Gene interactions and pathways from curated databases and text-mining
J Interferon Cytokine Res 1998, PMID: 9858321

IP-10 gene transcription by virus in astrocytes requires cooperation of ISRE with adjacent kappaB site but not IRF-1 or viral transcription.

Cheng, G; Nazar, A S; Shin, H S; Vanguri, P; Shin, M L

Transcription of the IP-10 gene requires interferon (IFN)-stimulated response element (ISRE) and kappaB sites to be induced by lipopolysaccharide (LPS), IFN-gamma, virus, and poly(I:C). A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS, poly(I:C), and virus has been reported. We investigated whether viral transcription is required for IP-10 induction and how ISRE interacts with IRF-1 and with two kappaB sites. IP-10 mRNA was induced by Newcastle disease virus and Sendai virus in rat astrocytes and the human astrocytoma U251 cell line. IP-10 was also induced by UV-irradiated virus, which is unable to carry out viral transcription. The minimal IP-10 virus response element (VRE) consists of an ISRE and adjacent kappaB site between -236 and -153, to which p50/p65 NF-kappaB proteins and IRF-like proteins bind. Virus induced NF-kappaB binding to an isolated kappaB sequence adjacent to ISRE. However, no protein binding to isolated ISRE was induced by virus. Virus also induced IP-10 in cells expressing a defective IRF-1 gene. Therefore, effective ISRE activity of IP-10 VRE may require an IRF-like protein binding, which is enhanced by an NF-kappaB heterodimer binding to an adjacent KB site. IRF-1 is not required for virus-induced IP-10 gene expression.

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Text Mining Data

IP-10 → IRF-1: " IP-10 gene transcription by virus in astrocytes requires cooperation of ISRE with adjacent kappaB site but not IRF-1 or viral transcription "

IFN-gamma → IP-10: " Transcription of the IP-10 gene requires interferon ( IFN ) -stimulated response element ( ISRE ) and kappaB sites to be induced by lipopolysaccharide (LPS), IFN-gamma , virus, and poly ( I:C ) "

lipopolysaccharide (LPS) → IP-10: " Transcription of the IP-10 gene requires interferon ( IFN ) -stimulated response element ( ISRE ) and kappaB sites to be induced by lipopolysaccharide (LPS) , IFN-gamma, virus, and poly ( I:C ) "

Stat1 → IFN-gamma: " A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS, poly ( I:C ), and virus has been reported "

Stat1 → IFN regulatory factor-1 (IRF-1): " A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS, poly ( I:C ), and virus has been reported "

Stat1 → LPS: " A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS , poly ( I:C ), and virus has been reported "

IFN-gamma → LPS: " A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS , poly ( I:C ), and virus has been reported "

IFN regulatory factor-1 (IRF-1) → LPS: " A requirement for Stat1 binding to ISRE for IFN-gamma and IFN regulatory factor-1 (IRF-1) binding to ISRE for LPS , poly ( I:C ), and virus has been reported "

IRF-like → NF-kappaB: " Therefore, effective ISRE activity of IP-10 VRE may require an IRF-like protein binding, which is enhanced by an NF-kappaB heterodimer binding to an adjacent KB site "

IP-10 → IRF-1: " IRF-1 is not required for virus induced IP-10 gene expression "

Manually curated Databases

No curated data.