Gene interactions and pathways from curated databases and text-mining
Biochem Biophys Res Commun 1997, PMID: 9125224

Redox regulation of lipopolysaccharide (LPS)-induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells.

Tanaka, C; Kamata, H; Takeshita, H; Yagisawa, H; Hirata, H

LPS-induced expression of the IL-8 gene was markedly enhanced by H2O2 or by deprivation of the cellular antioxidant glutathione by L-buthionine-(S,R)-sulfoximine (BSO) in human astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-cysteine (NAC) and other antioxidants. Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC. These results suggest that LPS-induced IL-8 gene expression is regulated by cellular redox via modulation of these transcription factors.

Diseases/Pathways annotated by Medline MESH: Astrocytoma
Document information provided by NCBI PubMed

Text Mining Data

interleukin-8 (IL-8) ⊣ AP-1: " Redox regulation of lipopolysaccharide (LPS) induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells "

interleukin-8 (IL-8) ⊣ NF kappa B: " Redox regulation of lipopolysaccharide (LPS) induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells "

interleukin-8 (IL-8) → lipopolysaccharide (LPS): " Redox regulation of lipopolysaccharide (LPS) induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells "

IL-8 → LPS: " LPS induced expression of the IL-8 gene was markedly enhanced by H2O2 or by deprivation of the cellular antioxidant glutathione by L-buthionine- ( S, R ) -sulfoximine ( BSO ) in human astrocytoma U373 cells "

IL-8 ⊣ NAC: " Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC "

LPS ⊣ NAC: " Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC "

AP-1 → LPS: " Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC "

IL-8 → LPS: " Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC "

NF kappa B → LPS: " Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC "

IL-8 → LPS: " These results suggest that LPS induced IL-8 gene expression is regulated by cellular redox via modulation of these transcription factors "

Manually curated Databases

No curated data.