effect of 1,25-dihydroxyvitamin D3 ( calcitriol ) on CSF induced colony growth and TRAP expression was also determined
Lewis et al., Clin Exp Allergy 2013 : Mediator release induced by SCF was accompanied by the up-regulation of the activation marker, CD63
Naidu et al., Chem Biol Interact 2013 (Arthritis, Experimental...) : The peptide YR-11 ( YLEIEFSLKHR ), obtained by direct substitution of cysteine with a serine residue in the template sequence, significantly ( p < 0.05 ) inhibited RANK-RANKL binding, and RANKL induced TRAP activity and formation of multinucleated osteoclasts without any cytotoxicity
Gremmel et al., Translational research : the journal of laboratory and clinical medicine 2013 (Obesity...) : Finally, PAR-1 mediated platelet activation as assessed by expression of P-selectin and activated GPIIb/IIIa in response to TRAP-6 was significantly more pronounced in obese patients than in patients without obesity ( both P = 0.02 ) ... Finally, PAR-1 mediated platelet activation as assessed by expression of P-selectin and activated GPIIb/IIIa in response to TRAP-6 was significantly more pronounced in obese patients than in patients without obesity ( both P = 0.02 )
Kim et al., J Biol Chem 2013 : Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16 ... Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16
Verger et al., Nucleic Acids Res 2013 : We further show that depletion of MED25 disrupts the association of ERM with the Mediator in vitro
Chen et al., Int J Biochem Cell Biol 2013 : When stimulated with 12-O-tetradecanoylphorbol 13-acetate ( TPA ) or CD437, this TR3-TRAP? interaction not only induced Ca ( 2+ ) depletion in the endoplasmic reticulum ( ER ) but also promoted the expression of the proapoptotic transcriptional regulator CHOP
Galbraith et al., Cell 2013 (Neoplasms) : HIF1A Employs CDK8-Mediator to Stimulate RNAPII Elongation in Response to Hypoxia ... HIF1A induces binding of CDK8-Mediator and the super elongation complex ( SEC ), containing AFF4 and CDK9, to alleviate RNAPII pausing
Šedý et al., J Immunol 2013 (Inflammation) : CD160 Activation by Herpesvirus Entry Mediator Augments Inflammatory Cytokine Production and Cytolytic Function by NK Cells
Liou et al., PloS one 2013 (Osteoporosis) : In addition, KMUP-1 inhibited RANKL induced activation of signaling molecules ( Akt, MAPKs, calcium and NF-?B ), mRNA expression of osteoclastogensis associated genes ( TRAP , MMP-9, Fra-1, and cathepsin K ) and transcription factors ( c-Fos and NFATc1 )
Newman et al., Endocrinology 1985 : Mediator generation was rapid, with a half-time of approximately 45 sec and was insulin dose dependent
Shankar et al., J Biol Chem 1994 : Furthermore, the stimulation of E-selectin cell surface expression and the steady-state E-selectin mRNA levels by thrombin and TRAP were comparable ... Northern analysis, however, revealed that TRAP at 100 microM stimulated PDGF-A and -B chain mRNA expression to a level similar to that induced by thrombin ... Northern analysis, however, revealed that TRAP at 100 microM stimulated PDGF-A and -B chain mRNA expression to a level similar to that induced by thrombin
Korthäuer et al., Nature 1993 (Hypergammaglobulinemia...) : The resultant failure of TRAP to interact with CD40 on functionally intact B cells is responsible for the observed immunoglobulin isotype defect in HIGM1
Nakamura et al., Am J Physiol 1995 : Plasmin inhibited the thrombin evoked increase in cytosolic Ca2+ and also inhibited the Ca2+ response to the tethered peptide TRAP-6 of the thrombin receptor
Kruse et al., Am J Physiol 1995 : Receptor operated effects of alpha-thrombin and of the thrombin receptor activating peptide TRAP14 on cytoplasmic Ca2+ concentration ([Ca2+]i) were examined in fura 2-loaded endothelial cells
