J Leukoc Biol 2008,
PMID: 18339893
Ghavami, Saeid; Rashedi, Iran; Dattilo, Brian M; Eshraghi, Mehdi; Chazin, Walter J; Hashemi, Mohammad; Wesselborg, Sebastian; Kerkhoff, Claus; Los, Marek
The complex formed by two members of the S100 calcium-binding protein family, S100A8/A9, exerts apoptosis-inducing activity against various cells, especially tumor cells. Here, we present evidence that S100A8/A9 also has cell growth-promoting activity at low concentrations. Receptor of advanced glycation end product (RAGE) gene silencing and cotreatment with a RAGE-specific blocking antibody revealed that this activity was mediated via RAGE ligation. To investigate the signaling pathways, MAPK phosphorylation and NF-kappaB activation were characterized in S100A8/A9-treated cells. S100A8/A9 caused a significant increase in p38 MAPK and p44/42 kinase phosphorylation, and the status of stress-activated protein kinase/JNK phosphorylation remained unchanged. Treatment of cells with S100A8/A9 also enhanced NF-kappaB activation. RAGE small interfering RNA pretreatment abrogated the S100A8/A9-induced NF-kappaB activation. Our data indicate that S100A8/A9-promoted cell growth occurs through RAGE signaling and activation of NF-kappaB.
Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
Document information provided by NCBI PubMed
Text Mining Data
p44/42 → S100A8/A9: "
S100A8/A9 caused a significant increase in p38 MAPK and
p44/42 kinase phosphorylation, and the status of stress activated protein kinase/JNK phosphorylation remained unchanged
"
p44/42 → S100A8/A9: "
S100A8/A9 caused a significant increase in p38 MAPK and p44/42 kinase phosphorylation, and the status of stress activated protein kinase/JNK phosphorylation remained unchanged
"
MAPK → S100A8/A9: "
S100A8/A9 caused a significant increase in p38 MAPK and p44/42 kinase phosphorylation, and the status of stress activated protein kinase/JNK phosphorylation remained unchanged
"
NF-kappaB → S100A8/A9: "
RAGE small interfering RNA pretreatment abrogated the S100A8/A9 induced NF-kappaB activation
"
RAGE → NF-kappaB: "
Our data indicate that S100A8/A9 promoted cell growth occurs through RAGE signaling and activation of NF-kappaB
"
Manually curated Databases
No curated data.