◀ Back to IGF1
IGF1 — PRG2
Pathways - manually collected, often from reviews:
-
OpenBEL Selventa BEL large corpus:
IGF1
→
PRG2
(decreases)
Evidence: We have recently identified an inhibitor of PAPP-A, the precursor form of major basic protein (proMBP). In addition, TGF-beta completely suppressed proMBP expression.
Text-mined interactions from Literome
Harvey et al., J Biol Chem 1999
:
It is likely that the local increase in
IGF-I may
lead to an increase in cartilage type
proteoglycan synthesis and maintenance of the cartilage phenotype
van Susante et al., J Orthop Res 2000
:
Recombinant human
insulin-like growth factor-1 and recombinant human transforming growth factor-beta2
stimulated proteoglycan synthesis in a dose dependent and reversed dose dependent fashion, respectively
Studer et al., Am J Physiol Cell Physiol 2000
(Osteoarthritis) :
SNAP, DETA NONOate, endogenously synthesized NO in Ad-iNOS transduced cells, or IL-1 activation decreased
IGF-I stimulated
proteoglycan synthesis in cartilage and monolayer cultures of chondrocytes
Okuda et al., Spine (Phila Pa 1976) 2001
:
Mechanisms of age related decline in
insulin-like growth factor-I dependent
proteoglycan synthesis in rat intervertebral disc cells ... Age related fluctuations in
insulin-like growth factor-I dependent
proteoglycan synthesis in rat intervertebral disc cells were investigated ... Age related changes in the expression of insulin-like growth factor-I and its receptor were assessed together with
insulin-like growth factor-I dependent
proteoglycan synthesis by the cultured nucleus pulposus cells ... By contrast,
insulin-like growth factor-I dependent
proteoglycan synthesis decreased with age, and the sharpest decline of synthesis was found between 8-week and 40-week cells, although the level of insulin-like growth factor-I/insulin-like growth factor-I receptor gene expression was maintained in 40-week-old animals ... The present findings indicate that the age related decline in insulin-like growth factor-I dependent
proteoglycan synthesis in nucleus pulposus is
caused , at least in part, by the increase in
insulin-like growth factor binding proteins at the early stages of aging, and further suggest that a loss of proteoglycan synthesis during the late stages of aging is caused by the downregulation of insulin-like growth factor-I receptor in addition to an increase in insulin-like growth factor binding proteins ... The present findings indicate that the age related decline in
insulin-like growth factor-I dependent
proteoglycan synthesis in nucleus pulposus is caused, at least in part, by the increase in insulin-like growth factor binding proteins at the early stages of aging, and further suggest that a loss of proteoglycan synthesis during the late stages of aging is caused by the downregulation of insulin-like growth factor-I receptor in addition to an increase in insulin-like growth factor binding proteins
Hiraoka et al., Virchows Arch 2002
(Bone Neoplasms...) :
In vitro, the
proteoglycan synthesis of SW1353 cells was
increased by
IGF-I in a dose dependent manner
Serra et al., Am J Vet Res 2002
(Dog Diseases...) :
Effect of transforming growth factor-beta1,
insulin-like growth factor-I , and hepatocyte growth factor on
proteoglycan production and regulation in canine melanoma cell lines
Jin et al., Arch Biochem Biophys 2003
:
In the absence of tissue shear,
IGF-I augmented protein and
proteoglycan synthesis up to twofold at IGF-I concentrations in the range of 100-300 ng/ml
Studer et al., J Orthop Res 2003
:
Tissues were exposed to NO from iNOS induced by IL-1, and
proteoglycan synthesis in
response to
IGF-1 was evaluated in the presence and absence of cGMP dependent protein kinase ( PKG ) inhibitors
Tesch et al., J Orthop Res 1992
:
When these growth factors were added to articular cartilage explants complexed with their native serum binding proteins ( BPs ), both
IGF-I-BP complex and IGF-II-BP complex
stimulated proteoglycan synthesis to different degrees over a 3-day period
Mitropoulou et al., Biochimie 2004
(Breast Neoplasms) :
IGF-I affects
glycosaminoglycan/proteoglycan synthesis in breast cancer cells through tyrosine kinase dependent and -independent pathways ... The