Day et al., DNA Cell Biol 1995 : Maintained PC1 and PC2 expression in the AtT-20 variant cell line 6T3 lacking regulated secretion and POMC : restored POMC expression and regulated secretion after cAMP treatment
Maruyama et al., Biochem Biophys Res Commun 1994 : TRAP induced rapid morphological changes in HUVECs, with marked increase in the release of prostacyclin, endothelin , platelet activating factor, tissue type plasminogen activator, and plasminogen activator inhibitor-1
de la Piedra et al., Horm Metab Res 1993 (Bone Resorption) : In the present work, 10 ( -7 ) M PTH ( 1-34 ), 10 ( -7 ) M PTHrP ( 1-34 ) and 10 ( -8 ) M or 10 ( -10 ) M 1,25 ( OH ) 2D3 produced a significant increase in TRAP activity, when these agonists were added to the calvaria culture
Kasono et al., Bone Miner 1993 (Bone Resorption) : The minimum effective inhibitory concentration was 0.01 ng/ml, and 1 ng/ml IL-4 completely inhibited TRAP ( + ) MNC formation
Jenkins et al., J Cell Sci 1995 : The use of alanine substituted peptides demonstrated that the Ca2+ response was due to TRAP stimulation of a receptor other than the proteolytically activated thrombin receptor
Kanthou et al., Thromb Haemost 1995 : Examination of thrombin and TRAP treated cells by immunofluorescence staining followed by computer assisted image analysis revealed that thrombin and to a lesser extent TRAP induced PDGF-AA protein expression
Michelson et al., Blood 1996 : First, although only the GPIb alpha subunit of this putative complex is known to be directly linked to the platelet cytoskeleton via actin binding protein, cytochalasin B inhibited the ADP/epinephrine-, cathepsin G-, and TRAP induced decrease in platelet surface GPV
Jiang et al., Circ Res 1996 : Differences in the kinetics and magnitude of thrombin- and TRAP dependent inositol phosphate ( IP ) accumulation are paralleled by differences in the kinetics and magnitude of thrombin- and TRAP dependent activation of MAPK
Blackhart et al., J Biol Chem 1996 : PAR-2 was also found to be responsive to TRAP ( EC50 = 1 microM ) but was unresponsive to Xenopus TRAP ( 50 microM )
Satoh et al., Biochem Biophys Res Commun 1996 : Both thrombin and TRAP induced translocation of p60c-src and p54/58lyn to cytoskeleton in an aggregation dependent manner ... Both thrombin and TRAP induced translocation of p60c-src and p54/58lyn to cytoskeleton in an aggregation dependent manner ... Both thrombin and TRAP induced translocation of p60c-src and p54/58lyn to cytoskeleton in an aggregation dependent manner ... In contrast, TRAP , even at concentrations as high as 100 mu M, did not induce p72syk translocation to cytoskeleton
Faraday et al., Thromb Haemost 1997 : Similarly, the number of GPIIb-IIIa receptors capable of binding PAC-1 in response to ADP and TRAP was 50 % to 80 % greater in women than men
Jansen et al., Biochem J 1997 (Insulinoma) : Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity
Johanning et al., J Biol Chem 1998 : Analysis of proenkephalin reaction products using immunoblotting and gel permeation chromatography demonstrated that PC2 can directly cleave proenkephalin and that the generation of small opioid peptides from intermediates is mediated almost entirely by PC2 rather than by PC1 ... Analysis of proenkephalin reaction products using immunoblotting and gel permeation chromatography demonstrated that PC2 can directly cleave proenkephalin and that the generation of small opioid peptides from intermediates is mediated almost entirely by PC2 rather than by PC1
Xirasagar et al., J Biol Chem 1998 : This conclusion is based on the effects of temperature and Mg2+ on the affinity of TRAP for RNAs with CC spacers combined with UV hyperchromicity and circular dichroism
Han et al., Mol Cell Biol 1999 : The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription