There is a very high volume of traffic coming from your site (IP address 3.236.218.88) as of Sun Oct 17 22:51:26 2021 (California time). So that other users get a fair share of our bandwidth, we are putting in a delay of 14.5 seconds before we service your request. This delay will slowly decrease over a half hour as activity returns to normal. This high volume of traffic is likely due to program-driven rather than interactive access, or the submission of queries on a large number of sequences. If you are making large batch queries, please write to our genome@soe.ucsc.edu public mailing list and inquire about more efficient ways to access our data. If you are sharing an IP address with someone who is submitting large batch queries, we apologize for the inconvenience. To use the genome browser functionality from a Unix command line, please read <a href='http://genome.ucsc.edu/FAQ/FAQdownloads.html#download36'>our FAQ</a> on this topic. For further help on[...] Restr Enzymes Track Settings
Restr Enzymes Track Settings
 
Restriction Enzymes from REBASE   (All Mapping and Sequencing tracks)

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Filter display by enzymes (separate with commas):


Data last updated: 2011-12-31; REBASE version 201

Description

This track shows restriction enzyme recognition sites. At different browser zoom levels (categorized in the table below by the number of bases visible), different sets of enzymes are shown. "Base-level" refers to the browser zoom level at which individual bases are displayed in the Base Position track. Displays of custom enzyme sets override the table: in these cases, the restriction sites are consistent across all zoom levels. "Primary" enzymes are the canonical isoschizomer for each cutsite, usually the first isolated or most readily available.

The base-level display shows the detail of the cutting pattern of the enzyme as well as the presence of any ambiguous or redundant nucleotides in the cutsite. The four standard bases, G, A, T and C, are displayed in black, whereas non-unique bases using IUPAC designations (R, Y, N, etc) are displayed in blue.

The term "isoligamer" in the enzyme details section was coined to designate enzymes that produce single-stranded overhanging "sticky" ends that are ligatable to each other.

Display

Zoom level (in bases)Enzyme set displayed
Base-level All "primary" enzymes from REBASE
Base-level - 2,999 Restriction sites for "primary" enzymes five bases and larger.
3,000 - 19,999 Restriction sites for "primary" enzymes six bases and larger
20,000 - 249,999 The following set of commonly-used enzymes: ClaI, BamHI, BglII, EcoRI, EcoRV, HindIII, PstI, SalI, SmaI, XbaI, KpnI, SacI, SphI
> 250,000 None displayed

Credits

Thanks to REBASE for providing these data.