effect of
IGF on
proteoglycan ( PG ) synthesis by two human breast cancer epithelial cell lines, the ER-positive MCF-7 and the ER-negative BT-20, was studied alone and in combination with genistein
Martin et al., Biorheology 2004
(Osteoarthritis) :
We have found that articular cartilage chondrocytes show strong evidence of senescence with increasing age, including synthesis of smaller more irregular aggrecans ; increased expression of lysosomal beta-galactosidase and telomere erosion ; and decreased
proteoglycan synthesis,
response to the anabolic cytokine
IGF-I , proliferative capacity, and mitochondrial function
De Ceuninck et al., Arthritis Res Ther 2004
(Osteoarthritis) :
It antagonized the inhibitory effect of IGFBP-3 on
IGF-1 dependent
proteoglycan synthesis by rabbit chondrocytes ... The addition of NBI-31772 to human OA chondrocytes resulted in the restoration or potentiation of
IGF-1 dependent
proteoglycan synthesis, depending on the IGF-1 concentrations
Starkman et al., Biochem J 2005
:
A decrease in
IGF-I stimulated
proteoglycan synthesis was also observed upon inhibition of mTOR ( mammalian target of rapamycin ) and p70S6 kinase, both of which are downstream of Akt
Imai et al., Acta Endocrinol (Copenh) 1992
(Eye Diseases...) :
The
effects of
IGF-1 and PDGF on hyaluronan and
proteoglycan synthesis were studied separately and in combination ... Both
IGF-1 and PDGF
increased the synthesis of hyaluronan and
proteoglycan in a dose dependent manner ... In contrast,
IGF-1 stimulated secretion of small
proteoglycan while PDGF had little effect on proteoglycan synthesis in skin fibroblasts ... In contrast,
IGF-1 stimulated secretion of small
proteoglycan while PDGF had little effect on proteoglycan synthesis in skin fibroblasts
Jenniskens et al., Osteoarthritis Cartilage 2006
:
Examine
effects of
insulin-like growth factor 1 (IGF1) , transforming growth factor beta2 ( TGFbeta2 ) and fibroblast growth factor 2 (FGF2) on
proteoglycan and collagen network and biomechanical properties of the newly formed cartilage matrix ...
IGF1 increased collagen and
proteoglycan deposition
Leach et al., Domest Anim Endocrinol 2007
:
In cell culture,
IGF-I stimulated
proteoglycan synthesis and increased the expression of Indian hedgehog (Ihh) and type X collagen, markers of chondrocyte differentiation ... In cell culture,
IGF-I stimulated
proteoglycan synthesis and increased the expression of Indian hedgehog (Ihh) and type X collagen, markers of chondrocyte differentiation
Yin et al., J Biol Chem 2009
:
Oxidative stress inhibits
insulin-like growth factor-I induction of chondrocyte
proteoglycan synthesis through differential regulation of phosphatidylinositol 3-Kinase-Akt and MEK-ERK MAPK signaling pathways
Itagane et al., Endocrinology 1991
:
The
effects of
IGF-I on
proteoglycan synthesis, as judged by the incorporation of [ 35S ] sulfate, were stimulated by treatment with progesterone or testosterone ... When chondrocytes were treated with hydrocortisone or dexamethasone for 24 h before the addition of IGF-I, the glucocorticoids synergistically accelerated
proteoglycan synthesis
mediated by
IGF-I
Fosang et al., Matrix 1991
:
The
effect of
IGF-1 ( 100 ng/ml ) on matrix degradation of
proteoglycan and its ability to inhibit the effects of IL-1 ( 10 ng/ml ) was also assessed
Abrahamsson et al., J Orthop Res 1991
:
To identify factors with the potential of affecting those abilities, the
effects of recombinant human
insulin-like growth factor ( rhIGF-I ), insulin and fetal calf serum ( FCS ) on the synthesis of
proteoglycan , collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit
Miller et al., Arthritis Rheum 2010
:
We have previously modified IGF-1 by adding a heparin binding domain and have shown that this fusion protein (
HB-IGF-1 )
stimulates sustained
proteoglycan synthesis in cartilage
Yammani et al., Arthritis Res Ther 2012
(MAP Kinase Signaling System) :
Pretreatment of chondrocytes with eNAMPT inhibited
IGF-1 stimulated
proteoglycan synthesis in a dose dependent manner
Tyler et al., Biochem J 1989
:
Insulin-like growth factor 1 can decrease degradation and
promote synthesis of
proteoglycan in cartilage exposed to cytokines ...
IGF 1 induced a dose dependent 2.5-fold stimulation of
proteoglycan synthesis, with a half-maximal dose of 25 ng/ml ... It is concluded that
IGF 1 enhances the synthesis of aggregating
proteoglycan in cartilage exposed to cytokines and can directly decrease both the basal and the cytokine stimulated degradation of proteoglycan in cartilage
Nevo et al., Mech Ageing Dev 1984
:
The
effects of normal human serum,
insulin-like growth factor and beta-D-xyloside on the synthesis of
proteoglycan , as well as their differential effect on the synthesis of chondroitin sulfate and keratan sulfate side-chains, were studied in chick embryonal cartilage
Silbergeld et al., Acta Endocrinol (Copenh) 1981
:
It was postulated that
IGF could
stimulate proteoglycan synthesis not only by elongating existing chondroitin sulphate chains but also by increased synthesis of other sugar chains e.g. keratan sulphate and oligosaccharides
Ohlsson et al., Mol Cell Endocrinol 1993
:
Both GH and insulin-like growth factor-I (IGF-I) stimulated 3H-thymidine uptake in FTC 5 : 3 ( 194 +/- 28 % and 405 +/- 127 % over control, respectively ), while
proteoglycan synthesis, as measured by [ 35S ] sulphate uptake, was
stimulated by
IGF-I only ( 101 +/- 18 % over control ). ( ABSTRACT TRUNCATED AT 250 WORDS )
Verschure et al., J Rheumatol 1994
(Arthritis...) :
Stimulation of
proteoglycan synthesis by triamcinolone acetonide and
insulin-like growth factor 1 in normal and arthritic murine articular cartilage
Rosselot et al., J Bone Miner Res 1994
:
Effect of growth hormone,
insulin-like growth factor I , basic fibroblast growth factor, and transforming growth factor beta on cell proliferation and
proteoglycan synthesis by avian postembryonic growth plate chondrocytes
Lazarus et al., Lymphokine Cytokine Res 1993
:
IL-1 alpha inhibited
IGF-1 stimulated
proteoglycan ( PG ) synthesis > 95 % at 20 ng/ml ( p < 0.01 )
van der Kraan et al., Ann Rheum Dis 1993
:
The in vitro
effect of
IGF-I and steroids on
proteoglycan synthesis in murine patellar cartilage was evaluated by [ 35S ] sulphate incorporation in combination with dissociative gel chromatography using a Sephacryl S-1000 column
van Beuningen et al., Ann Rheum Dis 1993
:
In the
presence of
insulin-like growth factor no extra effect of TGF-beta on
proteoglycan synthesis was observed
Osada et al., J Orthop Res 1996
:
Autocrine/paracrine mechanism of insulin-like growth factor-1 secretion, and the
effect of
insulin-like growth factor-1 on
proteoglycan synthesis in bovine intervertebral discs ... The present study was undertaken to investigate the
effect of
insulin-like growth factor-1 on
proteoglycan synthesis and the autocrine/paracrine mechanism involving insulin-like growth factor-1 in the bovine coccygeal intervertebral disc ...
Insulin-like growth factor-1 stimulated
proteoglycan synthesis in cultured cells of the nucleus pulposus of bovine intervertebral discs in a dose dependent manner, and the effect was inhibited by an anti-insulin-like growth factor-1 monoclonal antibody ... These results suggest that
insulin-like growth factor-1 stimulates
proteoglycan synthesis in cells of the nucleus pulposus and that these cells in culture have an insulin-like growth factor-1 autocrine/paracrine mechanism
Bonassar et al., Exp Cell Res 1997
:
Pretreatment with EGF increased the responsiveness of chondrocytes to IGF-I, resulting in 90 and 60 % augmentation of
IGF-I stimulated mitotic activity and
proteoglycan synthesis, respectively
Morales et al., Arch Biochem Biophys 1997
:
As before, 10 ng/ml TGF-beta or
IGF-1 increased
proteoglycan synthesis and maintained its homeostasis
Takigawa et al., Endocrinology 1997
(Bone Neoplasms...) :
Both insulin-like growth factor
(IGF)-I and IGF-II
increased the synthesis of cartilage-type, large
proteoglycan in a human chondrosarcoma derived chondrocyte cell line, HCS-2/8 ... Both
anti-IGF-IR antibody and anti-IGF-II antibody
inhibited proteoglycan synthesis ... Even in the presence of anti-IGF-IR antibody,
IGF-II and mannose-6-phosphate
stimulated proteoglycan synthesis in the cells
Sunic et al., Endocrinology 1998
:
The aim of this study was to investigate the
effects of
IGF-I and IL-1 on IGFBP production by ovine articular chondrocytes ( OAC ) and the roles of these IGFBPs in the regulation of
proteoglycan synthesis ... Furthermore, the attenuation of
IGF-I stimulated
proteoglycan synthesis by IL-1alpha in OAC appears to be mediated by chondrocyte IGFBPs