Apoptosis 2008,
PMID: 19002588
Oh, Jung Hwa; Lee, Tae-Jin; Kim, Sang Hyun; Choi, Yung Hyun; Lee, Sang Han; Lee, Jin Man; Kim, Young-Ho; Park, Jong-Wook; Kwon, Taeg Kyu
Withaferin A, a major chemical constituent of Withania somnifera, has been reported for its tumor cell growth inhibitory activity, antitumor effects, and impairing metastasis and angiogenesis. The mechanism by which withaferin A initiates apoptosis remains poorly understood. In the present report, we investigated the effect of withaferin A on the apoptotic pathway in U937 human promonocytic cells. We show that withaferin A induces apoptosis in association with the activation of caspase-3. JNK and Akt signal pathways play crucial roles in withaferin A-induced apoptosis in U937 cells. Furthermore, we have shown that overexpression of Bcl-2 and active Akt (myr-Akt) in U937 cells inhibited the induction of apoptosis, activation of caspase-3, and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells.
Diseases/Pathways annotated by Medline MESH: Leukemia
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Text Mining Data
NF-kappaB ⊣ Akt: "
Furthermore, we have shown that overexpression of Bcl-2 and active Akt ( myr-Akt ) in U937 cells inhibited the induction of apoptosis, activation of caspase-3, and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and
Akt pathways and
inhibition of
NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells
"
caspase-3 → Akt: "
Furthermore, we have shown that overexpression of Bcl-2 and active Akt ( myr-Akt ) in U937 cells inhibited the induction of apoptosis, activation of caspase-3 , and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells
"
caspase-3 → Bcl-2: "
Furthermore, we have shown that overexpression of Bcl-2 and active Akt ( myr-Akt ) in U937 cells inhibited the induction of apoptosis, activation of caspase-3 , and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells
"
PLC-gamma1 → caspase-3: "
Furthermore, we have shown that overexpression of Bcl-2 and active Akt ( myr-Akt ) in U937 cells inhibited the induction of apoptosis, activation of caspase-3 , and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells
"
Manually curated Databases
No curated data.