J Biol Chem 2011,
PMID: 21454471
Zucchelli, Silvia; Marcuzzi, Federica; Codrich, Marta; Agostoni, Elena; Vilotti, Sandra; Biagioli, Marta; Pinto, Milena; Carnemolla, Alisia; Santoro, Claudio; Gustincich, Stefano; Persichetti, Francesca
Huntington disease (HD) is a neurodegenerative disorder caused by an expansion of polyglutamines in the first exon of huntingtin (HTT), which confers aggregation-promoting properties to amino-terminal fragments of the protein (N-HTT). Mutant N-HTT aggregates are enriched for ubiquitin and contain ubiquitin E3 ligases, thus suggesting a role for ubiquitination in aggregate formation. Here, we report that tumor necrosis factor receptor-associated factor 6 (TRAF6) binds to WT and polyQ-expanded N-HTT in vitro as well as to endogenous full-length proteins in mouse and human brain in vivo. Endogenous TRAF6 is recruited to cellular inclusions formed by mutant N-HTT. Transient overexpression of TRAF6 promotes WT and mutant N-HTT atypical ubiquitination with Lys(6), Lys(27), and Lys(29) linkage formation. Both interaction and ubiquitination seem to be independent from polyQ length. In cultured cells, TRAF6 enhances mutant N-HTT aggregate formation, whereas it has no effect on WT N-HTT protein localization. Mutant N-HTT inclusions are enriched for ubiquitin staining only when TRAF6 and Lys(6), Lys(27), and Lys(29) ubiquitin mutants are expressed. Finally, we show that TRAF6 is up-regulated in post-mortem brains from HD patients where it is found in the insoluble fraction. These results suggest that TRAF6 atypical ubiquitination warrants investigation in HD pathogenesis.
Diseases/Pathways annotated by Medline MESH: Huntington Disease
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
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IRef Biogrid Interaction:
UBC
—
TRAF6
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HTT
—
UBC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HTT
—
TRAF6
(physical association, affinity chromatography technology)
In total, 3 gene pairs are associated to this article in curated databases