Optical Map Analysis: fragments predicted from reference

Description

Optical Mapping (OM) is a system that produces ordered restriction maps from individual molecules of genomic DNA. Each single-molecule restriction map (rMap) is a direct measurement of the source genome, free from biases introduced by cloning, amplification, or hybridization. This track presents the set of OM fragments that would be expected based on the reference sequence, based on an in silico restriction digest. It is complemented by the 'Optical Map Analysis: Alignments' and 'Optical Map Analysis: Deletions' tracks, which display experimental OM data for individual cell lines.

Display Conventions and Configuration

OM produces ordered restriction maps. This track shows restriction fragments as a result of in silico restriction digest of the reference assembly. Restriction cut-sites are displayed as vertical lines.

Methods

Optical Mapping. See References section below for further details.

Credits

OM data, analysis and alignments generated by the Schwartz lab, University of Wisconsin and used by the Genome Reference Consortium to improve reference assemblies. Contact us via genomereference.org or by emailing grc-help@sanger.ac.uk.

References

• High-resolution human genome structure by single-molecule analysis. (2010) PNAS 107(24):10848-53.
• Lineage-specific biology revealed by a finished genome assembly of the mouse. (2009) PLos Biol 7(5):e1000112.
• Modernizing Reference Genome Assemblies. (2011) PLoS Biol 9(7): e1001091.