Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2002, PMID: 12459498

NF-kappaB activator Act1 associates with IL-1/Toll pathway adaptor molecule TRAF6.

Kanamori, Mutsumi; Kai, Chikatoshi; Hayashizaki, Yoshihide; Suzuki, Harukazu

NF-kappaB activator 1 (Act1), also called CIKS, is a recently identified protein with NF-kappaB and AP-1 activation activities through its association with the IkappaB kinase complex. We identified and confirmed that Act1 interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6); notably, Act1 binds to TRAF6 only among TRAF family proteins. The amino-terminal half of Act1 is required for its interaction with the TRAF domain. Act1-mediated NF-kappaB activation was inhibited by a dominant-negative mutant of TRAF6 in a dose-dependent manner, and IL-1-induced NF-kappaB activation was inhibited by a high level of Act1 expression. Our results suggest that Act1 is involved in IL-1/Toll-mediated signaling through TRAF6.

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Text Mining Data

NF-kappaB ⊣ TRAF6: " Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression "

NF-kappaB ⊣ Act1: " Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression "

NF-kappaB ⊣ IL-1: " Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression "

Manually curated Databases

  • IRef Biogrid Interaction: TRAF6 — TRAF3IP2 (direct interaction, two hybrid)
  • IRef Biogrid Interaction: TRAF6 — TRAF3IP2 (direct interaction, pull down)
  • IRef Biogrid Interaction: TRAF6 — TRAF3IP2 (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: TRAF6 — TRAF3IP2 (in vitro)
  • IRef Hprd Interaction: TRAF6 — TRAF3IP2 (in vivo)
  • IRef Ophid Interaction: TRAF6 — TRAF3IP2 (aggregation, confirmational text mining)
In total, 1 gene pairs are associated to this article in curated databases