Biochem Biophys Res Commun 1998,
PMID: 9784408
Sano, H; Nakagawa, N; Chiba, R; Kurasawa, K; Saito, Y; Iwamoto, I
We investigated whether intercellular adhesion molecule-1 (ICAM-1) transduces outside-in signals for the production of chemokines IL-8 and RANTES in endothelial cells. Cross-linking of ICAM-1 induced IL-8 and RANTES mRNA expressions and increased their protein synthesis and secretions in endothelial cells. Furthermore, ICAM-1 cross-linking activated 44- and 42-kDa mitogen-activated protein (MAP) kinases (ERK1 and ERK2) in endothelial cells, as indicated by the electrophoretic mobility shift of MAP kinases on SDS-polyacrylamide gels. Finally, the specific MEK inhibitor PD98059 inhibited ICAM-1-induced IL-8 and RANTES production in endothelial cells. Taken together, these results indicate that stimulation of ICAM-1 induces IL-8 and RANTES production through the activation of 44- and 42-kDa MAP kinases in endothelial cells, suggesting that ICAM-1-induced chemokine production in endothelial cells would further attract and activate leukocytes to induce intense inflammation.
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Text Mining Data
intercellular adhesion molecule-1 → interleukin-8: "
Cross linking of
intercellular adhesion molecule-1 induces
interleukin-8 and RANTES production through the activation of MAP kinases in human vascular endothelial cells
"
IL-8 → ICAM-1: "
Cross linking of ICAM-1 induced IL-8 and RANTES mRNA expressions and increased their protein synthesis and secretions in endothelial cells
"
mitogen activated protein ( MAP ) kinases → ICAM-1: "
Furthermore, ICAM-1 cross linking activated 44- and 42-kDa mitogen activated protein ( MAP ) kinases ( ERK1 and ERK2 ) in endothelial cells, as indicated by the electrophoretic mobility shift of MAP kinases on SDS-polyacrylamide gels
"
mitogen activated protein ( MAP ) kinases → ICAM-1: "
Furthermore, ICAM-1 cross linking activated 44- and 42-kDa mitogen activated protein ( MAP ) kinases ( ERK1 and ERK2 ) in endothelial cells, as indicated by the electrophoretic mobility shift of MAP kinases on SDS-polyacrylamide gels
"
IL-8 → ICAM-1: "
Finally, the specific MEK inhibitor PD98059 inhibited ICAM-1 induced IL-8 and RANTES production in endothelial cells
"
IL-8 → ICAM-1: "
Taken together, these results indicate that stimulation of ICAM-1 induces IL-8 and RANTES production through the activation of 44- and 42-kDa MAP kinases in endothelial cells, suggesting that ICAM-1 induced chemokine production in endothelial cells would further attract and activate leukocytes to induce intense inflammation
"
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