Mol Cell Biol 1997,
PMID: 9154803
Stang, S; Bottorff, D; Stone, J C
v-H-ras effector mutants have been assessed for transforming activity and for the ability of the encoded proteins to interact with Raf-1-, B-Raf-, byr2-, ralGDS-, and CDC25-encoded proteins in the yeast two-hybrid system. Transformation was assessed in rat2 cells as well as in a mutant cell line, rv68BUR, that affords a more sensitive transformation assay. Selected mutant Ras proteins were also examined for their ability to interact with an amino-terminal fragment of Raf-1 in vitro. Finally, possible cooperation between different v-H-ras effector mutants and between effector mutants and overexpressed Raf-1 was assessed. Ras transforming activity was shown to correlate best with the ability of the encoded protein to interact with Raf-1. No evidence for cooperation between v-H-ras effector mutants was found. Signaling through the Raf1-MEK-mitogen-activated protein kinase cascade may be the only effector pathway contributing to RAS transformation in these cells.
Diseases/Pathways annotated by Medline MESH: Cell Transformation, Neoplastic
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
-
IRef Biogrid Interaction:
SNRPE
—
HRAS
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
HRAS
—
RALGDS
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
HRAS
—
RASGRF1
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
HRAS
—
RGL4
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
HRAS
—
BRAF
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
RAF1
—
HRAS
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
RAF1
—
HRAS
(direct interaction, pull down)
-
IRef Hprd Interaction:
HRAS
—
CDC25C
(two hybrid)
-
IRef Hprd Interaction:
HRAS
—
RGL4
(in vivo)
-
IRef Hprd Interaction:
HRAS
—
RGL4
(in vitro)
-
IRef Hprd Interaction:
HRAS
—
BRAF
(in vitro)
-
IRef Hprd Interaction:
RAF1
—
HRAS
(in vivo)
-
IRef Hprd Interaction:
RAF1
—
HRAS
(in vitro)
-
IRef Hprd Interaction:
RAF1
—
HRAS
(two hybrid)
In total, 7 gene pairs are associated to this article in curated databases